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Studies on radioimmunoassay of peptide hormone using polyethyleneglycol. I. Insulin

Journal Article:

Abstract

Radioimmunoassay (RIA) of insulin using polyethyleneglycol (PEG) was examined for measurement conditions such as the concentration, reaction time, temperature, and amount of serum to be added in order to allow uniform separations of free insulin and bound insulin. The standard curve of the present method was in good agreement with that of the two antibody method, and the two methods showed a highly significant correlation (r=0.98, p<0.001). The reproducibility showed only the fluctuations ranging from 0.9 to 4.9%, and the recovery rate was between 70 and 100%. Since the insulin RIA by PEG is convenient and economical and yields more stable results than those obtained by the two antibody method, it is possible to use it for RIA of other peptide hormones such as glucagon.
Authors:
Kihara, A; Kikuchi, A; Yaegashi, T; Ohhara, H [1] 
  1. Sapporo Medical Coll. (Japan)
Publication Date:
Jun 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-260361; EDB-77-014347
Resource Relation:
Journal Name: Rinsho Byori; (Japan); Journal Volume: 23:6
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; INSULIN; RADIOIMMUNOASSAY; CALIBRATION STANDARDS; GLOBULINS-GAMMA; IODINE 125; LABELLED COMPOUNDS; PH VALUE; POLYETHYLENE GLYCOLS; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PEPTIDE HORMONES; POLYMERS; PROTEINS; RADIOASSAY; RADIOISOTOPES; STANDARDS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7345055
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: RBYOA
Submitting Site:
INIS
Size:
Pages: 449-452
Announcement Date:
Oct 01, 1976

Journal Article:

Citation Formats

Kihara, A, Kikuchi, A, Yaegashi, T, and Ohhara, H. Studies on radioimmunoassay of peptide hormone using polyethyleneglycol. I. Insulin. Japan: N. p., 1975. Web.
Kihara, A, Kikuchi, A, Yaegashi, T, & Ohhara, H. Studies on radioimmunoassay of peptide hormone using polyethyleneglycol. I. Insulin. Japan.
Kihara, A, Kikuchi, A, Yaegashi, T, and Ohhara, H. 1975. "Studies on radioimmunoassay of peptide hormone using polyethyleneglycol. I. Insulin." Japan.
@misc{etde_7345055,
title = {Studies on radioimmunoassay of peptide hormone using polyethyleneglycol. I. Insulin}
author = {Kihara, A, Kikuchi, A, Yaegashi, T, and Ohhara, H}
abstractNote = {Radioimmunoassay (RIA) of insulin using polyethyleneglycol (PEG) was examined for measurement conditions such as the concentration, reaction time, temperature, and amount of serum to be added in order to allow uniform separations of free insulin and bound insulin. The standard curve of the present method was in good agreement with that of the two antibody method, and the two methods showed a highly significant correlation (r=0.98, p<0.001). The reproducibility showed only the fluctuations ranging from 0.9 to 4.9%, and the recovery rate was between 70 and 100%. Since the insulin RIA by PEG is convenient and economical and yields more stable results than those obtained by the two antibody method, it is possible to use it for RIA of other peptide hormones such as glucagon.}
journal = {Rinsho Byori; (Japan)}
volume = {23:6}
journal type = {AC}
place = {Japan}
year = {1975}
month = {Jun}
}