You need JavaScript to view this

Radioimmunoassay for antibodies against surface membrane antigens using adhering cells

Journal Article:

Abstract

A radioimmunoassay using cells adhering to plastic is described. In this assay, A-10 mammary carcinoma attached to the surface of plastic in microtiter plates were permitted to bind antibody and the bound antibody was detected with purified rabbit /sup 125/I-antimouse-Fab. The bound radioactive material was eluted with glycine-HCl buffer (pH 2.5), and the acid eluates were counted in a gamma counter. This assay can be used to detect cytolic or noncytolic antibody to cell surface antigens in studies with any tumor or normal cell that will adhere to a solid surface.
Authors:
Tax, A; Manson, L A [1] 
  1. Wistar Inst. of Anatomy and Biology, Philadelphia, Pa. (USA)
Publication Date:
Jul 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-07-260396; EDB-77-070148
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 1:3-4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIBODIES; RADIOIMMUNOASSAY; ADHESION; ANIMAL CELLS; GLASS; MEASURING METHODS; ORGANIC POLYMERS; PLATES; TUMOR CELLS; ISOTOPE APPLICATIONS; ORGANIC COMPOUNDS; POLYMERS; RADIOASSAY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7325953
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 281-286
Announcement Date:

Journal Article:

Citation Formats

Tax, A, and Manson, L A. Radioimmunoassay for antibodies against surface membrane antigens using adhering cells. Netherlands: N. p., 1976. Web.
Tax, A, & Manson, L A. Radioimmunoassay for antibodies against surface membrane antigens using adhering cells. Netherlands.
Tax, A, and Manson, L A. 1976. "Radioimmunoassay for antibodies against surface membrane antigens using adhering cells." Netherlands.
@misc{etde_7325953,
title = {Radioimmunoassay for antibodies against surface membrane antigens using adhering cells}
author = {Tax, A, and Manson, L A}
abstractNote = {A radioimmunoassay using cells adhering to plastic is described. In this assay, A-10 mammary carcinoma attached to the surface of plastic in microtiter plates were permitted to bind antibody and the bound antibody was detected with purified rabbit /sup 125/I-antimouse-Fab. The bound radioactive material was eluted with glycine-HCl buffer (pH 2.5), and the acid eluates were counted in a gamma counter. This assay can be used to detect cytolic or noncytolic antibody to cell surface antigens in studies with any tumor or normal cell that will adhere to a solid surface.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {1:3-4}
journal type = {AC}
place = {Netherlands}
year = {1976}
month = {Jul}
}