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Interactions between the metabolism of L-leucine and D-glucose in the pancreatic. beta. -cells

Journal Article:

Abstract

..beta..-cell-rich pancreatic islets microdissected from obese-hyperglycemic mice were used to study interactions between the metabolism of L-leucine and D-glucose. L-leucine reduced the islet content of aspartic acid whereas D-glucose, when added to L-leucine-incubated islets, increased the contents of aspartic acid and ..gamma..-aminobutyric acid (GABA). D-glucose also increased the incorporation of L-leucine carbon into aspartic acid, GABA and glutamic acid, suggesting stimulation of a malate shuttle mechanism. When expressed per mole of the individual amino acids, the incorporation of L-leucine carbon into GABA was 2.5 - 4 times higher than into glutamic acid indicating intracellular compartmentation of the latter amino acid. Both L-leucine and D-leucine stimulated /sup 14/CO/sub 2/ production from /sup 14/C-labelled D-glucose. L-leucine did not affect /sup 3/H/sub 2/O production from tritiated D-glucose. The present data do not indicate a role of other amino acids or D-glucose in L-leucine-stimulated insulin release.
Authors:
Gylfe, E; Sehlin, J [1] 
  1. Umeaa Univ. (Sweden). Dept. of Histology
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-07-257512; EDB-77-008676
Resource Relation:
Journal Name: Horm. Metab. Res.; (Germany, Federal Republic of); Journal Volume: 8:1; Other Information: 1 fig.; 3 tabs.; 21 refs
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOSE; METABOLISM; LEUCINE; CARBON 14 COMPOUNDS; COMPARTMENTS; INSULIN; MICE; OXIDATION; PANCREAS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALDEHYDES; AMINO ACIDS; ANIMALS; BODY; CARBOHYDRATES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; DIGESTIVE SYSTEM; ENDOCRINE GLANDS; GLANDS; HEXOSES; HORMONES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MONOSACCHARIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; RODENTS; SACCHARIDES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
7333960
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: HMMRA
Submitting Site:
INIS
Size:
Pages: 7-11
Announcement Date:
Sep 01, 1976

Journal Article:

Citation Formats

Gylfe, E, and Sehlin, J. Interactions between the metabolism of L-leucine and D-glucose in the pancreatic. beta. -cells. Germany: N. p., 1976. Web.
Gylfe, E, & Sehlin, J. Interactions between the metabolism of L-leucine and D-glucose in the pancreatic. beta. -cells. Germany.
Gylfe, E, and Sehlin, J. 1976. "Interactions between the metabolism of L-leucine and D-glucose in the pancreatic. beta. -cells." Germany.
@misc{etde_7333960,
title = {Interactions between the metabolism of L-leucine and D-glucose in the pancreatic. beta. -cells}
author = {Gylfe, E, and Sehlin, J}
abstractNote = {..beta..-cell-rich pancreatic islets microdissected from obese-hyperglycemic mice were used to study interactions between the metabolism of L-leucine and D-glucose. L-leucine reduced the islet content of aspartic acid whereas D-glucose, when added to L-leucine-incubated islets, increased the contents of aspartic acid and ..gamma..-aminobutyric acid (GABA). D-glucose also increased the incorporation of L-leucine carbon into aspartic acid, GABA and glutamic acid, suggesting stimulation of a malate shuttle mechanism. When expressed per mole of the individual amino acids, the incorporation of L-leucine carbon into GABA was 2.5 - 4 times higher than into glutamic acid indicating intracellular compartmentation of the latter amino acid. Both L-leucine and D-leucine stimulated /sup 14/CO/sub 2/ production from /sup 14/C-labelled D-glucose. L-leucine did not affect /sup 3/H/sub 2/O production from tritiated D-glucose. The present data do not indicate a role of other amino acids or D-glucose in L-leucine-stimulated insulin release.}
journal = {Horm. Metab. Res.; (Germany, Federal Republic of)}
volume = {8:1}
journal type = {AC}
place = {Germany}
year = {1976}
month = {Jan}
}