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Radioreceptor assay for insulin

Journal Article:

Abstract

Radioreceptor assay of insulin was discussed from the aspects of the measuring method, its merits and problems to be solved, and its clinical application. Rat liver 10 x g pellet was used as receptor site, and enzymatic degradation of insulin by the system contained in this fraction was inhibited by adding 1 mM p-CMB. /sup 125/I-labelled porcine insulin was made by lactoperoxidase method under overnight incubation at 4/sup 0/C and later purification by Sephadex G-25 column and Whatman CF-11 cellulose powder. Dog pancreatic vein serum insulin during and after the glucose load was determined by radioreceptor assay and radioimmunoassay resulting that both measurements accorded considerably. Radioreceptor assay would clarify the pathology of disorders of glucose metabolism including diabetes.
Authors:
Suzuki, Kazuo [1] 
  1. Tokyo Univ. (Japan). Faculty of Medicine
Publication Date:
Apr 01, 1975
Product Type:
Journal Article
Reference Number:
EDB-76-043725
Resource Relation:
Journal Name: Saishin Igaku; (Japan); Journal Volume: 30:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; INSULIN; RADIOASSAY; CHEMORECEPTORS; DIABETES MELLITUS; DOGS; IODINE 125; LABELLED COMPOUNDS; LIVER; RATS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE DISEASES; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HORMONES; RADIOISOTOPES; RODENTS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7365200
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: SAIGA
Submitting Site:
INIS
Size:
Pages: 601-606
Announcement Date:

Journal Article:

Citation Formats

Suzuki, Kazuo. Radioreceptor assay for insulin. Japan: N. p., 1975. Web.
Suzuki, Kazuo. Radioreceptor assay for insulin. Japan.
Suzuki, Kazuo. 1975. "Radioreceptor assay for insulin." Japan.
@misc{etde_7365200,
title = {Radioreceptor assay for insulin}
author = {Suzuki, Kazuo}
abstractNote = {Radioreceptor assay of insulin was discussed from the aspects of the measuring method, its merits and problems to be solved, and its clinical application. Rat liver 10 x g pellet was used as receptor site, and enzymatic degradation of insulin by the system contained in this fraction was inhibited by adding 1 mM p-CMB. /sup 125/I-labelled porcine insulin was made by lactoperoxidase method under overnight incubation at 4/sup 0/C and later purification by Sephadex G-25 column and Whatman CF-11 cellulose powder. Dog pancreatic vein serum insulin during and after the glucose load was determined by radioreceptor assay and radioimmunoassay resulting that both measurements accorded considerably. Radioreceptor assay would clarify the pathology of disorders of glucose metabolism including diabetes.}
journal = {Saishin Igaku; (Japan)}
volume = {30:4}
journal type = {AC}
place = {Japan}
year = {1975}
month = {Apr}
}