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  1. Auburn, Indiana: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Indiana. It falls under Indiana's 3rd congressional district.12 US Recovery Act Smart Grid Projects in Auburn, Indiana City of Auburn, IN Smart Grid Project Utility...

  2. Auburn-Washburn Wind Project | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    to: navigation, search Name Auburn-Washburn Wind Project Facility Auburn-Washburn Sector Wind energy Facility Type Community Wind Owner Auburn-Washburn School District Address...

  3. Auburn, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Auburn, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 38.8965654, -121.0768901 Show Map Loading map... "minzoom":false,"mappingse...

  4. 2014 Race to Zero Student Design Competition: Auburn University...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Profile (Blue) 2014 Race to Zero Student Design Competition: Auburn University Profile (Blue) 2014 Race to Zero Student Design Competition: Auburn University Profile (Blue), as ...

  5. 2014 Race to Zero Student Design Competition: Auburn University...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Profile 2014 Race to Zero Student Design Competition: Auburn University Profile 2014 Race to Zero Student Design Competition: Auburn University Profile, as posted on the U.S. ...

  6. Auburn Hot Spring Pool & Spa Low Temperature Geothermal Facility...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hot Spring Pool & Spa Low Temperature Geothermal Facility Facility Auburn Hot Spring Sector Geothermal energy Type Pool and Spa Location Auburn, Wyoming Coordinates...

  7. Auburn, Washington: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Map This article is a stub. You can help OpenEI by expanding it. Auburn is a city in King County and Pierce County, Washington. It falls under Washington's 8th congressional...

  8. Auburn University | Princeton Plasma Physics Lab

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Auburn University Auburn University Professors J. Hanson and G. Hartwell Plasma equilibrium reconstruction relies on many pin-point magnetic field measurements (~ 100) during the plasma discharge and rather detailed analysis. A widely used analysis code to perform these reconstructions is the VMEC MHD equilibrium code, developed for the 2-D magnetic field configuration of the tokamak device. One complication to the magnetic field measurements within the plasma is compensating for induced

  9. City of Auburn Hills (Text Version) | Department of Energy

    Energy.gov [DOE] (indexed site)

    the process to best plan for electric vehicles in Michigan. ... thing to note is that the car companies can make a great ... Energy Coalition Plug-in Michigan Plan is trying to do. ...

  10. Workplace Charging Challenge Partner: City of Auburn Hills |...

    Office of Environmental Management (EM)

    The City's ordinance encourages, but does not require, developers, builders, homeowners and business owners to make PEV charging stations a regular part of construction. ...

  11. 2014 Race to Zero Student Design Competition: Auburn University Profile |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Profile 2014 Race to Zero Student Design Competition: Auburn University Profile 2014 Race to Zero Student Design Competition: Auburn University Profile, as posted on the U.S. Department of Energy website. rtz_auburn_profile.pdf (7.01 MB) More Documents & Publications 2014 Race to Zero Student Design Competition: University of Minnesota Profile 2014 Race to Zero Student Design Competition: Georgia Institute of Technology

  12. Auburn, New Hampshire: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Auburn, New Hampshire: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 43.0045288, -71.348398 Show Map Loading map... "minzoom":false,"mappings...

  13. City of Auburn, Iowa (Utility Company) | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Iowa (Utility Company) Jump to: navigation, search Name: City of Auburn Place: Iowa Phone Number: (712) 688-2264 Website: www.auburniowa.netindex.php?o Facebook: https:...

  14. Auburn Board of Public Works | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    2010 - File1a" Retrieved from "http:en.openei.orgwindex.php?titleAuburnBoardofPublicWorks&oldid879873" Feedback Contact needs updating Image needs updating...

  15. City of Auburn, Indiana (Utility Company) | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Activity Distribution Yes This article is a stub. You can help OpenEI by expanding it. City of Auburn, IN Smart Grid Project was awarded 2,075,080 Recovery Act Funding with a...

  16. Auburn low-temperature geothermal well. Volume 6. Final report

    SciTech Connect

    Lynch, R.S.; Castor, T.P.

    1983-12-01

    The Auburn well was drilled to explore for low temperature geothermal resources in central New York State. The Auburn site was selected based on: its proximity to the Cayuga County anomaly (30/sup 0/C/km), its favorable local geological conditions and the potential to provide hot water and space heating to two educational facilities. The well was drilled to a total depth of 5250 feet and into the Pre-Cambrian Basement. The well was extensively logged, flow and stress tested, hydraulically stimulated, and pump (pressure transient analysis) tested. The low-temperature geothermal potential was assessed in terms of: geological environment; hydrological conditions; reservoir characteristics; and recoverable hydrothermal reserves. The average geothermal gradient was measured to be as high as 26.7/sup 0/C/km with a bottom-hole temperature of 126/sup 0/ +- 1/sup 0/F. The proved volumetric resources were estimated to be 3.0 x 10/sup 6/ stock tank barrels (STB) with a maximum initial deliverability of approx.11,600 STB/D and a continuous deliverability of approx.3400 STB/D. The proved hydrothermal reserves were estimated to be 21.58 x 10/sup 10/ Btu based on a volumetric component (4.13 x 10/sup 10/ Btu), and a reinjection component (17.45 x 10/sup 10/ Btu). The conclusion was made that the Auburn low-temperature reservoir could be utilized to provide hot water and space heating to the Auburn School District.

  17. 2014 Race to Zero Student Design Competition: Auburn University Profile (Blue)

    Energy.gov [DOE]

    2014 Race to Zero Student Design Competition: Auburn University Profile (Blue), as posted on the U.S. Department of Energy website.

  18. Uni-Solar | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Type Test & Evaluation Partner Partnering Center within NREL National Center for Photovoltaics Uni-Solar is a company located in Auburn Hills, MI. References "Uni-Solar"...

  19. Guardian Industries | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Industries Jump to: navigation, search Name: Guardian Industries Place: Auburn Hills, MI Website: www.guardian.com References: Results of NREL Testing (Glass Magazine)1 Guardian...

  20. Workplace Charging Challenge Partner: FCA US LLC | Department...

    Office of Environmental Management (EM)

    Hills, MI Domestic Employees: 44,000 FCA US LLC currently has six plug-in electric vehicle (PEV) charging stations available for employee use at its Auburn Hills headquarters. ...

  1. Samson Hille

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Samson Hille About ESnet Our Mission The Network ESnet History Governance & Policies Career Opportunities ESnet Staff & Org Chart ESnet Leadership Administration Advanced Network Technologies Cybersecurity Infrastructure, Identity & Collaboration Network Engineering Network Planning Operational Enhancements Office of the CTO Science Engagement Tools Team Contact Us Contact Us Technical Assistance: 1 800-33-ESnet (Inside US) 1 800-333-7638 (Inside US) 1 510-486-7600 (Globally) 1

  2. Record Hill | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Record Hill Jump to: navigation, search Name Record Hill Facility Record Hill Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner Wagner Wind...

  3. RECORD HILL | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    RECORD HILL RECORD HILL DOE-LPO_Project-Posters_WIND_Record-Hill.pdf (385.07 KB) More Documents & Publications KAHUKU GRANITE RELIABLE SHEPHERDS FLAT

  4. Geophysical exploration and hydrologic impact of the closed Gracelawn landfill in Auburn, ME

    SciTech Connect

    Wisniewski, D. . Geology Dept.)

    1993-03-01

    Several geophysical methods were used over portions of the Gracelawn landfill, in Auburn, Maine to determine the surface boundaries and subsurface structure of this closed landfill, and to determine the landfill's effects on groundwater quality. The landfill was originally a sand and gravel pit excavated in the 1950's and early 1960's, and was used as a landfill from 1964--1977. The site is unlined, has a clay cap, and has been graded and developed as a baseball park. Two seismic refraction lines were performed to obtain a minimum depth to bedrock of 80 m. Seismic velocities of methane gas-saturated trash ranged from 250 to 340 m/s, and sand velocities are approximately 800 m/s. Two electrical resistivity Wenner surveys over the trash yielded the depth to saturated material and thickness of the trash layers. Resistivity values for dry refuse ranged from 1,000-2,000 [Omega]*m. A third electrical resistivity survey yielded the thickness of unsaturated and saturated sands bordering the landfill. Dry sands were found to have a resistivity of 1,000 [Omega]*m, and saturated sands a resistivity of 500 [Omega]*m. Gravity and magnetic survey grids across the site revealed anomalies which were mapped to illustrate the irregular morphology of the buried trash as well as its surface boundaries. Residual magnetic anomalies are on the order of 2,000 nT. Residual gravity anomalies are up to 5 mGal. Groundwater elevations determined by the geophysical survey, combined with a survey of existing water monitoring well logs, indicate that the groundwater flow in the sand and gravel aquifer is to the southeast, away from the public water supply, Lake Auburn, which lies to the north of the site. However, correlations between the bedrock fracture analysis and the geophysical survey illustrate that there is potential for contamination of Lake Auburn via the bedrock aquifer.

  5. BPA-2015-01481-FOIA Request

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    that authorizes the withholding of the records. Finally, as an accommodation, I ask that you reply electronically by email. Friedman PO Box 215077 Auburn Hills, MI 48321-5077...

  6. Electron-ion hybrid instability experiment upgrades to the Auburn Linear Experiment for Instability Studies

    SciTech Connect

    DuBois, A. M.; Arnold, I.; Thomas, E. Jr.; Tejero, E.; Amatucci, W. E.

    2013-04-15

    The Auburn Linear EXperiment for Instability Studies (ALEXIS) is a laboratory plasma physics experiment used to study spatially inhomogeneous flows in a magnetized cylindrical plasma column that are driven by crossed electric (E) and magnetic (B) fields. ALEXIS was recently upgraded to include a small, secondary plasma source for a new dual source, interpenetrating plasma experiment. Using two plasma sources allows for highly localized electric fields to be made at the boundary of the two plasmas, inducing strong E Multiplication-Sign B velocity shear in the plasma, which can give rise to a regime of instabilities that have not previously been studied in ALEXIS. The dual plasma configuration makes it possible to have independent control over the velocity shear and the density gradient. This paper discusses the recent addition of the secondary plasma source to ALEXIS, as well as the plasma diagnostics used to measure electric fields and electron densities.

  7. UNC Chapel Hill

    SciTech Connect

    2009-10-01

    This is a combined heat and power (CHP) project profile on a 32 MW cogeneration plant at UNC Chapel Hill in North Carolina.

  8. Hill Air Force Base

    Energy.gov [DOE]

    Energy savings performance contracting at Hill Air Force Base generated much interest during a recent training session on energy management that downlinked 12 Department of Defense sites. Energy...

  9. Hills Inc | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hills Inc Jump to: navigation, search Name: Hills Inc Region: United States Sector: Marine and Hydrokinetic Website: http: This company is listed in the Marine and Hydrokinetic...

  10. Graham Hill | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Graham Hill About Us Graham Hill - Research Analyst, National Renewable Energy Lab Most Recent Developer Resources for Apps for Energy April 4

  11. Science on the Hill

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science on the Hill Science on the Hill Recent Los Alamos research and achievements featured in the Santa Fe New Mexican News Releases Science Briefs Photos Picture of the Week Publications Social Media Videos Fact Sheets This 3D image taken at Los Alamos National Laboratory by x-ray tomography shows an experimental fuel cell membrane electrode assembly. On track for a clean, hydrogen-powered future Los Alamos, within the ElectroCat consortium, is investigating less expensive, more abundant

  12. Campbell Hill Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill Wind Farm Jump to: navigation, search Name Campbell Hill Wind Farm Facility Campbell Hill Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  13. Rolling Hills (IA) | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Rolling Hills (IA) Jump to: navigation, search Name Rolling Hills (IA) Facility Rolling Hills (IA) Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  14. Crofton Hills Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Crofton Hills Wind Farm Jump to: navigation, search Name Crofton Hills Wind Farm Facility Crofton Hills Sector Wind energy Facility Type Commercial Scale Wind Facility Status In...

  15. Union Hill-Novelty Hill, Washington: Energy Resources | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    help OpenEI by expanding it. Union Hill-Novelty Hill is a census-designated place in King County, Washington.1 References US Census Bureau 2005 Place to 2006 CBSA...

  16. RECORD HILL | Department of Energy

    Energy.gov [DOE] (indexed site)

    RECORD HILL PROJECT SUMMARY In August 2011, the Department of Energy issued a 102 million loan guarantee to finance Record Hill, a 51-MW wind power generation project and an eight ...

  17. Fenton Hill Hdr Area | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill Hdr Area Redirect page Jump to: navigation, search REDIRECT Fenton Hill Hdr Geothermal Area Retrieved from "http:en.openei.orgwindex.php?titleFentonHillHdrArea&oldid...

  18. Hills Creek Powerhouse Turbine and Unit Rehabilitation

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Hills Creek Powerhouse Turbine and Unit Rehabilitation This project will replace the runners and windings for the two 17.5 MW units at the Hills Creek powerhouse. Hills Creek is a...

  19. Red Hills Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hills Wind Farm Jump to: navigation, search Name Red Hills Wind Farm Facility Red Hills Wind Farm Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  20. Long Hill Energy Ltd | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill Energy Ltd Jump to: navigation, search Name: Long Hill Energy Ltd Place: United Kingdom Sector: Wind energy Product: JV formed by Snowmountain Eneterprises Ltd and Wind Direct...

  1. Blue Hill Partners LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Partners LLC Jump to: navigation, search Logo: Blue Hill Partners LLC Name: Blue Hill Partners LLC Address: 40 W. Evergreen Ave. Place: Philadelphia, Pennsylvania Zip: 19118...

  2. Science on the Hill: Methane cloud hunting

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Methane cloud hunting Science on the Hill: Methane cloud hunting Los Alamos researchers go ... Science on the Hill: Methane cloud hunting When our team from Los Alamos National ...

  3. Sou Hills Geothermal Project | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Sou Hills Geothermal Project Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Development Project: Sou Hills Geothermal Project Project Location Information Coordinates...

  4. Sunny Hill Energy | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill Energy Jump to: navigation, search Name: Sunny Hill Energy Place: San Jose, California Zip: 95113 Sector: Solar Product: California-based solar financing and business support...

  5. Loess Hills Wind Energy Center | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Loess Hills Wind Energy Center Jump to: navigation, search Name Loess Hills Wind Energy Center Facility Loess Hills Wind Energy Center Sector Wind energy Facility Type Commercial...

  6. Settlers Hill Gas Recovery Biomass Facility | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Settlers Hill Gas Recovery Biomass Facility Jump to: navigation, search Name Settlers Hill Gas Recovery Biomass Facility Facility Settlers Hill Gas Recovery Sector Biomass Facility...

  7. Mars Hill (2006) Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Mars Hill (2006) Wind Farm Jump to: navigation, search Name Mars Hill (2006) Wind Farm Facility Mars Hill (2006) Sector Wind energy Facility Type Commercial Scale Wind Facility...

  8. Puente Hills Energy Recovery Biomass Facility | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    Puente Hills Energy Recovery Biomass Facility Jump to: navigation, search Name Puente Hills Energy Recovery Biomass Facility Facility Puente Hills Energy Recovery Sector Biomass...

  9. DOE Tour of Zero Floorplans: Candlewood Hills Rebuild by BPC...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Candlewood Hills Rebuild by BPC Green Builders DOE Tour of Zero Floorplans: Candlewood Hills Rebuild by BPC Green Builders DOE Tour of Zero Floorplans: Candlewood Hills Rebuild by ...

  10. Spittal Hill Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Spittal Hill Wind Farm Jump to: navigation, search Name: Spittal Hill Wind Farm Place: United Kingdom Sector: Wind energy Product: Set up to manage wind projects in the Scotland....

  11. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    SciTech Connect

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  12. Chattahoochee Hill Country, Georgia: Energy Resources | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill Country, Georgia: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 31.721548, -83.2599068 Show Map Loading map... "minzoom":false,"mappings...

  13. Arbor Hills Biomass Facility | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    NEEDS 2006 Database Retrieved from "http:en.openei.orgwindex.php?titleArborHillsBiomassFacility&oldid397151" Feedback Contact needs updating Image needs updating...

  14. Black Hills Energy (Electric) - Residential Energy Efficiency...

    Energy.gov [DOE] (indexed site)

    Water Heater: 450 Refrigerator: 30unit Freezer: 30unit Dishwasher: 30unit Television: 25unit CFLLED Bulbs: In-store rebates Summary Black Hills Energy (BHE) offers...

  15. Hill Air Force Base | Department of Energy

    Energy Saver

    ... Competition for Department of Defense (DOD) Project Funds Overcome: In recent years, Hill ... to compete for scarce project funds from DOD. Utility rebates fund 50 percent of the ...

  16. Smoky Hills II Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Smoky Hills II Wind Farm Jump to: navigation, search Name Smoky Hills II Wind Farm Facility Smoky Hills II Sector Wind energy Facility Type Commercial Scale Wind Facility Status In...

  17. Dutch Hill/Cohocton Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Dutch HillCohocton Wind Farm Jump to: navigation, search Name Dutch HillCohocton Wind Farm Facility Dutch HillCohocton Wind Farm Sector Wind energy Facility Type Commercial...

  18. McGraw Hill Construction | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    McGraw Hill Construction Jump to: navigation, search Name: McGraw Hill Construction Place: New York, NY Website: www.mcgrawhillconstruction.com References: McGraw Hill...

  19. EA-1824: Record Hill Wind Project in Roxbury, ME | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    4: Record Hill Wind Project in Roxbury, ME EA-1824: Record Hill Wind Project in Roxbury, ME July 1, 2011 EA-1824: Final Environmental Assessment Loan Guarantee to Record Hill Wind,...

  20. Golden Hills Solar Power Plant | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hills Solar Power Plant Facility Golden Hills Solar Sector Solar Facility Type Photovoltaic Developer PowerWorks Location Alameda County, California Coordinates 37.6016892,...

  1. EA-1967: Hills Creek-Lookout Point Transmission Line Rebuild...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    967: Hills Creek-Lookout Point Transmission Line Rebuild, Lane County, Oregon EA-1967: Hills Creek-Lookout Point Transmission Line Rebuild, Lane County, Oregon Summary Bonneville ...

  2. Whitewater Hill Wind Farm II | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    II Jump to: navigation, search Name Whitewater Hill Wind Farm II Facility Whitewater Hill Sector Wind energy Facility Type Community Wind Facility Status In Service Owner Cannon...

  3. LPO5-002-Proj-Poster-WIND-RecordHill

    Office of Environmental Management (EM)

    RECORD HILL Robust turbine technologies at Record Hill allow more power output during extreme weather conditions than conventional wind energy technology. INVESTING in AMERICAN ...

  4. Blue Hill Investment Partners LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill Investment Partners LLC Jump to: navigation, search Name: Blue Hill Investment Partners LLC Place: Philadelphia, Pennsylvania Zip: PA 19118 Sector: Renewable Energy Product: A...

  5. City of Olive Hill, Kentucky (Utility Company) | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    City of Olive Hill, Kentucky (Utility Company) Jump to: navigation, search Name: Olive Hill City of Place: Kentucky Phone Number: (606) 286-2192 Website: www.cityofolivehillutiliti...

  6. City of Rock Hill, South Carolina (Utility Company) | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, South Carolina (Utility Company) Jump to: navigation, search Name: City of Rock Hill Place: South Carolina Phone Number: 803-325-2500 Website: www.cityofrockhill.comdepartm...

  7. City of Blue Hill, Nebraska (Utility Company) | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    Blue Hill, Nebraska (Utility Company) Jump to: navigation, search Name: Blue Hill Municipal Power Place: Nebraska Phone Number: 402.756.3771 Website: bluehillne.comcommunity.php...

  8. Independent Oversight Review, Hanford Site CH2M Hill Plateau...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    CH2M Hill Plateau Remediation Company - November 2012 Independent Oversight Review, Hanford Site CH2M Hill Plateau Remediation Company - November 2012 November 2012 Review of the...

  9. MHK Technologies/Davidson Hill Venturi DHV Turbine | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    Davidson Hill Venturi DHV Turbine < MHK Technologies Jump to: navigation, search << Return to the MHK database homepage Davidson Hill Venturi DHV Turbine.jpg Technology Profile...

  10. Isotopic Analysis At Fenton Hill Hdr Geothermal Area (Goff, Et...

    OpenEI (Open Energy Information) [EERE & EIA]

    Isotopic Analysis At Fenton Hill Hdr Geothermal Area (Goff, Et Al., 1981) Redirect page Jump to: navigation, search REDIRECT Isotopic Analysis- Fluid At Fenton Hill Hdr Geothermal...

  11. Singaraya Hills Green Power Pvt Ltd | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Singaraya Hills Green Power Pvt Ltd Jump to: navigation, search Name: Singaraya Hills Green Power Pvt. Ltd. Place: Vijayawada, Andhra Pradesh, India Zip: 520 010 Sector: Biomass...

  12. Rolling Hills Electric Coop | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    search Name: Rolling Hills Electric Coop Place: Kansas Phone Number: Ellsworth Office (785) 472-4021 or (800) 530-5572 Mankato Office (785) 378-3151 or (877) 906-5903...

  13. Energy Innovation Hub Directors Visit the Hill

    Office of Energy Efficiency and Renewable Energy (EERE)

    Yesterday, the directors of the Energy Department's Energy Innovation Hubs joined policymakers on Capitol Hill to discuss the critical research being conducted at the hubs and to answer questions from attendees.

  14. Bunker Hill Sediment Characterization Study

    SciTech Connect

    Neal A. Yancey; Debby F. Bruhn

    2009-12-01

    The long history of mineral extraction in the Coeur d’Alene Basin has left a legacy of heavy metal laden mine tailings that have accumulated along the Coeur d’Alene River and its tributaries (U.S. Environmental Protection Agency, 2001; Barton, 2002). Silver, lead and zinc were the primary metals of economic interest in the area, but the ores contained other elements that have become environmental hazards including zinc, cadmium, lead, arsenic, nickel, and copper. The metals have contaminated the water and sediments of Lake Coeur d’Alene, and continue to be transported downstream to Spokane Washington via the Spokane River. In 1983, the EPA listed the Bunker Hill Mining and Metallurgical Complex on the National Priorities List. Since that time, many of the most contaminated areas have been stabilized or isolated, however metal contaminants continue to migrate through the basin. Designation as a Superfund site causes significant problems for the economically depressed communities in the area. Identification of primary sources of contamination can help set priorities for cleanup and cleanup options, which can include source removal, water treatment or no action depending on knowledge about the mobility of contaminants relative to water flow. The mobility of contaminant mobility under natural or engineered conditions depends on multiple factors including the physical and chemical state (or speciation) of metals and the range of processes, some of which can be seasonal, that cause mobilization of metals. As a result, it is particularly important to understand metal speciation (National Research Council, 2005) and the link between speciation and the rates of metal migration and the impact of natural or engineered variations in flow, biological activity or water chemistry.

  15. Los Alamos National Laboratory Investigates Fenton Hill to Support...

    Office of Environmental Management (EM)

    Investigates Fenton Hill to Support Future Land Use Los Alamos National Laboratory Investigates Fenton Hill to Support Future Land Use July 29, 2014 - 12:00pm Addthis Sampling...

  16. PP-118 Hill County Electric Cooperative Inc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    PDF icon PP-118 Hill County Electric Cooperative Inc More Documents & Publications EA-118 Hill County Electric Cooperative, Inc. PP-11-2 Fraser Papers Inc PP-89-1 Bangor Hydro-Elec...

  17. EA-118 Hill County Electric Cooperative, Inc. | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    energy to Canada. EA-118 Hill County.pdf More Documents & Publications PP-118 Hill County Electric Cooperative Inc EA-209 Cargill-Alliant, LLC EA-211 DTE Energy Trading, Inc...

  18. Hammars Hill Energy HHE Ltd | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hammars Hill Energy HHE Ltd Jump to: navigation, search Name: Hammars Hill Energy (HHE) Ltd Place: Scotland, United Kingdom Sector: Wind energy Product: UK-based wind power project...

  19. City of Hill City, Kansas (Utility Company) | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill City, Kansas (Utility Company) Jump to: navigation, search Name: City of Hill City Place: Kansas Phone Number: (785) 421-3438 Website: www.discoverhillcity.comBusin Outage...

  20. Mars Hill (2007) Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    7) Wind Farm Jump to: navigation, search Name Mars Hill (2007) Wind Farm Facility Mars Hill (2007) Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  1. Hill County Electric Coop, Inc | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill County Electric Coop, Inc Jump to: navigation, search Name: Hill County Electric Coop, Inc Place: Montana Phone Number: 1-877-394-7804 Website: hcelectric.com Facebook:...

  2. City of Rich Hill, Missouri (Utility Company) | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Missouri (Utility Company) Jump to: navigation, search Name: City of Rich Hill Place: Missouri Phone Number: 417-395-4211 or 417-395-2223 Website: www.richhillmo.com...

  3. Ben Hill County, Georgia: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Climate Zone Number 3 Climate Zone Subtype A. Places in Ben Hill County, Georgia Fitzgerald, Georgia Retrieved from "http:en.openei.orgwindex.php?titleBenHillCounty,Geo...

  4. Tracer Testing At Fenton Hill HDR Geothermal Area (Callahan,...

    OpenEI (Open Energy Information) [EERE & EIA]

    the Hot Dry Rock Geothermal System, Fenton Hill, New Mexico- Tracer Test Results Donald Brown, Robert DuTeaux (1997) Three Principal Results from Recent Fenton Hill Flow Testing...

  5. EA-118 Hill County Electric Cooperative, Inc. | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    to Canada. EA-118 Hill County.pdf (13.91 KB) More Documents & Publications PP-118 Hill County Electric Cooperative Inc EA-209 Cargill-Alliant, LLC EA-211 DTE Energy Trading, Inc

  6. WilderHill New Energy Finance LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Kingdom Product: Joint venture set up to publish the WilderHill New Energy Global Innovation Index (NEX). References: WilderHill New Energy Finance LLC1 This article is a...

  7. Enforcement Letter, CH2M Hill- October 4, 2004

    Energy.gov [DOE]

    Issued to CH2M Hill related to at a Lapse in Dosimetry Accreditation at the Separations Process Research Unit

  8. CH2M HILL Plateau Remediation Company - Hanford Site

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Contracting CH2M HILL Plateau Remediation Company Contracting ORP Contracts and Procurements RL Contracts and Procurements CH2M HILL Plateau Remediation Company Mission Support Alliance Washington Closure Hanford HPM Corporation (HPMC) Wastren Advantage, Inc. Bechtel National, Inc. Washington River Protection Solutions CH2M HILL Plateau Remediation Company Email Email Page | Print Print Page | Text Increase Font Size Decrease Font Size CH2M CH2M HILL Plateau Remediation Company is the prime

  9. Nuclear Energy Technologies Robert Hill | Argonne National Laboratory

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Nuclear Energy Technologies Robert Hill Share Description Nuclear engineer Robert Hill discusses Argonne's continued innovation in the development of advanced nuclear energy systems. Speakers Robert Hill Duration 1:36 Topic Energy Energy sources Nuclear energy Browse By - Any - General Argonne Information Energy -Energy efficiency --Vehicles ---Alternative fuels ---Automotive engineering ---Diesel ---Electric drive technology ---Hybrid & electric vehicles ---Hydrogen & fuel cells

  10. US ENC MI Site Consumption

    Energy Information Administration (EIA) (indexed site)

    MI Site Consumption million Btu $0 $500 $1,000 $1,500 $2,000 $2,500 US ENC MI Expenditures dollars ALL ENERGY average per household (excl. transportation) 0 2,000 4,000 6,000 8,000 10,000 12,000 US ENC MI Site Consumption kilowatthours $0 $250 $500 $750 $1,000 $1,250 $1,500 US ENC MI Expenditures dollars ELECTRICITY ONLY average per household * Michigan households use 123 million Btu of energy per home, 38% more than the U.S. average. * High consumption, combined with low costs for heating fuels

  11. EA-1581: Sand Hills Wind Project, Wyoming

    Energy.gov [DOE]

    The Bureau of Land Management, with DOE’s Western Area Power Administration as a cooperating agency, was preparing this EA to evaluate the environmental impacts of a proposal to construct, operate, and maintain the Sand Hills Wind Energy Facility on private and federal lands in Albany County, Wyoming. If the proposed action had been implemented, Western would have interconnected the proposed facility to an existing transmission line. This project has been canceled.

  12. Auburn University | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Alabama Zip: 36849 Product: Largest university in Alabama, enrolling approximately 23,000 students in 230 undergraduate, graduate, and professional programs. References:...

  13. Workplace Charging Challenge Partner: Volkswagen Group of America |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Volkswagen Group of America Workplace Charging Challenge Partner: Volkswagen Group of America Workplace Charging Challenge Partner: Volkswagen Group of America Joined the Challenge: July 2014 Headquarters: Herndon, VA Charging Locations: Herndon, VA; Oxnard, CA; Belmont, CA; Allendale, NJ; Maricopa, AZ; Auburn Hills, MI; Golden, CO; Clearwater, FL; Buffalo Grove, IL; Davie, FL; Irving, TX; Livermore, CA; Atlanta, GA; Dublin, OH; Marlborough, MA; Renton, WA; Santa Monica,

  14. US ENC MI Site Consumption

    Annual Energy Outlook

    ... Yes Yes No No 0% 20% 40% 60% 80% 100% US MI No Car CAR IS PARKED WITHIN 20 FT OF ELECTRICAL OUTLET More highlights from RECS on housing characteristics and energy-related ...

  15. Voluntary Protection Program Onsite Review, CH2M HILL Plateau...

    Energy.gov [DOE] (indexed site)

    Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment Voluntary Protection Program Onsite Review, CH2M HILL Analytical Technical...

  16. Surface Gas Sampling At Fenton Hill HDR Geothermal Area (Grigsby...

    OpenEI (Open Energy Information) [EERE & EIA]

    to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Surface Gas Sampling At Fenton Hill HDR Geothermal Area (Grigsby, Et Al., 1983) Exploration...

  17. Development Wells At Fenton Hill HDR Geothermal Area (Dreesen...

    OpenEI (Open Energy Information) [EERE & EIA]

    Dreesen, Et Al., 1987) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Development Wells At Fenton Hill HDR Geothermal Area (Dreesen, Et Al.,...

  18. Fenton Hill HDR Geothermal Area | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    HDR Geothermal Area (Heiken & Goff, 1983) Data Acquisition-Manipulation 1983 Hot Dry Rock Geothermal Energy In The Jemez Volcanic Field, New Mexico Development Wells At Fenton Hill...

  19. Morgan Hill, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 37.1305012, -121.6543901 Show Map Loading map... "minzoom":false,"mappingserv...

  20. Crest Hill, Illinois: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Illinois: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.554753, -88.0986709 Show Map Loading map... "minzoom":false,"mappingservice"...

  1. Cockrell Hill, Texas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Cockrell Hill, Texas: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 32.7362421, -96.8869481 Show Map Loading map... "minzoom":false,"mappings...

  2. Clover Hill, Maryland: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Maryland: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.4562128, -77.4288745 Show Map Loading map... "minzoom":false,"mappingservice...

  3. Pine Hill, New York: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Pine Hill, New York: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 43.3075694, -75.6521271 Show Map Loading map... "minzoom":false,"mappingse...

  4. Bay Hill, Florida: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Florida: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 28.4680604, -81.5161839 Show Map Loading map... "minzoom":false,"mappingservice"...

  5. Brewster Hill, New York: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, New York: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.423983, -73.6042939 Show Map Loading map... "minzoom":false,"mappingservice"...

  6. The Village of Indian Hill, Ohio: Energy Resources | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    Village of Indian Hill, Ohio: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.180136, -84.347958 Show Map Loading map......

  7. North College Hill, Ohio: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    College Hill, Ohio: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.2183911, -84.5507778 Show Map Loading map... "minzoom":false,"mappingser...

  8. Waite Hill, Ohio: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Waite Hill, Ohio: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.6186592, -81.3840001 Show Map Loading map... "minzoom":false,"mappingservi...

  9. Valley Hill, North Carolina: Energy Resources | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, North Carolina: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 35.2984505, -82.4831755 Show Map Loading map... "minzoom":false,"mappings...

  10. Sugar Hill, New Hampshire: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, New Hampshire: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 44.215341, -71.799532 Show Map Loading map... "minzoom":false,"mappingserv...

  11. Mint Hill, North Carolina: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Mint Hill, North Carolina: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 35.1795892, -80.6472904 Show Map Loading map... "minzoom":false,"map...

  12. Fruit Hill, Ohio: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Fruit Hill, Ohio: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.0756169, -84.3643835 Show Map Loading map... "minzoom":false,"mappingservi...

  13. West Hill, Ohio: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Ohio: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.2328339, -80.5192377 Show Map Loading map... "minzoom":false,"mappingservice":"g...

  14. Humboldt Hill, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 40.7259606, -124.1897819 Show Map Loading map... "minzoom":false,"mappingserv...

  15. Quartz Hill, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Quartz Hill, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 34.6452645, -118.2181308 Show Map Loading map... "minzoom":false,"mapp...

  16. Cumberland Hill, Rhode Island: Energy Resources | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Rhode Island: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.9745431, -71.4670043 Show Map Loading map... "minzoom":false,"mappingser...

  17. Liberty Hill, Texas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, Texas: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 30.5149244, -95.1435483 Show Map Loading map... "minzoom":false,"mappingservice":"...

  18. Vine Hill, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hill, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 38.0085326, -122.0960753 Show Map Loading map... "minzoom":false,"mappingserv...

  19. HDR Geothermal Energy: Important Lessons From Fenton Hill

    National Nuclear Security Administration (NNSA)

    2009 SGP-TR-187 HOT DRY ROCK GEOTHERMAL ENERGY: IMPORTANT LESSONS FROM FENTON HILL ... concept of Hot Dry Rock (HDR) geothermal energy originated at Los Alamos National ...

  20. Forest Hills, Pennsylvania: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Forest Hills, Pennsylvania: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 40.4197911, -79.8500487 Show Map Loading map......

  1. Forest Hills, Michigan: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Forest Hills, Michigan: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 42.9594739, -85.4897456 Show Map Loading map... "minzoom":false,"mappin...

  2. Isotopic Analysis- Fluid At Fenton Hill HDR Geothermal Area ...

    OpenEI (Open Energy Information) [EERE & EIA]

    Interactions in the Fenton Hill, New Mexico, Hot Dry Rock Geothermal Systems I. Fluid Mixing and Chemical Geothermometry Additional References Retrieved from "http:...

  3. Compound and Elemental Analysis At Fenton Hill HDR Geothermal...

    OpenEI (Open Energy Information) [EERE & EIA]

    Interactions in the Fenton Hill, New Mexico, Hot Dry Rock Geothermal Systems I. Fluid Mixing and Chemical Geothermometry Additional References Retrieved from "http:...

  4. Chapel Hill, North Carolina: Energy Resources | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    district.12 Registered Energy Companies in Chapel Hill, North Carolina Practical Eco University of North Carolina References US Census Bureau Incorporated place and...

  5. Rochester Hills, Michigan: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Companies in Rochester Hills, Michigan Energy Conversion Devices Inc aka ECD Ovonics Luma Resources LLC Ovonic Hydrogen Systems LLC formerly Texaco Ovonic Hydrogen Systems LLC...

  6. Seven Mile Hill Wind Farm | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    www.wsgs.uwyo.eduTopicsEnergyResourceswind.aspx http:renewableenergydev.comredwind-power-seven-mile-hill-wind-energy-project Retrieved from "http:en.openei.orgw...

  7. Conceptual Model At Fenton Hill HDR Geothermal Area (Goff, Et...

    OpenEI (Open Energy Information) [EERE & EIA]

    Goff, Et Al., 1988) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Conceptual Model At Fenton Hill HDR Geothermal Area (Goff, Et Al., 1988)...

  8. Surface Gas Sampling At Fenton Hill HDR Geothermal Area (Goff...

    OpenEI (Open Energy Information) [EERE & EIA]

    Goff & Janik, 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Surface Gas Sampling At Fenton Hill HDR Geothermal Area (Goff & Janik, 2002)...

  9. Compound and Elemental Analysis At Fenton Hill HDR Geothermal...

    OpenEI (Open Energy Information) [EERE & EIA]

    Goff & Janik, 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Compound and Elemental Analysis At Fenton Hill HDR Geothermal Area (Goff &...

  10. Modeling-Computer Simulations At Fenton Hill HDR Geothermal Area...

    OpenEI (Open Energy Information) [EERE & EIA]

    navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Modeling-Computer Simulations At Fenton Hill HDR Geothermal Area (Brown & DuTeaux, 1997) Exploration...

  11. Modeling-Computer Simulations At Fenton Hill HDR Geothermal Area...

    OpenEI (Open Energy Information) [EERE & EIA]

    navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Modeling-Computer Simulations At Fenton Hill HDR Geothermal Area (Goff & Decker, 1983) Exploration...

  12. Science on the Hill: Turning windows into solar panels

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science on the Hill: Turning windows into solar panels Turning windows into solar panels Working with quantum dots, researchers achieve a breakthrough in solar-concentrating ...

  13. Conceptual Model At Fenton Hill HDR Geothermal Area (Grigsby...

    OpenEI (Open Energy Information) [EERE & EIA]

    the Fenton Hill, New Mexico, Hot Dry Rock Geothermal Systems II. Modeling Geochemical Behavior Additional References Retrieved from "http:en.openei.orgwindex.php?titleConceptu...

  14. El Dorado Hills, California: Energy Resources | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    Dorado Hills, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 38.6857367, -121.082167 Show Map Loading map... "minzoom":false,"mapp...

  15. Science on the Hill: Quenching New Mexico's thirst with brackish...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Quenching New Mexico's thirst with brackish water Science on the Hill: Quenching New ... Brackish, or salty, water may prove an invaluable liquid resource for New Mexico. Science ...

  16. Black Hills Energy (Gas) - Commercial Energy Efficiency Rebate...

    Energy.gov [DOE] (indexed site)

    sq ft. Infiltration Control: 70% of installed cost Doors: 25 or 50 Pool Cover: 250 Spa Cover: 50 Summary Black Hills Energy offers commercial and industrial customers...

  17. Enforcement Letter, Kaiser-Hill Company, LLC- August 12, 2004

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC related to a Water Treatment System Breach and Foam Fire at the Rocky Flats Environmental Technology Site

  18. View Park-Windsor Hills, California: Energy Resources | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    Park-Windsor Hills, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 33.9929545, -118.3491169 Show Map Loading map......

  19. South San Jose Hills, California: Energy Resources | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jose Hills, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 34.0127894, -117.9047845 Show Map Loading map... "minzoom":false,"mappi...

  20. Flow Test At Fenton Hill HDR Geothermal Area (Brown, 1995) |...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Brown, 1995) Exploration Activity Details Location...

  1. Flow Test At Fenton Hill HDR Geothermal Area (Callahan, 1996...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Callahan, 1996) Exploration Activity Details...

