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Resistance of Micrococcus radiodurans to killing and mutation by agents which damage DNA

Abstract

The resistance of Micrococcus radiodurans to the lethal and mutagenic action of ultraviolet (UV) light, ionising (..gamma..) radiation, mitomycin C (MTC), nitrous acid (NA), hydroxylamine (HA), N-methyl-N'-nitro-N-nitrosoguanidine (NG), ethylmethanesulphonate (EMS) and ..beta..-propiolactone (..beta..PL) has been compared with that of Escherichia coli B/r. M. radiodurans was much more resistant than E. coli B/r to the lethal effects of UV light (by a factor of 33), ..gamma..-radiation (55), NG (15) and NA (62), showed intermediate resistance to MTC (4) and HA (7), but was sensitive to EMS (1) and ..beta..PL (2). M. radiodurans was very resistant to mutagens producing damage which can be repaired by a recombination system, indicating that it possesses an extremely efficient recombination repair mechanism. Both species were equally sensitive to mutation to trimethoprim resistance by NG, but M. radiodurans was more resistant than E. coli B/r to the other mutagens tested, being non-mutable by UV light, ..gamma..-radiation, MTC and HA, and only slightly sensitive to mutation by NA, EMS, and ..beta..PL. The resistance of M. radiodurans to mutation by UV light, ..gamma.. radiation and MTC is consistent with an hypothesis that recombination repair in M. radiodurans is accurate since these mutagens may depend on an 'error-prone' recombination system  More>>
Authors:
Sweet, D M; Moseley, B E.B. [1] 
  1. Edinburgh Univ. (UK). School of Agriculture
Publication Date:
Feb 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-07-260068; EDB-77-014534
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 34:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; MICROCOCCUS RADIODURANS; RADIOSENSITIVITY; CELL KILLING; COMPARATIVE EVALUATIONS; EFFICIENCY; EMS; ESCHERICHIA COLI; GAMMA RADIATION; GENE RECOMBINATION; GENETIC EFFECTS; GENETIC RADIATION EFFECTS; HYDROXYLAMINE; LACTONES; MITOMYCIN; MUTAGENESIS; MUTAGENS; NITROSO COMPOUNDS; NITROUS ACID; SENSITIVITY; ULTRAVIOLET RADIATION; AMINES; ANTIBIOTICS; ANTIMITOTIC DRUGS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; ESTERS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; INORGANIC ACIDS; IONIZING RADIATIONS; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SULFONIC ACID ESTERS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7344982
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
INIS
Size:
Pages: 175-186
Announcement Date:
Oct 01, 1976

Citation Formats

Sweet, D M, and Moseley, B E.B. Resistance of Micrococcus radiodurans to killing and mutation by agents which damage DNA. Netherlands: N. p., 1976. Web.
Sweet, D M, & Moseley, B E.B. Resistance of Micrococcus radiodurans to killing and mutation by agents which damage DNA. Netherlands.
Sweet, D M, and Moseley, B E.B. 1976. "Resistance of Micrococcus radiodurans to killing and mutation by agents which damage DNA." Netherlands.
@misc{etde_7344982,
title = {Resistance of Micrococcus radiodurans to killing and mutation by agents which damage DNA}
author = {Sweet, D M, and Moseley, B E.B.}
abstractNote = {The resistance of Micrococcus radiodurans to the lethal and mutagenic action of ultraviolet (UV) light, ionising (..gamma..) radiation, mitomycin C (MTC), nitrous acid (NA), hydroxylamine (HA), N-methyl-N'-nitro-N-nitrosoguanidine (NG), ethylmethanesulphonate (EMS) and ..beta..-propiolactone (..beta..PL) has been compared with that of Escherichia coli B/r. M. radiodurans was much more resistant than E. coli B/r to the lethal effects of UV light (by a factor of 33), ..gamma..-radiation (55), NG (15) and NA (62), showed intermediate resistance to MTC (4) and HA (7), but was sensitive to EMS (1) and ..beta..PL (2). M. radiodurans was very resistant to mutagens producing damage which can be repaired by a recombination system, indicating that it possesses an extremely efficient recombination repair mechanism. Both species were equally sensitive to mutation to trimethoprim resistance by NG, but M. radiodurans was more resistant than E. coli B/r to the other mutagens tested, being non-mutable by UV light, ..gamma..-radiation, MTC and HA, and only slightly sensitive to mutation by NA, EMS, and ..beta..PL. The resistance of M. radiodurans to mutation by UV light, ..gamma.. radiation and MTC is consistent with an hypothesis that recombination repair in M. radiodurans is accurate since these mutagens may depend on an 'error-prone' recombination system for their mutagenic effect in E. coli B/r. However, because M. radiodurans is also resistant to mutagens such as HA and EMS, which are mutagenic in E. coli in the absence of an 'error-prone' system, we propose that all the mutagens tested may have a common mode of action in E. coli B/r, but that this mutagenic pathway is missing in M. radiodurans.}
journal = []
volume = {34:2}
journal type = {AC}
place = {Netherlands}
year = {1976}
month = {Feb}
}