Abstract
Radioreceptor assay of insulin was discussed from the aspects of the measuring method, its merits and problems to be solved, and its clinical application. Rat liver 10 x g pellet was used as receptor site, and enzymatic degradation of insulin by the system contained in this fraction was inhibited by adding 1 mM p-CMB. /sup 125/I-labelled porcine insulin was made by lactoperoxidase method under overnight incubation at 4/sup 0/C and later purification by Sephadex G-25 column and Whatman CF-11 cellulose powder. Dog pancreatic vein serum insulin during and after the glucose load was determined by radioreceptor assay and radioimmunoassay resulting that both measurements accorded considerably. Radioreceptor assay would clarify the pathology of disorders of glucose metabolism including diabetes.
Suzuki, Kazuo
[1]
- Tokyo Univ. (Japan). Faculty of Medicine
Citation Formats
Suzuki, Kazuo.
Radioreceptor assay for insulin.
Japan: N. p.,
1975.
Web.
Suzuki, Kazuo.
Radioreceptor assay for insulin.
Japan.
Suzuki, Kazuo.
1975.
"Radioreceptor assay for insulin."
Japan.
@misc{etde_7365200,
title = {Radioreceptor assay for insulin}
author = {Suzuki, Kazuo}
abstractNote = {Radioreceptor assay of insulin was discussed from the aspects of the measuring method, its merits and problems to be solved, and its clinical application. Rat liver 10 x g pellet was used as receptor site, and enzymatic degradation of insulin by the system contained in this fraction was inhibited by adding 1 mM p-CMB. /sup 125/I-labelled porcine insulin was made by lactoperoxidase method under overnight incubation at 4/sup 0/C and later purification by Sephadex G-25 column and Whatman CF-11 cellulose powder. Dog pancreatic vein serum insulin during and after the glucose load was determined by radioreceptor assay and radioimmunoassay resulting that both measurements accorded considerably. Radioreceptor assay would clarify the pathology of disorders of glucose metabolism including diabetes.}
journal = []
volume = {30:4}
journal type = {AC}
place = {Japan}
year = {1975}
month = {Apr}
}
title = {Radioreceptor assay for insulin}
author = {Suzuki, Kazuo}
abstractNote = {Radioreceptor assay of insulin was discussed from the aspects of the measuring method, its merits and problems to be solved, and its clinical application. Rat liver 10 x g pellet was used as receptor site, and enzymatic degradation of insulin by the system contained in this fraction was inhibited by adding 1 mM p-CMB. /sup 125/I-labelled porcine insulin was made by lactoperoxidase method under overnight incubation at 4/sup 0/C and later purification by Sephadex G-25 column and Whatman CF-11 cellulose powder. Dog pancreatic vein serum insulin during and after the glucose load was determined by radioreceptor assay and radioimmunoassay resulting that both measurements accorded considerably. Radioreceptor assay would clarify the pathology of disorders of glucose metabolism including diabetes.}
journal = []
volume = {30:4}
journal type = {AC}
place = {Japan}
year = {1975}
month = {Apr}
}