  2. Flow Test At Fenton Hill HDR Geothermal Area (Brown, 1994) |...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Brown, 1994) Exploration Activity Details Location...

  3. Desert Hills, Arizona: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Desert Hills, Arizona: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 34.5538996, -114.3724569 Show Map Loading map... "minzoom":false,"mappin...

  4. Black Hills Energy (Electric)- Commercial Energy Efficiency Program

    Energy.gov [DOE]

    Black Hills Energy also offers the custom rebate program to commercial and industrial customers for projects that reduce energy usage. Eligible projects include chillers, unitary HVAC equipment,...

  5. Geothermal Literature Review At Fenton Hill HDR Geothermal Area...

    OpenEI (Open Energy Information) [EERE & EIA]

    navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal Literature Review At Fenton Hill HDR Geothermal Area (Goff & Decker, 1983) Exploration Activity...

  6. MEMORANDUM TO: File FROM: David R. Hill RE: Meeting Concerning...

    Office of Environmental Management (EM)

    MEMORANDUM TO: File FROM: David R. Hill RE: Meeting Concerning Potential Test Procedures ... with DOE officials concerning potential test procedures and energy conservation ...

  7. Painted Hills B&C Wind Farm I | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Painted Hills B&C Wind Farm I Jump to: navigation, search Name Painted Hills B&C Wind Farm I Facility Painted Hills B&C Sector Wind energy Facility Type Commercial Scale Wind...

  8. DOE Tour of Zero Floorplans: Vision Hill Lot 1 by Mandalay Homes...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Vision Hill Lot 1 by Mandalay Homes DOE Tour of Zero Floorplans: Vision Hill Lot 1 by Mandalay Homes DOE Tour of Zero Floorplans: Vision Hill Lot 1 by Mandalay Homes

  9. Real-time sub-<mi>>ngstrom...

    Office of Scientific and Technical Information (OSTI)

    Real-time sub-<mi>>ngstrom imaging of reversible and irreversible conformations in rhodium catalysts and graphene Kisielowski, Christian; Wang,...

  10. Massive Hydraulic Fracture of Fenton Hill HDR Well EE-3 | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    Activities Activities (1) Micro-Earthquake At Fenton Hill HDR Geothermal Area (Brown, 2009) Areas (1) Fenton Hill HDR Geothermal Area Regions (0) Retrieved from "http:...

  11. Water-Gas Samples At Fenton Hill Hdr Geothermal Area (Goff &...

    OpenEI (Open Energy Information) [EERE & EIA]

    Water-Gas Samples At Fenton Hill Hdr Geothermal Area (Goff & Janik, 2002) Redirect page Jump to: navigation, search REDIRECT Surface Gas Sampling At Fenton Hill Hdr Geothermal...

  12. Mi GmbH | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Mi GmbH Jump to: navigation, search Name: Mi GmbH Place: Switzerland Zip: CH-6340 Sector: Solar Product: Baar-based manufacturer and distributor of fruit juices. The firm is also...

  13. CH2M HILL Plateau Remediation Company | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    CH2M HILL Plateau Remediation Company CH2M HILL Plateau Remediation Company The Office of Hea1th, Safety and Security's Office of Enforcement and Oversight has evaluated the facts and circumstances of a series of radiological work deficiencies at the Plutonium Finishing Plant (PFP) and the 105 K-East Reactor Facility (105KE Reactor) by CH2M HILL Plateau Remediation Company (CHPRC). The radiological work deficiencies at PFP are documented in the April 29, 2011, Department of Energy Richland

  14. SECRETARY MONIZ TO HOST NATIONAL LABORATORY DAY ON CAPITOL HILL |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy SECRETARY MONIZ TO HOST NATIONAL LABORATORY DAY ON CAPITOL HILL SECRETARY MONIZ TO HOST NATIONAL LABORATORY DAY ON CAPITOL HILL September 12, 2014 - 10:16am Addthis News Media Contact 202 - 586 - 4940 Washington D.C. - On Tuesday, Secretary of Energy Ernest Moniz will join Senator Dick Durbin and Senator Jim Risch for the Department's National Lab Day on the Hill. Lab Directors and representatives from all 17 of the Department of Energy National Laboratories will be on

  15. Hills Creek-Lookout Point Transmission Line Rebuild Project

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    In c o o per a t io n wi t h t h e U. S . F or es t Ser v ic e Hills Creek-Lookout Point Transmission Line Rebuild Project Draft Environmental Assessment August 2016 DOE/EA-1967 Hills Creek-Lookout Point Transmission Line Rebuild Project Draft Environmental Assessment Bonneville Power Administration In c o o per a t io n wi t h t h e U. S . F or es t Ser v ic e August 2016 Table of Contents Hills Creek-Lookout Point Transmission Line Rebuild Project Draft Environmental Assessment i Table of

  16. miRNAs in brain development

    SciTech Connect

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan

    2014-02-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.

  17. Inglewood-Finn Hill, Washington: Energy Resources | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    can help OpenEI by expanding it. Inglewood-Finn Hill is a census-designated place in King County, Washington.1 References US Census Bureau 2005 Place to 2006 CBSA...

  18. East Hill-Meridian, Washington: Energy Resources | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    You can help OpenEI by expanding it. East Hill-Meridian is a census-designated place in King County, Washington.1 References US Census Bureau 2005 Place to 2006 CBSA...

  19. Clyde Hill, Washington: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    This article is a stub. You can help OpenEI by expanding it. Clyde Hill is a city in King County, Washington. It falls under Washington's 8th congressional district.12...

  20. Lea Hill, Washington: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    a stub. You can help OpenEI by expanding it. Lea Hill is a census-designated place in King County, Washington.1 References US Census Bureau 2005 Place to 2006 CBSA...

  1. Loess Hills Wind Energy LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    LLC Jump to: navigation, search Name: Loess Hills Wind Energy LLC Address: PO Box 198 Place: Malvern, Iowa Zip: 51551 Sector: Wind energy Product: ReDriven Wind Energy Systems...

  2. Signal Hill, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Signal Hill is a city in Los Angeles County, California. It falls under California's 37th...

  3. Spring Hill, Kansas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    This article is a stub. You can help OpenEI by expanding it. Spring Hill is a city in Johnson County and Miami County, Kansas. It falls under Kansas's 3rd congressional district...

  4. Mission Hills, Kansas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    article is a stub. You can help OpenEI by expanding it. Mission Hills is a city in Johnson County, Kansas. It falls under Kansas's 3rd congressional district.12 References...

  5. Westwood Hills, Kansas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    article is a stub. You can help OpenEI by expanding it. Westwood Hills is a city in Johnson County, Kansas. It falls under Kansas's 3rd congressional district.12 References...

  6. Bunker Hill Village, Texas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    is a stub. You can help OpenEI by expanding it. Bunker Hill Village is a city in Harris County, Texas. It falls under Texas's 7th congressional district.12 References ...

  7. Harris Hill, New York: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Harris Hill is a census-designated place in Erie County, New York.1 References US...

  8. Emerald Lake Hills, California: Energy Resources | Open Energy...

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Emerald Lake Hills is a census-designated place in San Mateo County, California.1...

  9. HDR Geothermal Energy: Important Lessons From Fenton Hill

    National Nuclear Security Administration (NNSA)

    Stanford University, Stanford, California, February 9-11, 2009 SGP-TR-187 HOT DRY ROCK GEOTHERMAL ENERGY: IMPORTANT LESSONS FROM FENTON HILL Donald W. Brown Los Alamos National...

  10. Town of Chapel Hill - Worthwhile Investments Save Energy (WISE...

    Energy.gov [DOE] (indexed site)

    Rebate Amount 20% - 40% of cost, depending on equipment or repair types. Summary Chapel Hill is using money made available to it from the American Recovery and Reinvestment Act of...

  11. Hill County, Texas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Hill County is a county in Texas. Its FIPS County Code is 217. It is classified as ASHRAE...

  12. Hill City, Minnesota: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Hill City is a city in Aitkin County, Minnesota. It falls under Minnesota's 8th...

  13. Pleasant Hill, California: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Pleasant Hill is a city in Contra Costa County, California. It falls under California's 10th...

  14. Rocky Hill, New Jersey: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Rocky Hill is a borough in Somerset County, New Jersey. It falls under New Jersey's 7th...

  15. Pleasant Hill, Iowa: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Pleasant Hill is a city in Polk County, Iowa. It falls under Iowa's 3rd congressional district.12...

  16. Rocky Hill, Connecticut: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Rocky Hill, Connecticut: Energy Resources Jump to: navigation, search This article is a stub. You can help OpenEI by expanding it. Equivalent URI DBpedia Coordinates 41.6648216,...

  17. Pleasant Hill, Ohio: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Pleasant Hill is a village in Miami County, Ohio. It falls under Ohio's 8th congressional...

  18. Blue Hills, Connecticut: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Blue Hills is a census-designated place in Hartford County, Connecticut.1 References ...

  19. Three Principal Results from Recent Fenton Hill Flow Testing...

    OpenEI (Open Energy Information) [EERE & EIA]

    future HDR development than the two-well system tested at Fenton Hill. Authors Donald Brown and Robert DuTeaux Conference 21st Workshop on Geothermal Reservoir Engineering;...

  20. Meridian Hills, Indiana: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    article is a stub. You can help OpenEI by expanding it. Meridian Hills is a town in Marion County, Indiana. It falls under Indiana's 5th congressional district and Indiana's 7th...

  1. Spring Hill, Indiana: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    This article is a stub. You can help OpenEI by expanding it. Spring Hill is a town in Marion County, Indiana. It falls under Indiana's 7th congressional district.12 References...

  2. West Lake Hills, Texas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. West Lake Hills is a city in Travis County, Texas. It falls under Texas's 10th congressional...

  3. West Hills, New York: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. West Hills is a census-designated place in Suffolk County, New York.1 References US...

  4. Red Hill, Pennsylvania: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Red Hill is a borough in Montgomery County, Pennsylvania. It falls under Pennsylvania's...

  5. Society Hill, New Jersey: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Society Hill is a census-designated place in Middlesex County, New Jersey.1 References ...

  6. CH2M Hill Ltd | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    in consulting, design, engineering, procurement, construction, and operations and maintenance. References: CH2M Hill Ltd1 This article is a stub. You can help OpenEI by...

  7. Science on the Hill: Why space weather matters

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Why space weather matters Science on the Hill: Why space weather matters Many people think of space as a silent, empty void and the sun as a distant source of light and heat. Not ...

  8. Voluntary Protection Program Onsite Review, CH2M HILL Analytical...

    Energy.gov [DOE] (indexed site)

    Evaluation to determine whether CH2M HILL Analytical Technical Services is continuing to perform at a level deserving DOE-VPP Star recognition. The Team conducted its review during...

  9. Aspen Hill, Maryland: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    You can help OpenEI by expanding it. Aspen Hill is a census-designated place in Montgomery County, Maryland.1 References US Census Bureau 2005 Place to 2006 CBSA...

  10. Gold Hill, Colorado: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Gold Hill is a census-designated place in Boulder County, Colorado.1 References US...

  11. Gold Hill, Oregon: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Gold Hill is a city in Jackson County, Oregon. It falls under Oregon's 2nd congressional...

  12. Black Hills Energy (Gas) - Residential Energy Efficiency Program...

    Energy.gov [DOE] (indexed site)

    70% of cost Duct Repair and Sealing: 70% of cost Doors: 25 Summary Black Hills Energy (BHE) offers a variety of rebates for residential Colorado customers who purchase and...

  13. Town of Kill Devil Hills- Wind Energy Systems Ordinance

    Energy.gov [DOE]

    In October 2007, the town of Kill Devil Hills adopted an ordinance to regulate the use of wind-energy systems. The ordinance directs any individual or organization wishing to install a wind-energy...

  14. Rock Hill Utilities- Water Heater and Heat Pump Rebate Program

    Energy.gov [DOE]

    Through the SmartChoice program, Rock Hill Utilities offers rebates for water heater and heat pump replacements. Information on financing for heat pumps can also be found on the web site listed...

  15. Town of Chapel Hill- Land-Use Management Ordinance

    Energy.gov [DOE]

    In 2003, the Town of Chapel Hill adopted a land-use management ordinance that includes prohibitions against neighborhood or homeowners association covenants or other conditions of sale that...

  16. Flint Hills Rural E C A, Inc | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Rural E C A, Inc Jump to: navigation, search Name: Flint Hills Rural E C A, Inc Place: Kansas Phone Number: 620-767-5144 Website: www.flinthillsrec.com Facebook: https:...

  17. Science on the Hill: Turning windows into solar panels

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science on the Hill: Turning windows into solar panels Turning windows into solar panels Working with quantum dots, researchers achieve a breakthrough in solar-concentrating technology that can turn windows into electric generators. February 7, 2016 solar panel windows The luminescent solar concentrator could turn any window into a daytime power source. Science on the Hill: Turning windows into solar panels Sunlight is abundant, free and for all practical purposes, eternal. Harvesting that light

  18. An implementation of Hill`s theory of normal anisotropic plasticity for explicit shell analysis

    SciTech Connect

    Whirley, R.G.; Engelmann, B.E.

    1991-08-20

    This paper summarizes the formulation and numerical implementation of a general anisotropic elastic-plastic material model for shell analysis. The 1948 Hill yield function is presented and specialized to conditions of plane stress. Next, an unconditionally stable and fully vectorized numerical algorithm for this constitutive model is presented. Finally, the model is specialized to conditions of normal anisotropy, and the implementation in DYNA3D is discussed. This development in material modeling should substantially extend the applicability of DYNA3D for many sheet metal forming applications. Several large-scale sheet metal forming examples are presented to illustrate these new analysis capabilities. 9 refs.

  19. EA-1824: Record Hill Wind Project in Roxbury, ME | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    4: Record Hill Wind Project in Roxbury, ME EA-1824: Record Hill Wind Project in Roxbury, ME July 1, 2011 EA-1824: Final Environmental Assessment Loan Guarantee to Record Hill Wind, LLC for Construction of a Wind Energy Project in Roxbury, Maine July 11, 2011 EA-1824: Finding of No Significant Impact Loan Guarantee to Record Hill Wind, LLC, for the Record Hill Wind Project, Maine

  20. Communities Plug In To Electric Vehicle Readiness | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Communities Plug In To Electric Vehicle Readiness Communities Plug In To Electric Vehicle Readiness September 16, 2014 - 4:24pm Addthis The City of Auburn Hills, Michigan, participated in one of 16 Clean Cities plug-in electric vehicle (PEV) community-readiness projects across the country. | Photo courtesy of City of Auburn Hills. The City of Auburn Hills, Michigan, participated in one of 16 Clean Cities plug-in electric vehicle (PEV) community-readiness projects across the country. | Photo

  1. Guardian Industries Corp | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Industries Corp Jump to: navigation, search Name: Guardian Industries Corp Place: Auburn Hills, Michigan Zip: 48326-1714 Sector: Solar Product: Michigan-based firm that...

  2. Workplace Charging Challenge Partner: FEV North America Inc....

    Energy Saver

    At the company's Auburn Hills, Michigan campus, FEV North America, Inc. has installed several charging stations that are available to employees with plug-in electric vehicles. This ...

  3. 2013 strategic petroleum reserve big hill well integrity grading report.

    SciTech Connect

    Lord, David L.; Roberts, Barry L.; Lord, Anna C. Snider; Bettin, Giorgia; Sobolik, Steven Ronald; Park, Byoung Yoon; Rudeen, David Keith; Eldredge, Lisa; Wynn, Karen; Checkai, Dean; Perry, James Thomas

    2014-02-01

    This report summarizes the work performed in developing a framework for the prioritization of cavern access wells for remediation and monitoring at the Big Hill Strategic Petroleum Reserve site. This framework was then applied to all 28 wells at the Big Hill site with each well receiving a grade for remediation and monitoring. Numerous factors affecting well integrity were incorporated into the grading framework including casing survey results, cavern pressure history, results from geomechanical simulations, and site geologic factors. The framework was developed in a way as to be applicable to all four of the Strategic Petroleum Reserve sites.

  4. Economic Development Benefits of the Mars Hill Wind Farm, Wind Powering America Rural Economic Development, Case Study (Fact Sheet)

    SciTech Connect

    Not Available

    2009-06-01

    This case study summarizes the economic development benefits of the Mars Hill Wind Farm to the community of Mars Hill, Maine. The Mars Hill Wind Farm is New England's first utility-scale wind farm.

  5. DOE - Office of Legacy Management -- Detrex Corp - MI 10

    Office of Legacy Management (LM)

    Detroit , Michigan MI.10-1 Evaluation Year: 1987 MI.10-2 Site Operations: Conducted experimental runs relative to picklingdegreasing of one handful of uranium turnings MI.10-1...

  6. DOE - Office of Legacy Management -- Adrian - MI 01

    Office of Legacy Management (LM)

    Adrian - MI 01 FUSRAP Considered Sites Adrian, MI Alternate Name(s): Bridgeport Brass Co. Special Metals Extrusion Plant Bridgeport Brass Company General Motors General Motors Company, Adrian MI.01-1 Location: 1450 East Beecher Street, Adrian, Michigan MI.01-3 Historical Operations: Performed uranium extrusion research and development and metal fabrication work for the AEC using uranium, thorium, and plutonium. MI.01-2 Eligibility Determination: Eligible MI.01-1 Radiological Survey(s):

  7. Painted Hills B&C Wind Farm II | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    II Jump to: navigation, search Name Painted Hills B&C Wind Farm II Facility Painted Hills B&C Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  8. Ben Hill County, Georgia ASHRAE 169-2006 Climate Zone | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    Ben Hill County, Georgia ASHRAE 169-2006 Climate Zone Jump to: navigation, search County Climate Zone Place Ben Hill County, Georgia ASHRAE Standard ASHRAE 169-2006 Climate Zone...

  9. DOE Selects CH2M Hill Plateau Remediation Company for Plateau...

    Office of Environmental Management (EM)

    CH2M Hill Plateau Remediation Company for Plateau Remediation Contract at its Hanford Site DOE Selects CH2M Hill Plateau Remediation Company for Plateau Remediation Contract at its ...

  10. Enforcement Letter, CH2M Hill Hanford Group, Inc - July 8, 2005...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Inc - July 8, 2005 Enforcement Letter, CH2M Hill Hanford Group, Inc - July 8, 2005 July 8, 2005 Issued to CH2M Hill Hanford Group, Inc., related to Neutron Exposure at the Hanford...

  11. Science on the Hill: Portable MRI might make the world a better...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Portable MRI might make the world a better placer Science on the Hill: Portable MRI might ... can be used in battlefield hospitals. Science on the Hill: Portable MRI might make the ...

  12. Science on the Hill: Rapid diagnosis a new weapon against re...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Rapid diagnosis a new weapon against re-emerging TB Science on the Hill: Rapid diagnosis a ... Los Alamos biomedical scientist Harshini Mukundan Science on the Hill: Rapid diagnosis a ...

  13. Flint Hills Resources, LP- FE Dkt. No. 15-168-LNG

    Energy.gov [DOE]

    The Office of Fossil Energy (FE) of the Department of Energy (DOE) gives notice of receipt of an application, filed on November 5, 2015, by Flint Hills Resources, LP (Flint Hills), requesting long...

  14. Geology of Geothermal Test Hole GT-2 Fenton Hill Site, July 1974...

    OpenEI (Open Energy Information) [EERE & EIA]

    Geothermal Test Hole GT-2 Fenton Hill Site, July 1974 Jump to: navigation, search OpenEI Reference LibraryAdd to library Report: Geology of Geothermal Test Hole GT-2 Fenton Hill...

  15. Town of Chapel Hill- Worthwhile Investments Save Energy (WISE) Homes and Buildings Program

    Energy.gov [DOE]

    Chapel Hill is using money made available to it from the American Recovery and Reinvestment Act of 2009 to help subsidize energy efficiency improvements in Chapel Hill homes. Qualified homeowners...

  16. Preliminary Notice of Violation, CH2M Hill Hanford Group, Inc...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Preliminary Notice of Violation, CH2M Hill Hanford Group, Inc. - EA-2006-06 November 16, 2006 Issued to CH2M Hill Hanford Group, Inc., related to Radiological Contamination Events ...

  17. DOE - Office of Legacy Management -- Oliver Corp - MI 11

    Office of Legacy Management (LM)

    OLIVER CORP. (MI.11 ) Eliminated from further consideration under FUSRAP - Referred to NRC Designated Name: Not Designated Alternate Name: Behnke Warehousing Incorporated MI.11-1...

  18. Preliminary Notice of Violation, CH2M Hill Hanford Group, Inc.- EA-2003-06

    Energy.gov [DOE]

    Issued to CH2M Hill Hanford Group, Inc., related to Quality Assurance Issues at the Hanford Site Tank Farms

  19. Hill named a fellow of the American Nuclear Society | Argonne National

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Laboratory Watch Hill explain Argonne's research on nuclear energy and its importance. Nuclear Energy Technologies Robert Hill Hill named a fellow of the American Nuclear Society November 17, 2016 Tweet EmailPrint Bob Hill, technical director of advanced nuclear energy research and development at the U.S. Department of Energy's (DOE's) Argonne National Laboratory, was honored last week as a Fellow of the American Nuclear Society (ANS). Fellow is the highest member status presented by the

  20. Independent Activity Report, CH2M Hill Plateau Remediation Company- January 2011

    Energy.gov [DOE]

    Review of the CH2M Hill Plateau Remediation Company Unreviewed Safety Question Procedure [ARPT-RL-2011-003

  1. Enforcement Letter, CH2M Hill Hanford Group, Inc.- April 24, 2001

    Energy.gov [DOE]

    Issued to CH2M Hill Hanford Group, Inc., related to Nuclear Safety Management at the Hanford Site Tank Farms

  2. Preliminary Notice of Violation, CH2M Hill Hanford Group, Inc- EA-2005-01

    Energy.gov [DOE]

    Issued to CH2M Hill Hanford Group, Inc., related to Radiological and Operational Events at the Hanford Tank Farms

  3. Enforcement Letter, Kaiser-Hill Company, L.L.C.- June 19, 2002

    Office of Energy Efficiency and Renewable Energy (EERE)

    Issued to Kaiser-Hill Company, LLC related to Unplanned Radioactive Material Uptakes at the Rocky Flats Environmental Technology Site

  4. Enforcement Letter, CH2M Hill Hanford Group Inc,- September 6, 2007

    Energy.gov [DOE]

    Issued to CH2M Hill Hanford Group, Inc., related to Quality Improvement Deficiencies at the Hanford Tank Farms

  5. SummerHill Homes, San Francisco Bay Area, Fremont, California

    SciTech Connect

    2006-10-01

    Building America fact sheet on SummerHill Homes of Northern California. The Villa Savona Homes in Fremont, California were built using 15% fly ash in concrete, engineered lumber for floors, high efficiency windows with Low-emissivity (Low-E) glass, and fi

  6. Science of Safety in Spotlight on Capitol Hill

    Energy.gov [DOE]

    WASHINGTON, D.C. – Sporting camera lenses for eyes, four wheels for feet, a pipestem torso and gloves covering metallic “fingers,” a 650-pound “Bimanual Dexterous Robotic Platform” more commonly known as “Robo Sally” waved and shook hands with people entering the Capitol Hill conference room.

  7. The NuMI neutrino beam

    DOE PAGES [OSTI]

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; et al

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  8. The NuMI Neutrino Beam

    SciTech Connect

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; Barish, B.; Barr, G.; Barrett, W. L.; Berstein, R. H.; Biggs, J.; Bishai, M.; Blake, A.; Bocean, V.; Bock, G. J.; Boehnlein, D. J.; Bogert, D.; Bourkland, K.; Cao, S. V.; Castromonte, C. M.; Childress, S.; Choudhary, B. C.; Coelho, J. A.B.; Cobb, J. H.; Corwin, L.; Crane, D.; Cravens, J. P.; Cronin-Hennessy, D.; Ducar, R. J.; De Jong, J. K.; Devan, A. V.; Devenish, N. E.; Diwan, M. V.; Erwin, A. R.; Escobar, C. O.; Evans, J. J.; Falk, E.; Feldman, G. J.; Fields, T. H.; Ford, R.; Frohne, M. V.; Gallahger, H. R.; Garkusha, V.; Gomes, R. A.; Goodman, M. C.; Gouffon, P.; Graf, N.; Gran, R.; Grossman, N.; Grzelak, K.; Habig, A.; Hahn, S. R.; Harding, D.; Harris, D.; Harris, P. G.; Hartnell, J.; Hatcher, R.; Hays, S.; Heller, K.; Holin, A.; Huang, J.; Hylen, J.; Ibrahim, A.; Indurthy, D.; Irwin, G. M.; Isvan, Z.; Jaffe, D. E.; James, C.; Jensen, D.; Johnstone, J.; Kafka, T.; Kasahara, S. M.S.; Koizumi, G.; Kopp, S.; Kordosky, M.; Kreymer, A.; Lang, K.; Laughton, C.; Lefeuvre, G.; Ling, J.; Litchfield, P. J.; Loiacono, L.; Lucas, P.; Mann, W. A.; Marchionni, A.; Marshak, M. L.; Mayer, N.; McGivern, C.; Medeiros, M. M.; Mehdiyev, R.; Meier, J. R.; Messier, M. D.; Michael, D. G.; Milburn, R. H.; Miller, J. L.; Miller, W. H.; Mishra, S. R.; Sher, S. Moed; Moore, C. D.; Morfin, J.; Mualem, L.; Mufson, S.; Murgia, S.; Murtagh, M.; Musser, J.; Naples, D.; Nelson, J. K.; Newman, H. B.; Nichol, R. J.; Nowak, J. A.; O'connor, J.; Oliver, W. P.; Olsen, M.; Orchanian, M.; Osprey, S.; Pahlka, R. B.; Paley, J.; Para, A.; Patterson, R. B.; Patzak, T.; Pavlovic, Z.; Pawloski, G.; Perch, A.; Peterson, E. A.; Petyt, D. A.; Pfutzner, M. M.; Phan-Budd, S.; Plunkett, R. K.; Poonthottathil, N.; Prieto, P.; Pushka, D.; Qiu, X.; Radovic, A.; Rameika, R. A.; Ratchford, J.; Rebel, B.; Reilly, R.; Rosenfeld, C.; Rubin, H. A.; Ruddick, K.; Sanchez, M. C.; Saoulidou, N.; Sauer, L.; Schneps, J.; Schoo, D.; Schreckenberger, A.; Schreiner, P.; Shanahan, P.; Sharma, R.; Smart, W.; Smith, C.; Sousa, A.; Stefanik, A.; Tagg, N.; Talaga, R. L.; Tassotto, G.; Thomas, J.; Thompson, J.; Thomson, M. A.; Tian, X.; Timmons, A.; Tinsley, D.; Tognini, S. C.; Toner, R.; Torretta, D.; Trostin, I.; Tzanakos, G.; Urheim, J.; Vahle, P.; Vaziri, K.; Villegas, E.; Viren, B.; Vogel, G.; Webber, R. C.; Weber, A.; Webb, R. C.; Wehmann, A.; White, C.; Whitehead, L.; Whitehead, L. H.; Wojcicki, S. G.; Wong-Squires, M. L.; Yang, T.; Yumiceva, F. X.; Zarucheisky, V.; Zwaska, R.

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  9. Environmental protection and regulatory compliance at the Elk Hills Field

    SciTech Connect

    Chappelle, H.H.; Donahoe, R.L.; Kato, T.T.; Ordway, H.E.

    1991-12-31

    Environmental protection has played an integral role in the development and operation of the Elk Hills field since production at the maximum efficient rate was authorized in 1976. The field is located in a non-attainment area for California and National Ambient Air Quality Standards for two criteria pollutants and their associated precursors, is home to four endangered species, and operates within the California regulatory framework. Environmental protection and regulatory compliance is a multi-faceted program carried out through a substantial commitment of resources and workforce involvement. This paper describes the actions taken and resources employed to protect the environment, specific technologies and projects implement, and the ongoing nature of these efforts at Elk Hills.

  10. Environmental protection and regulatory compliance at the Elk Hills Field

    SciTech Connect

    Chappelle, H.H. ); Donahoe, R.L. ); Kato, T.T. ); Ordway, H.E. )

    1991-01-01

    Environmental protection has played an integral role in the development and operation of the Elk Hills field since production at the maximum efficient rate was authorized in 1976. The field is located in a non-attainment area for California and National Ambient Air Quality Standards for two criteria pollutants and their associated precursors, is home to four endangered species, and operates within the California regulatory framework. Environmental protection and regulatory compliance is a multi-faceted program carried out through a substantial commitment of resources and workforce involvement. This paper describes the actions taken and resources employed to protect the environment, specific technologies and projects implement, and the ongoing nature of these efforts at Elk Hills.

  11. Microsoft PowerPoint - 6 Mary Hill & Ming Ye

    Office of Environmental Management (EM)

    Analysis and Decision-Making Under Uncertainty: A Strategy and its Applications Ming Ye (mye@fsu.edu) Florida State University Mary Hill (mchill@ku.edu) University of Kansas 1 This research is supported by DOE Early Career Award: DE-SC0008272 Acknowledge the efforts of ISCMEM Working Group 2- Federal Scientists Working for Coordinated Uncertainty Analysis and Parameter Estimation Since 2002 Learning from the NAS workshop * Anna Willett, director of the Interstate Technology and Regulatory

  12. Three dimensional simulation for Big Hill Strategic Petroleum Reserve (SPR).

    SciTech Connect

    Ehgartner, Brian L.; Park, Byoung Yoon; Sobolik, Steven Ronald; Lee, Moo Yul

    2005-07-01

    3-D finite element analyses were performed to evaluate the structural integrity of caverns located at the Strategic Petroleum Reserve's Big Hill site. State-of-art analyses simulated the current site configuration and considered additional caverns. The addition of 5 caverns to account for a full site and a full dome containing 31 caverns were modeled. Operations including both normal and cavern workover pressures and cavern enlargement due to leaching were modeled to account for as many as 5 future oil drawdowns. Under the modeled conditions, caverns were placed very close to the edge of the salt dome. The web of salt separating the caverns and the web of salt between the caverns and edge of the salt dome were reduced due to leaching. The impacts on cavern stability, underground creep closure, surface subsidence and infrastructure, and well integrity were quantified. The analyses included recently derived damage criterion obtained from testing of Big Hill salt cores. The results show that from a structural view point, many additional caverns can be safely added to Big Hill.

  13. CX-002388: Categorical Exclusion Determination

    Energy.gov [DOE]

    Advancing Plug-In Hybrid Technology and Flex Fuel Application on a Chrysler Minivan - Auburn HillsCX(s) Applied: B3.6Date: 05/24/2010Location(s): Auburn Hills, MichiganOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  14. Auburn, Massachusetts: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Massachusetts: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 42.1945385, -71.8356271 Show Map Loading map... "minzoom":false,"mappingservice"...

  15. Auburn, Kansas: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Kansas: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 38.906114, -95.8160968 Show Map Loading map... "minzoom":false,"mappingservice":"google...

  16. First Secretarial Visit to the Big Hill SPR Site | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    First Secretarial Visit to the Big Hill SPR Site First Secretarial Visit to the Big Hill SPR Site October 16, 2014 - 2:45pm Addthis The Secretary was accompanied, during his visit, by (from left to right): Big Hill Site Director John Guidry, Field Operations Manager Darryl Rickner, Supervisory General Engineer Lionel Gele, Supervisory General Engineer Lisa Nicholson, Sr. Site Cavern Engineer Jim Perry, Daniel Evans, Office of Petroleum Reserves Deputy Assistant Secretary Robert Corbin, General

  17. EA-1967: Hills Creek-Lookout Point Transmission Line Rebuild Project; Lane

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    County, Oregon | Department of Energy 7: Hills Creek-Lookout Point Transmission Line Rebuild Project; Lane County, Oregon EA-1967: Hills Creek-Lookout Point Transmission Line Rebuild Project; Lane County, Oregon Summary Bonneville Power Administration is preparing an EA to assess potential environmental impacts of the proposed rebuild of its 26-mile 115-kilovolt (kV) wood-pole Hills Creek-Lookout Point transmission line, which is generally located between Lowell and Oakridge, in Lane County,

  18. Students from Smoky Hill High School Triumph in Colorado Science Bowl -

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    News Releases | NREL Students from Smoky Hill High School Triumph in Colorado Science Bowl February 8, 2003 Golden, CO. - Students from Smoky Hill High School won top honors at the Colorado Science Bowl today at the Colorado School of Mines. In the final round of rapid-fire questions and answers about physics, math, biology, astronomy, chemistry, computers and the earth sciences, students from Smoky Hill High School team #2 were victorious over their peers, Smoky Hill team #3. At the end of

  19. Students from Smoky Hill High School Triumph in Colorado Science Bowl -

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    News Releases | NREL Students from Smoky Hill High School Triumph in Colorado Science Bowl February 12, 2005 Golden, Colo. - Students from Smoky Hill High School won top honors at the Colorado Science Bowl today for the fourth year in a row. In the final round of rapid-fire questions and answers about physics, math, biology, astronomy, chemistry, computers and the earth sciences, Smoky Hill High School team #2 from Aurora was victorious over Smoky Hill High School team #1. Thirty-six student

  20. Preliminary Notice of Violation, Kaiser-Hill Company, LLC- EA-2000-01

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC, related to Recurring Procurement Quality Problems at the Rocky Flats Environmental Technology Site, January 24, 2000 (EA-2000-01)

  1. Enforcement Letter, CH2M Hill Mound, Inc- December 22, 2004

    Energy.gov [DOE]

    Issued to CH2M Hill Mound, Inc. related to a Radioactive Contamination Event during Remediation Activities at the Miamisburg Closure Project

  2. Preliminary Notice of Violation, Kaiser-Hill Company, LLC- EA-1999-06

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC, related to the Procurement of Waste Containers at the Rocky Flats Environmental Technology Site, August 18, 1999 (EA-1999-06

  3. Water Sampling At Fenton Hill HDR Geothermal Area (Rao, Et Al...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Water Sampling At Fenton Hill HDR Geothermal Area (Rao, Et Al., 1996) Exploration Activity...

  4. Water-Gas Sampling At Fenton Hill HDR Geothermal Area (Janik...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Water-Gas Sampling At Fenton Hill HDR Geothermal Area (Janik & Goff, 2002) Exploration...

  5. Enforcement Letter, Kaiser-Hill Company, L.L.C- September 11, 2000

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC related to Noncompliances with Design and Procurement Requirements at the Rocky Flats Environmental Technology Site

  6. Enforcement Letter, Kaiser-Hill Company, L.L.C.- July 20, 1998

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC related to Recurring Weaknesses in Implementing Quality Assurance Rule Requirements at the Rocky Flats Environmental Technology Site

  7. Constraints on the Age of Heating at the Fenton Hill Site, Valles...

    OpenEI (Open Energy Information) [EERE & EIA]

    close to Fenton Hill during the last few tens of thousands of years. Authors T. Mark Harrison, Paul Morgan and David D. Blackwell Published Journal Journal of Geophysical...

  8. Preliminary Notice of Violation, CH2M HILL Hanford Group, Inc.- NEA-2008-02

    Energy.gov [DOE]

    Issued to CH2M Hill Hanford Group, Inc., related to a Radioactive Waste Spill at the Hanford Site Tank Farms

  9. Preliminary Notice of Violation, Kaiser-Hill Company- EA-97-03

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company related to a Radioactive Material Release during Trench Remediation at the Rocky Flats Environmental Technology Site, (EA-97-03)

  10. Enforcement Letter, Kaiser-Hill Company, L.L.C.- July 21, 1998

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC related to Fire Protection System Surveillances at the Rocky Flats Environmental Technology Site, July 21, 1998

  11. Graduating to a Bright Tomorrow: Solar Ready Vets Class Graduates from Hill

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    AFB | Department of Energy Graduating to a Bright Tomorrow: Solar Ready Vets Class Graduates from Hill AFB Graduating to a Bright Tomorrow: Solar Ready Vets Class Graduates from Hill AFB March 30, 2016 - 11:49am Addthis Deputy Secretary Liz Sherwood-Randall with the graduates of the Solar Ready Vets program at Hill Air Force Base, Utah. Image courtesy of the U.S. Air Force. Deputy Secretary Liz Sherwood-Randall with the graduates of the Solar Ready Vets program at Hill Air Force Base, Utah.

  12. DOE Cites CH2M Hill Hanford for Violating Nuclear Safety Rules | Department

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of Energy for Violating Nuclear Safety Rules DOE Cites CH2M Hill Hanford for Violating Nuclear Safety Rules March 10, 2005 - 10:44am Addthis Hanford Tank Farm Contractor Faces Fine of more than $300,000 WASHINGTON, DC - The Department of Energy (DOE) today notified the CH2M Hill Hanford Group, Inc. (CH2M Hill) - that it will fine the company $316,250 for violations of the department's nuclear safety requirements. CH2M Hill is the department's contractor responsible for storage of highly

  13. Consent Order, Kaiser-Hill Company, LLC- EA 98-03

    Energy.gov [DOE]

    Issued to Kaiser-Hill Company, LLC related to three Radiological Events at the Rocky Flats Environmental Technology Site, (EA 98-03)

  14. Voluntary Protection Program Onsite Review, CH2M HILL Analytical Technical Services- January 2008

    Office of Energy Efficiency and Renewable Energy (EERE)

    Evaluation to determine whether CH2M HILL Analytical Technical Services is continuing to perform at a level deserving DOE-VPP Star recognition.

  15. Preliminary Notice of Violation, Kaiser-Hill Company, LLC- EA-2001-04

    Office of Energy Efficiency and Renewable Energy (EERE)

    Preliminary Notice of Violation issued to Kaiser-Hill Company, LLC, related to Nuclear Safety, Work Control, and Radiation Protection Deficiencies at the Rocky Flats Environmental Technology Site

  16. Flow Test At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) Exploration Activity Details...

  17. Flow Test At Fenton Hill HDR Geothermal Area (Grigsby, Et Al...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Grigsby, Et Al., 1983) Exploration Activity Details...

  18. Flow Test At Fenton Hill HDR Geothermal Area (Dash, 1989) | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Dash, 1989) Exploration Activity Details Location...

  19. AMO’s Industrial Assessment Center Program to be Recognized at Capitol Hill Event

    Energy.gov [DOE]

    Today the Industrial Assessment Center (IAC) program, which is sponsored by the Advanced Manufacturing Office (AMO), will be recognized on Capitol Hill. This recognition follows a Senate resolution...

  20. Consent Order, CH2M Hill Hanford Group, Inc.- EA-2000-09

    Energy.gov [DOE]

    Issued to CH2M Hill Hanford Group, Inc., related to Quality Problems at the Hanford Site Tank Farms, (EA-2000-09)

  1. AmeriFlux US-LPH Little Prospect Hill

    SciTech Connect

    Hadley, Julian

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-LPH Little Prospect Hill. Site Description - The site was cleared for pasture, but not deeply plowed or planted, in the 18th and 19th centuries. Agriculture on the site was abandoned near the end of the 19th century. The forest within 200 to 300 m of the eddy covariance tower to the NW, W, SW, and S burned in an intense fire in 1957, which left few or no surviving trees.

