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Production of betalaines by Myrtillocactus cell cultures. Passage from heterotrophic state to autotrophic state with Asparagus cell cultures

Conference:

Abstract

Myrtillocactus tissue cultures are grown from the epicotyl of young plantlets. With an appropriate growing medium it is possible, after transfer of fragments of these cultures to a liquid environment, to obtain dissociation and proliferation of cells. The production of betalaic pigments is induced in solid surroundings by adjustement of the growing medium composition and can be maintained in a liquid environment. The multiplication of pigmented cells in suspension may thus be obtained. The conversion of Asparagus cell suspensions from the heterotrophic state (use of lactose as source of carbon) to the autotrophic state (carbon supplied by CO/sub 2/) is obtained by a gradual reduction in the sugar concentration of the medium combined with a rise in the CO/sub 2/ content of the gas mixture atmosphere injected into the cultivator. The passage to the autotrophic state of a Myrtillocactus suspension would enable the production conditions of a metabolite (Betalaine) to be studied by micro-algae culture techniques.
Publication Date:
Nov 01, 1982
Product Type:
Conference
Report Number:
CEA-CONF-6608; CONF-8211105-4
Reference Number:
FR-83-02593; ERA-08-047350; EDB-83-152508
Resource Relation:
Conference: Bombannes VALVA symposium, Bordeaux, France, 16 Nov 1982
Subject:
59 BASIC BIOLOGICAL SCIENCES; PIGMENTS; BIOSYNTHESIS; PLANTS; TISSUE CULTURES; CULTIVATION; CULTURE MEDIA; METABOLITES; SYNTHESIS; 550200* - Biochemistry
OSTI ID:
8294309
Research Organizations:
CEA Centre d'Etudes Nucleaires de Cadarache, 13 - Saint-Paul-les-Durance (France)
Country of Origin:
France
Language:
French
Other Identifying Numbers:
Other: ON: DE83751076
Availability:
NTIS (US Sales Only), PC A02/MF A01.
Submitting Site:
FR
Size:
Pages: 4
Announcement Date:
Mar 14, 2013

Conference:

Citation Formats

Bulard, C, Mary, J, Chaumont, D, and Gudin, C. Production of betalaines by Myrtillocactus cell cultures. Passage from heterotrophic state to autotrophic state with Asparagus cell cultures. France: N. p., 1982. Web.
Bulard, C, Mary, J, Chaumont, D, & Gudin, C. Production of betalaines by Myrtillocactus cell cultures. Passage from heterotrophic state to autotrophic state with Asparagus cell cultures. France.
Bulard, C, Mary, J, Chaumont, D, and Gudin, C. 1982. "Production of betalaines by Myrtillocactus cell cultures. Passage from heterotrophic state to autotrophic state with Asparagus cell cultures." France.
@misc{etde_8294309,
title = {Production of betalaines by Myrtillocactus cell cultures. Passage from heterotrophic state to autotrophic state with Asparagus cell cultures}
author = {Bulard, C, Mary, J, Chaumont, D, and Gudin, C}
abstractNote = {Myrtillocactus tissue cultures are grown from the epicotyl of young plantlets. With an appropriate growing medium it is possible, after transfer of fragments of these cultures to a liquid environment, to obtain dissociation and proliferation of cells. The production of betalaic pigments is induced in solid surroundings by adjustement of the growing medium composition and can be maintained in a liquid environment. The multiplication of pigmented cells in suspension may thus be obtained. The conversion of Asparagus cell suspensions from the heterotrophic state (use of lactose as source of carbon) to the autotrophic state (carbon supplied by CO/sub 2/) is obtained by a gradual reduction in the sugar concentration of the medium combined with a rise in the CO/sub 2/ content of the gas mixture atmosphere injected into the cultivator. The passage to the autotrophic state of a Myrtillocactus suspension would enable the production conditions of a metabolite (Betalaine) to be studied by micro-algae culture techniques.}
place = {France}
year = {1982}
month = {Nov}
}