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Purification and some properties of G-factor from the silk gland of silkworm

Journal Article:

Abstract

Purification of G-factor from the silk gland of silkworms and some of its properties have been studied in comparison with the G-factor from E. coli and rat liver transferase II. In the experiment, GTP-..gamma..-/sup 32/P was prepared from GDP and /sup 32/Pi by photophosphorylation with spinach chloroplasts, and purified by Dowex-1 chromatography. The purified G-factor was homogeneous on disc gel electrophoresis. Molecular weight of 80,000 was estimated from a calibrated Sephadex G-200 column. The purified G-factor exhibited uncoupled ribosome-dependent GTPase activity, and was inhibited by fusidic acid, SH inhibitor, and diphtheria toxin plus NAD. The G-factor from E. coli and that from the silk gland were not interchangeable in GTPase reaction.
Authors:
Taira, H; Ejiri, S I; Shimura, K [1] 
  1. Tohoku Univ., Sendai (Japan). Faculty of Agriculture
Publication Date:
Dec 01, 1972
Product Type:
Journal Article
Reference Number:
AIX-07-244498; EDB-76-076663
Resource Relation:
Journal Name: J. Biochem. (Tokyo); (Japan); Journal Volume: 72:6
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLANDS; BIOCHEMISTRY; BIOSYNTHESIS; CATALYSIS; METABOLISM; PHOSPHATASES; PHOSPHORUS 32; PROTEINS; RIBOSOMES; SILKWORM; TRACER TECHNIQUES; ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CELL CONSTITUENTS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; ENZYMES; ESTERASES; HYDROLASES; INSECTS; INVERTEBRATES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
7358412
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JOBIA
Submitting Site:
INIS
Size:
Pages: 1527-1535
Announcement Date:

Journal Article:

Citation Formats

Taira, H, Ejiri, S I, and Shimura, K. Purification and some properties of G-factor from the silk gland of silkworm. Japan: N. p., 1972. Web.
Taira, H, Ejiri, S I, & Shimura, K. Purification and some properties of G-factor from the silk gland of silkworm. Japan.
Taira, H, Ejiri, S I, and Shimura, K. 1972. "Purification and some properties of G-factor from the silk gland of silkworm." Japan.
@misc{etde_7358412,
title = {Purification and some properties of G-factor from the silk gland of silkworm}
author = {Taira, H, Ejiri, S I, and Shimura, K}
abstractNote = {Purification of G-factor from the silk gland of silkworms and some of its properties have been studied in comparison with the G-factor from E. coli and rat liver transferase II. In the experiment, GTP-..gamma..-/sup 32/P was prepared from GDP and /sup 32/Pi by photophosphorylation with spinach chloroplasts, and purified by Dowex-1 chromatography. The purified G-factor was homogeneous on disc gel electrophoresis. Molecular weight of 80,000 was estimated from a calibrated Sephadex G-200 column. The purified G-factor exhibited uncoupled ribosome-dependent GTPase activity, and was inhibited by fusidic acid, SH inhibitor, and diphtheria toxin plus NAD. The G-factor from E. coli and that from the silk gland were not interchangeable in GTPase reaction.}
journal = {J. Biochem. (Tokyo); (Japan)}
volume = {72:6}
journal type = {AC}
place = {Japan}
year = {1972}
month = {Dec}
}