  2. Science on the Hill: Why space weather matters

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Why space weather matters Why space weather matters Many people think of space as a silent, empty void and the sun as a distant source of light and heat. Not true. The sun and the Earth are connected in complex, intimate and sometimes dangerous ways. April 10, 2016 Science on the Hill: Why space weather matters For years, space scientists thought the empty region between the two Van Allen belts filled in only during only the most extreme, once-a-decade geomagnetic storms. New research shows that

  3. Port Huron, MI Liquefied Natural Gas Exports to Canada (Million...

    Energy Information Administration (EIA) (indexed site)

    Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul ...

  4. DOE - Office of Legacy Management -- Naval Ordnance Plant - MI...

    Office of Legacy Management (LM)

    Eliminated from further consideration under FUSRAP - Referred to DoD for action Designated ... MI.0-03-1 Site Disposition: Eliminated - No Authority - Referred to DoD MI.0-03-1 ...

  5. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    SciTech Connect

    Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R.; Postier, Russell G.; Brackett, Daniel J.; Schmittgen, Thomas D.

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  6. “Nodal Gap” induced by the incommensurate diagonal spin density modulation in underdoped high- <mi>Tmi>c> superconductors

    SciTech Connect

    Zhou, Tao; Gao, Yi; Zhu, Jian -Xin

    2015-03-07

    Recently it was revealed that the whole Fermi surface is fully gapped for several families of underdoped cuprates. The existence of the finite energy gap along the <mi>d>-wave nodal lines (nodal gap) contrasts the common understanding of the <mi>d>-wave pairing symmetry, which challenges the present theories for the high-<mi>Tmi><mi>c>superconductors. Here we propose that the incommensurate diagonal spin-density-wave order can account for the above experimental observation. The Fermi surface and the local density of states are also studied. Our results are in good agreement with many important experiments in high-<mi>Tmi><mi>c>superconductors.

  7. Characterization of function and regulation of miR-24-1 and miR-31

    SciTech Connect

    Sun Fenyong; Wang Jiayi; Pan Qiuhui; Yu Yongchun; Zhang Yue; Wan Yang; Wang Ju; Li Xiaoyan; Hong An

    2009-03-13

    To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b{approx}27b{approx}24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.

  8. Independent Oversight Review, Richland Operations Office and CH2M Hill Plateau Remediation Company and Mission Support Alliance- April 2012

    Energy.gov [DOE]

    Review of Richland Operations Office and CH2M Hill Plateau Remediation Company and Mission Support Alliance Conduct of Operations

  9. Students from Smoky Hill High School Triumph in Colorado High School

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science Bowl - News Releases | NREL Students from Smoky Hill High School Triumph in Colorado High School Science Bowl February 11, 2006 Golden, Colo. - For the fifth year in a row, students from Smoky Hill High School won top honors at the Colorado High School Science Bowl. In the final round of rapid-fire questions and answers about physics, math, biology, astronomy, chemistry, computers and the earth sciences, Smoky Hill High School from Aurora was victorious over D'Evelyn High School from

  10. Students from Smoky Hill High School Triumph in Colorado Science Bowl -

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    News Releases | NREL Students from Smoky Hill High School Triumph in Colorado Science Bowl February 7, 2004 Golden, Colo. - Students from Smoky Hill High School won top honors today at the Colorado Science Bowl for the third year in a row. In the final round of rapid-fire questions and answers about physics, math, biology, astronomy, chemistry, computers and the earth sciences, Smoky Hill High School was victorious over Pueblo Centennial High School. Thirty-nine student teams from across the

  11. DOE Selects CH2M Hill Plateau Remediation Company for Plateau Remediation

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Contract at its Hanford Site | Department of Energy CH2M Hill Plateau Remediation Company for Plateau Remediation Contract at its Hanford Site DOE Selects CH2M Hill Plateau Remediation Company for Plateau Remediation Contract at its Hanford Site June 19, 2008 - 1:29pm Addthis WASHINGTON, DC - The U.S. Department of Energy (DOE) today announced that CH2M Hill Plateau Remediation Company has been selected as the plateau remediation contractor for DOE's Hanford Site in southeastern Washington

  12. Secretary Moniz's Remarks at National Lab Day on the Hill -- As Delivered |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy National Lab Day on the Hill -- As Delivered Secretary Moniz's Remarks at National Lab Day on the Hill -- As Delivered September 16, 2014 - 5:08pm Addthis Dr. Ernest Moniz Dr. Ernest Moniz Secretary of Energy Welcome to everyone here. It's just - it was remarkable to walk in here to this jammed and very kind of buzzing room with our first Lab Day on the Hill. I see at least a couple of our lab directors - oh, I see more of our lab directors here. That's right. That's

  13. SEP Success Story: Turkey Hill Dairy: Where Energy is Not Left Flapping in

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    the Wind | Department of Energy Turkey Hill Dairy: Where Energy is Not Left Flapping in the Wind SEP Success Story: Turkey Hill Dairy: Where Energy is Not Left Flapping in the Wind December 21, 2011 - 11:57am Addthis These two General Electric wind turbines, erected in January 2011 on the Frey Farm landfill adjacent to Turkey Hill Dairy's ice cream and sweet iced tea plant in Lancaster County, Penn., are expected to produce 7.5 million kWh of electricity annually. | Photo courtesy of

  14. NORTH HILL CREEK 3-D SEISMIC EXPLORATION PROJECT

    SciTech Connect

    Marc T. Eckels; David H. Suek; Denise H. Harrison; Paul J. Harrison

    2004-05-06

    Wind River Resources Corporation (WRRC) received a DOE grant in support of its proposal to acquire, process and interpret fifteen square miles of high-quality 3-D seismic data on non-allotted trust lands of the Uintah and Ouray (Ute) Indian Reservation, northeastern Utah, in 2000. Subsequent to receiving notice that its proposal would be funded, WRRC was able to add ten square miles of adjacent state and federal mineral acreage underlying tribal surface lands by arrangement with the operator of the Flat Rock Field. The twenty-five square mile 3-D seismic survey was conducted during the fall of 2000. The data were processed through the winter of 2000-2001, and initial interpretation took place during the spring of 2001. The initial interpretation identified multiple attractive drilling prospects, two of which were staked and permitted during the summer of 2001. The two initial wells were drilled in September and October of 2001. A deeper test was drilled in June of 2002. Subsequently a ten-well deep drilling evaluation program was conducted from October of 2002 through March 2004. The present report discusses the background of the project; design and execution of the 3-D seismic survey; processing and interpretation of the data; and drilling, completion and production results of a sample of the wells drilled on the basis of the interpreted survey. Fifteen wells have been drilled to test targets identified on the North Hill Creek 3-D Seismic Survey. None of these wildcat exploratory wells has been a dry hole, and several are among the best gas producers in Utah. The quality of the data produced by this first significant exploratory 3-D survey in the Uinta Basin has encouraged other operators to employ this technology. At least two additional 3-D seismic surveys have been completed in the vicinity of the North Hill Creek Survey, and five additional surveys are being planned for the 2004 field season. This project was successful in finding commercial oil, natural gas

  15. Magnetocrystalline anisotropy in <mi>UMn>2<mi>Ge>2 and related Mn-based actinide ferromagnets

    SciTech Connect

    Parker, David S.; Ghimire, Nirmal; Singleton, John; Thompson, J. D.; Bauer, Eric D.; Baumbach, Ryan; Mandrus, David; Li, Ling; Singh, David J.

    2015-05-04

    We present magnetization isotherms in pulsed magnetic fields up to 62 Tesla, supported by first principles calculations, demonstrating a huge uniaxial magnetocrystalline anisotropy energy - approximately 20 MJ/m3 - in <mi>UMn>2<mi>Ge>2. This large anisotropy results from the extremely strong spin-orbit coupling affecting the uranium 5 f electrons, which in the calculations exhibit a substantial orbital moment exceeding 2 μB. Finally, we also find from theoretical calculations that a number of isostructural Mn-actinide compounds are expected to have similarly large anisotropy.

  16. DOE - Office of Legacy Management -- Star Cutter Corp - MI 15

    Office of Legacy Management (LM)

    Eliminated - Potential for contamination considered remote based on limited scope and quantity of materials handled MI.15-2 Radioactive Materials Handled: Yes Primary ...

  17. MINOS Experiment and NuMI Beam Home Page

    U.S. Department of Energy (DOE) - all webpages

    NuMI-MINOS Neutrino Logo NuMI Beamline and MINOS Experiment Neutrino Logo The MINOS Experiment and NuMI Beamline Fermilab Logo MINOS Experiment Links ◊ MINOS for the Public ◊ Scientific Results ◊ MINOS at Work ◊ NuMI at Work ◊ MINOS+ Experiment Fermilab Neutrino Links ◊ Neutrino FAQ ◊ MINOS Underground Areas at Fermilab ◊ PPD Intensity Frontier Dept Back to - - - ◊ Fermilab at Work ◊ Fermilab Home the MINOS Far Detector in the Soudan Mine MINOS collaborators assembling the

  18. 2575 Sand Hill Road * Mail Stop 103 * Menlo Park, CA 94025...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    575 Sand Hill Road * Mail Stop 103 * Menlo Park, CA 94025-7015 650-926-2288 * Fax 650-926-4695 SLAC is operated by Stanford University for the U.S. Department of Energy SLAC ...

  19. U.S. strategic petroleum reserve Big Hill 114 leak analysis 2012...

    Office of Scientific and Technical Information (OSTI)

    U.S. strategic petroleum reserve Big Hill 114 leak analysis 2012. Citation Details ... DM has since isolated the leak in well A with a temporary plug, and is planning on ...

  20. Voluntary Protection Program Onsite Review, CH2M HILL B&W West...

    Office of Environmental Management (EM)

    B&W West Valley LLC, West Valley Demonstration Project - October 2013 Voluntary Protection Program Onsite Review, CH2M HILL B&W West Valley LLC, West Valley Demonstration Project - ...

  1. SEP Success Story: Turkey Hill Dairy: Where Energy is Not Left...

    Energy Saver

    Turkey Hill Dairy, the fourth largest producer of ice cream in the United States, uses wind power to produce its 26 million gallons of ice cream each year. Learn more. Addthis ...

  2. Farmington Hills Partners With Michigan Saves With Eyes on the Energy Prize

    Energy.gov [DOE]

    Better Buildings Residential Network member Farmington Hills, Michigan, held a January kick-off event to announce its plans to launch a host of programs designed to reduce residential and municipal...

  3. Rock-Water Interactions in the Fenton Hill, New Mexico, Hot Dry...

    OpenEI (Open Energy Information) [EERE & EIA]

    to: navigation, search OpenEI Reference LibraryAdd to library Journal Article: Rock-Water Interactions in the Fenton Hill, New Mexico, Hot Dry Rock Geothermal Systems I. Fluid...

  4. DOE Tour of Zero: The Candlewood Hills Rebuild by BPC Green Builders...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    DOE Tour of Zero: The Candlewood Hills Rebuild by BPC Green Builders Addthis 1 of 27 BPC Green Builders built this certified U.S. DOE Zero Energy Ready Home overlooking Candlewood ...

  5. Consent Order between Department of Energy and Kaiser-Hill Company...

    Office of Environmental Management (EM)

    ... a Final Order upon receipt of said amount referenced in Item 2. 5. Kaiser-Hill hereby waives any and all rights to appeal or otherwise seek judicial review of this Consent Order. ...

  6. Preliminary Notice of Violation, Kaiser-Hill Company, LLC- EA-96-04

    Energy.gov [DOE]

    Preliminary Notice of Violation issued to Kaiser-Hill Company, LLC related to Radiological and Work Control Deficiencies associated with Two Radiological Release Events at the Rocky Flats Environmental Technology Site, (EA-96-04)

  7. Preliminary Notice of Violation, Kaiser-Hill Company, LLC- EA-2000-05

    Energy.gov [DOE]

    Preliminary Notice of Violation issued to Kaiser-Hill Company, LLC, related to an Unplanned, Radioactive Material Uptake at the Rocky Flats Environmental Technology Site, (EA-2000-05)

  8. Los Alamos National Laboratory Investigates Fenton Hill to Support Future Land Use

    Energy.gov [DOE]

    LOS ALAMOS, N.M. – Supporting future land use for the U.S. Forest Service, Los Alamos National Laboratory’s Corrective Actions Program (CAP) completed sampling soil at Fenton Hill in the Jemez Mountains this month.

  9. EIS-0266: Glass Mountain/Four Mile Hill Geothermal Project, California

    Office of Energy Efficiency and Renewable Energy (EERE)

    The EIS analyzes BPA's proposed action to approve the Transmission Services Agreements (TSAs) and Power Purchase Agreements (PPAs) with Calpine Siskiyou Geothermal Partners, L.P. (Calpine) to acquire output from the Fourmile Hill Geothermal Development Project (Project).

  10. DOE Cites CH2M Hill Hanford Group for Price-Anderson Violations

    Energy.gov [DOE]

    WASHINGTON, DC - The Department of Energy (DOE) today notified CH2M Hill Hanford Group (CHG) that it will fine the company $82,500 for violations of the Department's nuclear safety requirements. ...

  11. DOE Cites CH2M Hill Hanford Group, Inc. for Price-Anderson Violations

    Energy.gov [DOE]

    WASHINGTON, DC - The Department of Energy (DOE) today issued a Preliminary Notice of Violation (PNOV) to CH2M Hill Hanford Group, Inc. (CHG) for nuclear safety violations.  CHG is the tank...

  12. ICFT- An Initial Closed-Loop Flow Test of the Fenton Hill Phase...

    OpenEI (Open Energy Information) [EERE & EIA]

    ICFT- An Initial Closed-Loop Flow Test of the Fenton Hill Phase II HDR Reservoir Jump to: navigation, search OpenEI Reference LibraryAdd to library Report: ICFT- An Initial...

  13. AMO Celebrates 40 Years of Industrial Assessment Center Program at Capitol Hill Event

    Energy.gov [DOE]

    Last week the Advanced Manufacturing Office’s (AMO) Industrial Assessment Center (IAC) program was recognized during a bipartisan briefing on Capitol Hill sponsored by U.S. Senator Jeanne Shaheen ...

  14. FLINT HILLS RESOURES, LP- FE DKT NO. 15-169-LNG

    Energy.gov [DOE]

    The Office of Fossil Energy gives notice of receipt of an application filed on November 5, 2015, by Flint Hills Resources,  LP requesting  long-term, multi-contract authorization to export up to 12...

  15. Geothermal exploration program, Hill Air Force Base, Davis and Weber Counties, Utah

    SciTech Connect

    Glenn, W.E.; Chapman, D.S.; Foley, D.; Capuano, R.M.; Cole, D.; Sibbett, B.; Ward, S.H.

    1980-03-01

    Results obtained from a program designed to locate a low- or moderate-temperature geothermal resource that might exist beneath Hill Air Force Base (AFB), Ogden, Utah are discussed. A phased exploration program was conducted at Hill AFB. Published geological, geochemical, and geophysical reports on the area were examined, regional exploration was conducted, and two thermal gradient holes were drilled. This program demonstrated that thermal waters are not present in the shallow subsurface at this site. (MHR)

  16. Peer Review of the Hot Dry Rock Project at Fenton Hill, New Mexico

    SciTech Connect

    1998-12-01

    This report briefly describes the history of the hot dry rock experiment project conducted by the U.S. Department of Energy and Los Alamos National Laboratory at Fenton Hill, New Mexico, from about 1971 through 1995. The authors identify the primary lessons learned and techniques developed during the course of the Fenton Hill project, and summarize the extent to which these technologies have been transferred to the U.S. geothermal industry.

  17. NNSA takes part in 'DHS Day on the Hill' | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration | (NNSA) takes part in 'DHS Day on the Hill' Wednesday, January 22, 2014 - 3:36pm NNSA's Office of Emergency Operations participated in "DHS Day on the Hill," sponsored by the House Committee on Homeland Security, last week at the Cannon House Office Building. The event was an opportunity for each respective Department of Homeland Security component to demonstrate and showcase to members of Congress their unique capabilities and projects which contribute to the broad

  18. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    SciTech Connect

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation of the

  19. Voluntary Protection Program Onsite Review, CH2M HILL Plateau Remediation Co., Inc., Hanford – Jan 2014

    Energy.gov [DOE]

    Evaluation to determine whether CH2M HILL Plateau Remediation Co., Inc., Hanford is performing at a level deserving DOE-VPP Star recognition.

  20. miR-92a family and their target genes in tumorigenesis and metastasis

    SciTech Connect

    Li, Molin; Guan, Xingfang; Sun, Yuqiang; Mi, Jun; Shu, Xiaohong; Liu, Fang; Li, Chuangang

    2014-04-15

    The miR-92a family, including miR-25, miR-92a-1, miR-92a-2 and miR-363, arises from three different paralog clusters miR-17-92, miR-106a-363, and miR-106b-25 that are highly conservative in the process of evolution, and it was thought as a group of microRNAs (miRNAs) correlated with endothelial cells. Aberrant expression of miR-92a family was detected in multiple cancers, and the disturbance of miR-92a family was related with tumorigenesis and tumor development. In this review, the progress on the relationship between miR-92a family and their target genes and malignant tumors will be summarized. - Highlights: • Aberrant expression of miR-92a, miR-25 and miR-363 can be observed in many kinds of malignant tumors. • The expression of miR-92a family is regulated by LOH, epigenetic alteration, transcriptional factors such as SP1, MYC, E2F, wild-type p53 etc. • Roles of miR-92a family in tumorigenesis and development: promoting cell proliferation, invasion and metastasis, inhibiting cell apoptosis.

  1. U.S. strategic petroleum reserve Big Hill 114 leak analysis 2012.

    SciTech Connect

    Lord, David L.; Roberts, Barry L.; Lord, Anna C. Snider; Sobolik, Steven Ronald; Park, Byoung Yoon; Rudeen, David Keith

    2013-06-01

    This report addresses recent well integrity issues related to cavern 114 at the Big Hill Strategic Petroleum Reserve site. DM Petroleum Operations, M&O contractor for the U.S. Strategic Petroleum Reserve, recognized an apparent leak in Big Hill cavern well 114A in late summer, 2012, and provided written notice to the State of Texas as required by law. DM has since isolated the leak in well A with a temporary plug, and is planning on remediating both 114 A- and B-wells with liners. In this report Sandia provides an analysis of the apparent leak that includes: (i) estimated leak volume, (ii) recommendation for operating pressure to maintain in the cavern between temporary and permanent fixes for the well integrity issues, and (iii) identification of other caverns or wells at Big Hill that should be monitored closely in light of the sequence of failures there in the last several years.

  2. Conversion of the Big Hill geological site characterization report to a three-dimensional model.

    SciTech Connect

    Stein, Joshua S.; Rautman, Christopher Arthur

    2003-02-01

    The Big Hill salt dome, located in southeastern Texas, is home to one of four underground oil-storage facilities managed by the U. S. Department of Energy Strategic Petroleum Reserve (SPR) Program. Sandia National Laboratories, as the geotechnical advisor to the SPR, conducts site-characterization investigations and other longer-term geotechnical and engineering studies in support of the program. This report describes the conversion of two-dimensional geologic interpretations of the Big Hill site into three-dimensional geologic models. The new models include the geometry of the salt dome, the surrounding sedimentary units, mapped faults, and the 14 oil storage caverns at the site. This work provides a realistic and internally consistent geologic model of the Big Hill site that can be used in support of future work.

  3. DOE Tour of Zero: Vision Hill Lot 1 by Mandalay Homes | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Vision Hill Lot 1 by Mandalay Homes DOE Tour of Zero: Vision Hill Lot 1 by Mandalay Homes 1 of 8 Mandalay Homes built this 3,181-square-foot home in Glendale, Arizona, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 8 This one-level high-efficiency home meets the criteria of the DOE ZERH, ENERGY STAR, Indoor airPLUS, EPA WaterSense, and LEED for Homes Silver programs. 3 of 8 Tiles combine with stone to form pervious surfaces

  4. Port Huron, MI Natural Gas Pipeline Imports From Canada (Million...

    Energy Information Administration (EIA) (indexed site)

    Million Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 262 - No Data Reported; ...

  5. miR-107 and miR-25 simultaneously target LATS2 and regulate proliferation and invasion of gastric adenocarcinoma (GAC) cells

    SciTech Connect

    Zhang, Mingjun; Wang, Xiaolei; Li, Wanhu; Cui, Yongchun

    2015-05-08

    Although a series of oncogenes and tumor suppressors were identified in the pathological development of gastric adenocarcinoma (GAC), the underlying molecule mechanism were still not fully understood. The current study explored the expression profile of miR-107 and miR-25 in GAC patients and their downstream regulative network. qRT-PCR analysis was performed to quantify the expression of these two miRNAs in serum samples from both patients and healthy controls. Dual luciferase assay was conducted to verify their putative bindings with LATS2. MTT assay, cell cycle assay and transwell assay were performed to explore how miR-107 and miR-25 regulate proliferation and invasion of gastric cancer cells. Findings of this study demonstrated that total miR-107 or miR-25 expression might be overexpressed in gastric cancer patients and they can simultaneously and synchronically regulate LATS2 expression, thereby affecting gastric cancer cell growth and invasion. Therefore, the miR-25/miR-107-LATS2 axis might play an important role in proliferation and invasion of the gastric cancer cells. - Highlights: • Total miR-107 and miR-25 expression is significantly increased in GAC patients. • Both miR-107 and miR-25 can promote proliferation and invasion of GAC cells. • Both miR-107 and miR-25 can target LATS2 and regulate its expression. • miR-107 and miR-25 regulate proliferation and invasion of GAC cells though LATS2.

  6. miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression

    SciTech Connect

    Gao, Yong; Luo, Ling-hui; Li, Shuai; Yang, Cao

    2014-02-07

    Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.

  7. Preliminary of Violation, Kaiser-Hill Company, LLC,- EA-2004-02

    Office of Energy Efficiency and Renewable Energy (EERE)

    Issued to Kaiser-Hill Company, LLC for violations of 10 C.F.R. Parts 830 and 835 associated with a March 2003 radioactive material uptake, a March 2003 building airflow reversal event, a May 2003 glovebox fire, and Basis for Interim Operation/Technical Safety Requirements issues at the Rocky Flats Environmental Technology Site.

  8. EA-1967: Hills Creek-Lookout Point Transmission Line Rebuild, Lane County, Oregon

    Energy.gov [DOE]

    Bonneville Power Administration is preparing an EA to assess potential environmental impacts of the proposed rebuild of its 26-mile 115 kilovolt (kV) wood-pole Hills Creek-Lookout Point transmission line, which is generally located between Lowell and Oakridge, in Lane County, Oregon.

  9. VBA-0033- In the Matter of Kaiser-Hill Company, L.L.C.

    Energy.gov [DOE]

    On August 26 and 27, 1999, Kaiser-Hill Company, L.L.C. (K-H) and EG&G Rocky Flats, Inc. (EG&G) respectively filed Notices of Appeal from an Initial Agency Decision by a Hearing Officer from...

  10. MiR-218 Mediates tumorigenesis and metastasis: Perspectives and implications

    SciTech Connect

    Lu, Ying-fei; Zhang, Li; Waye, Mary Miu Yee; Fu, Wei-ming; Zhang, Jin-fang

    2015-05-15

    MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. As a highly conserved miRNA across a variety of species, microRNA-218 (miR-218) was found to play pivotal roles in tumorigenesis and progression. A group of evidence has demonstrated that miR-218 acts as a tumor suppressor by targeting many oncogenes related to proliferation, apoptosis and invasion. In this review, we provide a complex overview of miR-218, including its regulatory mechanisms, known functions in cancer and future challenges as a potential therapeutic target in human cancers. - Highlights: • miR-218 is frequently down regulated in multiple cancers. • miR-218 plays pivotal roles in carcinogenesis. • miR-218 mediates proliferation, apoptosis, metastasis, invasion, etc. • miR-218 mediates tumorigenesis and metastasis via multiple pathways.

  11. NuMI Low Energy Flux Prediction Release

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    NuMI Low Energy Flux Prediction Release Neutrino Flux Predictions for the NuMI Beam hep-ex/1607.00704 Data Ancillary data files for this result are available on arXiv at http://arxiv.org/src/1607.00704/anc Among the available data files are: pdf file describing format of all the available files root file of all the available fluxes python code to read and process MINERvA's flux predictions Text Files of the flux, uncertainties, and covariance matrix, with units of neutrinos/m^2/POT, in 0.5 GeV

  12. miRNA-205 affects infiltration and metastasis of breast cancer

    SciTech Connect

    Wang, Zhouquan; Department of Tumor, SenGong Hospital of Shaanxi, Xian 710300 ; Liao, Hehe; Deng, Zhiping; Yang, Po; Du, Ning; Zhanng, Yunfeng; Ren, Hong

    2013-11-08

    Highlights: We detected expression of miR-205 in breast cancer cell lines and tissue samples. We suggest miR-205 is downregulated in human breast cancer tissues and MCF7 cells. We suggest the lower expression of miR-205 play a role in breast cancer onset. These data suggest that miR-205 directly targets HER3 in human breast cancer. -- Abstract: Background: An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy. Methods: The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. Results: miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3? untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression. Conclusion: miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.

  13. Ground Motion Studies at NuMI

    SciTech Connect

    Mayda M. Velasco; Michal Szleper

    2012-02-20

    Ground motion can cause significant deterioration in the luminosity of a linear collider. Vibration of numerous focusing magnets causes continuous misalignments, which makes the beam emittance grow. For this reason, understanding the seismic vibration of all potential LC sites is essential and related efforts in many sites are ongoing. In this document we summarize the results from the studies specific to Fermilab grounds as requested by the LC project leader at FNAL, Shekhar Mishra in FY04-FY06. The Northwestern group focused on how the ground motion effects vary with depth. Knowledge of depth dependence of the seismic activity is needed in order to decide how deep the LC tunnel should be at sites like Fermilab. The measurements were made in the NuMI tunnel, see Figure 1. We take advantage of the fact that from the beginning to the end of the tunnel there is a height difference of about 350 ft and that there are about five different types of dolomite layers. The support received allowed to pay for three months of salary of Michal Szleper. During this period he worked a 100% of his time in this project. That include one week of preparation: 2.5 months of data taking and data analysis during the full period of the project in order to guarantee that we were recording high quality data. We extended our previous work and made more systematic measurements, which included detailed studies on stability of the vibration amplitudes at different depths over long periods of time. As a consequence, a better control and more efficient averaging out of the daytime variation effects were possible, and a better study of other time dependences before the actual depth dependence was obtained. Those initial measurements were made at the surface and are summarized in Figure 2. All measurements are made with equipment that we already had (two broadband seismometers KS200 from GEOTECH and DL-24 portable data recorder). The offline data analysis took advantage of the full Fourier spectra

  14. Science on the Hill: Driving toward an algae-powered future

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science on the Hill: Driving toward an algae-powered future Driving toward an algae-powered future A new research project led by Los Alamos National Laboratory seeks to drive algal biofuels to marketability, decreasing our nation's dependence on fossil fuels and putting the brakes on global warming. December 24, 2015 LANL scientist Richard Sayre Los Alamos National Laboratory scientist David Fox holds a vial of blue-green algae that is part of the Laboratory's research into improving algae

  15. Science on the Hill: Fragile life underfoot has big impact on desert

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Fragile life underfoot has big impact on desert Fragile life underfoot has big impact on desert Anyone who spends time in the high-desert landscape of Northern New Mexico has come across biological soil crusts, or biocrusts. This fragile crust fills a pivotal ecosystem niche. However, its survival is being challenged by threats from climate change and man-made disturbance. June 13, 2016 Science on the Hill: Fragile life underfoot has big impact on desert For years, space scientists thought the

  16. Science on the Hill: Rapid diagnosis a new weapon against re-emerging TB

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Rapid diagnosis a new weapon against re-emerging TB Rapid diagnosis a new weapon against re-emerging TB Researchers at Los Alamos National Laboratory have developed an innovative tool set for the early and accurate diagnosis of tuberculosis. June 7, 2015 March 1, 2015 Los Alamos biomedical scientist Harshini Mukundan. Los Alamos biomedical scientist Harshini Mukundan Los Alamos biomedical scientist Harshini Mukundan Science on the Hill: Rapid diagnosis a new weapon against re-emerging TB Around

  17. The U.S. Department of Energy and contractor CH2M HILL Plateau Remediation

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    6 . The U.S. Department of Energy and contractor CH2M HILL Plateau Remediation Company manage the Waste Encapsulation and Storage Facility at the Hanford Site in southeast Washington state. Waste Encapsulation and Storage Facility Background The Waste Encapsulation and Storage Facility (WESF) provides safe and compliant underwater storage for 1,936 highly radioactive capsules containing the elements cesium and strontium. In the 1970s, radioactive isotopes of the chemical elements cesium and

  18. 3D Model of the McGinness Hills Geothermal Area

    DOE Data Explorer

    Faulds, James E.

    2013-12-31

    The McGinness Hills geothermal system lies in a ~8.5 km wide, north-northeast trending accommodation zone defined by east-dipping normal faults bounding the Toiyabe Range to the west and west-dipping normal faults bounding the Simpson Park Mountains to the east. Within this broad accommodation zone lies a fault step-over defined by north-northeast striking, west-dipping normal faults which step to the left at roughly the latitude of the McGinness Hills geothermal system. The McGinness Hills 3D model consists of 9 geologic units and 41 faults. The basal geologic units are metasediments of the Ordovician Valmy and Vininni Formations (undifferentiated in the model) which are intruded by Jurassic granitic rocks. Unconformably overlying is a ~100s m-thick section of Tertiary andesitic lava flows and four Oligocene-to-Miocene ash-flow tuffs: The Rattlesnake Canyon Tuff, tuff of Sutcliffe, the Cambell Creek Tuff and the Nine Hill tuff. Overlying are sequences of pre-to-syn-extensional Quaternary alluvium and post-extensional Quaternary alluvium. 10-15 eastward dip of the Tertiary stratigraphy is controlled by the predominant west-dipping fault set. Geothermal production comes from two west dipping normal faults in the northern limb of the step over. Injection is into west dipping faults in the southern limb of the step over. Production and injection sites are in hydrologic communication, but at a deep level, as the northwest striking fault that links the southern and northern limbs of the step-over has no permeability.

  19. 3D Model of the McGinness Hills Geothermal Area

    DOE Data Explorer

    Faulds, James E.

    2013-12-31

    The McGinness Hills geothermal system lies in a ~8.5 km wide, north-northeast trending accommodation zone defined by east-dipping normal faults bounding the Toiyabe Range to the west and west-dipping normal faults bounding the Simpson Park Mountains to the east. Within this broad accommodation zone lies a fault step-over defined by north-northeast striking, west-dipping normal faults which step to the left at roughly the latitude of the McGinness Hills geothermal system. The McGinness Hills 3D model consists of 9 geologic units and 41 faults. The basal geologic units are metasediments of the Ordovician Valmy and Vininni Formations (undifferentiated in the model) which are intruded by Jurassic granitic rocks. Unconformably overlying is a ~100s m-thick section of Tertiary andesitic lava flows and four Oligocene-to-Miocene ash-flow tuffs: The Rattlesnake Canyon Tuff, tuff of Sutcliffe, the Cambell Creek Tuff and the Nine Hill tuff. Overlying are sequences of pre-to-syn-extensional Quaternary alluvium and post-extensional Quaternary alluvium. 10-15º eastward dip of the Tertiary stratigraphy is controlled by the predominant west-dipping fault set. Geothermal production comes from two west dipping normal faults in the northern limb of the step over. Injection is into west dipping faults in the southern limb of the step over. Production and injection sites are in hydrologic communication, but at a deep level, as the northwest striking fault that links the southern and northern limbs of the step-over has no permeability.

  20. DOE - Office of Legacy Management -- Mitts-Merrill Co - MI 14

    Office of Legacy Management (LM)

    1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces ... Primary Radioactive Materials Handled: Thorium MI.14-1 Radiological Survey(s): Yes - ...

  1. DOE - Office of Legacy Management -- Mitts-Merrel Co - MI 14

    Office of Legacy Management (LM)

    1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces ... Primary Radioactive Materials Handled: Thorium MI.14-1 Radiological Survey(s): Yes - ...

  2. Constraint satisfaction using a hybrid evolutionary hill-climbing algorithm that performs opportunistic arc and path revision

    SciTech Connect

    Bowen, J.; Dozier, G.

    1996-12-31

    This paper introduces a hybrid evolutionary hill-climbing algorithm that quickly solves (Constraint Satisfaction Problems (CSPs)). This hybrid uses opportunistic arc and path revision in an interleaved fashion to reduce the size of the search space and to realize when to quit if a CSP is based on an inconsistent constraint network. This hybrid outperforms a well known hill-climbing algorithm, the Iterative Descent Method, on a test suite of 750 randomly generated CSPs.

  3. Surface Radiation Survey at the Shepley’s Hill Remediation Site, Devens, Massachusettes

    SciTech Connect

    J. R. Giles; C. P. Oertel; L. G. Roybal

    2009-09-01

    The Idaho National Laboratory (INL) provided technical support for ongoing environmental remediation activities at the Shepley’s Hill remediation site, near Devens, MA. The technical support included the completion of a radiation survey of naturally occurring radioactive materials (NORM) at Shepley’s Hill, Shepley’s Hill landfill cover, and Red Cove areas. The objective of the radiation survey was to assess the ability of the INL backpack sodium iodide spectroscopy (BaSIS) system to detect elevated levels of NORM that may be associated with radon-222 emanation from near surface and subsurface fractures in the area. It is postulated that these fracture zones provide subsurface conduits for the transport of environmental contaminants. As such, location of these fracture sets will proved EPA Region 1 with the means for completing the development of an accurate site conceptual model. The results of the radiological survey show that some of the radiological anomalies correlate with currently mapped rock outcrops; however, not all of the rock outcrops in the surveyed area have been mapped. As such, it is not conclusive that all of the radiological anomalies correspond with surface rock outcrops. EPA Region 1 intends to perform a more comprehensive correlation of the radiation data collected with the BaSIS system with additional data sets such as detailed bedrock structural mapping, 2-dimensional resistivity profiling, and high-resolution topographic mapping. The results of this effort will be used in consideration of designing a potential follow-on effort for mapping of radon.

  4. Subsurface water flow simulated for hill slopes with spatially dependent soil hydraulic characteristics

    SciTech Connect

    Sharma, M.L.; Luxmoore, R.J.; DeAngelis, R.; Ward, R.C.; Yeh, G.T.

    1987-08-01

    Water flow through hill slopes consisting of five soil layers, with varying spatial dependence in hydraulic characteristics in the lateral plane was simulated by solving Richards' equation in three dimensions under varying rainfall intensities and for two complexities of terrain. By concepts of similar media the variability in soil hydraulic characteristics was expressed by a single dimensionless parameter, the scaling factor ..cap alpha... The moments of log normally distributed ..cap alpha.. were set as: Mean = 1.0 and standard deviation = 1.0. Four cases of spatial dependence of ..cap alpha.. in the lateral plane were selected for simulation, using exponential variogram functions ranging in spatial structure from random (no spatial dependence) to large dependence (large correlation lengths). The simulations showed that the rates of subsurface flow from the 30/sup 0/ hillslope, during and following rainfall, were significantly enhanced with an increase in spatial dependence. Subsurface drainage was also increased with increases in rainfall intensity and slop complexity. For hill slopes the relative effects of spatial dependence in soil hydraulic characteristics was smaller with 30/sup 0/ horizontal pitching than without pitching. Hill slopes with a random distribution of hydraulic characteristics provided greater opportunity for soil units with differing water capacities to interact than in cases with spatially correlated distributions. This greater interaction is associated with a greater lag in subsurface flow generation. These studies illustrate some of the expected effects of spatial dependence of soil hydraulic characteristics of the integrated hydrologic response of land areas.

  5. MiR-125a TNF receptor-associated factor 6 to inhibit osteoclastogenesis

    SciTech Connect

    Guo, Li-Juan; Liao, Lan; Yang, Li; Li, Yu; Jiang, Tie-Jian

    2014-02-15

    MicroRNAs (miRNAs) play important roles in osteoclastogenesis and bone resorption. In the present study, we found that miR-125a was dramatically down-regulated during macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) induced osteoclastogenesis of circulating CD14+ peripheral blood mononuclear cells (PBMCs). Overexpression of miR-125a in CD14+ PBMCs inhibited osteoclastogenesis, while inhibition of miR-125a promoted osteoclastogenesis. TNF receptor-associated factor 6 (TRAF6), a transduction factor for RANKL/RANK/NFATc1 signal, was confirmed to be a target of miR-125a. EMSA and ChIP assays confirmed that NFATc1 bound to the promoter of the miR-125a. Overexpression of NFATc1 inhibited miR-125a transcription, and block of NFATc1 expression attenuated RANKL-regulated miR-125a transcription. Here, we reported that miR-125a played a biological function in osteoclastogenesis through a novel TRAF6/ NFATc1/miR-125a regulatory feedback loop. It suggests that regulation of miR-125a expression may be a potential strategy for ameliorating metabolic disease. - Highlights: • MiR-125a was significantly down-regulated in osteoclastogenesis of CD14+ PBMCs. • MiR-125a inhibited osteoclast differentiation by targeting TRAF6. • NFATc1 inhibited miR-125a transciption by binding to the promoter of miR-125a. • TRAF6/NFATc1 and miR-125a form a regulatory feedback loop in osteoclastogenesis.

  6. Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet...

    Energy Information Administration (EIA) (indexed site)

    (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 1 2014 1 1 1 1 2 1 1 1 1 1 2015 1 1...

  7. Neutron scattering study of spin ordering and stripe pinning in superconducting <mi>La>1.93<mi>Sr>0.07<mi>CuO>4

    SciTech Connect

    Jacobsen, H.; Zaliznyak, I. A.; Savici, A. T.; Winn, B. L.; Chang, S.; Hücker, M.; Gu, G. D.; Tranquada, J. M.

    2015-11-20

    The relationships among charge order, spin fluctuations, and superconductivity in underdoped cuprates remain controversial. We use neutron scattering techniques to study these phenomena in <mi>La>1.93<mi>Sr>0.07<mi>CuO>4 a superconductor with a transition temperature of Tc = 20 K. At T<< Tc, we find incommensurate spin fluctuations with a quasielastic energy spectrum and no sign of a gap within the energy range from 0.2 to 15 meV. A weak elastic magnetic component grows below ~ 10 K, consistent with results from local probes. Regarding the atomic lattice, we have discovered unexpectedly strong fluctuations of the CuO6 octahedra about Cu-O bonds, which are associated with inequivalent O sites within the CuO2 planes. Moreover, we observed a weak elastic (3 30) superlattice peak that implies a reduced lattice symmetry. The presence of inequivalent O sites rationalizes various pieces of evidence for charge stripe order in underdoped La2-xSrxCuO4. The coexistence of superconductivity with quasi-static spin-stripe order suggests the presence of intertwined orders; however, the rotation of the stripe orientation away from the Cu-O bonds might be connected with evidence for a finite gap at the nodal points of the superconducting gap function.

