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Cell lysis and superoxide dismutase activities of highly radioresistant bacteria

Abstract

The highly radioresistant bacterium, Arthrobacter radiotolerans, has been isolated from the radioactive hot spring of Misasa, and it does not sporulate, it is Gram-positive, and its color is pink to red. This bacterium shows the highest resistance to gamma-ray among Gram-positive resistants, but the lytic enzyme capable of lysing the cells of strong radioresistants and the surface structure of the cells are little known except those about Micrococcus radiodurans. The cells of the M. radiodurans can be lysed by Achramobacter lyticus enzyme, and electron microscopic observation and chemical analysis revealed the mutilayered surface structure of the cells consisting of an inner membrane, a mucopeptide wall layer and a very outer layer. The superoxide dismutase (SOD) activity of aerobic and anaerobic bacteria was studied, and the relatively high SOD activity of the M. radiodurans was found. The SOD function acts against the threat posed by the reactive superoxide radical being generated biologically, photochemically and radiochemically in the presence of molecular oxygen. In this paper, it is reported that the lytic enzyme No.2 obtained from Cytophaga sp., containing N-acetyl-muramyl-L-alanine amidase, peptidase and endopeptidase, and showing broad lytic spectra, was able to lyse the cells of A. radiotolerans and four radioresistant micrococci, and  More>>
Authors:
Yoshinaka, T; Yano, K; Yamaguchi, H [1] 
  1. Tokyo Univ. (Japan). Faculty of Agriculture
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-283592; EDB-77-038671
Resource Relation:
Journal Name: Agric. Biol. Chem. (Tokyo); (Japan); Journal Volume: 40:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MICROCOCCUS RADIODURANS; OXIDOREDUCTASES; BIOCHEMISTRY; CATALYSIS; DECOMPOSITION; ESCHERICHIA COLI; METABOLISM; PEROXIDASES; TOXINS; ANTIGENS; BACTERIA; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CHEMISTRY; ENZYMES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; MICROCOCCUS; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PORPHYRINS; TOXIC MATERIALS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7336198
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ABCHA
Submitting Site:
INIS
Size:
Pages: 227-229
Announcement Date:
Feb 01, 1977

Citation Formats

Yoshinaka, T, Yano, K, and Yamaguchi, H. Cell lysis and superoxide dismutase activities of highly radioresistant bacteria. Japan: N. p., 1976. Web.
Yoshinaka, T, Yano, K, & Yamaguchi, H. Cell lysis and superoxide dismutase activities of highly radioresistant bacteria. Japan.
Yoshinaka, T, Yano, K, and Yamaguchi, H. 1976. "Cell lysis and superoxide dismutase activities of highly radioresistant bacteria." Japan.
@misc{etde_7336198,
title = {Cell lysis and superoxide dismutase activities of highly radioresistant bacteria}
author = {Yoshinaka, T, Yano, K, and Yamaguchi, H}
abstractNote = {The highly radioresistant bacterium, Arthrobacter radiotolerans, has been isolated from the radioactive hot spring of Misasa, and it does not sporulate, it is Gram-positive, and its color is pink to red. This bacterium shows the highest resistance to gamma-ray among Gram-positive resistants, but the lytic enzyme capable of lysing the cells of strong radioresistants and the surface structure of the cells are little known except those about Micrococcus radiodurans. The cells of the M. radiodurans can be lysed by Achramobacter lyticus enzyme, and electron microscopic observation and chemical analysis revealed the mutilayered surface structure of the cells consisting of an inner membrane, a mucopeptide wall layer and a very outer layer. The superoxide dismutase (SOD) activity of aerobic and anaerobic bacteria was studied, and the relatively high SOD activity of the M. radiodurans was found. The SOD function acts against the threat posed by the reactive superoxide radical being generated biologically, photochemically and radiochemically in the presence of molecular oxygen. In this paper, it is reported that the lytic enzyme No.2 obtained from Cytophaga sp., containing N-acetyl-muramyl-L-alanine amidase, peptidase and endopeptidase, and showing broad lytic spectra, was able to lyse the cells of A. radiotolerans and four radioresistant micrococci, and the radioresistant bacteria showedrelatively high SOD activity except M. sp. 248. It is well known that superoxide anions are generated by aerobic irradiation, and are toxic to microbial cells.}
journal = []
volume = {40:1}
journal type = {AC}
place = {Japan}
year = {1976}
month = {Jan}
}