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Development of a method for testing the susceptibility of Salix alba to Erwinia salicis

Technical Report:

Abstract

Salix is one of the faster growing tree species that has potential for biomass plantations in Europe. This potential, however, is limited by the occurrence of a vascular disease caused by the bacterium Erwinia salicis (Day) Chester. Selection and breeding of disease resistance is hampered by insufficient knowledge of the infection biology and ecology of the pathogen. The availability of specific antisera is of vital importance in the study of the infection biology and in monitoring bacterial populations inside the host. Therefore, one of the aims of the research was to improve the antisera. The composition of the soluble antigens of E. salicis was studied by enzyme-linked immunosorbent assay (ELISA), polyacrylamide gel electrophoresis (PAGE) and electro-immunoblotting. Antiserum prepared to whole cells of E. salicis mainly contains antibodies to bacterial surface antigens or to soluble antigens. ELISA demonstrated the presence of bacterial soluble antigens in leaves of diseased willow trees and also in leaves of willow shoots which were placed in a solution of bacterial soluble antigens. An inoculation experiment was carried out to ascertain if there is a statistically significant correlation between plant water potential and response to artificial inoculation with E. salicis. To elucidate the mechanisms involved in pathogenicity  More>>
Authors:
Dingjan-Versteegh, A M; de Kam, M [1] 
  1. Institute for Forestry and Urban Ecology, Wageningen (Netherlands)
Publication Date:
Jan 01, 1990
Product Type:
Technical Report
Report Number:
EUR-12633
Reference Number:
EDB-91-029596; ERA-91-011144
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; BACTERIAL DISEASES; IMMUNE SERUMS; WILLOWS; DISEASE RESISTANCE; ANTIBODY FORMATION; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BACTERIA; BIOLOGICAL STRESS; BIOMASS PLANTATIONS; DIAGNOSIS; DISEASE INCIDENCE; DNA-CLONING; ELECTROPHORESIS; ENZYME IMMUNOASSAY; IMMUNOASSAY; INOCULATION; PATHOGENESIS; PLANT BREEDING; PLANT DISEASES; PROGRESS REPORT; VIRULENCE; BIOASSAY; CLONING; DISEASES; DNA HYBRIDIZATION; DOCUMENT TYPES; HYBRIDIZATION; INFECTIOUS DISEASES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MICROORGANISMS; PLANTS; TREES; 553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-); 550700 - Microbiology; 090700 - Biomass Fuels- Resources- (1990-)
Sponsoring Organizations:
CEC
OSTI ID:
7794604
Research Organizations:
Commission of the European Communities, Luxembourg (Luxembourg). Directorate General Telecommunications, Information Industries and Innovation; Institute for Forestry and Urban Ecology, Wageningen (Netherlands)
Country of Origin:
Luxembourg
Language:
English
Other Identifying Numbers:
Other: ON: TI91006996; CNN: EN3B-0032-NL
Availability:
OSTI; NTIS (US Sales Only); Office for Official Publications of the European Communities, 2, rue Mercier, L-2985 Luxembourg
Submitting Site:
TIC
Size:
Pages: 45 p
Announcement Date:
Mar 14, 2013

Technical Report:

Citation Formats

Dingjan-Versteegh, A M, and de Kam, M. Development of a method for testing the susceptibility of Salix alba to Erwinia salicis. Luxembourg: N. p., 1990. Web.
Dingjan-Versteegh, A M, & de Kam, M. Development of a method for testing the susceptibility of Salix alba to Erwinia salicis. Luxembourg.
Dingjan-Versteegh, A M, and de Kam, M. 1990. "Development of a method for testing the susceptibility of Salix alba to Erwinia salicis." Luxembourg.
@misc{etde_7794604,
title = {Development of a method for testing the susceptibility of Salix alba to Erwinia salicis}
author = {Dingjan-Versteegh, A M, and de Kam, M}
abstractNote = {Salix is one of the faster growing tree species that has potential for biomass plantations in Europe. This potential, however, is limited by the occurrence of a vascular disease caused by the bacterium Erwinia salicis (Day) Chester. Selection and breeding of disease resistance is hampered by insufficient knowledge of the infection biology and ecology of the pathogen. The availability of specific antisera is of vital importance in the study of the infection biology and in monitoring bacterial populations inside the host. Therefore, one of the aims of the research was to improve the antisera. The composition of the soluble antigens of E. salicis was studied by enzyme-linked immunosorbent assay (ELISA), polyacrylamide gel electrophoresis (PAGE) and electro-immunoblotting. Antiserum prepared to whole cells of E. salicis mainly contains antibodies to bacterial surface antigens or to soluble antigens. ELISA demonstrated the presence of bacterial soluble antigens in leaves of diseased willow trees and also in leaves of willow shoots which were placed in a solution of bacterial soluble antigens. An inoculation experiment was carried out to ascertain if there is a statistically significant correlation between plant water potential and response to artificial inoculation with E. salicis. To elucidate the mechanisms involved in pathogenicity and virulence of E. salicis, attempts were made to isolate the plasmids of the bacterium, however, it appears that E. salicis does not have plasmids. In collaboration with Ch. Maceau (21), the E. salicis genome was isolated and a genomic library was constructed in E. coli. 28 refs., 10 figs., 4 tabs.}
place = {Luxembourg}
year = {1990}
month = {Jan}
}