  8. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma

    SciTech Connect

    Xiong, Xinkui; Sun, Daoyi; Chai, Hao; Shan, Wengang; Yu, Yue; Pu, Liyong; Cheng, Feng

    2015-09-18

    The dysregulation of micro (mi)RNAs is associated with cancer development. The miRNA miR-145 is downregulated in intrahepatic cholangiocarcinoma (ICC); however, its precise role in tumor progression has not yet been elucidated. Novel (nua) kinase family (NUAK)1 functions as an oncogene in various cancers and is a putative target of miR-145 regulation. In this study, we investigated the regulation of NUAK1 by miR-145 in ICC. We found that miR-145 level was significantly decreased in ICC tissue and cell lines, which corresponded with an increase in NUAK1 expression. NUAK1 was found to be a direct target of miR-145 regulation. The overexpression of miR-145 in ICC cell lines inhibited proliferation, growth, and invasion by suppressing NUAK1 expression, which was associated with a decrease in Akt signaling and matrix metalloproteinase protein expression. Similar results were observed by inhibiting NUAK1 expression. These results demonstrate that miR-145 can prevent ICC progression by targeting NUAK1 and its downstream effectors, and can therefore be useful for clinical diagnosis and targeted therapy of ICC. - Highlights: • MiR-145 suppresses ICC proliferation and invasion abilities. • We demonstrated that miR-145 directly targets NUAK1 in ICC. • MiR-145 expression in ICC was associated with Akt signaling and MMPs expression.

  9. Radiosensitizing Effects of Ectopic miR-101 on Non-Small-Cell Lung Cancer Cells Depend on the Endogenous miR-101 Level

    SciTech Connect

    Chen, Susie; Wang Hongyan; Ng, Wooi Loon; Curran, Walter J.; Wang Ya

    2011-12-01

    Purpose: Previously, we showed that ectopic miR-101 could sensitize human tumor cells to radiation by targeting ATM and DNA-PK catalytic subunit (DNA-PKcs) to inhibit DNA repair, as the endogenous miR-101 levels are low in tumors in general. However, the heterogeneity of human cancers may result in an exception. The purpose of this study was to test the hypothesis that a few tumor cell lines with a high level of endogenous miR-101 would prove less response to ectopic miR-101. Methods and Materials: Fourteeen non-small-cell lung cancer (NSCLC) cell lines and one immortalized non-malignant lung epithelial cell line (NL20) were used for comparing endogenous miR-101 levels by real-time reverse transcription-polymerase chain reaction. Based on the different miR-101 levels, four cell lines with different miR-101 levels were chosen for transfection with a green fluorescent protein-lentiviral plasmid encoding miR-101. The target protein levels were measured by using Western blotting. The radiosensitizing effects of ectopic miR-101 on these NSCLC cell lines were determined by a clonogenic assay and xenograft mouse model. Results: The endogenous miR-101 level was similar or lower in 13 NSCLC cell lines but was 11-fold higher in one cell line (H157) than in NL20 cells. Although ectopic miR-101 efficiently decreased the ATM and DNA-PKcs levels and increased the radiosensitization level in H1299, H1975, and A549 cells, it did not change the levels of the miR-101 targets or radiosensitivity in H157 cells. Similar results were observed in xenograft mice. Conclusions: A small number of NSCLC cell lines could have a high level of endogenous miR-101. The ectopic miR-101 was able to radiosensitize most NSCLC cells, except for the NSCLC cell lines that had a much higher endogenous miR-101 level. These results suggest that when we choose one miRNA as a therapeutic tool, the endogenous level of the miRNA in each tumor should be considered.

  10. City of Auburn, IN Smart Grid Project | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    system, including installing a smart meter network, enhancing reliable and fast communication capabilities, upgrading cyber security technologies, expanding grid monitoring and...

  11. PPARγ inhibits ovarian cancer cells proliferation through upregulation of miR-125b

    SciTech Connect

    Luo, Shuang; Wang, Jidong; Ma, Ying; Yao, Zhenwei; Pan, Hongjuan

    2015-06-26

    miR-125b has essential roles in coordinating tumor proliferation, angiogenesis, invasiveness, metastasis and chemotherapy recurrence. In ovarian cancer miR-125b has been shown to be downregulated and acts as a tumor suppressor by targeting proto-oncogene BCL3. PPARγ, a multiple functional transcription factor, has been reported to have anti-tumor effects through inhibition of proliferation and induction of differentiation and apoptosis by targeting the tumor related genes. However, it is unclear whether miR-125b is regulated by PPARγ in ovarian cancer. In this study, we demonstrated that the miR-125b downregulated in ovarian cancer tissues and cell lines. Ligands-activated PPARγ suppressed proliferation of ovarian cancer cells and this PPARγ-induced growth inhibition is mediated by the upregulation of miR-125b. PPARγ promoted the expression of miR-125b by directly binding to the responsive element in miR-125b gene promoter region. Thus, our results suggest that PPARγ can induce growth suppression of ovarian cancer by upregulating miR-125b which inhibition of proto-oncogene BCL3. These findings will extend our understanding of the function of PPARγ in tumorigenesis and miR-125b may be a therapeutic intervention of ovarian cancer. - Highlights: • miR-125b is down-regulated in ovarian cancer tissues and cells. • PPARγ upregulates miR-125b and downregulates its target gene BCL3 expression. • Silence of miR-125b attenuates PPARγ-mediated growth suppression of ovarian cancer cells. • PPARγ promotes the transcription of miR-125b via binding to PPARE in miR-125b gene promoter region.

  12. Pumped Storage Hydropower (Project Development Support)—Geotechnical Investigation and Value Stream Analysis for the Iowa Hill Pumped-Storage Development

    Office of Energy Efficiency and Renewable Energy (EERE)

    Pumped Storage Hydropower (Project Development Support)—Geotechnical Investigation and Value Stream Analysis for the Iowa Hill Pumped-Storage Development

  13. Application of turbidite facies of the Stevens Oil Zone for reservoir management, Elk Hills Field, California

    SciTech Connect

    Reid, S.A.; Thompson, T.W.; McJannet, G.S.

    1996-12-31

    A detailed depositional model for the uppermost sand reservoirs of the Stevens Oil Zone, Elk Hills Field, California, contains three facies: turbidite channel-fill sand bodies, overbank Sandstone and mudstone, and pelagic and hemipelagic siliceous shale. Sand bodies are the primary producing facies and consist of layered, graded sandstone with good permeability. The presence of incipient anticlines with subsea relief in the late Miocene resulted in deposition of lenticular and sinuous sand Was within structurally created channels. Relief of these structural channels was low when the earliest sand bodies were deposited, leading to a wide channel complex bounded by broad overbank deposits of moderate to low permeability. As deposition proceeded, increased structural relief constrained the channels, resulting in narrower sand body width and relatively abrupt channel terminations against very low permeability siliceous shale. With post-Miocene uplift and differential compaction, stratigraphic mounding of sand bodies helped create structural domes such as the 24Z reservoir. Stratigraphic traps including the 26R reservoir were also created. Such traps vary in seal quality from very effective to leaky, depending on the lateral transition from sand bodies to siliceous shale. Application of the Elk Hills turbidity model (1) provides a framework for monitoring production performance in the 24Z and Northwest Stevens waterflood projects; and for tracking gas migration into and out of the 26R reservoir, (2) helps b identify undeveloped locations in the 26R reservoir ideally suited for horizontal wells, (3) has led to the identification of two new production trends in the 29R area, and (4) makes possible the development of exploration plays in western Elk Hills.

  14. Application of turbidite facies of the Stevens Oil Zone for reservoir management, Elk Hills Field, California

    SciTech Connect

    Reid, S.A.; Thompson, T.W. ); McJannet, G.S. )

    1996-01-01

    A detailed depositional model for the uppermost sand reservoirs of the Stevens Oil Zone, Elk Hills Field, California, contains three facies: turbidite channel-fill sand bodies, overbank Sandstone and mudstone, and pelagic and hemipelagic siliceous shale. Sand bodies are the primary producing facies and consist of layered, graded sandstone with good permeability. The presence of incipient anticlines with subsea relief in the late Miocene resulted in deposition of lenticular and sinuous sand Was within structurally created channels. Relief of these structural channels was low when the earliest sand bodies were deposited, leading to a wide channel complex bounded by broad overbank deposits of moderate to low permeability. As deposition proceeded, increased structural relief constrained the channels, resulting in narrower sand body width and relatively abrupt channel terminations against very low permeability siliceous shale. With post-Miocene uplift and differential compaction, stratigraphic mounding of sand bodies helped create structural domes such as the 24Z reservoir. Stratigraphic traps including the 26R reservoir were also created. Such traps vary in seal quality from very effective to leaky, depending on the lateral transition from sand bodies to siliceous shale. Application of the Elk Hills turbidity model (1) provides a framework for monitoring production performance in the 24Z and Northwest Stevens waterflood projects; and for tracking gas migration into and out of the 26R reservoir, (2) helps b identify undeveloped locations in the 26R reservoir ideally suited for horizontal wells, (3) has led to the identification of two new production trends in the 29R area, and (4) makes possible the development of exploration plays in western Elk Hills.

  15. Study of Reservoir Heterogencities and Structural Features Affecting Production in the Shallow Oil Zone, Eastern Elk Hills Area, California

    SciTech Connect

    Janice Gillespie

    2004-11-01

    Late Neogene (Plio-Pleistocene) shallow marine strata of the western Bakersfield Arch and Elk Hills produce hydrocarbons from several different reservoirs. This project focuses on the shallow marine deposits of the Gusher and Calitroleum reservoirs in the Lower Shallow Oil Zone (LSOZ). In the eastern part of the study area on the Bakersfield Arch at North and South Coles Levee field and in two wells in easternmost Elk Hills, the LSOZ reservoirs produce dry (predominantly methane) gas. In structurally higher locations in western Elk Hills, the LSOZ produces oil and associated gas. Gas analyses show that gas from the eastern LSOZ is bacterial and formed in place in the reservoirs, whereas gas associated with oil in the western part of the study area is thermogenic and migrated into the sands from deeper in the basin. Regional mapping shows that the gas-bearing LSOZ sands in the Coles Levee and easternmost Elk Hills area are sourced from the Sierra Nevada to the east whereas the oil-bearing sands in western Elk Hills appear to be sourced from the west. The eastern Elk Hills area occupied the basin depocenter, farthest from either source area. As a result, it collected mainly low-permeability offshore shale deposits. This sand-poor depocenter provides an effective barrier to the updip migration of gases from east to west. The role of small, listric normal faults as migration barriers is more ambiguous. Because our gas analyses show that the gas in the eastern LSOZ reservoirs is bacterial, it likely formed in-place near the reservoirs and did not have to migrate far. Therefore, the gas could have been generated after faulting and accumulated within the fault blocks as localized pools. However, bacterial gas is present in both the eastern AND western parts of Elk Hills in the Dry Gas Zone (DGZ) near the top of the stratigraphic section even though the measured fault displacement is greatest in this zone. Bacterial gas is not present in the west in the deeper LSOZ which

  16. Slip and Dilation Tendency Anlysis of McGinness Hills Geothermal Area

    DOE Data Explorer

    Faulds, James E.

    2013-12-31

    Stress field variation within each focus area was approximated based on regional published data and the world stress database (Hickman et al., 2000; Hickman et al., 1998 Robertson-Tait et al., 2004; Hickman and Davatzes, 2010; Davatzes and Hickman, 2006; Blake and Davatzes 2011; Blake and Davatzes, 2012; Moeck et al., 2010; Moos and Ronne, 2010 and Reinecker et al., 2005) as well as local stress information if applicable. For faults within these focus systems we applied either a normal faulting stress regime where the vertical stress (sv) is larger than the maximum horizontal stress (shmax) which is larger than the minimum horizontal stress (sv>shmax>shmin) or strike-slip faulting stress regime where the maximum horizontal stress (shmax) is larger than the vertical stress (sv) which is larger than the minimum horizontal stress (shmax >sv>shmin) depending on the general tectonic province of the system. Based on visual inspection of the limited stress magnitude data in the Great Basin we used magnitudes such that shmin/shmax = .527 and shmin/sv= .46, which are consistent with complete and partial stress field determinations from Desert Peak, Coso, the Fallon area and Dixie valley (Hickman et al., 2000; Hickman et al., 1998 Robertson-Tait et al., 2004; Hickman and Davatzes, 2011; Davatzes and Hickman, 2006; Blake and Davatzes 2011; Blake and Davatzes, 2012). Slip and dilation tendency for the McGinness Hills geothermal field was calculated based on the faults mapped McGinness Hills area (Siler 2012, unpublished). The McGinness Hills area lies in the Basin and Range Province, as such we applied a normal faulting stress regime to the McGinness area faults, with a minimum horizontal stress direction oriented 115, based on inspection of local and regional stress determinations, as explained above. Under these stress conditions north-northeast striking, steeply dipping fault segments have the highest dilation tendency, while north-northeast striking 60 dipping fault

  17. An implementation of Hill's theory of normal anisotropic plasticity for explicit shell analysis

    SciTech Connect

    Whirley, R.G.; Engelmann, B.E.

    1991-08-20

    This paper summarizes the formulation and numerical implementation of a general anisotropic elastic-plastic material model for shell analysis. The 1948 Hill yield function is presented and specialized to conditions of plane stress. Next, an unconditionally stable and fully vectorized numerical algorithm for this constitutive model is presented. Finally, the model is specialized to conditions of normal anisotropy, and the implementation in DYNA3D is discussed. This development in material modeling should substantially extend the applicability of DYNA3D for many sheet metal forming applications. Several large-scale sheet metal forming examples are presented to illustrate these new analysis capabilities. 9 refs.

  18. Science on the Hill: Quenching New Mexico's thirst with brackish water

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Quenching New Mexico's thirst with brackish water Quenching New Mexico's thirst with brackish water Whether today turns out damp or dry, drought is a fact of life in New Mexico. So where can we get more water? November 15, 2015 water Brackish, or salty, water may prove an invaluable liquid resource for New Mexico. Science on the Hill: Quenching New Mexico's thirst with brackish water Whether today turns out damp or dry, drought is a fact of life in New Mexico. With our rivers and aquifers

  19. HILL: The High-Intensity Laser Laboratory Core Team's Reply to Questions from the NNSA Experimental Facilities Panel

    SciTech Connect

    Albright, B J

    2012-08-02

    Question 1 - The type of physics regimes that HILL can access for weapons studies is quite interesting. The question that arises for the proposal team is what priority does this type of experimental data have versus data that can be obtained with NIF, and Z. How does HILL rank in priority compared to MARIE 1.0 in terms of the experimental data it will provide? We reiterate that isochoric heating experiments to be conducted with HILL are complementary to the high energy density physics experiments at NIF and Z and uniquely access states of matter that neither other facility can access. It is our belief that HILL will enable several important questions, e.g., as related to mix morphology, radiation transfer from corrugated surfaces, and equations of state, to be run to ground through carefully diagnosed, 'unit-physics' experiments. Such experiments will substantially improve confidence in our computer models and provide a rigorous science basis for certification. Question 2 - A secondary question relates to the interests of LLNL and SNL in the physics that HILL can address. This should be spelled out clearly. I would like to see the other labs be part of the discussion regarding how important this capability would be if built. Both sister Labs have a keen interest in the physics enabled by high-intensity, high-energy lasers, as evinced by the Z Petawatt and NIF ARC upgrades to their signature facilities. LANL scientists have teamed with scientists from both Laboratories in high-intensity laser 'first experiments' envisioned for HILL and we fully intend to continue these profitable discussions going forward. In the preparation of the HILL proposal, feedback was solicited from the broader HEDP and weapons science communities. The consensus view was that HILL filled a critical gap and that there was a need for a facility like HILL to address outstanding questions in weapons science. It was recognized that co-location of HILL with a facility such as MaRIE 1.0, Z, NIF, or

  20. Environmental assessment for the Strategic Petroleum Reserve Big Hill facility storage of commercial crude oil project, Jefferson County, Texas

    SciTech Connect

    1999-03-01

    The Big Hill SPR facility located in Jefferson County, Texas has been a permitted operating crude oil storage site since 1986 with benign environmental impacts. However, Congress has not authorized crude oil purchases for the SPR since 1990, and six storage caverns at Big Hill are underutilized with 70 million barrels of available storage capacity. On February 17, 1999, the Secretary of Energy offered the 70 million barrels of available storage at Big Hill for commercial use. Interested commercial users would enter into storage contracts with DOE, and DOE would receive crude oil in lieu of dollars as rental fees. The site could potentially began to receive commercial oil in May 1999. This Environmental Assessment identified environmental changes that potentially would affect water usage, power usage, and air emissions. However, as the assessment indicates, changes would not occur to a major degree affecting the environment and no long-term short-term, cumulative or irreversible impacts have been identified.

  1. Heat extracted from the long term flow test in the Fenton Hill HDR reservoir

    SciTech Connect

    Kruger, Paul; Robinson, Bruce

    1994-01-20

    A long-term flow test was carried out in the Fenton Hill HDR Phase-2 reservoir for 14 months during 1992-1993 to examine the potential for supplying thermal energy at a sustained rate as a commercial demonstration of HDR technology. The test was accomplished in several segments with changes in mean flowrate due to pumping conditions. Re-test estimates of the extractable heat content above a minimum useful temperature were based on physical evidence of the size of the Fenton Hill reservoir. A numerical model was used to estimate the extent of heat extracted during the individual flow segments from the database of measured production data during the test. For a reservoir volume of 6.5x10{sup 6}m{sup 3}, the total heat content above a minimum temperature of 150{degree} C was 1.5x10{sup 15}J. For the total test period at the three sustained mean flowrates, the integrated heat extracted was 0.088x10{sup 15}J, with no discernable temperature decline of the produced fluid. The fraction of energy extracted above the abandonment temperature was 5.9%. On the basis of a constant thermal energy extraction rate, the lifetime of the reservoir (without reservoir growth) to the abandonment temperature would be 13.3 years, in good agreement with the pre-test estimate of 15.0 years for the given reservoir volume.

  2. Seismicity Characterization and Monitoring at WESTCARB's Proposed Montezuma Hills Geologic Sequestration Site

    SciTech Connect

    Daley, T.M.; Haught, R.; Peterson, J.E.; Boyle, K.; Beyer, J.H.; Hutchings, L.R.

    2010-09-15

    The West Coast Regional Carbon Sequestration Partnership (WESTCARB), in collaboration with Shell Oil Co. performed site characterization for a potential small-scale pilot test of geologic sequestration of carbon dioxide (CO2). The site area, know as Montezuma Hills, is near the town of Rio Vista in northern California. During the process of injection at a CO2 storage site, there is a potential for seismic events due to slippage upon pre-existing discontinuities or due to creation of new fractures. Observations from many injection projects have shown that the energy from these events can be used for monitoring of processes in the reservoir. Typically, the events are of relatively high frequency and very low amplitude. However, there are also well documented (non-CO2-related) cases in which subsurface injection operations have resulted in ground motion felt by near-by communities. Because of the active tectonics in California (in particular the San Andreas Fault system), and the potential for public concern, WESTCARB developed and followed an induced seismicity protocol (Myer and Daley, 2010). This protocol called for assessing the natural seismicity in the area and deploying a monitoring array if necessary. In this report, we present the results of the natural seismicity assessment and the results of an initial temporary deployment of two seismometers at the Montezuma Hills site. Following the temporary array deployment, the project was suspended and the array removed in August of 2010.

  3. Genome-Wide Analysis of miRNA targets in Brachypodium and Biomass Energy Crops

    SciTech Connect

    Green, Pamela J.

    2015-08-11

    MicroRNAs (miRNAs) contribute to the control of numerous biological processes through the regulation of specific target mRNAs. Although the identities of these targets are essential to elucidate miRNA function, the targets are much more difficult to identify than the small RNAs themselves. Before this work, we pioneered the genome-wide identification of the targets of Arabidopsis miRNAs using an approach called PARE (German et al., Nature Biotech. 2008; Nature Protocols, 2009). Under this project, we applied PARE to Brachypodium distachyon (Brachypodium), a model plant in the Poaceae family, which includes the major food grain and bioenergy crops. Through in-depth global analysis and examination of specific examples, this research greatly expanded our knowledge of miRNAs and target RNAs of Brachypodium. New regulation in response to environmental stress or tissue type was found, and many new miRNAs were discovered. More than 260 targets of new and known miRNAs with PARE sequences at the precise sites of miRNA-guided cleavage were identified and characterized. Combining PARE data with the small RNA data also identified the miRNAs responsible for initiating approximately 500 phased loci, including one of the novel miRNAs. PARE analysis also revealed that differentially expressed miRNAs in the same family guide specific target RNA cleavage in a correspondingly tissue-preferential manner. The project included generation of small RNA and PARE resources for bioenergy crops, to facilitate ongoing discovery of conserved miRNA-target RNA regulation. By associating specific miRNA-target RNA pairs with known physiological functions, the research provides insights about gene regulation in different tissues and in response to environmental stress. This, and release of new PARE and small RNA data sets should contribute basic knowledge to enhance breeding and may suggest new strategies for improvement of biomass energy crops.

  4. DLEU2, frequently deleted in malignancy, functions as a critical host gene of the cell cycle inhibitory microRNAs miR-15a and miR-16-1

    SciTech Connect

    Lerner, Mikael; Harada, Masako; Loven, Jakob; Castro, Juan; Davis, Zadie; Oscier, David; Henriksson, Marie; Sangfelt, Olle; Grander, Dan; Corcoran, Martin M.

    2009-10-15

    The microRNAs miR-15a and miR-16-1 are downregulated in multiple tumor types and are frequently deleted in chronic lymphocytic leukemia (CLL), myeloma and mantle cell lymphoma. Despite their abundance in most cells the transcriptional regulation of miR-15a/16-1 remains unclear. Here we demonstrate that the putative tumor suppressor DLEU2 acts as a host gene of these microRNAs. Mature miR-15a/miR-16-1 are produced in a Drosha-dependent process from DLEU2 and binding of the Myc oncoprotein to two alterative DLEU2 promoters represses both the host gene transcript and levels of mature miR-15a/miR-16-1. In line with a functional role for DLEU2 in the expression of the microRNAs, the miR-15a/miR-16-1 locus is retained in four CLL cases that delete both promoters of this gene and expression analysis indicates that this leads to functional loss of mature miR-15a/16-1. We additionally show that DLEU2 negatively regulates the G1 Cyclins E1 and D1 through miR-15a/miR-16-1 and provide evidence that these oncoproteins are subject to miR-15a/miR-16-1-mediated repression under normal conditions. We also demonstrate that DLEU2 overexpression blocks cellular proliferation and inhibits the colony-forming ability of tumor cell lines in a miR-15a/miR-16-1-dependent way. Together the data illuminate how inactivation of DLEU2 promotes cell proliferation and tumor progression through functional loss of miR-15a/miR-16-1.

  5. Microfluidic Molecular Assay Platform for the Detection of miRNAs...

    Office of Scientific and Technical Information (OSTI)

    Article: Microfluidic Molecular Assay Platform for the Detection of miRNAs, mRNAs, Proteins, and Post-translational Modifications at Single-cell Resolution. Citation Details...

  6. DOE - Office of Legacy Management -- Baker-Perkins Co - MI 13

    Office of Legacy Management (LM)

    Site Disposition: Eliminated - Potential for contamination remote based on limited scope of activities at the site MI.13-3 Radioactive Materials Handled: Yes Primary ...

  7. miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A

    SciTech Connect

    Shao, Mingchen; Geng, Yiwei; Lu, Peng; Xi, Ying; Wei, Sidong; Wang, Liuxing; Fan, Qingxia; Ma, Wang

    2015-09-04

    MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention. - Highlights: • miR-4295 mimics promote the proliferation and invasion of ATC cells. • miR-4295 inhibitors inhibit the proliferation and invasion of ATC cells. • miR-4295 targets 3′UTR of CDKN1A in ATC cells. • miR-4295 negatively regulates CDKN1A in ATC cells.

  8. Mitigation action plan sale of Naval Petroleum Reserve No. 1 (Elk Hills) Kern County, California

    SciTech Connect

    1998-01-01

    Naval Petroleum Reserve No. 1 (NPR-1, also called {open_quotes}Elk Hills{close_quotes}), a Federally-owned oil and gas production field in Kern County, California, was created by an Executive Order issued by President Taft on September 2, 1912. He signed another Executive Order on December 13, 1912, to establish Naval Petroleum Reserve No. 2 (NPR-2), located immediately south of NPR-1 and containing portions of the town of Taft, California. NPR-1 was not developed until the 1973-74 oil embargo demonstrated the nation`s vulnerability to oil supply interruptions. Following the embargo, Congress passed the Naval Petroleum Reserves Production Act of 1976 which directed that the reserve be explored and developed to its fall economic potential at the {open_quotes}maximum efficient rate{close_quotes} (MER) of production. Since Elk Hills began full production in 1976, it has functioned as a commercial operation, with total revenues to the Federal government through FY 1996 of $16.4 billion, compared to total exploration, development and production costs of $3.1 billion. In February 1996, Title 34 of the National Defense Authorization Act for Fiscal Year 1996 (P.L. 104-106), referred to as the Elk Hills Sales Statute, directed the Secretary of Energy to sell NPR-1 by February 10, 1998.The Secretary was also directed to study options for enhancing the value of the other Naval Petroleum and Oil Shale Reserve properties such as NPR-2, located adjacent to NPR-1 in Kern County- Naval Petroleum Reserve No. 3 (NPR-3) located in Natrona County, Wyoming; Naval Oil Shale Reserves No. 1 and No. 3 (NOSR-1 and NOSR-3) located in Garfield County, Colorado; and Naval Oil Shale Reserve No. 2 (NOSR-2) located in Uintah and Carbon Counties, Utah. The purpose of these actions was to remove the Federal government from the inherently non-Federal function of operating commercial oil fields while making sure that the public would obtain the maximum value from the reserves.

  9. Groundwater protection for the NuMI project

    SciTech Connect

    Wehmann, A.; Smart, W.; Menary, S.; Hylen, J.; Childress, S.

    1997-10-01

    The physics requirements for the long base line neutrino oscillation experiment MINOS dictate that the NuMI beamline be located in the aquifer at Fermilab. A methodology is described for calculating the level of radioactivation of groundwater caused by operation of this beamline. A conceptual shielding design for the 750 meter long decay pipe is investigated which would reduce radioactivation of the groundwater to below government standards. More economical shielding designs to meet these requirements are being explored. Also, information on local geology, hydrogeology, government standards, and a glossary have been included.

  10. SREL Reprint #3293

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    3 Defining the reference condition for wadeable streams in the Sand Hills subdivision of the Southeastern Plains ecoregion, USA Ely Kosnicki1, Stephen A. Sefick1, Michael H. Paller2, Miller S. Jarrell1, Blair A. Prusha3, Sean C. Sterrett4, Tracey D. Tuberville5, and Jack W. Feminella1 1Department of Biological Sciences, 331 Funchess Hall, Auburn University, Auburn, AL 36849, USA 2Savannah River National Laboratory, Aiken, SC, USA 3Midwest Biodiversity Institute, 4673 Northwest Parkway,

  11. FEASIBILITY OF WIND TO SERVE UPPER SKAGIT'S BOW HILL TRIBAL LANDS AND FEASIBILITY UPDATE FOR RESIDENTIAL RENEWABLE ENERGY.

    SciTech Connect

    RICH, LAUREN

    2013-09-30

    A two year wind resource assessment was conducted to determine the feasibility of developing a community scale wind generation system for the Upper Skagit Indian Tribe's Bow Hill land base, and the project researched residential wind resource technologies to determine the feasibility of contributing renewable wind resource to the mix of energy options for our single and multi-family residential units.

  12. Start-up operations at the Fenton Hill HDR Pilot Plant

    SciTech Connect

    Ponden, R.F.

    1991-01-01

    With the completion of the surface test facilities at Fenton Hill, the Hot Dry Rock (HDR) Geothermal Energy Program at Los Alamos is moving steadily into the next stage of development. Start-up operations of the surface facilities have begun in preparation for testing the Phase II reservoir and the initial steady-state phase of operations. A test program has been developed that will entail a number of operational strategies to characterize the thermal performance of the reservoir. The surface facilities have been designed to assure high reliability while providing the flexibility and control to support the different operating modes. This paper presents a review of the system design and provides a discussion of the preliminary results of plant operations and equipment performance.

  13. Start-Up Operations at the Fenton Hill HDR Pilot Plant

    SciTech Connect

    Ponden, Raymond F.

    1992-03-24

    With the completion of the surface test facilities at Fenton Hill, the Hot Dry Rock (HDR) Geothermal Energy Program at Los Alamos is moving steadily into the next stage of development. Start-up operations of the surface facilities have begun in preparation for testing the Phase II reservoir and the initial steady-state phase of operations. A test program has been developed that will entail a number of operational strategies to characterize the thermal performance of the reservoir. The surface facilities have been designed to assure high reliability while providing the flexibility and control to support the different operating modes. This paper presents a review of the system design and provides a discussion of the preliminary results of plant operations and equipment performance.

  14. Evidence for a welded tuff in the Rhyolite of Calico Hills

    SciTech Connect

    Dickerson, R.P.; Hunter, W.C.

    1994-12-31

    A welded pyroclastic deposit has been identified in the Rhyolite of Calico Hills near Yucca Mountain, Nevada, where only lava flows and nonwelded pyroclastic deposits were previously described. Field data from Fortymile Wash show that nonwelded, bedded tuff grades upward into partially welded massive ruff, and thence into densely welded vitrophyre. Petrographic data show a progressive decrease in inter- and intragranular porosity and amount of vapor-phase minerals, with increasing welding. Pumice fragments are first deformed, then develop diffuse boundaries which become increasingly obscure with progressive welding. The most densely welded rock is a perlitic vitrophyre. The origin of this welded tuff is not clear, as it could represent an ignimbrite or a tuff fused beneath a thick lava flow.

  15. Naval Petroleum Reserve No. 1 (Elk Hills): Supplemental environmental impact statement. Record of decision

    SciTech Connect

    Not Available

    1994-02-01

    Pursuant to the Council on Environmental Quality regulations, which implement the procedural provisions of the National Environmental Policy Act, and the US Department of Energy National Environmental Policy Act regulations, the Department of Energy, Office of Fossil Energy, is issuing a Record of Decision on the continued operation of Naval Petroleum Reserve No. 1, Kern County, California. The Department of Energy has decided to continue current operations at Naval Petroleum Reserve No. 1 and implement additional well drilling, facility development projects and other activities necessary for continued production of Naval Petroleum Reserve No. 1 in accordance with the requirements of the Naval Petroleum Reserves Production Act of 1976. The final Supplemental Environmental Impact Statement, entitled ``Petroleum Production at Maximum Efficient Rate, Naval Petroleum Reserve No. 1 (Elk Hills), Kern County, California (DOE/SEIS-0158),`` was released on September 3, 1993.

  16. Crosshole EM for oil field characterization and EOR monitoring: Field examples from Lost Hills, California

    SciTech Connect

    Wilt, M.; Schenkel, C.; Wratcher, M.; Lambert, I.; Torres-Verdin, C.; Tseng H.W.

    1996-07-16

    A steamflood recently initiated by Mobil Development and Production U.S. at the Lost Hills No 3 oil field in California is notable for its shallow depth and the application of electromagnetic (EM) geophysical techniques to monitor the subsurface steam flow. Steam was injected into three stacked eastward-dipping unconsolidated oil sands at depths from 60 to 120 m; the plume is expected to develop as an ellipsoid aligned with the regional northwest-southeast strike. Because of the shallow depth of the sands and the high viscosity of the heavy oil, it is important to track the steam in the unconsolidated sediments for both economic and safety reasons. Crosshole and surface-to-borehole electromagnetic imaging were applied for reservoir characterization and steamflood monitoring. The crosshole EM data were collected to map the interwell distribution of the high-resistivity oil sands and to track the injected steam and hot water. Measurements were made in two fiberglass-cased observation wells straddling the steam injector on a northeast-southwest profile. Field data were collected before the steam drive, to map the distribution of the oil sands, and then 6 and 10 months after steam was injected, to monitor the expansion of the steam chest. Resistivity images derived from the collected data clearly delineated the distribution and dipping structure of the target oil sands. Difference images from data collected before and during steamflooding indicate that the steam chest has developed only in the middle and lower oil sands, and it has preferentially migrated westward in the middle oil sand and eastward in the deeper sand. Surface-to-borehole field data sets at Lost Hills were responsive to the large-scale subsurface structure but insufficiently sensitive to model steam chest development in the middle and lower oil sands. As the steam chest develops further, these data will be of more use for process monitoring.

  17. Dune-dammed lakes of the Nebraska Sand Hills: Geologic setting and paleoclimatic implications

    SciTech Connect

    Loope, D.B.; Swinehart, J.B. (Univ. of Nebraska, Lincoln, NE (United States))

    1992-01-01

    Within the western half of this grass-stabilized dunefield, about 1,000 interdune lakes are grouped into two clusters here named the Blue and Birdwood lake basins. In the lake basins, those parts of the valley not filled by dune sand are occupied by modern lakes and Holocene lake sediments. The Blue Creek dam is mounded transverse to flow; spill-over of the lake basin takes place over bedrock on the east side of the dam when lake level is 2 m higher than present. The permeability of dune sand prevents massive overflow, and thereby contributes to the integrity and longevity of the dam. Preserved lake sediments in the basin indicate that Blue Creek was obstructed prior to 13,000 yr BP, probably during glacial maximum (18,000 yr BP). Extensive peats dated at 1,500-1,000 yr BP lie directly on fluvial sand and gravel along the Calamus River, a stream that presently discharges a nearly constant 350 cfs. These sediments indicate blockage of streams also took place when linear dunes were active in the eastern Sand Hills in Late Holocene time. With the onset of an arid episode, dunes forming an interfluves curtail the severity of runoff events. As the regional water table drops, drainages go dry and dunes move uncontested into blocking positions. Although drainages of the eastern Sand Hills appear to have repeatedly broken through sand-blocked channels, the Blue and Birdwood lake basins are still blocked by Late Pleistocene dune dams. The repeated episodes of stream blockage and interbedded lake sediments and dune sands behind the extant dams record several strong fluctuations in Holocene climate. Recently proposed climatic models indicate that the northward flow of warm, moist air from the Gulf of Mexico is enhanced when the Gulf's surface temperature is low and the Bermuda high is intensified and in a western position. When the Bermuda high moves eastward, the core of the North American continent becomes desiccated.

  18. MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19

    SciTech Connect

    Shan, Nianxi; Shen, Liangfang; Wang, Jun; He, Dan; Duan, Chaojun

    2015-01-02

    Highlights: • Decreased miR-153 and up-regulated ADAM19 are correlated with NSCLC pathology. • MiR-153 inhibits the proliferation and migration and invasion of NSCLC cells in vitro. • ADAM19 is a direct target of miR-153. • ADAM19 is involved in miR-153-suppressed migration and invasion of NSCLC cells. - Abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3′UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which was similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.

  19. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma

    SciTech Connect

    Zhai, Jian; Qu, Shuping; Li, Xiaowei; Zhong, Jiaming; Chen, Xiaoxia; Qu, Zengqiang; Wu, Dong

    2015-08-14

    Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights: • Decreased of miR-129 is found in HCC and associated with advanced stage and metastasis. • miR-129 suppresses proliferation and invasion of HCC cells. • miR-129 directly targets the 3′ UTR of PAK5 and diminishes PAK5 expression. • PAK5 is involved in miR-129 mediated suppression functions.

  20. Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells

    SciTech Connect

    Li, Junxia; Shan, Fabo; Xiong, Gang; Wang, Ju-Ming; Wang, Wen-Lin; Xu, Xueqing; Bai, Yun

    2014-03-28

    Highlights: • MiR-146b promotes esophageal cancer cell proliferation. • MiR-146b inhibits esophageal cancer cell apoptosis. • C/EBPβ directly binds to miR-146b promoter conserved region. • MiR-146b is up-regulated by C/EBPβ LAP2 transcriptional activation. - Abstract: Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.

  1. miR-1297 mediates PTEN expression and contributes to cell progression in LSCC

    SciTech Connect

    Li, Xin; Wang, Hong-liang; Peng, Xin; Zhou, Hui-fang; Wang, Xin

    2012-10-19

    Highlights: Black-Right-Pointing-Pointer miR-1297 was found to be overexpressed in LSCC and contribute to the cell progression. Black-Right-Pointing-Pointer PTEN was confirmed to be a target gene of miR-1297. Black-Right-Pointing-Pointer Downregulation of PTEN can rescue the proliferation and invasion ability of miR-1297 downregulated Hep-2 cells. Black-Right-Pointing-Pointer Downregulation of miR-1297 inhibits tumor growth in vivo. -- Abstract: MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression after transcription, and are involved in cancer development. Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant neoplasms with increasing incidence in recent years. In this paper, we report the overexpression of miR-1297 in LSCC and Hep-2 cells. In addition, PTEN was identified to be directly regulated by miR-1297 through western blot and luciferase activity assay. Furthermore, downregulation of miR-1297 in Hep-2 cells was shown to inhibit cancer cell proliferation, migration, and tumor genesis. Our results document a new epigenetic mechanism for PTEN regulation in LSCC, which is crucial for the development of these tumors.

  2. miR-30a suppresses breast cancer cell proliferation and migration by targeting Eya2

    SciTech Connect

    Fu, Jing; Xu, Xiaojie; Kang, Lei; Zhou, Liying; Wang, Shibin; Lu, Juming; Cheng, Long; Fan, Zhongyi; Yuan, Bin; Tian, Peirong; Zheng, Xiaofei; Yu, Chengze; Ye, Qinong; Lv, Zhaohui

    2014-03-07

    Highlights: • miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. • The miR-30a/EYA2 axis regulates breast cancer cell proliferation and migration. • The miR-30a/EYA2 axis modulates G1/S cell cycle progression. • The miR-30a/EYA2 axis is dysregulated in breast cancer patients. - Abstract: Eye absent (Eya) proteins are involved in cell fate determination in a broad spectrum of cells and tissues. Aberrant expression of Eya2 has been documented in a variety of cancers and correlates with clinical outcome. However, whether microRNAs (miRNAs) can regulate Eya2 expression remains unknown. Here, we show that miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. Overexpression of Eya2 in miR-30a-transfected breast cancer cells effectively rescued the inhibition of cell proliferation and migration caused by miR-30a. Knockdown of Eya2 by small-interfering RNA (siRNA) in breast cancer cells mimicked the effect induced by miR-30a and abolished the ability of miR-30a to regulate breast cancer cell proliferation and migration. The miR-30a/Eya2 axis could regulate G1/S cell cycle progression, accompanied by the modulation of expression of cell cycle-related proteins, including cyclin A, cyclin D1, cyclin E, and c-Myc. Moreover, miR-30a expression was downregulated in breast cancer patients, and negatively correlated with Eya2, which was upregulated in breast cancer patients. These data suggest that the miR-30a/Eya2 axis may play an important role in breast cancer development and progression and that miR-30a activation or Eya2 inhibition may be a useful strategy for cancer treatment.

