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New solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody

Journal Article:

Abstract

A new competitive solid phase radioimmunoassay (CLB-RIA) has been developed for the detection of HBAg and HBAb in human serum and plasma. In the assay, sheep antibodies to HBAg, covalently linked to an insoluble carrier, highly purified /sup 125/I labelled HBAg and the serum or plasma sample are incubated for 20 h at room temperature. After incubation, the bound and the free fraction of the tracer are separated by centrifugation. The presence of both HBAg and HBAb results in a decrease of the amount of bound tracer, when compared with a negative control serum. Differentiation between HBAg and HBAb requires the use of another type of radioimmunoassay. For this purpose a sandwich solid phase radioimmunoassay, for the detection of HBAb only, has been developed (CLB-AURIA). In this, assay-purified HBAg is covalently linked to an insoluble carrier. Using a mixture of both immunosorbents (insolubilized HBAg and HBAb), it is possible to detect and to distinguish HBAg and HBAb in one single solid phase radioimmunoassay (CLB-MIRIA). The influence of three parameters on the CLB-RIA, the incubation time, the amount of tracer and the effect of Tween-20 has been studied. The sensitivity of the described solid phase CLB-RIA for the detection of HBAg  More>>
Authors:
Duimel, W J; [1]  Brummelhuis, H G.J.
  1. Centraal Laboratorium van de Bloedtransfusiedienst, Amsterdam
Publication Date:
Jul 01, 1975
Product Type:
Journal Article
Reference Number:
EDB-77-027180
Resource Relation:
Journal Name: Vox Sang.; (Switzerland); Journal Volume: 29:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIBODIES; RADIOIMMUNOASSAY; ANTIGENS; BLOOD PLASMA; BLOOD SERUM; HEPATITIS; IMMUNE SERUMS; IODINE 125; LABELLED COMPOUNDS; MAN; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM DISEASES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; PRIMATES; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7328106
Country of Origin:
Switzerland
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: VOSAA
Submitting Site:
TIC
Size:
Pages: 1-14
Announcement Date:
Feb 01, 1977

Journal Article:

Citation Formats

Duimel, W J, and Brummelhuis, H G.J. New solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody. Switzerland: N. p., 1975. Web.
Duimel, W J, & Brummelhuis, H G.J. New solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody. Switzerland.
Duimel, W J, and Brummelhuis, H G.J. 1975. "New solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody." Switzerland.
@misc{etde_7328106,
title = {New solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody}
author = {Duimel, W J, and Brummelhuis, H G.J.}
abstractNote = {A new competitive solid phase radioimmunoassay (CLB-RIA) has been developed for the detection of HBAg and HBAb in human serum and plasma. In the assay, sheep antibodies to HBAg, covalently linked to an insoluble carrier, highly purified /sup 125/I labelled HBAg and the serum or plasma sample are incubated for 20 h at room temperature. After incubation, the bound and the free fraction of the tracer are separated by centrifugation. The presence of both HBAg and HBAb results in a decrease of the amount of bound tracer, when compared with a negative control serum. Differentiation between HBAg and HBAb requires the use of another type of radioimmunoassay. For this purpose a sandwich solid phase radioimmunoassay, for the detection of HBAb only, has been developed (CLB-AURIA). In this, assay-purified HBAg is covalently linked to an insoluble carrier. Using a mixture of both immunosorbents (insolubilized HBAg and HBAb), it is possible to detect and to distinguish HBAg and HBAb in one single solid phase radioimmunoassay (CLB-MIRIA). The influence of three parameters on the CLB-RIA, the incubation time, the amount of tracer and the effect of Tween-20 has been studied. The sensitivity of the described solid phase CLB-RIA for the detection of HBAg is comparable to that of other radioimmunoassays reported in literature; its specificity is very high.}
journal = {Vox Sang.; (Switzerland)}
volume = {29:1}
journal type = {AC}
place = {Switzerland}
year = {1975}
month = {Jul}
}