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Ethanol production from cellulose, lactose and xylose using yeasts and enzymes. Gewinnung von Ethanol aus Cellulose, Lactose, und Xylose mit Hilfe von Hefen und Enzymen

Abstract

Experiments with mixtures of whey and corn showed that more than 85% of the lactose was degraded into ethanol. The applicability of cellulose was investigated by means of potatoes. Cellulase is inhibited by glucose, which is a fermentation intermediate, as well as by the end product ethanol. A cellulase inhibitor in potatoes was detected and stabilized; this inhibitor could be degraded into neutral components by a suitable enzyme. Saccharification and fermentation experiments showed that the cellulose fraction of potatoes can be reduced efficiently. The effects of non-enzymatic pretreatment on enzymatic degradation of cellulose, combined with fermentation of the degradation products, are illustrated by the example of cellulose treated with acid and alkaline substances. A continuous fermentation system was developed from which the ethanol is withdrawn in vapour form. The system made better use of the cellulase activity and increased the efficiency of a xylose-fermenting yeast. The new method is compared with batch experiments in order to assess its efficiency. The advantages of the continuous process are proved for two yeasts of the species Pachysolu and Pichia. Specific fermentation rates up to 0.08 g/(g x h) and fermentation yields up to 0.42 g ethanol/g xylose were achieved with Pichia stipitis.
Authors:
Publication Date:
Jul 03, 1986
Product Type:
Technical Report
Report Number:
NP-9770002
Reference Number:
DE-88-011091; ERA-13-053392; EDB-88-167321
Resource Relation:
Other Information: Diss. (Dr.rer.nat.). Portions of this document are illegible in microfiche products
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CELLULOSE; FERMENTATION; ETHANOL; BIOSYNTHESIS; LACTOSE; XYLOSE; ALKALINE HYDROLYSIS; BIODEGRADATION; CELLULASE; ENZYMATIC HYDROLYSIS; ENZYME ACTIVITY; ENZYME INHIBITORS; GLUCOSE; POTATOES; SACCHAROSE; WHEY; YEASTS; ALCOHOLS; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; DISACCHARIDES; ENZYMES; FOOD; FUNGI; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; HYDROXY COMPOUNDS; INDUSTRIAL WASTES; LYSIS; MICROORGANISMS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; SYNTHESIS; TUBERS; VEGETABLES; WASTES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550200 - Biochemistry
OSTI ID:
7777425
Research Organizations:
Technische Univ. Muenchen, Freising (Germany, F.R.). Fakultaet Brauwesen, Lebensmitteltechnologie und Milchwissenschaft
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Other: ON: DE89770002
Availability:
NTIS (US Sales Only), PC A09/MF A01; 1.
Submitting Site:
DE
Size:
Pages: 190
Announcement Date:
Sep 01, 1988

Citation Formats

Schwank, U. Ethanol production from cellulose, lactose and xylose using yeasts and enzymes. Gewinnung von Ethanol aus Cellulose, Lactose, und Xylose mit Hilfe von Hefen und Enzymen. Germany: N. p., 1986. Web.
Schwank, U. Ethanol production from cellulose, lactose and xylose using yeasts and enzymes. Gewinnung von Ethanol aus Cellulose, Lactose, und Xylose mit Hilfe von Hefen und Enzymen. Germany.
Schwank, U. 1986. "Ethanol production from cellulose, lactose and xylose using yeasts and enzymes. Gewinnung von Ethanol aus Cellulose, Lactose, und Xylose mit Hilfe von Hefen und Enzymen." Germany.
@misc{etde_7777425,
title = {Ethanol production from cellulose, lactose and xylose using yeasts and enzymes. Gewinnung von Ethanol aus Cellulose, Lactose, und Xylose mit Hilfe von Hefen und Enzymen}
author = {Schwank, U}
abstractNote = {Experiments with mixtures of whey and corn showed that more than 85% of the lactose was degraded into ethanol. The applicability of cellulose was investigated by means of potatoes. Cellulase is inhibited by glucose, which is a fermentation intermediate, as well as by the end product ethanol. A cellulase inhibitor in potatoes was detected and stabilized; this inhibitor could be degraded into neutral components by a suitable enzyme. Saccharification and fermentation experiments showed that the cellulose fraction of potatoes can be reduced efficiently. The effects of non-enzymatic pretreatment on enzymatic degradation of cellulose, combined with fermentation of the degradation products, are illustrated by the example of cellulose treated with acid and alkaline substances. A continuous fermentation system was developed from which the ethanol is withdrawn in vapour form. The system made better use of the cellulase activity and increased the efficiency of a xylose-fermenting yeast. The new method is compared with batch experiments in order to assess its efficiency. The advantages of the continuous process are proved for two yeasts of the species Pachysolu and Pichia. Specific fermentation rates up to 0.08 g/(g x h) and fermentation yields up to 0.42 g ethanol/g xylose were achieved with Pichia stipitis.}
place = {Germany}
year = {1986}
month = {Jul}
}