  3. Slip and Dilation Tendency Anlysis of McGinness Hills Geothermal Area

    DOE Data Explorer

    Faulds, James E.

    2013-12-31

    Slip and Dilation Tendency in focus areas Critically stressed fault segments have a relatively high likelihood of acting as fluid flow conduits (Sibson, 1994). As such, the tendency of a fault segment to slip (slip tendency; Ts; Morris et al., 1996) or to dilate (dilation tendency; Td; Ferrill et al., 1999) provides an indication of which faults or fault segments within a geothermal system are critically stressed and therefore likely to transmit geothermal fluids. The slip tendency of a surface is defined by the ratio of shear stress to normal stress on that surface: Ts = τ / σn (Morris et al., 1996). Dilation tendency is defined by the stress acting normal to a given surface: Td = (σ1-σn) / (σ1-σ3) (Ferrill et al., 1999). Slip and dilation were calculated using 3DStress (Southwest Research Institute). Slip and dilation tendency are both unitless ratios of the resolved stresses applied to the fault plane by ambient stress conditions. Values range from a maximum of 1, a fault plane ideally oriented to slip or dilate under ambient stress conditions to zero, a fault plane with no potential to slip or dilate. Slip and dilation tendency values were calculated for each fault in the focus study areas at, McGinness Hills, Neal Hot Springs, Patua, Salt Wells, San Emidio, and Tuscarora on fault traces. As dip is not well constrained or unknown for many faults mapped in within these we made these calculations using the dip for each fault that would yield the maximum slip tendency or dilation tendency. As such, these results should be viewed as maximum tendency of each fault to slip or dilate. The resulting along-fault and fault-to-fault variation in slip or dilation potential is a proxy for along fault and fault-to-fault variation in fluid flow conduit potential. Stress Magnitudes and directions Stress field variation within each focus area was approximated based on regional published data and the world stress database (Hickman et al., 2000; Hickman et al., 1998

  4. miR-21 modulates resistance of HR-HPV positive cervical cancer cells to radiation through targeting LATS1

    SciTech Connect

    Liu, Shikai; Song, Lili Zhang, Liang; Zeng, Saitian; Gao, Fangyuan

    2015-04-17

    Although multiple miRNAs are found involved in radioresistance development in HR-HPV positive (+) cervical cancer, only limited studies explored the regulative mechanism of the miRNAs. miR-21 is one of the miRNAs significantly upregulated in HR-HPV (+) cervical cancer is also significantly associated with radioresistance. However, the detailed regulative network of miR-21 in radioresistance is still not clear. In this study, we confirmed that miR-21 overexpression was associated with higher level of radioresistance in HR-HPV (+) cervical cancer patients and thus decided to further explore its role. Findings of this study found miR-21 can negatively affect radiosensitivity of HR-HPV (+) cervical cancer cells and decrease radiation induced G2/M block and increase S phase accumulation. By using dual luciferase assay, we verified a binding site between miR-21 and 3′-UTR of large tumor suppressor kinase 1 (LATS1). Through direct binding, miR-21 can regulate LATS1 expression in cervical cancer cells. LATS1 overexpression can reverse miR-21 induced higher colony formation rate and also reduced miR-21 induced S phase accumulation and G2/M phase block reduction under radiation treatment. These results suggested that miR-21-LATS1 axis plays an important role in regulating radiosensitivity. - Highlights: • miR-21 is highly expressed in HR-HPV (+) radioresistant cervical cancer patients. • miR-21 can negatively affect radiosensitivity of HR-HPV (+) cervical cancer cells. • miR-21 can decrease radiation induced G2/M block and increase S phase accumulation. • miR-21 modulates radiosensitivity cervical cancer cell by directly targeting LATS1.

  5. miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7

    SciTech Connect

    Zhou, Chuanyi; Shen, Liangfang; Mao, Lei; Wang, Bing; Li, Yang; Yu, Huizhi

    2015-02-27

    MicroRNAs (miRNAs) are involved in the cervical carcinogenesis and progression. In this study, we investigated the role of miR-92a in progression and invasion of cervical cancer. MiR-92a was significantly upregulated in cervical cancer tissues and cell lines. Overexpression of miR-92a led to remarkably enhanced proliferation by promoting cell cycle transition from G1 to S phase and significantly enhanced invasion of cervical cancer cells, while its knockdown significantly reversed these cellular events. Bioinformatics analysis suggested F-box and WD repeat domain-containing 7 (FBXW7) as a novel target of miR-92a, and miR-92a suppressed the expression level of FBXW7 mRNA by direct binding to its 3′-untranslated region (3′UTR). Expression of miR-92a was negatively correlated with FBXW7 in cervical cancer tissues. Furthermore, Silencing of FBXW7 counteracted the effects of miR-92a suppression, while its overexpression reversed oncogenic effects of miR-92a. Together, these findings indicate that miR-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, suggesting miR-92a as a potential novel diagnostic and therapeutic target of cervical cancer. - Highlights: • miR-92a is elevated in cervical cancer tissues and cell lines. • miR-92a promotes cervical cancer cell proliferation, cell cycle transition from G1 to S phase and invasion. • FBXW7 is a direct target of miR-92a. • FBXW7 counteracts the oncogenic effects of miR-92a on cervical cancer cells.

  6. Marysville, MI Natural Gas Pipeline Imports From Canada (Million Cubic

    Energy Information Administration (EIA) (indexed site)

    Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 10 1,827 135 2000's NA NA 74 0 303 0 24 876 2,252 5,651 2010's 5,694 9,946 8,099 2,337 4,650 1,961 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Natural Gas Pipeline Imports by Point of Entry Marysville, MI Natural Gas Imports by Pipeli

  7. Marysville, MI Natural Gas Pipeline Imports From Canada (Dollars per

    Energy Information Administration (EIA) (indexed site)

    Thousand Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 3.48 2.17 2.06 2000's NA NA 3.95 -- 7.80 -- 7.07 7.59 8.59 3.80 2010's 4.44 4.42 2.99 4.15 6.86 2.73 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Price of Natural Gas Pipeline Imports by Point of Entry Marysville, MI Natural Gas

  8. NuMI proton kicker extraction magnet termination resistor system

    SciTech Connect

    Reeves, S.R.; Jensen, C.C.; /Fermilab

    2005-05-01

    The temperature stability of the kicker magnet termination resistor assembly directly affects the field flatness and amplitude stability. Comprehensive thermal enhancements were made to the existing Main Injector resistor assembly design to satisfy NuMI performance specifications. Additionally, a fluid-processing system utilizing Fluorinert{reg_sign} FC-77 high-voltage dielectric was built to precisely control the setpoint temperature of the resistor assembly from 70 to 120F, required to maintain constant resistance during changing operational modes. The Fluorinert{reg_sign} must be continually processed to remove hazardous breakdown products caused by radiation exposure to prevent chemical attack of system components. Design details of the termination resistor assembly and Fluorinert{reg_sign} processing system are described. Early performance results will be presented.

  9. Non-canonical microRNAs miR-320 and miR-702 promote proliferation in Dgcr8-deficient embryonic stem cells

    SciTech Connect

    Kim, Byeong-Moo; Choi, Michael Y.

    2012-09-21

    Highlights: Black-Right-Pointing-Pointer Embryonic stem cells (ESCs) lacking non-canonical miRNAs proliferate slower. Black-Right-Pointing-Pointer miR-320 and miR-702 are two non-canonical miRNAs expressed in ESCs. Black-Right-Pointing-Pointer miR-320 and miR-702 promote proliferation of Dgcr8-deficient ESCs. Black-Right-Pointing-Pointer miR-320 targets p57 and helps to release Dgcr8-deficient ESCs from G1 arrest. Black-Right-Pointing-Pointer miR-702 targets p21 and helps to release Dgcr8-deficient ESCs from G1 arrest. -- Abstract: MicroRNAs are known to contribute significantly to stem cell phenotype by post-transcriptionally regulating gene expression. Most of our knowledge of microRNAs comes from the study of canonical microRNAs that require two sequential cleavages by the Drosha/Dgcr8 heterodimer and Dicer to generate mature products. In contrast, non-canonical microRNAs bypass the cleavage by the Drosha/Dgcr8 heterodimer within the nucleus but still require cytoplasmic cleavage by Dicer. The function of non-canonical microRNAs in embryonic stem cells (ESCs) remains obscure. It has been hypothesized that non-canonical microRNAs have important roles in ESCs based upon the phenotypes of ESC lines that lack these specific classes of microRNAs; Dicer-deficient ESCs lacking both canonical and non-canonical microRNAs have much more severe proliferation defect than Dgcr8-deficient ESCs lacking only canonical microRNAs. Using these cell lines, we identified two non-canonical microRNAs, miR-320 and miR-702, that promote proliferation of Dgcr8-deficient ESCs by releasing them from G1 arrest. This is accomplished by targeting the 3 Prime -untranslated regions of the cell cycle inhibitors p57 and p21 and thereby inhibiting their expression. This is the first report of the crucial role of non-canonical microRNAs in ESCs.

  10. miR-182 targets CHL1 and controls tumor growth and invasion in papillary thyroid carcinoma

    SciTech Connect

    Zhu, Hongling; Fang, Jin; Zhang, Jichen; Zhao, Zefei; Liu, Lianyong; Wang, Jingnan; Xi, Qian; Gu, Mingjun

    2014-07-18

    Highlights: miR-182 and CHL1 expression patterns are negatively correlated. CHL1 is a direct target of miR-182 in PTC cells. miR-182 suppression inhibits PTC cell growth and invasion. CHL1 is involved in miR-182-mediated cell behavior. - Abstract: In this study, we investigated the role and underlying mechanism of action of miR-182 in papillary thyroid carcinoma (PTC). Bioinformatics analysis revealed close homolog of LI (CHL1) as a potential target of miR-182. Upregulation of miR-182 was significantly correlated with CHL1 downregulation in human PTC tissues and cell lines. miR-182 suppressed the expression of CHL1 mRNA through direct targeting of the 3?-untranslated region (3?-UTR). Downregulation of miR-182 suppressed growth and invasion of PTC cells. Silencing of CHL1 counteracted the effects of miR-182 suppression, while its overexpression mimicked these effects. Our data collectively indicate that miR-182 in PTC promotes cell proliferation and invasion through direct suppression of CHL1, supporting the potential utility of miR-182 inhibition as a novel therapeutic strategy against PTC.

  11. Downregulation of miRNA-30c and miR-203a is associated with hepatitis C virus core protein-induced epithelial–mesenchymal transition in normal hepatocytes and hepatocellular carcinoma cells

    SciTech Connect

    Liu, Dongjing; Wu, Jilin; Liu, Meizhou; Yin, Hui; He, Jiantai; Zhang, Bo

    2015-09-04

    Hepatitis C virus (HCV) Core protein has been demonstrated to induce epithelial–mesenchymal transition (EMT) and is associated with cancer progression of hepatocellular carcinoma (HCC). However, how the Core protein regulates EMT is still unclear. In this study, HCV Core protein was overexpressed by an adenovirus. The protein levels of EMT markers were measured by Western blot. The xenograft animal model was established by inoculation of HepG2 cells. Results showed that ectopic expression of HCV core protein induced EMT in L02 hepatocytes and HepG2 tumor cells by upregulating vimentin, Sanl1, and Snal2 expression and downregulating E-cadherin expression. Moreover, Core protein downregulated miR-30c and miR-203a levels in L02 and HepG2 cells, but artificial expression of miR-30c and miR-203a reversed Core protein-induced EMT. Further analysis showed that ectopic expression of HCV core protein stimulated cell proliferation, inhibited apoptosis, and increased cell migration, whereas artificial expression of miR-30c and miR-203a significantly reversed the role of Core protein in these cell functions in L02 and HepG2 cells. In the HepG2 xenograft tumor models, artificial expression of miR-30c and miR-203a inhibited EMT and tumor growth. Moreover, L02 cells overexpressing Core protein can form tumors in nude mice. In HCC patients, HCV infection significantly shortened patients' survival time, and loss of miR-30c and miR-203 expression correlated with poor survival. In conclusion, HCV core protein downregulates miR-30c and miR-203a expression, which results in activation of EMT in normal hepatocytes and HCC tumor cells. The Core protein-activated-EMT is involved in the carcinogenesis and progression of HCC. Loss of miR-30c and miR-203a expression is a marker for the poor prognosis of HCC. - Highlights: • HCV core protein downregulates miR-30c and miR-203a expression. • Downregulation of miR-30c and miR-203a activates EMT. • Activated-EMT is involved in the

  12. FLINT HILLS RESOURES, LP- FE DKT NO. 15-169-LNG- APPLICATION FOR SHORT-TERM, MULTI-CONTRACT AUTHORIZATION TO EXPORT LNG TO FTANs

    Energy.gov [DOE]

    The Office of Fossil Energy gives notice of receipt of an application filed on November 5, 2015, by Flint Hills Resources,  LP requesting  long-term, multi-contract authorization to export up to 12...

  13. Voluntary Protection Program Onsite Review, CH2M HILL B&W West Valley LLC, West Valley Demonstration Project – October 2013

    Energy.gov [DOE]

    Evaluation to determine whether CH2M HILL B&W West Valley LLC, West Valley Demonstration Project is performing at a level deserving DOE-VPP Star recognition.

  14. U.S. Department of Energy, Richland Operations Office And CH2M HILL Plateau Remediation Company Partnering Charter For Partnering Performance Agreement

    Energy.gov [DOE]

    The U.S. Department of Energy (DOE) Richland Operations Office (DOE-RL) and CH2M HILL Plateau Remediation Company (CHPRC) are committed to continuous improvement and will utilize principles of the...

  15. miR-128 and its target genes in tumorigenesis and metastasis

    SciTech Connect

    Li, Molin, E-mail: molin_li@hotmail.com [Dalian Medical University, Dalian 116044 (China); Fu, Weiming [Center for Food Safety and Environmental Technology, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458 (China); Wo, Lulu; Shu, Xiaohong [Dalian Medical University, Dalian 116044 (China); Liu, Fang [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China); Li, Chuangang, E-mail: li_chuangang@sina.com [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China)

    2013-12-10

    MicroRNAs (miRNAs) are a class of endogenous, non-coding, 1824 nucleotide length single-strand RNAs that could modulate gene expression at post-transcriptional level. Previous studies have shown that miR-128 enriched in the brain plays an important role in the development of nervous system and the maintenance of normal physical functions. Aberrant expression of miR-128 has been detected in many types of human tumors and its validated target genes are involved in cancer-related biological processes such as cell proliferation, differentiation and apoptosis. In this review, we will summarize the roles of miR-128 and its target genes in tumorigenesis and metastasis. - Highlights: Aberrant expression of miR-128 can be observed in many kinds of malignant tumors. The molecular mechanisms regulating miR-128 expression are elucidated. Roles of miR-128 and its target genes in tumorigenesis and metastasis are summarized.

  16. miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

    SciTech Connect

    Mizuno, Yosuke; Yagi, Ken; Tokuzawa, Yoshimi; Kanesaki-Yatsuka, Yukiko; Suda, Tatsuo; Katagiri, Takenobu; Fukuda, Toru; Maruyama, Masayoshi; Okuda, Akihiko; Amemiya, Tomoyuki; Kondoh, Yasumitsu; Tashiro, Hideo; Okazaki, Yasushi

    2008-04-04

    Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

  17. Air quality monitoring for dioxins, furans and PCBs in the Swan Hills area, Summer 1997, July 7 to August 1

    SciTech Connect

    1997-12-31

    Summarizes results of air quality monitoring activities carried out in the Swan Hills area of Alberta in summer 1997. At four locations in the area, samples of dioxin, furan, and polychlorinated biphenyls were analyzed and ambient concentrations determined. Results are presented in terms of toxic equivalents of dioxins and furans, total dioxins, total furans, and total polychlorinated biphenyls, normalized by compounds within each homologue group.

  18. Fracture-coating minerals in the Topopah Spring Member and upper tuff of Calico Hills from drill hole J-13

    SciTech Connect

    Carlos, B.

    1989-02-01

    Fracture-lining minerals from drill core in the Topopah Spring Member of the Paintbrush Tuff and the tuff of Calico Hills from water well J-13 were studied to identify the differences between these minerals and those seen in drill core USW G-4. In USW G-4 the static water level (SWL) occurs below the tuff of Calico Hills, but in J-13 the water table is fairly high in the Topopah Spring Member. There are some significant differences in fracture minerals between these two holes. In USW G-4 mordenite is a common fracture-lining mineral in the Topopah Spring Member, increasing in abundance with depth. Euhedral heulandite >0.1 mm in length occurs in fractures for about 20 m above the lower vitrophyre. In J-13, where the same stratigraphic intervals are below the water table, mordenite is uncommon and euhedral heulandite is not seen. The most abundant fracture coating in the Topopah Spring Member in J-13 is drusy quartz, which is totally absent in this interval in USW G-4. Though similar in appearance, the coatings in the vitrophyre have different mineralogy in the two holes. In USW G-4 the coatings are extremely fine grained heulandite and smectite. In J-13 the coatings are fine-grained heulandite, chabazite, and alkali feldspar. Chabazite has not been identified from any other hole in the Yucca Mountain area. Fractures in the tuff of Calico Hills have similar coatings in core from both holes. In J-13, as in USW G-4, the tuff matrix of the Topopah Spring Member is welded and devitrified and that of the tuff of Calico Hills is zeolitic. 11 refs., 10 figs., 5 tabs.

  19. Summary of Degas II performance at the US Strategic Petroleum Reserve Big Hill site.

    SciTech Connect

    Rudeen, David K.; Lord, David L.

    2007-10-01

    Crude oil stored at the US Strategic Petroleum Reserve (SPR) requires mitigation procedures to maintain oil vapor pressure within program delivery standards. Crude oil degasification is one effective method for lowering crude oil vapor pressure, and was implemented at the Big Hill SPR site from 2004-2006. Performance monitoring during and after degasification revealed a range of outcomes for caverns that had similar inventory and geometry. This report analyzed data from SPR degasification and developed a simple degas mixing (SDM) model to assist in the analysis. Cavern-scale oil mixing during degassing and existing oil heterogeneity in the caverns were identified as likely causes for the range of behaviors seen. Apparent cavern mixing patterns ranged from near complete mixing to near plug flow, with more mixing leading to less efficient degassing due to degassed oil re-entering the plant before 100% of the cavern oil volume was processed. The report suggests that the new cavern bubble point and vapor pressure regain rate after degassing be based on direct in-cavern measurements after degassing as opposed to using the plant outlet stream properties as a starting point, which understates starting bubble point and overstates vapor pressure regain. Several means to estimate the cavern bubble point after degas in the absence of direct measurement are presented and discussed.

  20. Geomechanical analysis to predict the oil leak at the wellbores in Big Hill Strategic Petroleum Reserve

    SciTech Connect

    Park, Byoung Yoon

    2014-02-01

    Oil leaks were found in wellbores of Caverns 105 and 109 at the Big Hill Strategic Petroleum Reserve site. According to the field observations, two instances of casing damage occurred at the depth of the interbed between the caprock bottom and salt top. A three dimensional finite element model, which contains wellbore element blocks and allows each cavern to be configured individually, is constructed to investigate the wellbore damage mechanism. The model also contains element blocks to represent interface between each lithology and a shear zone to examine the interbed behavior in a realistic manner. The causes of the damaged casing segments are a result of vertical and horizontal movements of the interbed between the caprock and salt dome. The salt top subsides because the volume of caverns below the salt top decrease with time due to salt creep closure, while the caprock subsides at a slower rate because the caprock is thick and stiffer. This discrepancy yields a deformation of the well. The deformed wellbore may fail at some time. An oil leak occurs when the wellbore fails. A possible oil leak date of each well is determined using the equivalent plastic strain failure criterion. A well grading system for a remediation plan is developed based on the predicted leak dates of each wellbore.

  1. Silica phase changes: Diagenetic agent for oil entrapment, Lost Hills field, California

    SciTech Connect

    Julander, D.R.; Szymanski, D.L. )

    1991-02-01

    The siliceous shales of the Monterey Group are the primary development target at Lost Hills. Silica phase changes have influenced the distribution and entrapment of hydrocarbons. With increasing temperature, opal A phase diatomite is converted to opal CT and finally quartz phase rock. All phases are low in permeability. The opal A diatomite is characteristically high in oil saturation and productive saturation. Productivity from this phase is dependent on structural position and fieldwide variations in oil viscosity and biodegradation. The deeper chert reservoir coincides with the opal CT to quartz phase transition. Porosity is again reduced in this transition, but saturations in the quartz phase rocks increase. Tests in the chert reservoir indicate a single, low-permeability system, suggesting the importance of matric contribution. resistivity and porosity in the diatomite, and resistivity and velocity in the chert, are the physical properties which best reflect saturation. Methods exploiting these properties (FMS, BHTV, borehole, and surface shear wave studies) should be helpful in further characterizing the reservoirs and identifying future pay.

  2. Drilling fluids and lost circulation in hot dry rock geothermal wells at Fenton Hill

    SciTech Connect

    Nuckols, E.B.; Miles, D.; Laney, R.; Polk, G.; Friddle, H.; Simpson, G.; Baroid, N.L.

    1981-01-01

    Geothermal hot dry rock drilling activities at Fenton Hill in the Jemez Mountains of northern New Mexico encountered problems in designing drilling fluids that will reduce catastrophic lost circulation. Four wells (GT-2, EE-1, EE-2, and EE-3) penetrated 733 m (2405 ft) of Cenozoic and Paleozoic sediments and Precambrian crystalline rock units to +4572 m (+15,000 ft). The Cenozoic rocks consist of volcanics (rhyolite, tuff, and pumice) and volcaniclastic sediments. Paleozoic strata include Permian red beds (Abo Formation) and the Pennsylvanian Madera and Sandia Formations, which consist of massive limestones and shales. Beneath the Sandia Formation are igneous and metamorphic rocks of Precambrian age. The drilling fluid used for the upper sedimentary formations was a polymeric flocculated bentonite drilling fluid. Severe loss of circulation occurred in the cavernous portions of the Sandia limestones. The resultant loss of hydrostatic head caused sloughing of the Abo and of some beds within the Madera Formation. Stuck pipe, repetitive reaming, poor casing cement jobs and costly damage to the intermediate casing resulted. The Precambrian crystalline portion of the EE-2 and EE-3 wells were directionally drilled at a high angle, and drilled with water as the primary circulating fluid. Due to high temperatures (approximately 320/sup 0/C (608/sup 0/F) BHT) and extreme abrasiveness of the deeper part of the Precambrian crystalline rocks, special problems of corrosion inhibition and of torque friction were incurred.

  3. Experimental plan and construction guidance for Hanford Protective Barrier Test at Hill AFB, Utah

    SciTech Connect

    Kirkham, R.R.; Gee, G.W.

    1994-04-01

    Permanent isolation surface barriers are needed to fully isolate buried waste materials for long periods of time from the general environment. Multi-layer surface barriers that use natural earthen materials overlying bio-intrusion layers of rock and/or asphalt are expected to reduce infiltration or deep percolation to less than 0.5 mm (0.02 in.) of water. The layering also reduces root penetration, preventing uptake and translocation of hazardous materials to the surface vegetation. The opportunity exists to test the Hanford Barrier in a wetter, colder climate at an existing lysimeter facility located at Hill Air Force Base near Salt Lake, Utah. The use of an existing lysimeter facility simplifies the construction effort and allows comparison of the Hanford Protective Barrier with an existing US Environmental Protection Agency-Resource Conservation and Recovery Act clay cap already undergoing tests at the lysimeter site. This document presents the experimental plan for testing the Hanford Protective Barrier in a wetter, colder climate.

  4. A theoretical model of subsidence caused by petroleum production: Big Hill Field, Jefferson County, Texas

    SciTech Connect

    Hill, D.W.; Sharp, J.M. Jr. . Dept. of Geological Sciences)

    1993-02-01

    In the Texas Gulf Coastal Plain, there is a history of oil and gas production extending over 2 to 5 decades. Concurrent with this production history, there has been unprecedented population growth accompanied by vastly increased groundwater demands. Land subsidence on both local and regional bases in this geologic province has been measured and predicted in several studies. The vast majority of these studies have addressed the problem from the standpoint of groundwater usage while only a few have considered the effects of oil and gas production. Based upon field-based computational techniques (Helm, 1984), a model has been developed to predict land subsidence caused by oil and gas production. This method is applied to the Big Hill Field in Jefferson County, Texas. Inputs include production data from a series of wells in this field and lithologic data from electric logs of these same wells. Outputs include predicted amounts of subsidence, the time frame of subsidence, and sensitivity analyses of compressibility and hydraulic conductivity estimates. Depending upon estimated compressibility, subsidence, to date, is predicted to be as high as 20 cm. Similarly, depending upon estimated vertical hydraulic conductivity, the time frame may be decades for this subsidence. These same methods can be applied to other oil/gas fields with established production histories as well as new fields when production scenarios are assumed. Where subsidence has been carefully measured above petroleum reservoir, the model may be used inversely to calculate sediment compressibilities.

  5. Observations of wake characteristics at the Goodnoe Hills MOD-2 array

    SciTech Connect

    Buck, J.W.; Renne, D.S.

    1985-08-01

    The array of three MOD-2 wind turbines and two meteorological towers at Goodnoe Hills, Washington, provides an opportunity to evaluate turbine and wake interactions in a real environment. The triangular arrangement of the three turbines provides opportunities to study the effect of wakes on the performance of a downwind turbine at three different distances: 5, 7, and 10 rotor diameters (D), where 1 D is 300 ft. The information obtained from this test configuration is critical to future wind farm activities and is a key objective of the MOD-2 test program. This report describes the analysis of data measured at the turbines and towers from August 29 to November 12, 1982. The data are 2-min averages of 1-s values. Background flow characteristics were also examined to determine if flow variations across the site could mask wake measurements. For this analysis, one year's data gathered at the meteorological towers were analyzed. The results show some differences between characteristics measured at the towers, but these differences were not great enough to consider in evaluating wakes at the towers.

  6. Implications from a study of the timing of oil entrapment in Monterey siliceous shales, Lost Hills, San Joaquin Valley, California

    SciTech Connect

    Julander, D.R. )

    1992-01-01

    The oil and gas-rich upper Miocene siliceous shales of the Monterey Group are the primary development target in the Lost Hills Oil Field, San Joaquin Valley, California. As a result of diagenesis, the siliceous shales can be subdivided by opal phase into three sections (from shallow to deep): the Opal-A diatomites which are rich in oil saturation; the Opal-CT porcellanites which are predominantly wet but include pockets of moderate oil saturation; and the Quartz cherts and porcellanites which in some places are highly oil saturated immediately below the Opal CT section. Productivity trends in each of the three sections have been established through drilling and production testing, but a predictive model was not available until a study of the timing of oil entrapment at Lost Hills was recently completed. The study included an analysis of the depositional history of the siliceous shales and timing of: (1) structural growth of the Lost Hills fold, (2) source-rock maturation, and (3) development of the opal-phase segregation of the Monterey shales. The study led to enhanced understanding of the known oil saturation and production trends in the three opal-phase sections and yielded a predictive model that is being used to identify areas in the field with remedial or delineation potential. The study also produced evidence of fold axis rotation during the Pliocene and Pleistocene that helps explain differences in fracture orientations within the Monterey shales.

  7. Extremely organic-rich coma of comet C/2010 G2 (Hill) during its outburst in 2012

    SciTech Connect

    Kawakita, Hideyo; Kobayashi, Hitomi; Russo, Neil Dello; Vervack, Ron Jr.; Weaver, Harold A.; DiSanti, Mike A.; Opitom, Cyrielle; Jehin, Emmanuel; Manfroid, Jean; Gillon, Michael; Cochran, Anita L.; Harris, Walter M.; Bockelée-Morvan, Dominique; Biver, Nicolas; Crovisier, Jacques; McKay, Adam J.

    2014-06-20

    We performed high-dispersion near-infrared spectroscopic observations of comet C/2010 G2 (Hill) at 2.5 AU from the Sun using NIRSPEC (R ≈ 25,000) at the Keck II Telescope on UT 2012 January 9 and 10, about a week after an outburst had occurred. Over the two nights of our observations, prominent emission lines of CH{sub 4} and C{sub 2}H{sub 6}, along with weaker emission lines of H{sub 2}O, HCN, CH{sub 3}OH, and CO were detected. The gas production rate of CO was comparable to that of H{sub 2}O during the outburst. The mixing ratios of CO, HCN, CH{sub 4}, C{sub 2}H{sub 6}, and CH{sub 3}OH with respect to H{sub 2}O were higher than those for normal comets by a factor of five or more. The enrichment of CO and CH{sub 4} in comet Hill suggests that the sublimation of these hypervolatiles sustained the outburst of the comet. Some fraction of water in the inner coma might exist as icy grains that were likely ejected from nucleus by the sublimation of hypervolatiles. Mixing ratios of volatiles in comet Hill are indicative of the interstellar heritage without significant alteration in the solar nebula.

  8. miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer

    Office of Scientific and Technical Information (OSTI)

    Cells by Targeting the TLR1/NF-κB Signaling Pathway (Journal Article) | SciTech Connect miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer Cells by Targeting the TLR1/NF-κB Signaling Pathway Citation Details In-Document Search Title: miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer Cells by Targeting the TLR1/NF-κB Signaling Pathway Purpose: Many miRNAs have been identified as essential issues and core determining factors in tumor radiation.

  9. Overexpression of miR-206 suppresses glycolysis, proliferation and migration in breast cancer cells via PFKFB3 targeting

    SciTech Connect

    Ge, Xin; Lyu, Pengwei; Cao, Zhang; Li, Jingruo; Guo, Guangcheng; Xia, Wanjun; Gu, Yuanting

    2015-08-07

    miRNAs, sorting as non-coding RNAs, are differentially expressed in breast tumor and act as tumor promoters or suppressors. miR-206 could suppress the progression of breast cancer, the mechanism of which remains unclear. The study here was aimed to investigate the effect of miR-206 on human breast cancers. We found that miR-206 was down-regulated while one of its predicted targets, 6-Phosphofructo-2-kinase (PFKFB3) was up-regulated in human breast carcinomas. 17β-estradiol dose-dependently decreased miR-206 expression as well as enhanced PFKFB3 mRNA and protein expression in estrogen receptor α (ERα) positive breast cancer cells. Furthermore, we identified that miR-206 directly interacted with 3′-untranslated region (UTR) of PFKFB3 mRNA. miR-206 modulated PFKFB3 expression in MCF-7, T47D and SUM159 cells, which was influenced by 17β-estradiol depending on ERα expression. In addition, miR-206 overexpression impeded fructose-2,6-bisphosphate (F2,6BP) production, diminished lactate generation and reduced cell proliferation and migration in breast cancer cells. In conclusion, our study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration. - Highlights: • miR-206 was down-regulated and PFKFB3 was up-regulated in human breast carcinomas. • 17β-estradiol regulated miR-206 and PFKFB3 expression in ERα+ cancer cells. • miR-206directly interacted with 3′-UTR of PFKFB3 mRNA. • miR-206 fructose-2,6-bisphosphate (F2,6BP) impeded production and lactate generation. • miR-206 reduced cell proliferation and migration in breast cancer cells.

  10. AMENDMENT OF SOLICITATION/MODIFICATlON OF CONTRACT MI54 I See...

    National Nuclear Security Administration (NNSA)

    MI54 I See Block 16C I REQ. NO. Babcock & Wilcox Technical Services Pantex, LLC PO Box 30020 Amarillo, TX 79120 2. AMENDMENTIMODIFICATION NO. 1 3. EFFECTIVE DATE 1 4. ...

  11. File:USDA-CE-Production-GIFmaps-MI.pdf | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    MI.pdf Jump to: navigation, search File File history File usage Michigan Ethanol Plant Locations Size of this preview: 463 599 pixels. Other resolution: 464 600 pixels. Full...

  12. Simulations of neutron multiplicity measurements with MCNP-PoliMi.

    SciTech Connect

    Mattingly, John K.; Pozzi, Sara A.; Clarke, Shaun D.; Dennis, Ben D.; Miller, Eric C.

    2010-09-01

    The heightened focus on nuclear safeguards and accountability has increased the need to develop and verify simulation tools for modeling these applications. The ability to accurately simulate safeguards techniques, such as neutron multiplicity counting, aids in the design and development of future systems. This work focuses on validating the ability of the Monte Carlo code MCNPX-PoliMi to reproduce measured neutron multiplicity results for a highly multiplicative sample. The benchmark experiment for this validation consists of a 4.5-kg sphere of plutonium metal that was moderated by various thicknesses of polyethylene. The detector system was the nPod, which contains a bank of 15 3He detectors. Simulations of the experiments were compared to the actual measurements and several sources of potential bias in the simulation were evaluated. The analysis included the effects of detector dead time, source-detector distance, density, and adjustments made to the value of {nu}-bar in the data libraries. Based on this analysis it was observed that a 1.14% decrease in the evaluated value of {nu}-bar for 239Pu in the ENDF-VII library substantially improved the accuracy of the simulation.

  13. Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Sault Ste. Marie Tribe of Chippewa Indians, MI Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI The Sault Ste. Marie Tribe of Chippewa Indians is a 44,000-strong federally recognized Indian tribe that is an economic, social and cultural force in its community across the eastern Upper Peninsula counties of Chippewa, Luce, Mackinac, Schoolcraft, Alger, Delta and Marquette, with housing and tribal centers, casinos, and other enterprises that employ

  14. Secretary Bodman Makes "Energizing America for Energy Security" Tour Stops

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    to Launch New Solar Facility | Department of Energy Makes "Energizing America for Energy Security" Tour Stops to Launch New Solar Facility Secretary Bodman Makes "Energizing America for Energy Security" Tour Stops to Launch New Solar Facility July 14, 2005 - 2:10pm Addthis AUBURN HILLS, MICH - Secretary of Energy Samuel W. Bodman toured the world's largest amorphous silicon thin-fill solar manufacturing plant in Auburn Hills, Mich., today, and joined United Solar Ovonic

  15. Reservoir compartmentalization caused by mass transport deposition Northwest Stevens pool, Elk Hills Naval Petroleum Reserves, California

    SciTech Connect

    Milliken, M.D.; McJannet, G.S.; Shiflett, D.W.; Deutsch, H.A.

    1996-12-31

    The {open_quotes}A{close_quotes} sands of the Northwest Stevens Pool consist of six major subdivisions (A1-A6) and numerous sublayers. These sands are above the {open_quotes}N Point{close_quotes} stratigraphic marker, making them much younger than most other Stevens sands at Elk Hills. Cores show the A1-A3 sands to be possibly mass transport deposition, primarily debris flows, slumps, and sand injection bodies. The A4-A6 sands are characterized by normally graded sheet-like sand bodies Hospital of traditional outer fan turbidite lithofacies. Most current production from the A1-A2 interval comes from well 373A-7R, are completed waterflood wells that came on line in 1992 at 1400 BOPD. Well 373A-7R is an anomaly in the A1-A2 zone, where average production from the other ten wells is 200 BOPD. Other evidence for compartmentalization in the A1-A2 interval includes sporadic oil-water contacts and drawdown pressures, difficult log correlations, and rapid thickness changes. In 1973, well 362-7R penetrated 220 ft of wet Al sand. The well was redrilled updip and successfully completed in the A1, where the oil-water contact is more than 130 ft lower than the original hole and faulting is not apparent. In 1992, horizontal well 323H-7R unexpectedly encountered an entirely wet Al wedge zone. Reevaluation of the A1-A3 and other sands as mass transport origin is important for modeling initialization and production/development strategies.

  16. Reservoir compartmentalization caused by mass transport deposition Northwest Stevens pool, Elk Hills Naval Petroleum Reserves, California

    SciTech Connect

    Milliken, M.D.; McJannet, G.S. ); Shiflett, D.W. ); Deutsch, H.A. )

    1996-01-01

    The [open quotes]A[close quotes] sands of the Northwest Stevens Pool consist of six major subdivisions (A1-A6) and numerous sublayers. These sands are above the [open quotes]N Point[close quotes] stratigraphic marker, making them much younger than most other Stevens sands at Elk Hills. Cores show the A1-A3 sands to be possibly mass transport deposition, primarily debris flows, slumps, and sand injection bodies. The A4-A6 sands are characterized by normally graded sheet-like sand bodies Hospital of traditional outer fan turbidite lithofacies. Most current production from the A1-A2 interval comes from well 373A-7R, are completed waterflood wells that came on line in 1992 at 1400 BOPD. Well 373A-7R is an anomaly in the A1-A2 zone, where average production from the other ten wells is 200 BOPD. Other evidence for compartmentalization in the A1-A2 interval includes sporadic oil-water contacts and drawdown pressures, difficult log correlations, and rapid thickness changes. In 1973, well 362-7R penetrated 220 ft of wet Al sand. The well was redrilled updip and successfully completed in the A1, where the oil-water contact is more than 130 ft lower than the original hole and faulting is not apparent. In 1992, horizontal well 323H-7R unexpectedly encountered an entirely wet Al wedge zone. Reevaluation of the A1-A3 and other sands as mass transport origin is important for modeling initialization and production/development strategies.

  17. Department of Justice: CH2M Hill Hanford Group Inc. Admits Criminal Conduct, Parent Company Agrees to Cooperate in Ongoing Investigation and Pay $18.5 Million to Resolve Civil and Criminal Allegations

    Energy.gov [DOE]

    The Justice Department, in conjunction with the U.S. Attorney’s Office for the Eastern District of Washington, announced today that Colorado-based CH2M Hill Hanford Group Inc. (CHG) and its parent company, CH2M Hill Companies Ltd. (CH2M Hill) have agreed that CHG committed federal criminal violations, defrauding the public by engaging in years of widespread time card fraud.

