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Bioconversion of cellulose to ethanol

Technical Report:

Abstract

Enzymatic hydrolysis of steam pretreated sallow gives highest yields of soluble sugars when hemicellulose is degraded already in the pretreatment step. The steam pretreatment equipment is rebuilt so that 75 g (dry matter) material instead of 7 g can be treated each time. The cellulose production has been increased 123% by the utilization of aqueous two-phase systems as compared to regular growth medium. The cellulase activity per gram of cellulose has been increased from 42 FPU in regular growth medium to 156 FPU in aqueous two-phase systems. Crude dextran can be used for enzyme production. Enzyme recovery up to 75% has been achieved by combining aqueous two-phase technique with membrane technique. Using the enzyme glucose isomerase in combination with S. cerevisiae theoretical yields in pentose fermentations have been achieved, with a product concentration of 60 g/L and a productivity of 2 g/L x h. Yeast and enzyme can be recirculated using membrane technique. Computer simulation shows that the rate equation for enzymatic hydrolysis with respect to inhibiting sugar concentrations can be used to interpolate with respect to sugar concentrations. Computer simulations show that hydrolysis experiments should focus on high substrate concentrations (>10%) using fed-batch technique and enzyme concentrations in the  More>>
Publication Date:
Jun 20, 1985
Product Type:
Technical Report
Report Number:
STEV-BF-85-12; LUTFD2/TFRT-5316-1-063
Reference Number:
NOR-85-07540; EDB-86-040172
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products. The project participates in the international collaboration IEA/FE project CPD-2, which is operated as a computer conferance. Includes two appendices. Appendix 1:1 see: Analytica Chimica Acta 163135-141, Evaluation of a dialysis probe for continuous sampling in fermentors and in complex media by C.F. Mandenius, B. Danielsson, B. Mattiasson. (Lund Univ. (Sweden). Dept. of Pure and Applied Biochemistry)
Subject:
09 BIOMASS FUELS; ETHANOL; BIOSYNTHESIS; HEMICELLULOSE; ENZYMATIC HYDROLYSIS; ADHESIVES; CELLULASE; CELLULOSE; COMPUTERIZED SIMULATION; DEXTRAN; FERMENTATION; LIGNIN; MEMBRANES; STEAM; WILLOWS; YEASTS; ALCOHOLS; BIOCONVERSION; BLOOD SUBSTITUTES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; DRUGS; ENZYMES; FUNGI; GLYCOSYL HYDROLASES; HEMATOLOGIC AGENTS; HYDROLASES; HYDROLYSIS; HYDROXY COMPOUNDS; LYSIS; MICROORGANISMS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SIMULATION; SOLVOLYSIS; SYNTHESIS; TREES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
7808270
Research Organizations:
Statens Energiverk, Stockholm (Sweden)
Country of Origin:
Sweden
Language:
Swedish and English
Other Identifying Numbers:
Other: ON: DE86750814
Availability:
NTIS (US Sales Only), PC A10/MF A01; 1.
Submitting Site:
NORD
Size:
Pages: 215
Announcement Date:
Mar 14, 2013

Technical Report:

Citation Formats

Hahn-Haegerdal, B, Mandenius, C F, Mattiasson, B, Nilsson, B, Axelsson, J P, and Hagander, P. Bioconversion of cellulose to ethanol. Sweden: N. p., 1985. Web.
Hahn-Haegerdal, B, Mandenius, C F, Mattiasson, B, Nilsson, B, Axelsson, J P, & Hagander, P. Bioconversion of cellulose to ethanol. Sweden.
Hahn-Haegerdal, B, Mandenius, C F, Mattiasson, B, Nilsson, B, Axelsson, J P, and Hagander, P. 1985. "Bioconversion of cellulose to ethanol." Sweden.
@misc{etde_7808270,
title = {Bioconversion of cellulose to ethanol}
author = {Hahn-Haegerdal, B, Mandenius, C F, Mattiasson, B, Nilsson, B, Axelsson, J P, and Hagander, P}
abstractNote = {Enzymatic hydrolysis of steam pretreated sallow gives highest yields of soluble sugars when hemicellulose is degraded already in the pretreatment step. The steam pretreatment equipment is rebuilt so that 75 g (dry matter) material instead of 7 g can be treated each time. The cellulose production has been increased 123% by the utilization of aqueous two-phase systems as compared to regular growth medium. The cellulase activity per gram of cellulose has been increased from 42 FPU in regular growth medium to 156 FPU in aqueous two-phase systems. Crude dextran can be used for enzyme production. Enzyme recovery up to 75% has been achieved by combining aqueous two-phase technique with membrane technique. Using the enzyme glucose isomerase in combination with S. cerevisiae theoretical yields in pentose fermentations have been achieved, with a product concentration of 60 g/L and a productivity of 2 g/L x h. Yeast and enzyme can be recirculated using membrane technique. Computer simulation shows that the rate equation for enzymatic hydrolysis with respect to inhibiting sugar concentrations can be used to interpolate with respect to sugar concentrations. Computer simulations show that hydrolysis experiments should focus on high substrate concentrations (>10%) using fed-batch technique and enzyme concentrations in the range of 2-8% in relation to substrate dry matter. The combined 'flow injection analysis', FIA, and enzyme reactor probe has been adapted to enzymatic saccarifications of sodium hydroxide pretreated sallow. The gas membrane sensor for ethanol has been utilized in simultaneous saccharification and fermentation of sodium hydroxide pretreated sallow. A literature study concerning pervaporation for ethanol up-grading has been made.(Author).}
place = {Sweden}
year = {1985}
month = {Jun}
}