You need JavaScript to view this

Proteolytic activity of beef liver determined by natural /sup 125/I-labelled substrates

Journal Article:

Abstract

The method of determining the enzymatic activity of acid proteinases is described. The method is based on the use of /sup 125/I-labelled natural protein substrates. /sup 125/I-labelled albumin, /sup 125/I-globulin and /sup 125/I-insulin were tested for the determination of activities. All the substrates were hydrolyzed with the enzymes of the supernatant fraction (106,000 g) of beef liver homogenate in the acid pH zone. Optimum enzymatic reaction conditions were tested, the dependence of the reaction on the enzyme concentration, time and temperature was determined, pH optimum was ascertained for the individual substrates, and pH stability was determined. The results show that the method is suitable for determining the enzymatic activity of proteinases of cathepsin character.
Authors:
Blahovec, J; Ondrus, I [1] 
  1. Vysoka Skola Veterinarska, Kosice (Czechoslovakia)
Publication Date:
Jul 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-08-283724; EDB-77-038585
Resource Relation:
Journal Name: Vet. Med. (Prague); (Czechoslovakia); Journal Volume: 20:7
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEOLYSIS; TRACER TECHNIQUES; ALBUMINS; CATALYSIS; CATHEPSINS; CATTLE; HYDROLYSIS; IMMUNOGLOBULINS; INSULIN; IODINE 125; LABELLED COMPOUNDS; LIVER; METABOLISM; PH VALUE; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; DECOMPOSITION; DIGESTIVE SYSTEM; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; GLANDS; GLOBULINS; HORMONES; HYDROLASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LYSIS; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PEPTIDE HYDROLASES; PROTEINS; RADIOISOTOPES; RUMINANTS; SOLVOLYSIS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
7336216
Country of Origin:
Serbia and Montenegro
Language:
Slovak
Other Identifying Numbers:
Journal ID: CODEN: VTMDA
Submitting Site:
INIS
Size:
Pages: 399-406
Announcement Date:

Journal Article:

Citation Formats

Blahovec, J, and Ondrus, I. Proteolytic activity of beef liver determined by natural /sup 125/I-labelled substrates. Serbia and Montenegro: N. p., 1975. Web.
Blahovec, J, & Ondrus, I. Proteolytic activity of beef liver determined by natural /sup 125/I-labelled substrates. Serbia and Montenegro.
Blahovec, J, and Ondrus, I. 1975. "Proteolytic activity of beef liver determined by natural /sup 125/I-labelled substrates." Serbia and Montenegro.
@misc{etde_7336216,
title = {Proteolytic activity of beef liver determined by natural /sup 125/I-labelled substrates}
author = {Blahovec, J, and Ondrus, I}
abstractNote = {The method of determining the enzymatic activity of acid proteinases is described. The method is based on the use of /sup 125/I-labelled natural protein substrates. /sup 125/I-labelled albumin, /sup 125/I-globulin and /sup 125/I-insulin were tested for the determination of activities. All the substrates were hydrolyzed with the enzymes of the supernatant fraction (106,000 g) of beef liver homogenate in the acid pH zone. Optimum enzymatic reaction conditions were tested, the dependence of the reaction on the enzyme concentration, time and temperature was determined, pH optimum was ascertained for the individual substrates, and pH stability was determined. The results show that the method is suitable for determining the enzymatic activity of proteinases of cathepsin character.}
journal = {Vet. Med. (Prague); (Czechoslovakia)}
volume = {20:7}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1975}
month = {Jul}
}