  18. miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells

    SciTech Connect

    Zhang, Jie; Zheng, Fangxia; Yu, Gang; Yin, Yanhua; Lu, Qingyang

    2013-11-01

    Highlights: miR-196a was overexpressed in cervical cancer tissue compared to normal tissue. miR-196a expression elevated proliferation and migration of cervical cancer cells. miR-196a inhibited NTN4 expression by binding 3?-UTR region of NTN4 mRNA. NTN4 inversely correlated with miR-196a expression in cervical tissue and cell line. NTN4 expression was low in cervical cancer tissue compared to normal tissue. -- Abstract: Recent research has uncovered tumor-suppressive and oncogenic potential of miR-196a in various tumors. However, the expression and mechanism of its function in cervical cancer remains unclear. In this study, we assess relative expression of miR-196a in cervical premalignant lesions, cervical cancer tissues, and four cancer cell lines using quantitative real-time PCR. CaSki and HeLa cells were treated with miR-196a inhibitors, mimics, or pCDNA/miR-196a to investigate the role of miR-196a in cancer cell proliferation and migration. We demonstrated that miR-196a was overexpressed in cervical intraepithelial neoplasia 23 and cervical cancer tissue. Moreover, its expression contributes to the proliferation and migration of cervical cancer cells, whereas inhibiting its expression led to a reduction in proliferation and migration. Five candidate targets of miR-196a chosen by computational prediction and Cervical Cancer Gene Database search were measured for their mRNA in both miR-196a-overexpressing and -depleted cancer cells. Only netrin 4 (NTN4) expression displayed an inverse association with miR-196a. Fluorescent reporter assays revealed that miR-196a inhibited NTN4 expression by targeting one binding site in the 3?-untranslated region (3?-UTR) of NTN4 mRNA. Furthermore, qPCR and Western blot assays verified NTN4 expression was downregulated in cervical cancer tissues compared to normal controls, and in vivo mRNA level of NTN4 inversely correlated with miR-196a expression. In summary, our findings provide new insights about the functional role of

  19. Mesozoic and Cenozoic structural geology of the CP Hills, Nevada Test Site, Nye County, Nevada; and regional implications

    SciTech Connect

    Caskey, S.J.

    1991-08-01

    Detailed mapping and structural analysis of upper Proterozoic and Paleozoic rocks in the CP Hills of the Nevada Test Site, together with analysis of published maps and cross sections and a reconnaissance of regional structural relations indicate that the CP thrust of Barnes and Poole (1968) actually comprises two separate, oppositely verging Mesozoic thrust systems: (1) the west-vergent CP thrust which is well exposed in the CP Hills and at Mine Mountain, and (2) the east-vergent Belted Range thrust located northwest of Yucca Flat. West-vergence of the CP thrust is indicated by large scale west-vergent recumbent folds in both its hangingwall and footwall and by the fact that the CP thrust ramps up section through hangingwall strata toward the northwest. Regional structural relations indicate that the CP thrust forms part of a narrow sigmoidal belt of west-vergent folding and thrusting traceable for over 180 km along strike. The Belted Range thrust represents earlier Mesozoic deformation that was probably related to the Last Chance thrust system in southeastern California, as suggested by earlier workers. A pre-Tertiary reconstruction of the Cordilleran fold and thrust belt in the region between the NTS and the Las Vegas Range bears a close resemblance to other regions of the Cordillera and has important implications for the development of hinterland-vergent deformation as well as for the probable magnitude of Tertiary extension north of Las Vegas Valley. Subsequent to Mesozoic deformation, the CP Hills were disrupted by at least two episodes of Tertiary extensional deformation: (1) an earlier episode represented by pre-middle Miocene low-angle normal faults, and (2) a later, post-11 Ma episode of high-angle normal faulting. Both episodes of extension were related to regional deformation, the latter of which has resulted in the present basin and range topography of the NTS region.

  20. Reservoir analysis study, Naval Petroleum Reserve No. 1, Elk Hills Field, Kern County, California: Phase 2 report, Executive summary

    SciTech Connect

    Not Available

    1988-07-01

    The Naval Petroleum Reserve No. 1 (Elk Hills) is located in Kern County, California, and is jointly owned by the US Department of Energy and Chevron USA Inc. The Elk Hills Field is presently producing oil and gas from five geologic zones. These zones contain a number of separate and geologically complex reservoirs. Considerable field development and production of oil and gas have occurred since initial estimates of reserves were made. Total cumulative field production through December 1987 is 850 MMBbls of oil, 1.2 Tcf of gas and 648.2 MMBbls of water. In December 1987, field producing rates expressed on a calendar day basis amounted to 110,364 BOPD, 350,946 Mcfd and 230,179 BWPD from 1157 producers. In addition, a total of two reservoirs have gas injection in progress and four reservoirs have water injection in progress and four reservoirs have water injection in progress. Cumulative gas and water injection amounted to 586 Bcf of gas and 330 MMB of water. December 1987 gas and water injection rates amounted to 174 MMcfd and 234 MBWPD, into 129 injectors. In addition, a steamflood pilot program is currently active in the Eastern Shallow Oil Zone. Jerry R. Bergeson and Associates, Inc. (Bergeson) has completed Phase II of the Reservoir Analysis, Naval Petroleum Reserve Number 1, Elk Hills Oilfield, California. The objectives for this phase of the study included the establishment of revised estimates of the original oil and gas-in-place for each of the zones/reservoirs, estimation of the remaining proved developed, proved undeveloped, probable and possible reserves, and assessment of the effects of historical development and production operations and practices on recoverable reserves. 28 figs., 37 tabs.

  1. A validation test for Adagio through replication of Big Hill and Bayou Choctaw JAS3D models.

    SciTech Connect

    Park, Byoung Yoon

    2013-06-01

    JAS3D, a three dimensional iterative solid mechanics code, has been used for structural analyses for the Strategic Petroleum Reserve system since the 1990s. JAS3D is no longer supported by Sandia National Laboratories, and has been replaced by Adagio. To validate the transition from JAS3D to Adagio, the existing JAS3D input decks and user subroutines for Bayou Choctaw and Big Hill models were converted for use with Adagio. The calculation results from the Adagio runs are compared to the JAS3D. Since the Adagio results are very similar to the JAS3D results, Adagio is judged to be performing satisfactorily.

  2. A CO{sub 2}-based analysis of a light-oil steamflood at NPR-1, Elk Hills, CA

    SciTech Connect

    Shotts, D.R.; Senum, G.I.

    1992-12-01

    A steamdrive pilot was run on a light-oil reservoir at the Naval Petroleum Reserve No 1 (NPR1) in the Elk Hills oil field, Kern County, CA. The goal of this work was to establish a correlation between a documented growth in CO{sub 2} concentrations found in producing wells in the pilot area to the light-oil steamflood (LOSF); then to use a thermodynamic analysis of the expended energy to come up with an energy efficiency of the steamdrive.

  3. Chemical migration by contact metamorphism between pegmatite/country rocks: natural analogs for radionuclides migration. [Black Hills, South Dakota

    SciTech Connect

    Laul, J.C.; Walker, R.J.; Shearer, C.K.; Papike, J.J.; Simon, S.B.

    1983-10-01

    Comparison of trace element signatures of country rocks as a function of distance from the contact with two pegmatites, Tin Mountain and Etta, in the Black Hills of South Dakota, suggests that some elements such as K, Li, Rb, Cs, As, Sb, Zn and Pb, have migrated to distances of 4 to 40 meters during contact metamorphism. The relative degree of migration varies depending on the element. On the other hand, there is virtually no migration of rare earth elements (REE), Al, Sc, Cr, Hf, U, and Th. Biotite and muscovite are effective trace element traps for Li, Rb and Cs. Biotite has a greater affinity for Rb, Cs and Li than muscovite.

  4. HIA 2015 DOE Zero Energy Ready Home Case Study: Mandalay Homes, Vision Hill Lot 1, Glendale, AZ

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Homes Vision Hill Lot 1 Glendale, AZ DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced technologies are designed in to

  5. <mi>B> -meson decay constants from 2+1 -flavor lattice QCD with domain-wall light quarks and relativistic heavy quarks

    SciTech Connect

    Christ, N. H.; Flynn, J. M.; Izubuchi, T.; Kawanai, T.; Lehner, C.; Soni, A.; Van de Water, R. S.; Witzel, O.

    2015-03-01

    We calculate the mi>Bmi>-meson decay constants mi>fmi>mi>Bmi>, mi>fmi>mi>Bmi>mi>smi>, and their ratio in unquenched lattice QCD using domain-wall light quarks and relativistic mi>b> quarks. We use gauge-field ensembles generated by the RBC and UKQCD collaborations using the domain-wall fermion action and Iwasaki gauge action with three flavors of light dynamical quarks. We analyze data at two lattice spacings of mi>ami>0.11, 0.086 fm with unitary pion masses as light as mi>Mmi>mi>πmi>290mi>MeV>; this enables us to control the extrapolation to the physical light-quark masses and continuum. For the mi>b> quarks we use the anisotropic clover action with the relativistic heavy-quark interpretation, such that discretization errors from the heavy-quark action are of the same size as from the light-quark sector. We renormalize the

  6. miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1

    SciTech Connect

    Xu, Zhengyu; Wang, Tao

    2014-06-27

    Highlights: • miR-214 is upregulated in human OS tissues and inversely correlated with LZTS1 expression. • miR-214 directly targets LZTS1 by binding to its 3′-UTR. • miR-214 promotes OS cell proliferation, invasion and tumor growth. • Overexpression of LZTS1 reverses miR-214-induced proliferation and invasion of OS cells. - Abstract: Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3′-untranslated region (3′-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.

  7. MiR-132 prohibits proliferation, invasion, migration, and metastasis in breast cancer by targeting HN1

    SciTech Connect

    Zhang, Zhan-Guo Chen, Wei-Xun Wu, Yan-Hui Liang, Hui-Fang Zhang, Bi-Xiang

    2014-11-07

    Highlights: • MiR-132 is down-regulated in breast cancer tissues and cell lines. • MiR-132 directly regulates HN1 by binding its 3′ UTR. • MiR-132 shows regulatory role in proliferation, invasion, migration and metastasis. • HN1 is involved in miR-132-mediated cell behavior. • Aberrant HN1 is associated with worse overall survival of breast cancer patients. - Abstract: Accumulating evidence indicates that miRNAs play critical roles in tumorigenesis and cancer progression. This study aims to investigate the role and the underlying mechanism of miR-132 in breast cancer. Here, we report that miR-132 is significantly down-regulated in breast cancer tissues and cancer cell lines. Additional study identifies HN1 as a novel direct target of miR-132. MiR-132 down-regulates HN1 expression by binding to the 3′ UTR of HN1 transcript, thereby, suppressing multiple oncogenic traits such as cancer cell proliferation, invasion, migration and metastasis in vivo and in vitro. Overexpression of HN1 restores miR-132-suppressed malignancy. Importantly, higher HN1 expression is significantly associated with worse overall survival of breast cancer patients. Taken together, our data demonstrate a critical role of miR-132 in prohibiting cell proliferation, invasion, migration and metastasis in breast cancer through direct suppression of HN1, supporting the potential utility of miR-132 as a novel therapeutic strategy against breast cancer.

  8. miR-421 induces cell proliferation and apoptosis resistance in human nasopharyngeal carcinoma via downregulation of FOXO4

    SciTech Connect

    Chen, Liang; Department of Otolaryngology, Guangzhou General Hospital of PLA Guangzhou Command, Guangzhou 510010 ; Tang, Yanping; Wang, Jian; Yan, Zhongjie; Xu, Ruxiang

    2013-06-14

    Highlights: •miR-421 is upregulated in nasopharyngeal carcinoma. •miR-421 induces cell proliferation and apoptosis resistance. •FOXO4 is a direct and functional target of miR-421. -- Abstract: microRNAs have been demonstrated to play important roles in cancer development and progression. Hence, identifying functional microRNAs and better understanding of the underlying molecular mechanisms would provide new clues for the development of targeted cancer therapies. Herein, we reported that a microRNA, miR-421 played an oncogenic role in nasopharyngeal carcinoma. Upregulation of miR-421 induced, whereas inhibition of miR-421 repressed cell proliferation and apoptosis resistance. Furthermore, we found that upregulation of miR-421 inhibited forkhead box protein O4 (FOXO4) signaling pathway following downregulation of p21, p27, Bim and FASL expression by directly targeting FOXO4 3′UTR. Additionally, we demonstrated that FOXO4 expression is critical for miR-421-induced cell growth and apoptosis resistance. Taken together, our findings not only suggest that miR-421 promotes nasopharyngeal carcinoma cell proliferation and anti-apoptosis, but also uncover a novel regulatory mechanism for inactivation of FOXO4 in nasopharyngeal carcinoma.

  9. Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

    SciTech Connect

    Zhou, Yuan; Wang, Hui; Wang, Cong; Qiu, Xuefeng; Benson, Mikael; Yin, Xiaoqin; Xiang, Zou; Li, Dongmei; and others

    2015-08-15

    Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system. - Highlights: • miRNAs were altered in Sertoli cells exposed to MC-LR. • Alerted genes were involved in different cell functions including the cell morphology. • MC-LR adversely affected Sertoli cell junction formation through the regulating miRNAs.

  10. Characteristics of the C Shale and D Shale reservoirs, Monterey Formation, Elk Hills Field, Kern County, California

    SciTech Connect

    Reid, S.A.; McIntyre, J.L. ); McJannet, G.S. )

    1996-01-01

    The upper Miocene C Shale and D Shale reservoirs of the Elk Hills Shale Member of the Monterey Formation have cumulative oil and gas production much higher than the originally estimated recovery. These San Joaquin basin reservoirs are the lowest of the Stevens producing zones at Elk Hills and currently produce from a 2800-acre area on the 31 S anticline. The C Shale contains lower slope and basin plain deposits of very fine grained, thinly bedded, graded turbidites, pelagic and hemipelagic claystone, and slump deposits. Although all units are oil-bearing, only the lower parts of the graded turbidity intervals have sufficient horizontal permeability to produce oil. The D Shale consists of chart, claystone, carbonates and slump deposits, also originating in a lower slope to basin plain setting. All D Shale rock types contain oil, but the upper chart interval is the most productive. The chart has high matrix porosity, and due to a complex horizontal and vertical microfracture system, produces at a highly effective rate. Core samples indicate more oil-in-place is present in the thin, graded C Shale beds and in the porous D Shale chart than is identifiable from conventional electric logs. High gas recovery rates are attributed mostly to this larger volume of associated oil. Gas also enters the reservoirs from the adjacent 26R reservoir through a leaky normal fault. Significant gas volumes also may desorb from immature organic material common in the rock matrix.

  11. Characteristics of the C Shale and D Shale reservoirs, Monterey Formation, Elk Hills Field, Kern County, California

    SciTech Connect

    Reid, S.A.; McIntyre, J.L.; McJannet, G.S.

    1996-12-31

    The upper Miocene C Shale and D Shale reservoirs of the Elk Hills Shale Member of the Monterey Formation have cumulative oil and gas production much higher than the originally estimated recovery. These San Joaquin basin reservoirs are the lowest of the Stevens producing zones at Elk Hills and currently produce from a 2800-acre area on the 31 S anticline. The C Shale contains lower slope and basin plain deposits of very fine grained, thinly bedded, graded turbidites, pelagic and hemipelagic claystone, and slump deposits. Although all units are oil-bearing, only the lower parts of the graded turbidity intervals have sufficient horizontal permeability to produce oil. The D Shale consists of chart, claystone, carbonates and slump deposits, also originating in a lower slope to basin plain setting. All D Shale rock types contain oil, but the upper chart interval is the most productive. The chart has high matrix porosity, and due to a complex horizontal and vertical microfracture system, produces at a highly effective rate. Core samples indicate more oil-in-place is present in the thin, graded C Shale beds and in the porous D Shale chart than is identifiable from conventional electric logs. High gas recovery rates are attributed mostly to this larger volume of associated oil. Gas also enters the reservoirs from the adjacent 26R reservoir through a leaky normal fault. Significant gas volumes also may desorb from immature organic material common in the rock matrix.

  12. Dry Gas Zone, Elk Hills field, Kern County, California: General reservoir study: Engineering text and exhibits: (Final report)

    SciTech Connect

    Not Available

    1988-08-01

    The Dry Gas Zone in the Elk Hills field is comprised of fourteen separate productive horizons deposited in the MYA Group of the San Joaquin Formation of Pliocene Age. Eighty-six separate Reservoir Units have been identified within the interval over an area roughly ten miles long and four miles wide. One basal Tulare sand, the Tulare B, was also included in the geologic study. Five earlier studies have been made of the Dry Gas Zone; each is referenced in the Appendix of this report. Most of these studies were geologic in nature, and none provided in-depth reservoir analyses. This report is made up of ten (10) separate volumes which include: engineering text and exhibits (white dot); engineering data (black dot); geologic text and tables (green dot); structure and isochore maps (light blue dot); structural cross sections (dark blue dot); stratigraphic cross sections (brown dot); geologic data sheets -book 1 (yellow dot); geologic data sheets - book 2 (orange dot); geologic data sheets - book 3 (red dot); and geologic data sheets - book 4 (pink or coral dot). Basic production, injection, pressure, and other assorted technical data were provided by the US Department of Energy engineering staff at Elk Hills. These data were accepted as furnished with no attempt being made at independent verification.

  13. Constitutive models for the Etchegoin Sands, Belridge Diatomite, and overburden formations at the Lost Hills oil field, California

    SciTech Connect

    FOSSUM,ARLO F.; FREDRICH,JOANNE T.

    2000-04-01

    This report documents the development of constitutive material models for the overburden formations, reservoir formations, and underlying strata at the Lost Hills oil field located about 45 miles northwest of Bakersfield in Kern County, California. Triaxial rock mechanics tests were performed on specimens prepared from cores recovered from the Lost Hills field, and included measurements of axial and radial stresses and strains under different load paths. The tested intervals comprise diatomaceous sands of the Etchegoin Formation and several diatomite types of the Belridge Diatomite Member of the Monterey Formation, including cycles both above and below the diagenetic phase boundary between opal-A and opal-CT. The laboratory data are used to drive constitutive parameters for the Extended Sandler-Rubin (ESR) cap model that is implemented in Sandia's structural mechanics finite element code JAS3D. Available data in the literature are also used to derive ESR shear failure parameters for overburden formations. The material models are being used in large-scale three-dimensional geomechanical simulations of the reservoir behavior during primary and secondary recovery.

  14. Ionizing Radiation–Inducible miR-27b Suppresses Leukemia Proliferation via Targeting Cyclin A2

    SciTech Connect

    Wang, Bo; Li, Dongping; Kovalchuk, Anna; Litvinov, Dmitry; Kovalchuk, Olga

    2014-09-01

    Purpose: Ionizing radiation is a common carcinogen that is important for the development of leukemia. However, the underlying epigenetic mechanisms remain largely unknown. The goal of the study was to explore microRNAome alterations induced by ionizing radiation (IR) in murine thymus, and to determine the role of IR-inducible microRNA (miRNA/miR) in the development of leukemia. Methods and Materials: We used the well-established C57BL/6 mouse model and miRNA microarray profiling to identify miRNAs that are differentially expressed in murine thymus in response to irradiation. TIB152 human leukemia cell line was used to determine the role of estrogen receptor–α (ERα) in miR-27b transcription. The biological effects of ectopic miR-27b on leukemogenesis were measured by western immunoblotting, cell viability, apoptosis, and cell cycle analyses. Results: Here, we have shown that IR triggers the differential expression of miR-27b in murine thymus tissue in a dose-, time- and sex-dependent manner. miR-27b was significantly down-regulated in leukemia cell lines CCL119 and TIB152. Interestingly, ERα was overexpressed in those 2 cell lines, and it was inversely correlated with miR-27b expression. Therefore, we used TIB152 as a model system to determine the role of ERα in miR-27b expression and the contribution of miR-27b to leukemogenesis. β-Estradiol caused a rapid and transient reduction in miR-27b expression reversed by either ERα-neutralizing antibody or ERK1/2 inhibitor. Ectopic expression of miR-27b remarkably suppressed TIB152 cell proliferation, at least in part, by inducing S-phase arrest. In addition, it attenuated the expression of cyclin A2, although it had no effect on the levels of PCNA, PPARγ, CDK2, p21, p27, p-p53, and cleaved caspase-3. Conclusion: Our data reveal that β-estradiol/ERα signaling may contribute to the down-regulation of miR-27b in acute leukemia cell lines through the ERK1/2 pathway, and that miR-27b may function as a tumor

  15. Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression

    SciTech Connect

    Xiao, Can; Wang, Lili; Zhu, Lifang; Zhang, Chenping; Zhou, Jianhua

    2014-11-28

    Highlights: • miR-9 expression level was significantly decreased in OSCC tissues. • Curcumin significantly inhibited SCC-9 cells proliferation. • miR-9 mediates the inhibition of SCC-9 proliferation by curcumin. • Curcumin suppresses Wnt/β-catenin signaling in SCC-9 cells. • miR-9 mediates the suppression of Wnt/β-catenin signaling by curcumin. - Abstract: Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells, but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future.

  16. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  17. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    SciTech Connect

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  18. MicroRNAs expression in ox-LDL treated HUVECs: MiR-365 modulates apoptosis and Bcl-2 expression

    SciTech Connect

    Qin, Bing; Xiao, Bo; Liang, Desheng; Xia, Jian; Li, Ye; Yang, Huan

    2011-06-24

    Highlights: {yields} We evaluated the role of miRNAs in ox-LDL induced apoptosis in ECs. {yields} We found 4 up-regulated and 11 down-regulated miRNAs in apoptotic ECs. {yields} Target genes of the dysregulated miRNAs regulate ECs apoptosis and atherosclerosis. {yields} MiR-365 promotes ECs apoptosis via suppressing Bcl-2 expression. {yields} MiR-365 inhibitor alleviates ECs apoptosis induced by ox-LDL. -- Abstract: Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play a critical role in atherosclerosis. MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. However, whether miRNAs are associated with ox-LDL induced apoptosis and their effect on ECs is still unknown. Therefore, this study evaluated potential miRNAs and their involvement in ECs apoptosis in response to ox-LDL stimulation. Microarray and qRT-PCR analysis performed on human umbilical vein endothelial cells (HUVECs) exposed to ox-LDL identified 15 differentially expressed (4 up- and 11 down-regulated) miRNAs. Web-based query tools were utilized to predict the target genes of the differentially expressed miRNAs, and the potential target genes were classified into different function categories with the gene ontology (GO) term and KEGG pathway annotation. In particular, bioinformatics analysis suggested that anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl-2) is a target gene of miR-365, an apoptomir up-regulated by ox-LDL stimulation in HUVECs. We further showed that transfection of miR-365 inhibitor partly restored Bcl-2 expression at both mRNA and protein levels, leading to a reduction of ox-LDL-mediated apoptosis in HUVECs. Taken together, our findings indicate that miRNAs participate in ox-LDL-mediated apoptosis in HUVECs. MiR-365 potentiates ox-LDL-induced ECs apoptosis by regulating the

  19. IMPROVED OIL RECOVERY FROM UPPER JURASSIC SMACKOVER CARBONATES THROUGH THE APPLICATION OF ADVANCED TECHNOLOGIES AT WOMACK HILL OIL FIELD, CHOCTAW AND CLARKE COUNTIES, EASTERN GULF COASTAL PLAIN

    SciTech Connect

    Ernest A. Mancini

    2003-05-20

    Pruet Production Co. and the Center for Sedimentary Basin Studies at the University of Alabama, in cooperation with Texas A&M University, Mississippi State University, University of Mississippi, and Wayne Stafford and Associates are undertaking a focused, comprehensive, integrated and multidisciplinary study of Upper Jurassic Smackover carbonates (Class II Reservoir), involving reservoir characterization and 3-D modeling and an integrated field demonstration project at Womack Hill Oil Field Unit, Choctaw and Clarke Counties, Alabama, Eastern Gulf Coastal Plain. The principal objectives of the project are: increasing the productivity and profitability of the Womack Hill Field Unit, thereby extending the economic life of this Class II Reservoir and transferring effectively and in a timely manner the knowledge gained and technology developed from this project to producers who are operating other domestic fields with Class II Reservoirs. The principal research efforts for Year 3 of the project have been recovery technology analysis and recovery technology evaluation. The research focus has primarily been on well test analysis, 3-D reservoir simulation, microbial core experiments, and the decision to acquire new seismic data for the Womack Hill Field area. Although Geoscientific Reservoir Characterization and 3-D Geologic Modeling have been completed and Petrophysical and Engineering Characterization and Microbial Characterization are essentially on schedule, a no-cost extension until September 30, 2003, has been granted by DOE so that new seismic data for the Womack Hill Field can be acquired and interpreted to assist in the determination as to whether Phase II of the project should be implemented.

  20. EIS-0012: Petroleum Production at Maximum Efficient Rate, Naval Petroleum Reserve #1, Elk Hills, Kern County, California (also see EA-0261, EA-0334, and EIS-0158-S)

    Energy.gov [DOE]

    The U.S. Department of Energy prepared this statement to evaluate the environmental impacts of increasing petroleum production, and of additional or expanded operational facilities, at Elk Hills from 160,000 barrels per day up to 240,000 barrels per day.

  1. MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ

    SciTech Connect

    Yuan, Jian; Xiao, Gelei; Peng, Gang; Liu, Dingyang; Wang, Zeyou; Liao, Yiwei; Liu, Qing; Wu, Minghua; Yuan, Xianrui

    2015-02-06

    Highlights: • Expression of miR-125a-5p is inversely correlated with that of TAZ in glioma cells. • MiR-125a-5p represses TAZ expression in glioma cells. • MiR-125a-5p directly targets the 3′ UTR of TAZ mRNA and promotes its degradation. • MiR-125a-5p represses CTGF and survivin via TAZ, and inhibits glioma cell growth. • MiR-125a-5p inhibits the stem cell features of HFU-251 MG cells. - Abstract: Glioblastoma (GBM) is the most lethal brain tumor due to the resistance to conventional therapies, such as radiotherapy and chemotherapy. TAZ, an important mediator of the Hippo pathway, was found to be up-regulated in diverse cancers, including in GBM, and plays important roles in tumor initiation and progression. However, little is known about the regulation of TAZ expression in tumors. In this study, we found that miR-125a-5p is an important regulator of TAZ in glioma cells by directly targeting the TAZ 3′ UTR. MiR-125a-5p levels are inversely correlated with that of TAZ in normal astrocytes and a panel of glioma cell lines. MiR-125a-5p represses the expression of TAZ target genes, including CTGF and survivin, and inhibits cell proliferation and induces the differentiation of GBM cells; whereas over-expression of TAZ rescues the effects of miR-125a-5p. This study revealed a mechanism for TAZ deregulation in glioma cells, and also demonstrated a tumor suppressor role of miR-125a-5p in glioblastoma cells.

  2. The NuMI proton beam at Fermilab successes and challenges

    SciTech Connect

    Childress, S.; /Fermilab

    2008-11-01

    The NuMI beam at Fermilab has delivered over 5 x 10{sup 20} 120 GeV protons to the neutrino production target since the start for MINOS [1] neutrino oscillation experiment operation in 2005. We report on proton beam commissioning and operation status, including successes and challenges with this beam.

  3. DOE Zero Energy Ready Home Case Study: Cobblestone Homes, Midland, MI

    Office of Energy Efficiency and Renewable Energy (EERE)

    Case study of a DOE Zero Energy Ready home in Midland, MI, that scored HERS 49 without PV or HERS 44 with 1.4 kW of PV. The custom home served as a prototype and energy efficiency demonstration...

  4. MiR-18a regulates the proliferation, migration and invasion of human glioblastoma cell by targeting neogenin

    SciTech Connect

    Song, Yichen; Wang, Ping; Zhao, Wei; Yao, Yilong; Liu, Xiaobai; Ma, Jun; Xue, Yixue; Liu, Yunhui

    2014-05-15

    MiR-17-92 cluster has recently been reported as an oncogene in some tumors. However, the association of miR-18a, an important member of this cluster, with glioblastoma remains unknown. Therefore, this study aims to investigate the expression of miR-18a in glioblastoma and its role in biological behavior of U87 and U251 human glioblastoma cell lines. Quantitative RT-PCR results showed that miR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines compared with that in human brain tissues and primary normal human astrocytes, and the expression levels were increased along with the rising pathological grades of glioblastoma. Neogenin was identified as the target gene of miR-18a by dual-luciferase reporter assays. RT-PCR and western blot results showed that its expression levels were decreased along with the rising pathological grades of glioblastoma. Inhibition of miR-18a expression was established by transfecting exogenous miR-18a inhibitor into U87 and U251 cells, and its effects on the biological behavior of glioblastoma cells were studied using CCK-8 assay, transwell assay and flow cytometry. Inhibition of miR-18a expression in U87 and U251 cells significantly up-regulated neogenin, and dramatically suppressed the abilities of cell proliferation, migration and invasion, induced cell cycle arrest and promoted cellular apoptosis. Collectively, these results suggest that miR-18a may regulate biological behavior of human glioblastoma cells by targeting neogenin, and miR-18a can serve as a potential target in the treatment of glioblastoma. - Highlights: • MiR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines. • Neogenin was identified as the target gene of miR-18a. • Neogenin expressions were decreased along with the rising pathological grades of glioblastoma. • Inhibition of miR-18a suppressed biological behavior of glioma cells by up-regulating neogenin.

  5. miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer

    SciTech Connect

    Li, Qiaoyan; Zhu, Fufan; Chen, Puxiang

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Both miR-7 and miR-218 down-regulates HoxB3 expression by targeting the 3 Prime -UTR of HoxB3 mRNA. Black-Right-Pointing-Pointer A reverse correlation between the levels of endogenous miR-7, miR218 and HoxB3 expression. Black-Right-Pointing-Pointer Epigenetic changes involve in the reactivation of HoxB3. Black-Right-Pointing-Pointer Both miRNAs inhibits the cell cycle and clone formation of breast cancer cells. -- Abstract: Many microRNAs have been implicated as key regulators of cellular growth and differentiation and have been found to dysregulate proliferation in human tumors, including breast cancer. Cancer-linked microRNAs also alter the epigenetic landscape by way of DNA methylation and post-translational modifications of histones. Aberrations in Hox gene expression are important for oncogene or tumor suppressor during abnormal development and malignancy. Although recent studies suggest that HoxB3 is critical in breast cancer, the putative role(s) of microRNAs impinging on HoxB3 is not yet fully understood. In this study, we found that the expression levels of miR-7 and miR-218 were strongly and reversely associated with HoxB3 expression. Stable overexpression of miR-7 and miR-218 was accompanied by reactivation of tumor suppressor genes including RASSF1A and Claudin-6 by means of epigenetic switches in DNA methylation and histone modification, giving rise to inhibition of the cell cycle and clone formation of breast cancer cells. The current study provides a novel link between overexpression of collinear Hox genes and multiple microRNAs in human breast malignancy.

  6. Loss of expression of miR-335 is implicated in hepatic stellate cell migration and activation

    SciTech Connect

    Chen, Chao; Wu, Chao-Qun; Zhang, Zong-Qi; Yao, Ding-Kang; Zhu, Liang

    2011-07-15

    Activation and migration of resident stellate cells (HSCs) within the hepatic space of Disse play an important role in hepatic fibrosis, which accounts for the increased numbers of activated HSCs in areas of inflammation during hepatic fibrosis. Currently, microRNAs have been found to play essential roles in HSC differentiation, proliferation, apoptosis, fat accumulation and collagen production. However, little is known about microRNA mediated HSC activation and migration. In this study, the miRNA expression profiles of quiescent HSCs, partially activated HSCs and fully activated HSCs were compared in pairs. Gene ontology (GO) and GO-Map network analysis indicated that the activation of HSCs was regulated by microRNAs. Among them miR-335 was confirmed to be significantly reduced during HSC activation by qRT-PCR, and restoring expression of miR-335 inhibited HSC migration and reduced {alpha}-SMA and collagen type I. Previous study revealed that tenascin-C (TNC), an extracellular matrix glycoprotein involved in cell migration, might be a target of miR-335. Therefore, we further studied the TNC expression in miR-335 over-expressed HSCs. Our data showed that exogenous TNC could enhance HSC migration in vitro and miR-335 restoration resulted in a significant inhibition of TNC expression. These results demonstrated that miR-335 restoration inhibited HSC migration, at least in part, via downregulating the TNC expression.

  7. PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

    SciTech Connect

    Zheng, Peihao; Guo, Honggang; Li, Guangchao; Han, Siqi; Luo, Fei; Liu, Yi

    2015-03-06

    Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.

  8. RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR...

    Office of Scientific and Technical Information (OSTI)

    in prostate cancer Citation Details In-Document Search Title: RLIP76-dependent suppression of PI3KAKTBcl-2 pathway by miR-101 induces apoptosis in prostate cancer MicroRNA-101 ...

  9. Aryl hydrocarbon receptor-dependent regulation of miR-196a expression controls lung fibroblast apoptosis but not proliferation

    SciTech Connect

    Hecht, Emelia; Zago, Michela; Sarill, Miles; Rico de Souza, Angela; Gomez, Alvin; Matthews, Jason; Hamid, Qutayba; Eidelman, David H.; Baglole, Carolyn J.

    2014-11-01

    The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor implicated in the regulation of apoptosis and proliferation. Although activation of the AhR by xenobiotics such as dioxin inhibits the cell cycle and control apoptosis, paradoxically, AhR expression also promotes cell proliferation and survival independent of exogenous ligands. The microRNA (miRNA) miR-196a has also emerged as a regulator of proliferation and apoptosis but a relationship between the AhR and miR-196a is not known. Therefore, we hypothesized that AhR-dependent regulation of endogenous miR-196a expression would promote cell survival and proliferation. Utilizing lung fibroblasts from AhR deficient (AhR{sup −/−}) and wild-type (AhR{sup +/+}) mice, we show that there is ligand-independent regulation of miRNA, including low miR-196a in AhR{sup −/−} cells. Validation by qRT-PCR revealed a significant decrease in basal expression of miR-196a in AhR{sup −/−} compared to AhR{sup +/+} cells. Exposure to AhR agonists benzo[a]pyrene (B[a]P) and FICZ as well as AhR antagonist CH-223191 decreased miR-196a expression in AhR{sup +/+} fibroblasts concomitant with decreased AhR protein levels. There was increased proliferation only in AhR{sup +/+} lung fibroblasts in response to serum, corresponding to a decrease in p27{sup KIP1} protein, a cyclin-dependent kinase inhibitor. Increasing the cellular levels of miR-196a had no effect on proliferation or expression of p27{sup KIP1} in AhR{sup −/−} fibroblasts but attenuated cigarette smoke-induced apoptosis. This study provides the first evidence that AhR expression is essential for the physiological regulation of cellular miRNA levels- including miR-196a. Future experiments designed to elucidate the functional relationship between the AhR and miR-196a may delineate additional novel ligand-independent roles for the AhR. - Highlights: • The AhR controls proliferation and apoptosis in lung cells. • The AhR regulates the

  10. Material Activation Benchmark Experiments at the NuMI Hadron Absorber Hall in Fermilab

    SciTech Connect

    Matsumura, H.; Matsuda, N.; Kasugai, Y.; Toyoda, A.; Yashima, H.; Sekimoto, S.; Iwase, H.; Oishi, K.; Sakamoto, Y.; Nakashima, H.; Leveling, A.; Boehnlein, D.; Lauten, G.; Mokhov, N.; Vaziri, K.

    2014-06-15

    In our previous study, double and mirror symmetric activation peaks found for Al and Au arranged spatially on the back of the Hadron absorber of the NuMI beamline in Fermilab were considerably higher than those expected purely from muon-induced reactions. From material activation bench-mark experiments, we conclude that this activation is due to hadrons with energy greater than 3 GeV that had passed downstream through small gaps in the hadron absorber.

  11. IMPROVED OIL RECOVERY FROM UPPER JURASSIC SMACKOVER CARBONATES THROUGH THE APPLICATION OF ADVANCED TECHNOLOGIES AT WOMACK HILL OIL FIELD, CHOCTAW AND CLARKE COUNTIES, ALABAMA, EASTERN GULF COASTAL PLAIN

    SciTech Connect

    Ernest A. Mancini

    2004-12-06

    The principal research effort for Year 1 of the project is drilling an infill well in the Womack Hill Field, Choctaw and Clarke Counties, Alabama. The objectives of the project are to drill and core an infill well in Womack Hill Field; to utilize samples from the core to evaluate further the feasibility of implementing an immobilized enzyme technology project in the field; and to use the new information resulting from the drilling of the well to revise and modify the 3-D geologic model, to further modify the injection strategy for the existing pressure maintenance program, and to assess whether a second infill well should be drilled using lateral/multilateral well completions.

  12. IMPROVED OIL RECOVERY FROM UPPER JURASSIC SMACKOVER CARBONATES THROUGH THE APPLICATION OF ADVANCED TECHNOLOGIES AT WOMACK HILL OIL FIELD, CHOCTAW AND CLARKE COUNTIES, ALABAMA, EASTERN GULF COASTAL PLAIN

    SciTech Connect

    Ernest A. Mancini

    2004-12-13

    The principal research effort for Year 1 of the project is drilling an infill well in the Womack Hill Field, Choctaw and Clarke Counties, Alabama. The objectives of the project are to drill and core an infill well in Womack Hill Field; to utilize samples from the core to evaluate further the feasibility of implementing an immobilized enzyme technology project in the field; and to use the new information resulting from the drilling of the well to revise and modify the 3-D geologic model, to further modify the injection strategy for the existing pressure maintenance program, and to assess whether a second infill well should be drilled using lateral/multilateral well completions.

  13. Measurement of Pi-K Ratios from the NuMI Target

    SciTech Connect

    Seun, Sin Man

    2007-07-01

    Interactions of protons (p) with the NuMI (Neutrinos at the Main Injector) target are used to create the neutrino beam for the MINOS (Main Injector Neutrino Oscillation Search) Experiment. Using the MIPP (Main Injector Particle Production) experimental apparatus, the production of charged pions and kaons in p+NuMI interactions is studied. The data come from a sample of 2 x 106 events obtained by MIPP using the 120 GeV/c proton beam from the Main Injector at Fermi National Accelerator Laboratory in Illinois, USA. Pions and kaons are identified by measurement in a Ring Imaging Cherenkov detector. Presented are measurements of π-+, K-/K+, π+/K+ and π-/K- production ratios in the momentum range pT < 2 GeV/c transversely and 20 GeV/c < pz < 90 GeV/c longitudinally. Also provided are detailed comparisons of the MIPP NuMI data with the MIPP Thin Carbon data, the MIPP Monte Carlo simulation and the current MINOS models in the relevant momentum ranges.

