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Study of the hydration of globular proteins by broad NMR lines method

Conference:

Abstract

Spectra of proteins and polypeptides obtained by means of a NMR broad line spectrometer consist of broad and thin lines. These broad and thin lines are attributed to proteins and to water absorbed on the surfaces of proteins respectively. The behaviour of the thin line in the spectra of lyophilizated albumin of the egg white has been studied in the temperature range from -42 to 20/sup 0/C. The amount of water has been found by the simple method of weighing and has been equal about 7% of the total weight. It has been found that the water absorbed on the surface of the lyophilizated proteins gives a thinner line in comparison to the water absorbed on molecules of proteins in water solutions and that the correlation time is about 10/sup 3/ times greater.
Authors:
Blicharska, B [1] 
  1. Uniwersytet Jagiellonski, Krakow (Poland). Instytut Fizyki
Publication Date:
Jan 01, 1973
Product Type:
Conference
Report Number:
INP-819/PL
Reference Number:
AIX-08-291297; EDB-77-094900
Resource Relation:
Conference: 5. All-Polish seminar on nuclear magnetic resonance and its applications, Krakow, Poland, 1 Dec 1972; Related Information: In: Proceedings of the fifth all-Polish seminar on nuclear magnetic resonance and its applications. Vol. 1.
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; ALBUMINS; HYDRATION; PROTEINS; CORRELATION FUNCTIONS; GAUSS FUNCTION; LINE WIDTHS; NUCLEAR MAGNETIC RESONANCE; SPECTRA; TEMPERATURE DEPENDENCE; USES; WATER; FUNCTIONS; HYDROGEN COMPOUNDS; MAGNETIC RESONANCE; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; RESONANCE; SOLVATION; 400301* - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)
OSTI ID:
8569014
Research Organizations:
Institute of Nuclear Physics, Krakow (Poland)
Country of Origin:
Poland
Language:
Polish
Availability:
INIS
Submitting Site:
INIS
Size:
Pages: 105-109
Announcement Date:
Mar 01, 1977

Conference:

Citation Formats

Blicharska, B. Study of the hydration of globular proteins by broad NMR lines method. Poland: N. p., 1973. Web.
Blicharska, B. Study of the hydration of globular proteins by broad NMR lines method. Poland.
Blicharska, B. 1973. "Study of the hydration of globular proteins by broad NMR lines method." Poland.
@misc{etde_8569014,
title = {Study of the hydration of globular proteins by broad NMR lines method}
author = {Blicharska, B}
abstractNote = {Spectra of proteins and polypeptides obtained by means of a NMR broad line spectrometer consist of broad and thin lines. These broad and thin lines are attributed to proteins and to water absorbed on the surfaces of proteins respectively. The behaviour of the thin line in the spectra of lyophilizated albumin of the egg white has been studied in the temperature range from -42 to 20/sup 0/C. The amount of water has been found by the simple method of weighing and has been equal about 7% of the total weight. It has been found that the water absorbed on the surface of the lyophilizated proteins gives a thinner line in comparison to the water absorbed on molecules of proteins in water solutions and that the correlation time is about 10/sup 3/ times greater.}
place = {Poland}
year = {1973}
month = {Jan}
}