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Photosynthetic carboxylating enzymes in Phaeodactylum tricornutum: assay methods and properties

Journal Article:

Abstract

Rapid freezing (in liquid nitrogen) of the marine diatom Phaeodactylum tricornutum Bohlin followed by thawing permits a convenient and sensitive measurement of the activities of carboxylating enzymes without the need to prepare a cell-free extract. Using this method, the properties of RuDP and PEP carboxylases have been compared with those assayed in cell-free extracts. The most significant difference was in the Michaelis' constants (K/sub m/'s), the values being lower in the freeze/thaw assay. The absolute rate of carbon-dioxide fixation by the enzymes was less than the rate of photosynthesis by the intact alga. Significantly, the activity of PEP carboxylase was comparable (in some experiments, greater) to that of RuDP carboxylase. The significance of this and the possibility of an enzymatic approach to measurements of marine primary productivity are discussed.
Authors:
Mukerji, D; [1]  Morris, I
  1. Bigelow Lab. for Ocean Sciences, West Boothbay Harbor, ME
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
EDB-77-063861
Resource Relation:
Journal Name: Mar. Biol.; (Germany, Federal Republic of); Journal Volume: 36:3
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CARBON DIOXIDE; METABOLISM; CARBOXYLASE; MEASURING METHODS; DIATOMS; PHOTOSYNTHESIS; BIOCHEMICAL REACTION KINETICS; ALGAE; BIOASSAY; CATALYSIS; ENZYMES; FREEZING; THAWING; BIOMASS; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; CHEMICAL REACTIONS; ENERGY SOURCES; KINETICS; LYASES; OXIDES; OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; REACTION KINETICS; RENEWABLE ENERGY SOURCES; SYNTHESIS; 550200* - Biochemistry; 560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)
OSTI ID:
7327994
Country of Origin:
Germany
Language:
English
Contract Number:
E(11-1)-2538
Other Identifying Numbers:
Journal ID: CODEN: MBIOA
Submitting Site:
TIC
Size:
Pages: 199-206
Announcement Date:

Journal Article:

Citation Formats

Mukerji, D, and Morris, I. Photosynthetic carboxylating enzymes in Phaeodactylum tricornutum: assay methods and properties. Germany: N. p., 1976. Web.
Mukerji, D, & Morris, I. Photosynthetic carboxylating enzymes in Phaeodactylum tricornutum: assay methods and properties. Germany.
Mukerji, D, and Morris, I. 1976. "Photosynthetic carboxylating enzymes in Phaeodactylum tricornutum: assay methods and properties." Germany.
@misc{etde_7327994,
title = {Photosynthetic carboxylating enzymes in Phaeodactylum tricornutum: assay methods and properties}
author = {Mukerji, D, and Morris, I}
abstractNote = {Rapid freezing (in liquid nitrogen) of the marine diatom Phaeodactylum tricornutum Bohlin followed by thawing permits a convenient and sensitive measurement of the activities of carboxylating enzymes without the need to prepare a cell-free extract. Using this method, the properties of RuDP and PEP carboxylases have been compared with those assayed in cell-free extracts. The most significant difference was in the Michaelis' constants (K/sub m/'s), the values being lower in the freeze/thaw assay. The absolute rate of carbon-dioxide fixation by the enzymes was less than the rate of photosynthesis by the intact alga. Significantly, the activity of PEP carboxylase was comparable (in some experiments, greater) to that of RuDP carboxylase. The significance of this and the possibility of an enzymatic approach to measurements of marine primary productivity are discussed.}
journal = {Mar. Biol.; (Germany, Federal Republic of)}
volume = {36:3}
journal type = {AC}
place = {Germany}
year = {1976}
month = {Jan}
}