  14. miR-21 modulates tumor outgrowth induced by human adipose tissue-derived mesenchymal stem cells in vivo

    SciTech Connect

    Shin, Keun Koo; Lee, Ae Lim; Kim, Jee Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870; BK21 Medical Science Education Center, School of Medicine, Pusan National University, Yangsan, Gyeongnam 626-870 ; Lee, Sun Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870 ; Bae, Yong Chan; Jung, Jin Sup

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer miR-21 modulates hADSC-induced increase of tumor growth. Black-Right-Pointing-Pointer The action is mostly mediated by the modulation of TGF-{beta} signaling. Black-Right-Pointing-Pointer Inhibition of miR-21 enhances the blood flow recovery in hindlimb ischemia. -- Abstract: Mesenchymal stem cells (MSCs) have generated a great deal of interest in clinical situations, due principally to their potential use in regenerative medicine and tissue engineering applications. However, the therapeutic application of MSCs remains limited, unless the favorable effects of MSCs on tumor growth in vivo, and the long-term safety of the clinical applications of MSCs, can be more thoroughly understood. In this study, we determined whether microRNAs can modulate MSC-induced tumor outgrowth in BALB/c nude mice. Overexpression of miR-21 in human adipose-derived stem cells (hADSCs) inhibited hADSC-induced tumor growth, and inhibition of miR-21 increased it. Downregulation of transforming growth factor beta receptor II (TGFBR2), but not of signal transducer and activator of transcription 3, in hADSCs showed effects similar to those of miR-21 overexpression. Downregulation of TGFBR2 and overexpression of miR21 decreased tumor vascularity. Inhibition of miR-21 and the addition of TGF-{beta} increased the levels of vascular endothelial growth factor and interleukin-6 in hADSCs. Transplantation of miR-21 inhibitor-transfected hADSCs increased blood flow recovery in a hind limb ischemia model of nude mice, compared with transplantation of control oligo-transfected cells. These findings indicate that MSCs might favor tumor growth in vivo. Thus, it is necessary to study the long-term safety of this technique before MSCs can be used as therapeutic tools in regenerative medicine and tissue engineering.

  15. Targeting miR-21 enhances the sensitivity of human colon cancer HT-29 cells to chemoradiotherapy in vitro

    SciTech Connect

    Deng, Jun; Lei, Wan; Fu, Jian-Chun; Zhang, Ling; Li, Jun-He; Xiong, Jian-Ping

    2014-01-17

    Highlight: MiR-21 plays a significant role in 5-FU resistance. This role might be attributed to targeting of hMSH2 as well as TP and DPD via miR-21 targeted hMSH2. Indirectly targeted TP and DPD to influence 5-FU chemotherapy sensitivity. -- Abstract: 5-Fluorouracil (5-FU) is a classic chemotherapeutic drug that has been widely used for colorectal cancer treatment, but colorectal cancer cells are often resistant to primary or acquired 5-FU therapy. Several studies have shown that miR-21 is significantly elevated in colorectal cancer. This suggests that this miRNA might play a role in this resistance. In this study, we investigated this possibility and the possible mechanism underlying this role. We showed that forced expression of miR-21 significantly inhibited apoptosis, enhanced cell proliferation, invasion, and colony formation ability, promoted G1/S cell cycle transition and increased the resistance of tumor cells to 5-FU and X radiation in HT-29 colon cancer cells. Furthermore, knockdown of miR-21 reversed these effects on HT-29 cells and increased the sensitivity of HT-29/5-FU to 5-FU chemotherapy. Finally, we showed that miR-21 targeted the human mutS homolog2 (hMSH2), and indirectly regulated the expression of thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD). These results demonstrate that miR-21 may play an important role in the 5-FU resistance of colon cancer cells.

  16. Dry Gas Zone, Elk Hills Field, Kern County, California: General reservoir study: Geologic text and tables: Final report

    SciTech Connect

    Not Available

    1988-06-29

    The Dry Gas Zone was defined by US Naval Petroleum Reserve No. 1 Engineering Committee (1957) as ''/hor ellipsis/all sands bearing dry gas above the top of the Lower Scalez marker bed. The term is used to include the stratigraphic interval between the Scalez Sand Zone and the Tulare Formation - the Mya Sand Zone. The reservoirs in this upper zone are thin, lenticular, loosely cemented sandstones with relatively high permeabilities.'' Other than the limited Tulare production in the western part of the field, the Dry Gas Zone is the shallowest productive zone in the Elk Hills Reserve and is not included in the Shallow Oil Zone. It is Pliocene in age and makes up approximately eighty percent of the San Joaquin Formation as is summarized in Exhibit TL-1. The lithologic character of the zone is one of interbedded shales and siltstones with intermittent beds of various thickness sands. The stratigraphic thickness of the Dry Gas Zone ranges from 950 to 1150 feet with a general thickening along the flanks and thinning over the crests of the anticlines. The productive part of the Dry Gas Zone covers portions of 30 sections in an area roughly 10 miles long by 4 miles wide. 4 refs.

  17. Sonar atlas of caverns comprising the U.S. Strategic Petroleum Reserve. Volume 2, Big Hill Site, Texas.

    SciTech Connect

    Rautman, Christopher Arthur; Lord, Anna Snider

    2007-08-01

    Downhole sonar surveys from the four active U.S. Strategic Petroleum Reserve sites have been modeled and used to generate a four-volume sonar atlas, showing the three-dimensional geometry of each cavern. This volume 2 focuses on the Big Hill SPR site, located in southeastern Texas. Volumes 1, 3, and 4, respectively, present images for the Bayou Choctaw SPR site, Louisiana, the Bryan Mound SPR site, Texas, and the West Hackberry SPR site, Louisiana. The atlas uses a consistent presentation format throughout. The basic geometric measurements provided by the down-cavern surveys have also been used to generate a number of geometric attributes, the values of which have been mapped onto the geometric form of each cavern using a color-shading scheme. The intent of the various geometrical attributes is to highlight deviations of the cavern shape from the idealized cylindrical form of a carefully leached underground storage cavern in salt. The atlas format does not allow interpretation of such geometric deviations and anomalies. However, significant geometric anomalies, not directly related to the leaching history of the cavern, may provide insight into the internal structure of the relevant salt dome.

  18. miR-502 inhibits cell proliferation and tumor growth in hepatocellular carcinoma through suppressing phosphoinositide 3-kinase catalytic subunit gamma

    SciTech Connect

    Chen, Suling; Li, Fang; Chai, Haiyun; Tao, Xin; Wang, Haili; Ji, Aifang

    2015-08-21

    MicroRNAs (miRNAs) play a key role in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). In the present study, we demonstrated that miR-502 significantly inhibits HCC cell proliferation in vitro and tumor growth in vivo. G1/S cell cycle arrest and apoptosis of HCC cells were induced by miR-502. Phosphoinositide 3-kinase catalytic subunit gamma (PIK3CG) was identified as a direct downstream target of miR-502 in HCC cells. Notably, overexpression of PIK3CG reversed the inhibitory effects of miR-502 in HCC cells. Our findings suggest that miR-502 functions as a tumor suppressor in HCC via inhibition of PI3KCG, supporting its utility as a promising therapeutic gene target for this tumor type. - Highlights: • miR-502 suppresses HCC cell proliferation in vitro and tumorigenicity in vivo. • miR-502 regulates cell cycle and apoptosis in HCC cells. • PIK3CG is a direct target of miR-502. • miR-502 and PIK3CG expression patterns are inversely correlated in HCC tissues.

  19. RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR-101 induces apoptosis in prostate cancer

    SciTech Connect

    Yang, Jing; Song, Qi; Cai, Yi; Wang, Peng; Wang, Min; Zhang, Dong

    2015-08-07

    MicroRNA-101 (miR-101) participates in carcinogenesis and tumor progression in various cancers. However, its biological functions in prostate cancer are still unclear. Here, we demonstrate that miR-101 represents a critical role in regulating cell apoptosis in prostate cancer cells. We first demonstrated that miR-101 treatment promoted apoptosis in DU145 and PC3 cells by using flow cytometric analysis and transmission electron microscopy (TEM). To verify the mechanisms, we identified a novel miR-101 target, Ral binding protein 1 (RLIP76). We found miR-101 transfection significantly suppresses RLIP76 expression, which can transactivate phosphorylation of PI3K-Akt signaling, and resulted in an amplification of Bcl2-induced apoptosis. Furthermore, we demonstrated that RLIP76 overexpression could reverse the anti-tumor effects of miR-101 in DU145 and PC3 cells by using flow cytometry assay and MTT assay. Taken together, our results revealed that the effect of miR-101 on prostate cancer cell apoptosis was due to RLIP76 regulation of the PI3K/Akt/Bcl-2 signaling pathway. - Highlights: • miR-101 inhibited prostate cancer cell proliferation and enhanced apoptosis. • miR-101 directly targeted and regulated RLIP76 expression. • miR-101 suppressed PI3K/Akt/Bcl-2 signaling pathway by targeting RLIP76.

  20. MiR-20a Induces Cell Radioresistance by Activating the PTEN/PI3K/Akt Signaling Pathway in Hepatocellular Carcinoma

    SciTech Connect

    Zhang, Yuqin; Zheng, Lin; Ding, Yi; Li, Qi; Wang, Rong; Liu, Tongxin; Sun, Quanquan; Yang, Hua; Peng, Shunli; Wang, Wei; Chen, Longhua

    2015-08-01

    Purpose: To investigate the role of miR-20a in hepatocellular carcinoma (HCC) cell radioresistance, which may reveal potential strategies to improve treatment. Methods and Materials: The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction. Cell radiation combined with colony formation assays was administrated to discover the effect of miR-20a on radiosensitivity. Bioinformatics prediction and luciferase assay were used to identify the target of miR-20a. The phosphatidylinositol 3-kinase inhibitor LY294002 was used to inhibit phosphorylation of Akt, to verify whether miR-20a affects HCC cell radioresistance through activating the PTEN/PI3K/Akt pathway. Results: MiR-20a levels were increased in HCC cell lines and tissues, whereas PTEN was inversely correlated with it. Overexpression of miR-20a in Bel-7402 and SMMC-7721 cells enhances their resistance to the effect of ionizing radiation, and the inhibition of miR-20a in HCCLM3 and QGY-7701 cells sensitizes them to it. PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance. Overexpression of miR-20a activated the PTEN/PI3K/Akt signaling pathway. Additionally, the kinase inhibitor LY294002 could reverse the effect of miR-20a–induced radioresistance. Conclusion: MiR-20a induces HCC cell radioresistance by activating the PTEN/PI3K/Akt pathway, which suggests that miR-20a/PTEN/PI3K/Akt might represent a target of investigation for developing effective therapeutic strategies against HCC.

  1. MiR-145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1

    SciTech Connect

    Wu, Huijuan; Xiao, ZhengHua; Wang, Ke; Liu, Wenxin; Hao, Quan

    2013-11-29

    Highlights: •MiR-145 is downregulated in human ovarian cancer. •MiR-145 targets p70S6K1 and MUC1. •p70S6K1 and MUC1 are involved in miR-145 mediated tumor cell growth and cell invasion, respectively. -- Abstract: MicroRNAs (miRNAs) are a family of small non-coding RNA molecules that regulate gene expression at post-transcriptional levels. Previous studies have shown that miR-145 is downregulated in human ovarian cancer; however, the roles of miR-145 in ovarian cancer growth and invasion have not been fully demonstrated. In the present study, Northern blot and qRT-PCR analysis indicate that miR-145 is downregulated in ovarian cancer tissues and cell lines, as well as in serum samples of ovarian cancer, compared to healthy ovarian tissues, cell lines and serum samples. Functional studies suggest that miR-145 overexpression leads to the inhibition of colony formation, cell proliferation, cell growth viability and invasion, and the induction of cell apoptosis. In accordance with the effect of miR-145 on cell growth, miR-145 suppresses tumor growth in vivo. MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3′UTRs. Importantly, the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145, respectively. MiR-145 expression is increased after 5-aza-CdR treatment, and 5-aza-CdR treatment results in the same phenotype as the effect of miR-145 overexpression. Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1, functioning as a tumor suppressor. Moreover, our data imply that miR-145 has potential as a miRNA-based therapeutic target for ovarian cancer.

  2. Petrography, mineralogy, and chemistry of calcite-silica deposits at Exile Hill, Nevada, compared with local spring deposits

    SciTech Connect

    Vaniman, D.T.; Chipera, S.J.; Bish, D.L.

    1995-12-01

    Chemical, mineralogic, and petrographic analyses of siliceous calcretes from Exile Hill east of Yucca Mountain, Nevada, indicate that pedogenic processes alone account for the formation of the calcretes. These calcretes have been interpreted by some observers as evidence of seismically triggered eruptions of deep water. Such an origin could have important consequences if Yucca Mountain is developed as an unsaturated site for the disposal of high-level nuclear waste. At odds with this hypothesis are the absence of features that should be present at fault-fed springs (e.g., fissure-ridge mounds with microterraces) and the preservation within root casts of delicate pedogenic microfossils, such as calcified filaments and needle-fiber calcites. Mineral-chemical evidence of pedogenic origin is found in heavy-mineral concentrations, reflected in Fe and Sc enrichments. These concentrations, which occur in the most massive of the vein calcretes, require derivation of detritus from a mixture of weathered and eolian materials that occur in the overlying B soil horizons, as opposed to direct incorporation of adjacent unweathered bedrock. Carbonate and silica abundances and accumulation rates are well within the scope of pedogenic processes. Calcium is derived from rainwater or eolian sources, whereas silica is derived in part by dissolution of local volcanic glasses or from dissolution of unstable silica minerals that are abundant in the local tuffs. In contrast with local deposits that are of spring or seep origin, the siliceous calcretes at Yucca Mountain are pedogenic in origin as well as evolution and provide no evidence in support of conjectured spring activity.

  3. Application of horizontal drilling in the development of a complex turbidite sandstone reservoir, Elk Hills Field, Kern County, California

    SciTech Connect

    Reid, S.A. ); McJannet, G.S. ); Hart, O.D. )

    1990-05-01

    Horizontal drilling techniques have been used at the Elk Hills field, to more effectively produce the complex 26R reservoir. This Stevens zone reservoir of the Miocene Monterey Formation contains turbid sediments deposited in a deep-sea fan setting and consists of several distinct sandstone layers averaging 150 ft thick and usually separated by mudstone beds. Layers in the reservoir dip as much as 50{degree} southwest. An expanding gas cap makes many vertical wells less favorable to operate. Horizontal completions were thought ideal for the pool because (1) original oil-water contact is level and believed stable, (2) water production is low, (3) a horizontal well provides for a long production life; and (4) several sandstone layers can be produced through one well. For the first well, the plan was to redrill an idle well to horizontal along an arc with a radius of 350 ft. The horizontal section was to be up to 1,000 ft long and extend northeast slightly oblique to dip just above the average oil-water contact. The well was drilled in September 1988, reached horizontal nearly as planned, was completed after perforating 210 ft of oil sand, and produced a daily average of 1,000 bbl oil and 8 bbl of water. However, structural influence was stronger than expected, causing the horizontal drill path to turn directly updip away from the bottom-hole target area. The well also encountered variable oil-water contacts, with more than half the horizontal section possibly water productive. Geologic and drilling data from the first well were used for planning another well. This well was drilled in October 1989, and was highly successful with over 1,000 ft of productive interval.

  4. Mass transfer during wall-rock alteration: An example from a quartz-graphite vein, Black Hills, South Dakota

    SciTech Connect

    Galbreath, K.C.; Duke, E.F.; Papike, J.J. ); Laul, J.C. )

    1988-07-01

    Mass transfer and fluid-rock interaction have been evaluated along two sample traverses in low-sillimanite grade quartz-mica schist adjacent to a synmetamorphic quartz-graphite vein in the southern Black Hills, South Dakota. In an {approximately}17 cm halo between apparently unaltered schist and the vein contact is an outer zone of cryptic alteration and three inner zones of visible alteration. The cryptic zone consists of the original prograde metamorphic mineral assemblage plus anomalously high amounts of tourmaline. The outermost visible zone contains abundant graphite. The second visible zone is defined by intensive bleaching of the schist. The innermost visible zone, immediately adjacent to the vein, is tourmaline + quartz + plagioclase + limonite + graphite. The vein is composed almost entirely of quartz, but also contains trace amounts of graphite. Mass balance calculations indicate that Al was essentially inert. The predominant chemical changes during wall-rock alteration were addition of B and C from the vein-forming fluid along with loss of K from the wall rocks, corresponding to precipitation of tourmaline and graphite, and the progressive destruction of microcline, biotite, and muscovite toward the vein. In addition, the elements V, Cr, Cu, Zn, Pb, As, Sb, W, and Au were introduced into the country rock, whereas Si, Rb, Ba, and Cs were removed. Fluid-rock interaction modeling suggests that between one and four equivalent masses of fluid interacted chemically with the most altered mineral assemblages. In addition, greater than one equivalent mass of reactive fluid penetrated to distances of at least 5 cm from the vein contact.

  5. Hydrothermal energy extraction, Auburn, New York: Final report: Volume 2, Chapters 6-10

    SciTech Connect

    Castor, T.P.

    1988-03-01

    This paper discusses a hydrothermal energy extraction system in detail. General topics covered are: Reservoir circulation loop; HVAC buffer loop; and automatic temperature control system. (LSP)

  6. The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1

    SciTech Connect

    Zhuang, Ming; Gao, Wen; Xu, Jing; Wang, Ping; Shu, Yongqian

    2014-06-06

    Graphical abstract: - Highlights: • H19 regulates gastric cancer cell proliferation phenotype via miR-675. • MiR-675 modulates cell proliferation of gastric cancer cells by targeting tumor suppressor RUNX1. • The H19/miR-675/RUNX1 axis plays an important role in the tumorigenesis of gastric cancer. - Abstract: The lncRNA H19 has been recently shown to be upregulated and play important roles in gastric cancer tumorigenesis. However, the precise molecular mechanism of H19 and its mature product miR-675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that miR-675 was positively expressed with H19 and was a pivotal mediator in H19-induced gastric cancer cell growth promotion. Subsequently, the tumor suppressor Runt Domain Transcription Factor1 (RUNX1) was confirmed to be a direct target of miR-675 using a luciferase reporter assay and Western blotting analyses. A series of rescue assays indicated that RUNX1 mediated H19/miR-67-induced gastric cancer cell phenotypic changes. Moreover, the inverse relationship between the expression of RUNX1 and H19/miR-675 was also revealed in gastric cancer tissues and gastric cancer cell lines. Taken together, our study demonstrated that the novel pathway H19/miR-675/RUNX1 regulates gastric cancer development and may serve as a potential target for gastric cancer therapy.

  7. Testing CPT conservation using the NuMI neutrino beam with the MINOS experiment

    SciTech Connect

    Auty, David John

    2010-05-01

    The MINOS experiment was designed to measure neutrino oscillation parameters with muon neutrinos. It achieves this by measuring the neutrino energy spectrum and flavor composition of the man-made NuMI neutrino beam 1km after the beam is formed and again after 735 km. By comparing the two spectra it is possible to measure the oscillation parameters. The NuMI beam is made up of 7.0% {bar {nu}}{sub {mu}}, which can be separated from the {nu}{sub {mu}} because the MINOS detectors are magnetized. This makes it possible to study {bar {nu}}{sub {mu}} oscillations separately from those of muon neutrinos, and thereby test CPT invariance in the neutrino sector by determining the {bar {nu}}{sub {mu}} oscillation parameters and comparing them with those for {nu}{sub {mu}}, although any unknown physics of the antineutrino would appear as a difference in oscillation parameters. Such a test has not been performed with beam {bar {nu}}{sub {mu}} before. It is also possible to produce an almost pure {bar {nu}}{sub {mu}} beam by reversing the current through the magnetic focusing horns of the NuMI beamline, thereby focusing negatively, instead of positively charged particles. This thesis describes the analysis of the 7% {bar {nu}}{sub {mu}} component of the forward horn current NuMI beam. The {bar {nu}}{sub {mu}} of a data sample of 3.2 x 10{sup 20} protons on target analysis found 42 events, compared to a CPT conserving prediction of 58.3{sub -7.6}{sup +7.6}(stat.){sub -3.6}{sup +3.6}(syst.) events. This corresponds to a 1.9 {sigma} deficit, and a best fit value of {Delta}{bar m}{sub 32}{sup 2} = 18 x 10{sup -3} eV{sup 2} and sin{sup 2} 2{bar {theta}}{sub 23} = 0.55. This thesis focuses particularly on the selection of {bar {nu}}{sub {mu}} events, and investigates possible improvements of the selection algorithm. From this a different selector was chosen, which corroborated the findings of the original selector. The thesis also investigates how the systematic errors affect the

  8. ,"Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf)"

    Energy Information Administration (EIA) (indexed site)

    Imports From Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  9. ,"Marysville, MI Natural Gas Pipeline Imports From Canada (MMcf)"

    Energy Information Administration (EIA) (indexed site)

    Imports From Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","Marysville, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  10. Port Huron, MI Natural Gas Pipeline Imports From Canada (Dollars per

    Energy Information Administration (EIA) (indexed site)

    Thousand Cubic Feet) Dollars per Thousand Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Dollars per Thousand Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 2.07 2.06 2.21 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Price of

  11. ,"St. Clair, MI Natural Gas Pipeline Exports to Canada (MMcf)"

    Energy Information Administration (EIA) (indexed site)

    Exports to Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","St. Clair, MI Natural Gas Pipeline Exports to Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  12. ,"St. Clair, MI Natural Gas Pipeline Imports From Canada (MMcf)"

    Energy Information Administration (EIA) (indexed site)

    Imports From Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","St. Clair, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  13. Assessment of radiological releases from the NuMI facility during MINOS and NOvA operations

    SciTech Connect

    Martens, Mike; /Fermilab

    2007-04-01

    This report makes projections of the radiological releases from the NuMI facility during operations for the MINOS and NO ?A experiments. It includes an estimate of the radionuclide levels released into the atmosphere and the estimated tritium and sodium-22 concentrations in the NuMI sump water and Fermilab pond system. The analysis was performed for NuMI operations with a beam power on target increased from the present 400 kW design up to a possible 1500 kW with future upgrades. The total number of protons on target was assumed to be 18 x 10{sup 20} after the completion of MINOS and 78 x 10{sup 20} after the completion of NO ?A.

  14. Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene

    SciTech Connect

    Zhang, Wei Wei; Tong, Hui Li; Sun, Xiao Feng; Hu, Qian; Yang, Yu; Li, Shu Feng; Yan, Yun Qin; Li, Guang Peng

    2015-08-07

    MicroRNAs play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. Previous study in our laboratory showed that the expression level of miR-2400, a novel and unique miRNA from bovine, had significantly changed in skeletal muscle-derived satellite cells (MDSCs) during differentiation, however, the function and expression pattern for miR-2400 in MDSCs has not been fully understood. In this report, we firstly identified that the expression levels of miR-2400 were down-regulated during MDSCs differentiation by stem-loop RT-PCR. Over-expression and inhibition studies demonstrated that miR-2400 promoted MDSCs proliferation by EdU (5-ethynyl-2′ deoxyuridine) incorporation assay and immunofluorescence staining of Proliferating cell nuclear antigen (PCNA). Luciferase reporter assays showed that miR-2400 directly targeted the 3′ untranslated regions (UTRs) of myogenin (MYOG) mRNA. These data suggested that miR-2400 could promote MDSCs proliferation through targeting MYOG. Furthermore, we found that miR-2400, which was located within the eighth intron of the Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1) gene, was down-regulated in MDSCs in a direct correlation with the WHSC1L1 transcript by Clustered regularly interspaced palindromic repeats interference (CRISPRi). In addition, these observations not only provided supporting evidence for the codependent expression of intronic miRNAs and their host genes in vitro, but also gave insight into the role of miR-2400 in MDSCs proliferation. - Highlights: • miR-2400 is a novel and unique miRNA from bovine. • miR-2400 could promote skeletal muscle satellite cells proliferation. • miR-2400 directly targeted the 3′ untranslated regions of MYOG mRNA. • miR-2400 could be coexpressed together with its host gene WHSC1L1.

  15. miR-340 inhibits glioblastoma cell proliferation by suppressing CDK6, cyclin-D1 and cyclin-D2

    SciTech Connect

    Li, Xuesong; Gong, Xuhai; Chen, Jing; Zhang, Jinghui; Sun, Jiahang; Guo, Mian

    2015-05-08

    Glioblastoma development is often associated with alteration in the activity and expression of cell cycle regulators, such as cyclin-dependent kinases (CKDs) and cyclins, resulting in aberrant cell proliferation. Recent studies have highlighted the pivotal roles of miRNAs in controlling the development and growth of glioblastoma. Here, we provide evidence for a function of miR-340 in the inhibition of glioblastoma cell proliferation. We found that miR-340 is downregulated in human glioblastoma tissue samples and several established glioblastoma cell lines. Proliferation and neurosphere formation assays revealed that miR-340 plays an oncosuppressive role in glioblastoma, and that its ectopic expression causes significant defect in glioblastoma cell growth. Further, using bioinformatics, luciferase assay and western blot, we found that miR-340 specifically targets the 3′UTRs of CDK6, cyclin-D1 and cyclin-D2, leading to the arrest of glioblastoma cells in the G0/G1 cell cycle phase. Confirming these results, we found that re-introducing CDK6, cyclin-D1 or cyclin-D2 expression partially, but significantly, rescues cells from the suppression of cell proliferation and cell cycle arrest mediated by miR-340. Collectively, our results demonstrate that miR-340 plays a tumor-suppressive role in glioblastoma and may be useful as a diagnostic biomarker and/or a therapeutic avenue for glioblastoma. - Highlights: • miR-340 is downregulated in glioblastoma samples and cell lines. • miR-340 inhibits glioblastoma cell proliferation. • miR-340 directly targets CDK6, cyclin-D1, and cyclin-D2. • miR-340 regulates glioblastoma cell proliferation via CDK6, cyclin-D1 and cyclin-D2.

  16. Improved Oil Recovery from Upper Jurassic Smackover Carbonates through the Application of Advanced Technologies at Womack Hill Oil Field, Choctaw and Clarke Counties, Eastern Gulf Coastal Plain

    SciTech Connect

    Ernest A. Mancini

    2003-12-31

    Pruet Production Co. and the Center for Sedimentary Basin Studies at the University of Alabama, in cooperation with Texas A&M University, Mississippi State University, University of Mississippi, and Wayne Stafford and Associates proposed a three-phase, focused, comprehensive, integrated and multidisciplinary study of Upper Jurassic Smackover carbonates (Class II Reservoir), involving reservoir characterization and 3-D modeling (Phase I) and a field demonstration project (Phases II and III) at Womack Hill Field Unit, Choctaw and Clarke Counties, Alabama, eastern Gulf Coastal Plain. Phase I of the project has been completed. The principal objectives of the project are: increasing the productivity and profitability of the Womack Hill Field Unit, thereby extending the economic life of this Class II Reservoir and transferring effectively and in a timely manner the knowledge gained and technology developed from this project to producers who are operating other domestic fields with Class II Reservoirs. The major tasks of the project included reservoir characterization, recovery technology analysis, recovery technology evaluation, and the decision to implement a demonstration project. Reservoir characterization consisted of geoscientific reservoir characterization, petrophysical and engineering property characterization, microbial characterization, and integration of the characterization data. Recovery technology analysis included 3-D geologic modeling, reservoir simulation, and microbial core experiments. Recovery technology evaluation consisted of acquiring and evaluating new high quality 2-D seismic data, evaluating the existing pressure maintenance project in the Womack Hill Field Unit, and evaluating the concept of an immobilized enzyme technology project for the Womack Hill Field Unit. The decision to implement a demonstration project essentially resulted in the decision on whether to conduct an infill drilling project in Womack Hill Field. Reservoir performance

  17. Improved Oil Recovery from Upper Jurassic Smackover Carbonates through the Application of Advanced Technologies at Womack Hill Oil Field, Choctaw and Clarke Counties, Eastern Gulf Costal Plain

    SciTech Connect

    Ernest A. Mancini

    2006-05-31

    Pruet Production Co. and the Center for Sedimentary Basin Studies at the University of Alabama, in cooperation with Texas A&M University, Mississippi State University, University of Mississippi, and Wayne Stafford and Associates proposed a three-phase, focused, comprehensive, integrated and multidisciplinary study of Upper Jurassic Smackover carbonates (Class II Reservoir), involving reservoir characterization and 3-D modeling (Phase I) and a field demonstration project (Phases II and III) at Womack Hill Field Unit, Choctaw and Clarke Counties, Alabama, eastern Gulf Coastal Plain. Phase I of the project has been completed. The principal objectives of the project are: increasing the productivity and profitability of the Womack Hill Field Unit, thereby extending the economic life of this Class II Reservoir and transferring effectively and in a timely manner the knowledge gained and technology developed from this project to producers who are operating other domestic fields with Class II Reservoirs. The major tasks of the project included reservoir characterization, recovery technology analysis, recovery technology evaluation, and the decision to implement a demonstration project. Reservoir characterization consisted of geoscientific reservoir characterization, petrophysical and engineering property characterization, microbial characterization, and integration of the characterization data. Recovery technology analysis included 3-D geologic modeling, reservoir simulation, and microbial core experiments. Recovery technology evaluation consisted of acquiring and evaluating new high quality 2-D seismic data, evaluating the existing pressure maintenance project in the Womack Hill Field Unit, and evaluating the concept of an immobilized enzyme technology project for the Womack Hill Field Unit. The decision to implement a demonstration project essentially resulted in the decision on whether to conduct an infill drilling project in Womack Hill Field. Reservoir performance

  18. HIA 2015 DOE Zero Energy Ready Home Case Study: KB Home, Double ZeroHouse 3.0, El Dorado Hill, CA

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    3.0 El Dorado Hills, CA DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced technologies are designed in to give you

  19. Hill_1974.pdf

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

  20. ,"Sault St Marie, MI Natural Gas Pipeline Exports to Canada (Million Cubic Feet)"

    Energy Information Administration (EIA) (indexed site)

    Sault St Marie, MI Natural Gas Pipeline Exports to Canada (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","Sault St Marie, MI Natural Gas Pipeline Exports to Canada (Million Cubic Feet)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release

  1. miR-206 is down-regulated in breast cancer and inhibits cell proliferation through the up-regulation of cyclinD2

    SciTech Connect

    Zhou, Jing; Tian, Ye; Li, Juan; Lu, Binbin; Sun, Ming; Zou, Yanfen; Kong, Rong; Luo, Yanhong; Shi, Yongguo; Wang, Keming; Ji, Guozhong

    2013-04-05

    Highlights: ? miR-206 was downexpressed in tumor samples compared with matched normal samples. ? Enhanced expression of miR-206 could inhibit breast cancer growth in vitro. ? Luciferase confirmed miR-206 functions as an anti-oncogene by targeting cyclinD2. ? A reverse correlation between miR-206 and cyclinD2 in breast cancer was found. -- Abstract: MicroRNAs act as important gene regulators in human genomes, and their aberrant expression is linked to many malignancies. Aberrant expression of miR-206 has been frequently reported in cancer studies; however, the role and mechanism of its function in breast cancer remains unclear. Quantitative real-time PCR was performed to detect the relative expression levels of miR-206 in breast cancer and normal breast tissues. Lower expression of miR-206 in breast cancer tissues was associated with larger tumour size and a more advanced clinical stage. Further in vitro observations showed that the enforced expression of miR-206 in MCF-7 breast cancer cells inhibited cell growth by blocking the G1/S transition and suppressed cell proliferation and colony formation, implying that miR-206 functions as a tumour suppressor in the progression of breast cancer. Interestingly, Luciferase assays first revealed that miR-206 inhibited cyclinD2 expression by targeting two binding sites in the 3?-untranslated region of cyclinD2 mRNA. qRT-PCR and Western blot assays verified that miR-206 reduced cyclinD2 expression at both the mRNA and protein levels. A reverse correlation between miR-206 and cyclinD2 expression was noted in breast cancer tissues. Altogether, our results identify a crucial tumour suppressive role of miR-206 in the progression of breast cancer, at least partly via up-regulation of the expression of cyclinD2, and suggest that miR-206 might be a candidate prognostic predictor or an anticancer therapeutic target for breast cancer patients.

  2. MiR-138 promotes smooth muscle cells proliferation and migration in db/db mice through down-regulation of SIRT1

    SciTech Connect

    Xu, Juan; Li, Li; Yun, Hui-fang; Han, Ye-shan

    2015-08-07

    Background: Diabetic vascular smooth muscle cells (VSMCs) exhibit significantly increased rates of proliferation and migration, which was the most common pathological change in atherosclerosis. In addition, the study about the role for miRNAs in the regulation of VSMC proliferation is just beginning to emerge and additional miRNAs involved in VSMC proliferation modulation should be identified. Methods: The expression of miR-138 and SIRT1 were examined in SMCs separated from db/db mice and in SMC lines C-12511 exposed to high glucose with qRT-PCR and western blot. The regulation of miR-138 on the expression of SMCs was detected with luciferase report assay. VSMCs proliferation and migration assays were performed to examine the effect of miR-138 inhibitor on VSMCs proliferation and migration. Results: We discovered that higher mRNA level of miR-138 and reduced expression of SIRT1 were observed in SMCs separated from db/db mice and in SMC lines C-12511. Moreover, luciferase report assay showed that the activity of SIRT1 3′-UTR was highly increased by miR-138 inhibitor and reduced by miR-138 mimic. In addition, we examined that the up-regulation of NF-κB induced by high glucose in SMCs was reversed by resveratrol and miR-138 inhibitor. MTT and migration assays showed that miR-138 inhibitor attenuated the proliferation and migration of smooth muscle cells. Conclusion: In this study, we revealed that miR-138 might promote proliferation and migration of SMC in db/db mice through suppressing the expression of SIRT1. - Highlights: • Higher mRNA level of miR-138 was observed in SMCs from db/db mice. • The mRNA and protein level of SIRT1 in SMCs from db/db mice were greatly reduced. • miR-138 could regulate the expression of SIRT1 in SMCs. • SIRT1 overexpression reversed the up-regulation of acetylized p65 and NF-κB induced by high glucose. • MiR-138 inhibitor reversed VSMCs proliferation and migration induced by high glucose.

  3. Report on inspection of concerns regarding DOE`s evaluation of Chevron USA`s unsolicited proposal for the Elk Hills Naval Petroleum Reserve

    SciTech Connect

    1997-11-17

    An allegation was made to the Office of Inspector General (OIG) that the integrity of the Department of Energy`s (DOE) unsolicited proposal review process may have been compromised by the actions of a former Deputy Secretary of Energy and his Executive Assistant during the review of an unsolicited proposal received from Chevron U.S.A. Production Company (Chevron) in may 1993. The Chevron unsolicited proposal was for the management and operation of DOE`s Elk Hills Naval Petroleum Reserve (Elk Hills), located near Bakersfield, California. Chevron submitted the unsolicited proposal on May 19, 1993. DOE formally rejected Chevron`s unsolicited proposal in May 1995. Although Chevron`s unsolicited proposal was eventually rejected by DOE, the complainant specifically alleged that the {open_quotes}sanctity, integrity, and sensitivity{close_quotes} of the unsolicited proposal review process had been breached in meetings during the Fall of 1993 between Chevron officials, the Deputy Secretary of Energy (Deputy Secretary), and his Executive Assistant. Based on our review of the allegation, we identified the following issue as the focus of our inspection.

  4. miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer Cells by Targeting the TLR1/NF-κB Signaling Pathway

    SciTech Connect

    Lan, Fengming; Yue, Xiao; Ren, Gang; Li, Hongqi; Ping, Li; Wang, Yingjie; Xia, Tingyi

    2015-01-01

    Purpose: Many miRNAs have been identified as essential issues and core determining factors in tumor radiation. Recent reports have demonstrated that miRNAs and Toll-like receptors could exert reciprocal effects to control cancer development in various ways. However, a novel role of miR-15a/16 in enhancing radiation sensitivity by directly targeting TLR1 has not been reported, to our knowledge. Methods and Materials: Bioinformatic analyses, luciferase reporter assay, biochemical assays, and subcutaneous tumor establishment were used to characterize the signaling pathways of miRNA-15a/16 in response to radiation treatment. Results: First, an inverse correlation between the expression of miR-15a/16 and TLR1 protein was revealed in non-small cell lung cancer (NSCLC) and normal lung tissues. Next, we corroborated that miR-15a/16 specifically bound to TLR1 3′UTR and inhibited the expression of TLR1 in H358 and A549 cells. Furthermore, miR-15a/16 downregulated the activity of the NF-κB signaling pathway through TLR1. In addition, overexpression of miR-15a/16 inhibited survival capability and increased radiation-induced apoptosis, resulting in enhancement of radiosensitivity in H358 and A549 cells. Finally, subcutaneous tumor bearing NSCLC cells in a nude mice model was established, and the results showed that combined groups (miR-15a/16 + radiation) inhibited tumor growth more significantly than did radiation alone. Conclusions: We mainly elucidate that miRNA-15a/16 can enhance radiation sensitivity by regulating the TLR1/NF-κB signaling pathway and act as a potential therapeutic approach to overcome radioresistance for lung cancer treatment.

  5. St. Clair, MI Natural Gas Pipeline Imports From Canada (Million Cubic Feet)

    Energy Information Administration (EIA) (indexed site)

    Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 14,132 11,855 34,592 33,388 2000's 17,198 21,747 28,441 5,202 22,853 18,281 10,410 9,633 9,104 6,544 2010's 5,591 5,228 3,531 6,019 16,409 9,024 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Natural Gas Pipeline Imports by Point of Entry St. Clair, MI Natural

  6. Description and crystal structure of nyholmite, a new mineral related to hureaulite, from Broken Hill, New South Wales, Australia

    SciTech Connect

    Elliott, P.; Turner, P.; Jensen, P.; Kolitsch, U.; Pring, A.

