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Effect of two organophosphorus insecticides on the growth, respiration and (/sup 14/C)-glucose metabolism of Azobacter chroococcum Beij

Abstract

The two organophosphorus insecticides, commonly applied to soil, viz., disulfoton (0,0-diethyl S-2-ethyl thio ethyl phosphorodithioate) and fensulfothion (0,0-diethyl 0-4-methyl sulphinyl phenyl phosphorothioate) did not affect the in vitro growth of Azotobacter chroococcum Beij., the free-living, nitrogen fixing soil bacterium, at 2 ppm (lower level), while the normal dose (5 ppm) and the higher level (10 ppm) suppressed the growth. Respiration of the organism (glucose oxidation) was adversely affected by the insecticides in the growth medium and the inhibition increased with the concentration of the chemical. Both the insecticides suppressed the assimilation of (/sup 14/C)-glucose in the cold-TCA soluble, hot-TCA soluble fractions and insoluble residue of the cells whereas the /sup 14/C-incorporation in the alcohol soluble and alcohol-ether soluble fractions was enhanced indicating that the insecticides considerably altered the glucose metabolism of the bacterium.
Authors:
Balasubramanian, A; Narayanan, R [1] 
  1. Tamil Nadu Agricultural Univ., Coimbatore (India)
Publication Date:
Jan 01, 1980
Product Type:
Conference
Report Number:
CONF-780299-
Reference Number:
AIX-13-714074; EDB-83-078785
Resource Relation:
Conference: Symposium on nuclear techniques in studies of metabolism effects and degradation of pesticides, Tirupati, India, 2 Feb 1978; Related Information: In: Proceedings of the symposium on nuclear techniques in studies of metabolism, effects and degradation of pesticides (held at) Tirupati, February 2-4, 1978.
Subject:
59 BASIC BIOLOGICAL SCIENCES; AZOTOBACTER; INSECTICIDES; METABOLISM; CARBON 14 COMPOUNDS; GLUCOSE; GROWTH; IN VITRO; LABELLED COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; RESPIRATION; SOILS; TRACER TECHNIQUES; ALDEHYDES; BACTERIA; CARBOHYDRATES; HEXOSES; ISOTOPE APPLICATIONS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PESTICIDES; SACCHARIDES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
8418839
Country of Origin:
India
Language:
English
Submitting Site:
INIS
Size:
Pages: 169-179
Announcement Date:
Nov 01, 1982

Citation Formats

Balasubramanian, A, and Narayanan, R. Effect of two organophosphorus insecticides on the growth, respiration and (/sup 14/C)-glucose metabolism of Azobacter chroococcum Beij. India: N. p., 1980. Web.
Balasubramanian, A, & Narayanan, R. Effect of two organophosphorus insecticides on the growth, respiration and (/sup 14/C)-glucose metabolism of Azobacter chroococcum Beij. India.
Balasubramanian, A, and Narayanan, R. 1980. "Effect of two organophosphorus insecticides on the growth, respiration and (/sup 14/C)-glucose metabolism of Azobacter chroococcum Beij." India.
@misc{etde_8418839,
title = {Effect of two organophosphorus insecticides on the growth, respiration and (/sup 14/C)-glucose metabolism of Azobacter chroococcum Beij}
author = {Balasubramanian, A, and Narayanan, R}
abstractNote = {The two organophosphorus insecticides, commonly applied to soil, viz., disulfoton (0,0-diethyl S-2-ethyl thio ethyl phosphorodithioate) and fensulfothion (0,0-diethyl 0-4-methyl sulphinyl phenyl phosphorothioate) did not affect the in vitro growth of Azotobacter chroococcum Beij., the free-living, nitrogen fixing soil bacterium, at 2 ppm (lower level), while the normal dose (5 ppm) and the higher level (10 ppm) suppressed the growth. Respiration of the organism (glucose oxidation) was adversely affected by the insecticides in the growth medium and the inhibition increased with the concentration of the chemical. Both the insecticides suppressed the assimilation of (/sup 14/C)-glucose in the cold-TCA soluble, hot-TCA soluble fractions and insoluble residue of the cells whereas the /sup 14/C-incorporation in the alcohol soluble and alcohol-ether soluble fractions was enhanced indicating that the insecticides considerably altered the glucose metabolism of the bacterium.}
place = {India}
year = {1980}
month = {Jan}
}