    2010-08-12

    Nyholmite, Cd{sub 3}Zn{sub 2}(AsO{sub 3}OH){sub 2}(AsO{sub 4}){sub 2} {center_dot} 4H{sub 2}O, from the Block 14 Opencut, Broken Hill, New South Wales, Australia, is a new Cd-Zn arsenate species, isostructural with the minerals of the hureaulite group. The mineral occurs in a quartz-garnet-arsenopyrite matrix as white globules, tufted aggregates of fibrous crystals and radiating hemispheres of thin, colourless, bladed crystals. Associated minerals are goldquarryite, lavendulan-sampleite, scorodite-strengite and gypsum. Individual crystals are up to 0.2 mm in length and 0.05 mm across. The mineral is transparent to translucent with a vitreous lustre. It is brittle with an uneven fracture and a white streak. The Mohs hardness is 3-3.5 and the calculated density is 4.23 g cm{sup -3} for the empirical formula. Electron microprobe analyses yielded CdO 34.58, ZnO 9.72, MnO 3.59, CuO 3.39, Al{sub 2}O{sub 3} 0.20, CaO 0.16, PbO 0.37, As{sub 2}O{sub 5} 34.55, P{sub 2}O{sub 5} 6.29 totalling 92.85 wt.%. The empirical formula, based on 20 oxygen atoms, is Ca{sub 0.03}Pb{sub 0.02} Cd{sub 2.80}Al{sub 0.04}Zn{sub 1.24}-Cu{sub 0.44}Mn{sub 0.53}[(AsO{sub 4}){sub 3.13}(PO{sub 4}){sub 0.92}]{Sigma}{sub 4.05}H{sub 1.91} {center_dot} 3.79H{sub 2}O. Nyholmite is monoclinic, C2/c, a = 18.062(4) {angstrom}, b = 9.341(2) {angstrom}, c = 9.844(2) {angstrom}, {beta} = 96.17(3){sup o}, V = 1651.2(6) {angstrom}{sup 3} (single-crystal data, at 123 K). The six strongest lines in the X-ray powder diffraction pattern are [d({angstrom}),I,(hkl)]: 8.985,30,(200); 8.283, 85,(110); 6.169,25,(111); 4.878,25,(002); 3.234,100,(222, 420); 3.079,65,(222, 511); 2.976,45,(113). The crystal structure was solved by Patterson methods and refined using 2045 observed reflections to R1(F) = 3.73%. The structure is characterized by a kinked, five-membered chain of edge-sharing M{phi}{sub 6} ({phi} = unspecified anion) octahedra, or pentamer, that extends in the a direction. The pentamers link by sharing corners to

  7. Observation of Disappearance of Muon Neutrinos in the NuMI Beam

    SciTech Connect

    Pavlovic, Zarko

    2008-05-01

    The Main Injector Neutrino Oscillation Search (MINOS) is a two detector long-baseline neutrino experiment designed to study the disappearance of muon neutrinos. MINOS will test the vμ → vτ oscillation hypothesis and measure precisely Δm232 and sin223 oscillation parameters. The source of neutrinos for MINOS experiment is Fermilab's Neutrinos at the Main Injector (NuMI) beamline. The energy spectrum and the composition of the beam is measured at two locations, one close to the source and the other 735 km down-stream in the Soudan Mine Underground Laboratory in northern Minnesota. The precision measurement of the oscillation parameters requires an accurate prediction of the neutrino flux at the Far Detector. This thesis discusses the calculation of the neutrino flux at the Far Detector and its uncertainties. A technique that uses the Near Detector data to constrain the uncertainties in the calculation of the flux is described. The data corresponding to an exposure of 2.5 x 1020 protons on the NuMI target is presented and an energy dependent disappearance pattern predicted by neutrino oscillation hypotheses is observed in the Far Detector data. The fit to MINOS data, for given exposure, yields the best fit values for Δm$2\\atop{23}$ and sin223 to be (2.38$+0.20\\atop{-0.16}$) x 10-3 eV2/c4and 1.00-0.08, respectively.

  8. Validation of the MCNPX-PoliMi Code to Design a Fast-Neutron Multiplicity Counter

    SciTech Connect

    J. L. Dolan; A. C. Kaplan; M. Flaska; S. A. Pozzi; D. L. Chichester

    2012-07-01

    Many safeguards measurement systems used at nuclear facilities, both domestically and internationally, rely on He-3 detectors and well established mathematical equations to interpret coincidence and multiplicity-type measurements for verifying quantities of special nuclear material. Due to resource shortages alternatives to these existing He-3 based systems are being sought. Work is also underway to broaden the capabilities of these types of measurement systems in order to improve current multiplicity analysis techniques. As a part of a Material Protection, Accounting, and Control Technology (MPACT) project within the U.S. Department of Energy's Fuel Cycle Technology Program we are designing a fast-neutron multiplicity counter with organic liquid scintillators to quantify important quantities such as plutonium mass. We are also examining the potential benefits of using fast-neutron detectors for multiplicity analysis of advanced fuels in comparison with He-3 detectors and testing the performance of such designs. The designs are being developed and optimized using the MCNPX-PoliMi transport code to study detector response. In the full paper, we will discuss validation measurements used to justify the use of the MCNPX-PoliMi code paired with the MPPost multiplicity routine to design a fast neutron multiplicity counter with liquid scintillators. This multiplicity counter will be designed with the end goal of safeguarding advanced nuclear fuels. With improved timing qualities associated with liquid scintillation detectors, we can design a system that is less limited by nuclear materials of high activities. Initial testing of the designed system with nuclear fuels will take place at Idaho National Laboratory in a later stage of this collaboration.

  9. T-1025 IU SciBath-768 detector tests in MI-12

    SciTech Connect

    Tayloe, Rex; Cooper, R.; Garrison, L.; Thornton, T.; Rebenitsch, L.; DeJongh, Fritz; Loer, Benjamin; Ramberg, Erik; Yoo, Jonghee; /Fermilab

    2012-02-11

    This is a memorandum of understanding between the Fermi National Accelerator Laboratory (Fermilab) and the experimenters of Department of Physics and Center for Exploration of Energy and Matter, Indiana University, who have committed to participate in detector tests to be carried out during the 2012 Fermilab Neutrino program. The memorandum is intended solely for the purpose of recording expectations for budget estimates and work allocations for Fermilab, the funding agencies and the participating institutions. it reflects an arrangement that currently is satisfactory to the parties; however, it is recognized and anticipated that changing circumstances of the evolving research program will necessitate revisions. The parties agree to modify this memorandum to reflect such required adjustments. Actual contractual obligations will be set forth in separate documents. The experimenters propsoe to test their prototype 'SciBat-768' detector in the MI-12 building for 3 months (February-April) in Spring 2012. The major goal of this effort is to measure or limit the flux of beam-induced neutrons in a far-off-axis (> 45{sup o}) location of the Booster Neutrino Beamline (BNB). This flux is of interest for a proposed coherent neutral-current neutrino-argon elastic scattering experiment. A second goal is to collect more test data for the SciBath-768 to enable better understanding and calibration of the device. The SciBath-768 detector successfully ran for 3 months in the MINOS Underground Area in Fall 2011 as testbeam experiment T-1014 and is currently running above ground in the MINOS service building. For the run proposed here, the experiments are requesting: space in MI-12 in which to run the SciBath detector during February-April 2012 while the BNB is operating; technical support to help with moving the equipment on site; access to power, internet, and accelerator signals; and a small office space from which to run and monitor the experiment.

  10. MiR-520b suppresses proliferation of hepatoma cells through targeting ten-eleven translocation 1 (TET1) mRNA

    SciTech Connect

    Zhang, Weiying; Lu, Zhanping; Gao, Yuen; Ye, Lihong; Song, Tianqiang; Zhang, Xiaodong

    2015-05-08

    Accumulating evidence indicates that microRNAs are able to act as oncogenes or tumor suppressor genes in human cancer. We previously reported that miR-520b was down-regulated in hepatocellular carcinoma (HCC) and its deregulation was involved in hepatocarcinogenesis. In the present study, we report that miR-520b suppresses cell proliferation in HCC through targeting the ten-eleven translocation 1 (TET1) mRNA. Notably, we identified that miR-520b was able to target 3′-untranslated region (3′UTR) of TET1 mRNA by luciferase reporter gene assays. Then, we revealed that miR-520b was able to reduce the expression of TET1 at the levels of mRNA and protein using reverse transcription-polymerase chain reaction and Western blotting analysis. In terms of function, 5-ethynyl-2-deoxyuridine (EdU) incorporation and colony formation assays demonstrated that the forced miR-520b expression remarkably inhibited proliferation of hepatoma cells, but TET1 overexpression could rescue the inhibition of cell proliferation mediated by miR-520b. Furthermore, anti-miR-520b enhanced proliferation of hepatoma cells, whereas silencing of TET1 abolished anti-miR-520b-induced acceleration of cell proliferation. Then, we validated that the expression levels of miR-520b were negatively related to those of TET1 mRNA in clinical HCC tissues. Thus, we conclude that miR-520b depresses proliferation of liver cancer cells through targeting 3′UTR of TET1 mRNA. Our finding provides new insights into the mechanism of hepatocarcinogenesis. - Highlights: • TET1 is a novel target gene of miR-520b. • TET1 is upregulated in clinical HCC tissues. • MiR-520b is negatively correlated with TET1 in clinical HCC tissues. • MiR-520b depresses the proliferation of HCC cells through targeting TET1 mRNA.

  11. Improved Oil Recovery from Upper Jurassic Smackover Carbonates through the Application of Advanced Technologies at Womack Hill Oil Field, Choctaw and Clarke Counties, Eastern Gulf Coastal Plain, Class II

    SciTech Connect

    Mancini, Ernest, A.; Crate, David; Blasingame, Thomas; Major, R.P.; Brown, Lewis; Stafford, Wayne

    2002-11-02

    The principal objectives of the project were: increasing the productivity and profitability of the Womack Hill Field Unit, thereby extending the economic life of this Class II Reservoir and transferring effectively and in a timely manner the knowledge gained and technology developed from this project to producers who are operating other domestic fields with Class II Reservoirs.

  12. Review of mineral estate of the United States at Naval Petroleum Reserve No. 2, Buena Vista Hills Field, Kern County, California

    SciTech Connect

    1996-08-09

    The purpose of this report is to present this Consultant`s findings regarding the nature and extent of the mineral estate of the United States at National Petroleum Reserve No. 2 (NPR-2), Buena Vista Hills Field, Kern County, California. Determination of the mineral estate is a necessary prerequisite to this Consultant`s calculation of estimated future cash flows attributable to said estate, which calculations are presented in the accompanying report entitled ``Phase II Final Report, Study of Alternatives for Future Operations of the Naval Petroleum and Oil Shale Reserves, NPR-2, California.`` This Report contains a discussion of the leases in effect at NPR-2 and subsequent contracts affecting such leases. This Report also summarizes discrepancies found between the current royalty calculation procedures utilized at NPR-2 and those procedures required under applicable agreements and regulations. Recommendations for maximizing the government`s income stream at NPR-2 are discussed in the concluding section of this Report.

  13. The use of wireline pressure measurements to refine reservoir description, Main Body B waterflood, Elk Hills oil field, Kern County, California

    SciTech Connect

    Wilson, M. ); Love, C. ); Fishburn, M. ); Humphrey, M. )

    1991-02-01

    The Main Body B, one of five large Stevens sand reservoirs at Elk Hills, occupies the eastern half of the 31S anticline. Early in the production history of this reservoir, the Elk Hills unit initiated peripheral water injection to maintain reservoir pressure. Water injection has proceeded at a rate approximately equal to the voidage created by oil and gas production and has moved water upstructure creating an oil bank. Bechtel Petroleum Operations Inc., the current unit operator, drills five to ten new wells each year to fully exploit this oil bank. In 1985, the unit added wireline pressure measurements to the open-hole logging programs of these infill wells for the purpose of evaluating the net effect of injection into and production from the Main Body B reservoir. A typical well provides the opportunity to obtain 8-10 pressures from the Main Body B. To date, the Unit has measured wireline pressures in more than two dozen wells. The wireline measurements have shown a broader than expected range of formation pressures (1,600 {plus minus} psi to 4,200 {plus minus} psi). The pressures show that this is a layered reservoir with little vertical pressure communication between some of the layers. In some parts of the reservoir, wireline pressures indicate horizontal continuity of the layers between wells and in other areas pressure differences between adjacent wells may indicate faults or cementation barriers. Permeabilities calculated from the sampling drawdown are the same order of magnitude as brine permeabilities obtained from core and show that higher-pressured layers of the reservoir have lower permeability. These observations fundamentally alter performance evaluation of the Main Body B waterflood.

  14. The RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a

    SciTech Connect

    Hu, Cheng-En; Liu, Yong-Chao; Zhang, Hui-Dong; Huang, Guang-Jian

    2014-06-13

    Highlights: • PCBP2 is overexpressed in human gastric cancer. • PCBP2 high expression predicts poor survival. • PCBP2 regulates gastric cancer growth in vitro and in vivo. • PCBP2 regulates gastric cancer apoptosis by targeting miR-34a. - Abstract: Gastric carcinoma is the fourth most common cancer worldwide, with a high rate of death and low 5-year survival rate. However, the mechanism underling gastric cancer is still not fully understood. Here in the present study, we identify the RNA-binding protein PCBP2 as an oncogenic protein in human gastric carcinoma. Our results show that PCBP2 is up-regulated in human gastric cancer tissues compared to adjacent normal tissues, and that high level of PCBP2 predicts poor overall and disease-free survival. Knockdown of PCBP2 in gastric cancer cells inhibits cell proliferation and colony formation in vitro, whereas opposing results are obtained when PCBP2 is overexpressed. Our in vivo subcutaneous xenograft results also show that PCBP2 can critically regulate gastric cancer cell growth. In addition, we find that PCBP2-depletion induces apoptosis in gastric cancer cells via up-regulating expression of pro-apoptotic proteins and down-regulating anti-apoptotic proteins. Mechanically, we identify that miR-34a as a target of PCBP2, and that miR-34a is critically essential for the function of PCBP2. In summary, PCBP2 promotes gastric carcinoma development by regulating the level of miR-34a.

  15. Ginsenoside-Rg{sub 1} induces angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a

    SciTech Connect

    Kwok, Hoi-Hin; Chan, Lai-Sheung; Poon, Po-Ying; Yue, Patrick Ying-Kit; Wong, Ricky Ngok-Shun

    2015-09-15

    Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. Ginsenoside-Rg{sub 1} (Rg{sub 1}), one of the most abundant active components of ginseng, has been demonstrated as an angiogenesis-stimulating compound in different models. There is increasing evidence implicating microRNAs (miRNAs), a group of non-coding RNAs, as important regulators of angiogenesis, but the role of microRNAs in Rg{sub 1}-induced angiogenesis has not been fully explored. In this report, we found that stimulating endothelial cells with Rg{sub 1} could reduce miR-23a expression. In silico experiments predicted hepatocyte growth factor receptor (MET), a well-established mediator of angiogenesis, as the target of miR-23a. Transfection of the miR-23a precursor or inhibitor oligonucleotides validated the inverse relationship of miR-23a and MET expression. Luciferase reporter assays further confirmed the interaction between miR-23a and the MET mRNA 3′-UTR. Intriguingly, ginsenoside-Rg{sub 1} was found to increase MET protein expression in a time-dependent manner. We further demonstrated that ginsenoside-Rg{sub 1}-induced angiogenic activities were indeed mediated through the down-regulation of miR-23a and subsequent up-regulation of MET protein expression, as confirmed by gain- and loss-of-function angiogenic experiments. In summary, our results demonstrated that ginsenoside-Rg{sub 1} could induce angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a. This study has broadened our understanding of the non-genomic effects of ginsenoside-Rg{sub 1,} and provided molecular evidence that warrant further development of natural compound as novel angiogenesis-promoting therapy. - Highlights: • Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. • Ginsenoside-Rg{sub 1} (Rg{sub 1}) has been demonstrated as an angiogenesis-stimulating compound. • We found that Rg{sub 1} induces angiogenesis by

  16. Analysis of the hydraulic data from the MI fracture zone at the Grimsel Rock Laboratory, Switzerland

    SciTech Connect

    Davey, A.; Karasaki, K.; Long, J.C.S.; Landsfeld, M.; Mensch, A.; Martel, S.J.

    1989-10-01

    One of the major problems in analyzing flow and transport in fractured rock is that the flow may be largely confined to a poorly connected network of fractures. In order to overcome some of this problem, Lawrence Berkeley Laboratory (LBL) has been developing a new type of fracture hydrology model called an equivalent discontinuum model. In this model the authors represent the discontinuous nature of the problem through flow on a partially filled lattice. A key component in constructing an equivalent discontinuum model from this lattice is removing some of the conductive elements such that the system is partially connected in the same manner as the fracture network. This is done through a statistical inverse technique called simulated annealing. The fracture network model is annealed by continually modifying a base model, or template such that the modified systems behave more and more like the observed system. In order to see how the simulated annealing algorithm works, the authors have developed a series of synthetic real cases. In these cases, the real system is completely known so that the results of annealing to steady state data can be evaluated absolutely. The effect of the starting configuration has been studied by varying the percent of conducting elements in the initial configuration. Results have shown that the final configurations converge to about the same percentage of conducting elements. An example using Nagra field data from the Migration Experiment (MI) at Grimsel Rock Laboratory in Switzerland is also analyzed. 24 refs., 33 figs., 3 tabs.

  17. A Flexible Baseline Design for the Advanced High Temperature Reactor Using Metallic Internals (AHTR-MI)

    SciTech Connect

    Peterson, Per F.; Haihua, Zhao

    2006-07-01

    The Advanced High Temperature Reactor (AHTR) is a novel reactor concept that uses clean liquid fluoride salts as a high temperature primary coolant for conventional graphite matrix, coated particle fuels. The very large volumetric heat capacity and excellent natural circulation heat transfer provided by liquid salts enables the design of high-temperature reactors with passive safety features and thermal powers ranging from 2400 to 4000 MW(t). To make optimal use of existing design information, early AHTR designs were modeled upon the S-PRISM sodium pool-reactor design, but introduced an insulated reactor vessel. Subsequent work has shown that liquid-salt cooled systems share important similarities with pressurized water reactors, which use a closed primary loop. This article discusses the use of a closed primary loop immersed in a separate buffer salt tank, which permits the primary loop components to be fabricated from metallic materials (AHTR-MI). This approach may provide several key advantages over open loop approaches, including very large thermal inertia. (authors)

  18. CX-006488: Categorical Exclusion Determination | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    488: Categorical Exclusion Determination CX-006488: Categorical Exclusion Determination Chrysler Group LLC, Revised Specific Project Application 2, Retooling, Reequipping and Engineering CX(s) Applied: B1.31, B5.1 Date: 09/06/2011 Location(s): Auburn Hills, Michigan Office(s): Advanced Technology Vehicles Manufacturing Loan Program, Loan Guarantee Program Office The Department of Energy will issue a loan to Chrysler Group, LLC. The loan would include engineering integration for the proposed

  19. Monocyte to macrophage differentiation-associated (MMD) targeted by miR-140-5p regulates tumor growth in non-small cell lung cancer

    SciTech Connect

    Li, Weina; He, Fei

    2014-07-18

    Highlights: • Expression of MMD is increased in lung cancer tissues. • Knockdown of MMD inhibits growth of A549 and LLC cells in vitro and in vivo. • MMD is a direct functional target of miR-140-5p. • MiR-140-5p/MMD axis regulates Erk1/2 signaling. - Abstract: Monocyte to macrophage differentiation-associated (MMD) is identified in macrophages as a gene associated with the differentiation from monocytes to macrophages. Recent microarray analysis for non-small cell lung cancer (NSCLC) suggests that MMD is an important signature associated with relapse and survival among patients with NSCLC. Therefore, we speculate that MMD likely plays a role in lung cancer. In this study, we found that the protein level of MMD was increased in lung cancer compared to benign lung tissues, and knockdown of MMD inhibited the growth of A549 and Lewis lung cancer cells (LLC) in vitro and in vivo. Integrated analysis demonstrated that MMD was a direct functional target of miR-140-5p. Furthermore, we found that miR-140-5p/MMD axis could affect the cell proliferation of lung cancer cells by regulating Erk signaling. Together, our results highlight the significance of miR-140-5p/MMD axis in lung cancer, and miR-140-5p/MMD axis could serve as new molecular targets for the therapy against lung cancer.

  20. Executive summary of major NuMI lessons learned: a review of relevant meetings of Fermilab's DUSEL Beamline Working Group

    SciTech Connect

    Andrews, Mike; Appel, Jeffrey A.; Bogert, Dixon; Childress, Sam; Cossairt, Don; Griffing, William; Grossman, Nancy; Harding, David; Hylen, Jim; Kuchler, Vic; Laughton, Chris; /Fermilab /Argonne /Brookhaven /LBL, Berkeley

    2009-05-01

    We have gained tremendous experience with the NuMI Project on what was a new level of neutrino beams from a high power proton source. We expect to build on that experience for any new long baseline neutrino beam. In particular, we have learned about some things which have worked well and/or where the experience is fairly directly applicable to the next project (e.g., similar civil construction issues including: tunneling, service buildings, outfitting, and potential claims/legal issues). Some things might be done very differently (e.g., decay pipe, windows, target, beam dump, and precision of power supply control/monitoring). The NuMI experience does lead to identification of critical items for any future such project, and what issues it will be important to address. The DUSEL Beamline Working Group established at Fermilab has been meeting weekly to collect and discuss information from that NuMI experience. This document attempts to assemble much of that information in one place. In this Executive Summary, we group relevant discussion of some of the major issues and lessons learned under seven categories: (1) Differences Between the NuMI Project and Any Next Project; (2) The Process of Starting Up the Project; (3) Decision and Review Processes; (4) ES&H: Environment, Safety, and Health; (5) Local Community Buy-In; (6) Transition from Project Status to Operation; and (7) Some Lessons on Technical Elements. We concentrate here on internal project management issues, including technical areas that require special attention. We cannot ignore, however, two major external management problems that plagued the NuMI project. The first problem was the top-down imposition of an unrealistic combination of scope, cost, and schedule. This situation was partially corrected by a rebaselining. However, the full, desirable scope was never achievable. The second problem was a crippling shortage of resources. Critical early design work could not be done in a timely fashion, leading to

  1. Hepatitis B virus X protein mutant HBxΔ127 promotes proliferation of hepatoma cells through up-regulating miR-215 targeting PTPRT

    SciTech Connect

    Liu, Fabao; You, Xiaona; Chi, Xiumei; Wang, Tao; Ye, Lihong; Niu, Junqi; Zhang, Xiaodong

    2014-02-07

    Highlights: • Relative to wild type HBx, HBX mutant HBxΔ127 strongly enhances cell proliferation. • Relative to wild type HBx, HBxΔ127 remarkably up-regulates miR-215 in hepatoma cells. • HBxΔ127-elevated miR-215 promotes cell proliferation via targeting PTPRT mRNA. - Abstract: The mutant of virus is a frequent event. Hepatitis B virus X protein (HBx) plays a vital role in the development of hepatocellular carcinoma (HCC). Therefore, the identification of potent mutant of HBx in hepatocarcinogenesis is significant. Previously, we identified a natural mutant of the HBx gene (termed HBxΔ127). Relative to wild type HBx, HBxΔ127 strongly enhanced cell proliferation and migration in HCC. In this study, we aim to explore the mechanism of HBxΔ127 in promotion of proliferation of hepatoma cells. Our data showed that both wild type HBx and HBxΔ127 could increase the expression of miR-215 in hepatoma HepG2 and H7402 cells. However, HBxΔ127 was able to significantly increase miR-215 expression relative to wild type HBx in the cells. We identified that protein tyrosine phosphatase, receptor type T (PTPRT) was one of the target genes of miR-215 through targeting 3′UTR of PTPRT mRNA. In function, miR-215 was able to promote the proliferation of hepatoma cells. Meanwhile anti-miR-215 could partially abolish the enhancement of cell proliferation mediated by HBxΔ127 in vitro. Knockdown of PTPRT by siRNA could distinctly suppress the decrease of cell proliferation mediated by anti-miR-215 in HepG2-XΔ127/H7402-XΔ127 cells. Moreover, we found that anti-miR-215 remarkably inhibited the tumor growth of hepatoma cells in nude mice. Collectively, relative to wild type HBx, HBxΔ127 strongly enhances proliferation of hepatoma cells through up-regulating miR-215 targeting PTPRT. Our finding provides new insights into the mechanism of HBx mutant HBxΔ127 in promotion of proliferation of hepatoma cells.

  2. Environmental Assessment and Finding of No Significant Impact: Waste Remediation Activities at Elk Hills (Former Naval petroleum Reserve No. 1), Kern County, California

    SciTech Connect

    N /A

    1999-12-17

    DOE proposes to conduct a variety of post-sale site remediation activities, such as characterization, assessment, clean-up, and formal closure, at a number of inactive waste sites located at Elk Hills. The proposed post-sale site remediation activities, which would be conducted primarily in developed portions of the oil field, currently are expected to include clean-up of three basic categories of waste sites: (1) nonhazardous solid waste surface trash scatters, (2) produced wastewater sumps, and (3) small solid waste landfills. Additionally, a limited number of other inactive waste sites, which cannot be typified under any of these three categories, have been identified as requiring remediation. Table 2.1-1 presents a summary, organized by waste site category, of the inactive waste sites that require remediation per the PSA, the ASA, and/or the UPCTA. The majority of these sites are known to contain no hazardous waste. However, one of the surface scatter sites (2G) contains an area of burn ash with hazardous levels of lead and zinc, another surface scatter site (25S) contains an area with hazardous levels of lead, a produced wastewater sump site (23S) and a landfill (42-36S) are known to contain hazardous levels of arsenic, and some sites have not yet been characterized. Furthermore, additional types of sites could be discovered. For example, given the nature of oil field operations, sites resulting from either spills or leaks of hazardous materials could be discovered. Given the nature of the agreements entered into by DOE regarding the required post-sale clean-up of the inactive waste sites at Elk Hills, the Proposed Action is the primary course of action considered in this EA. The obligatory remediation activities included in the Proposed Action are standard procedures such that possible variations of the Proposed Action would not vary substantially enough to require designation as a separate, reasonable alternative. Thus, the No Action Alternative is the only

  3. Results of sediment and water sampling for inorganic, organic, and radionuclide analysis at recreation areas and water intakes -- Norris, Melton Hill, and Watts Bar Lakes. Data report

    SciTech Connect

    1991-10-01

    Suspected water quality contamination in Watts Bar Reservoir as a result of activities in past decades at the Department of Energy`s (DOE) Oak Ridge facility is of public concern. DOE, the Tennessee Valley Authority (TVA), the State of Tennessee, and other agencies and officials have received many inquiries from the public in recent years concerning this suspected pollution, especially how this potential contamination may affect the health and safety of those persons who use beaches in the area for swimming or other water-body-contact sports. As a result of these concerns, TVA conducted a study in May and June 1991 to obtain data on potential contaminants of concern in the water and sediment of Watts Bar Reservoir. TVA collected water and sediment samples at a total of 29 sites, including 18 recreation areas and 11 water intake locations, located throughout Norris, Melton Hill, and Watts Bar Reservoirs. The samples were analyzed for radionuclides, metals, and organic compounds which could pose a threat to human health.

  4. Treatability study for Hill AFB`s Operable Unit-1: Enhanced microaerobic dechlorination using various electron donors. MasMajor report

    SciTech Connect

    Breed, P.G.

    1999-05-13

    A treatability study of the microaerobic biodegradation of cis-dichloroethene (c-DCE) was completed using a series of eight continuously operated columns filled with contaminated soils from Hill Air Force Base`s Operable Unit 1. Columns were supplied groundwater from the site, vitamins and yeast, and an electron donor solution containing one of the following donors: n-butyric acid, benzoic acid, lactic acid, propionic acid, n-propanol, or toluene. Concentrations of c-DCE varied over six months and ranged from 2736 micrograms/L to 30 micrograms/L. Though attempted as an anaerobic study, the ability to continuously eliminate oxygen from an active system proved difficult and columns operated as microaerobic systems. In all columns the degradation of c-DCE was observed, however, the removal efficiencies determined by comparing the influent and effluent concentrations were highly inconsistent throughout the experiment. By comparing the background columns to the columns supplied electron donors, it does not appear the addition of vitamins or electron donors enhance the indigenous microorganism`s ability to remove c-DCE. While c-DCE removal within the background column averaged 17%, the vitamin amended control column averaged only 7% c-DCE removal within the column and the electron donor supplied columns averaged between 7% removal and 5% apparent production. Of the electron donors supporting c-DCE removal, benzoic acid demonstrated 7% removal followed closely by propionic acid and n-propanol, both showing 5% c-DCE removal.

  5. Mitsubishi iMiEV: An Electric Mini-Car in NREL's Advanced Technology Vehicle Fleet (Fact Sheet)

    SciTech Connect

    Not Available

    2011-10-01

    This fact sheet highlights the Mitsubishi iMiEV, an electric mini-car in the advanced technology vehicle fleet at the National Renewable Energy Laboratory (NREL). In support of the U.S. Department of Energy's fast-charging research efforts, NREL engineers are conducting charge and discharge performance testing on the vehicle. NREL's advanced technology vehicle fleet features promising technologies to increase efficiency and reduce emissions without sacrificing safety or comfort. The fleet serves as a technology showcase, helping visitors learn about innovative vehicles that are available today or are in development. Vehicles in the fleet are representative of current, advanced, prototype, and emerging technologies.

  6. Improved Oil Recovery from Upper Jurassic Smackover Carbonates through the Application of Advanced Technologies at Womack Hill Oil Field, Choctaw and Clarke Counties, Alabama, Eastern Gulf Coastal Plan (Phase II)

    SciTech Connect

    Ernest A. Mancini; Joe Benson; David Hilton; David Cate; Lewis Brown

    2006-05-29

    The principal research efforts for Phase II of the project were drilling an infill well strategically located in Section 13, T. 10 N., R. 2 W., of the Womack Hill Field, Choctaw and Clarke Counties, Alabama, and obtaining fresh core from the upper Smackover reservoir to test the feasibility of implementing an immobilized enzyme technology project in this field. The Turner Land and Timber Company 13-10 No. 1 well was successfully drilled and tested at a daily rate of 132 barrels of oil in Section 13. The well has produced 27,720 barrels of oil, and is currently producing at a rate of 60 barrels of oil per day. The 13-10 well confirmed the presence of 175,000 barrels of attic (undrained) oil in Section 13. As predicted from reservoir characterization, modeling and simulation, the top of the Smackover reservoir in the 13-10 well is structurally high to the tops of the Smackover in offsetting wells, and the 13-10 well has significantly more net pay than the offsetting wells. The drilling and testing of the 13-10 well showed that the eastern part of the field continues to have a strong water drive and that there is no need to implement a pressure maintenance program in this part of the Womack Hill Field at this time. The success achieved in drilling and testing the 13-10 infill well demonstrates the benefits of building a geologic model to target areas in mature fields that have the potential to contain undrained oil, thus increasing the productivity and profitability of these fields. Microbial cultures that grew at 90 C and converted ethanol to acid were recovered from fresh cuttings from the Smackover carbonate reservoir in an analogous field to the Womack Hill Field in southwest Alabama; however, no viable microorganisms were found in the Smackover cores recovered from the drilling of the 13-10 well in Womack Hill Field. Further evaluation is, therefore, required prior to implementing an immobilized enzyme technology project in the Womack Hill Field.

  7. Recirculating industrial air: The impact on air compliance and workers. Safety case study: Hill Air Force Base C-130 painting operations

    SciTech Connect

    LaPuma, P.T.

    1998-06-29

    The 1990 Clean Air Act Amendment resulted in new environmental regulations called the National Emission Standards for Hazardous Air Pollutants (NESHAPs). Industries such as painting facilities may have to treat large volumes of air, which drives the cost of an air control system. Recirculating a portion of the air back into the facility is an option to reduce the amount of air to be treated. A guided computer model written in Microsoft Excel 97% is developed to analyze worker safety and compliance costs with a focus on recirculation. The model has a chemical database containing over 1300 chemicals and requires inputs such as tasks performed, hazardous products used, and chemical make-up of the products. The model will predict indoor air concentrations in relation to occupational exposure limits (OELs). A case study is performed on a C-130 aircraft painting facility at Hill AFB, UT. The Aerospace NESHAP requires air pollution reductions in aircraft painting operations. The model predicts strontium chromate concentrations found in primer paints will reach 1000 times the OEL. Strontium chromate and other solid particulates are nearly unaffected by recirculation because the air is filtered prior to recirculation. The next highest chemical, hexamethylene diisocyanate (HDI), increases from 2.6 to 10.5 times the OEL at 0% and 75% recirculation, respectively. Due to the level of respiratory protection required for the strontium chromate, workers are well protected from the modest increases in concentrations caused by recirculating 75%. The initial cost of a VOC control system with no recirculation is $4.5 million and $1.8 million at 75% recirculation. To decide the best operating conditions for a facility, all options such as product substitution, operational changes or recirculation should be explored. The model is an excellent tool to evaluate these options.

  8. Evaluation of Multiplexed 16S rRNA Microbial Population Surveys Using Illumina MiSeq Platform (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema

    Tremblay, Julien [DOE JGI

    2013-01-25

    Julien Tremblay from DOE JGI presents "Evaluation of Multiplexed 16S rRNA Microbial Population Surveys Using Illumina MiSeq Platorm" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  9. Aerial photographic interpretation of lineaments and faults in late Cenozoic deposits in the eastern parts of the Saline Valley 1:100, 000 quadrangle, Nevada and California, and the Darwin Hills 1:100, 000 quadrangle, California

    SciTech Connect

    Reheis, M.C.

    1991-09-01

    Faults and fault-related lineaments in Quaternary and late Tertiary deposits in the southern part of the Walker Lane are potentially active and form patterns that are anomalous compared to those in most other areas of the Great Basin. Two maps at a scale of 1:100,000 summarize information about lineaments and faults in the area around and southwest of the Death Valley-Furnace Creek fault system based on extensive aerial-photo interpretation, limited field interpretation, limited field investigations, and published geologic maps. There are three major fault zones and two principal faults in the Saline Valley and Darwin Hills 1:100,000 quadrangles. (1) The Death Valley-Furnace Creek fault system and (2) the Hunter Mountain fault zone are northwest-trending right-lateral strike-slip fault zones. (3) The Panamint Valley fault zone and associated Towne Pass and Emigrant faults are north-trending normal faults. The intersection of the Hunter Mountain and Panamint Valley fault zones is marked by a large complex of faults and lineaments on the floor of Panamint Valley. Additional major faults include (4) the north-northwest-trending Ash Hill fault on the west side of Panamint Valley, and (5) the north-trending range-front Tin Mountain fault on the west side of the northern Cottonwood Mountains. The most active faults at present include those along the Death Valley-Furnace Creek fault system, the Tin Mountain fault, the northwest and southeast ends of the Hunter Mountain fault zone, the Ash Hill fault, and the fault bounding the west side of the Panamint Range south of Hall Canyon. Several large Quaternary landslides on the west sides of the Cottonwood Mountains and the Panamint Range apparently reflect slope instability due chiefly to rapid uplift of these ranges. 16 refs.

  10. A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

    SciTech Connect

    Nagarkar-Jaiswal, Sonal; Lee, Pei-Tseng; Campbell, Megan E.; Chen, Kuchuan; Anguiano-Zarate, Stephanie; Cantu Gutierrez, Manuel; Busby, Theodore; Lin, Wen-Wen; He, Yuchun; Schulze, Karen L.; Booth, Benjamin W.; Evans-Holm, Martha; Venken, Koen J.T.; Levis, Robert W.; Spradling, Allan C.; Hoskins, Roger A.; Bellen, Hugo J.

    2015-03-31

    Here, we document a collection of ~7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.

  11. A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

    DOE PAGES [OSTI]

    Nagarkar-Jaiswal, Sonal; Lee, Pei-Tseng; Campbell, Megan E.; Chen, Kuchuan; Anguiano-Zarate, Stephanie; Cantu Gutierrez, Manuel; Busby, Theodore; Lin, Wen-Wen; He, Yuchun; Schulze, Karen L.; et al

    2015-03-31

    Here, we document a collection of ~7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstratemore » reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.« less

  12. Improved Oil Recovery from Upper Jurassic Smackover Carbonates through the Application of Advanced Technologies at Womack Hill Oil Field, Choctaw and Clarke Counties, Eastern Gulf Coastal Plain, Class II

    SciTech Connect

    Mancini, Ernest A.; Cate, David; Blasingame, Thomas; Major, R.P.; Brown, Lewis; Stafford, Wayne

    2001-08-07

    The principal objectives of this project was to: increase the productivity and profitability of the Womack Hill Field Unit, thereby extending the economic life of this Class II Reservoir and transferring effectively and in a timely manner the knowledge gained and technology developed from this project to producers who are operating other domestic fields with Class II Reservoirs. Efforts for Year 1 of this project has been reservoir characterization, which has included three (3) primary tasks: geoscientific reservoir characterization, petrophysical and engineering property characterization, and microbial characterization.

  13. AGU 2011 poster Craig Hill

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    St. Anthony Falls Laboratory, College of Science & Engineering, University of Minnesota, Minneapolis, MN 55414, USA 2 Energy-Water-Ecosystems Engineering, Wind and Water Power...

  14. Bull Hill | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    First Wind Energy Purchaser NSTAR Location Hancock County ME Coordinates 44.723076, -68.170852 Show Map Loading map... "minzoom":false,"mappingservice":"googlemaps3","type":...

  15. AGU 2011 poster Craig Hill

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    and instrumentation of the turbine power system and synchronized ADV measurement setup. ... interaction around a hydrokinetic turbine. Part I: Experiments." In preparation. ...

  16. Laurel Hill | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    W 69,000,000,000 mW 0.069 GW Number of Units 30 Commercial Online Date 2012 Wind Turbine Manufacturer Siemens References AWEA 2012 Market Report1 Loading map......

  17. Mustang Hills | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner Terra-Gen Power Developer Terra-Gen Power Energy Purchaser Southern California Edison Co Location...

  18. Science on the Hill - 2016

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    - 91216 Hot-cast perovskite solar cells show promise for creating more efficient and affordable solar panels, LEDs, and lasers. Image courtesy Los Alamos National Laboratory. ...

  19. Cedro Hill | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner Edison Mission Energy Developer DKRW Wind LLC Location Located in Bruni TX Coordinates...

  20. Repression of miR-17-5p with elevated expression of E2F-1 and c-MYC in non-metastatic hepatocellular carcinoma and enhancement of cell growth upon reversing this expression pattern

    SciTech Connect

    El Tayebi, H.M.; Omar, K.; Hegy, S.; El Maghrabi, M.; El Brolosy, M.; Hosny, K.A.; Esmat, G.; Abdelaziz, A.I.

    2013-05-10

    Highlights: The oncogenic miR-17-5p is downregulated in non-metastatic hepatocellular carcinoma patients. E2F-1 and c-MYC transcripts are upregulated in non-metastatic HCC patients. miR-17-5p forced overexpression inhibited E2F-1 and c-MYC expression in HuH-7 cells. miR-17-5p mimicking increased HuH-7 cell growth, proliferation, migration and colony formation. miR-17-5p is responsible for HCC progression among the c-MYC/E2F-1/miR-17-5p triad members. -- Abstract: E2F-1, c-MYC, and miR-17-5p is a triad of two regulatory loops: a negative and a positive loop, where c-MYC induces the expression of E2F-1 that induces the expression of miR-17-5p which in turn reverses the expression of E2F-1 to close the loop. In this study, we investigated this triad for the first time in hepatocellular carcinoma (HCC), where miR-17-5p showed a significant down-regulation in 23 non-metastatic HCC biopsies compared to 10 healthy tissues; however, E2F-1 and c-MYC transcripts were markedly elevated. Forced over-expression of miR-17-5p in HuH-7 cells resulted in enhanced cell proliferation, growth, migration and clonogenicity with concomitant inhibition of E2F-1 and c-MYC transcripts expressions, while antagomirs of miR-17-5p reversed these events. In conclusion, this study revealed a unique pattern of expression for miR-17-5p in non-metastatic HCC patients in contrast to metastatic HCC patients. In addition we show that miR-17-5p is the key player among the triad that tumor growth and spread.