Abstract
Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/ cells were cultivated in a synthetic glucose medium supplemented with 2 ..mu..g/ml thymine and 14 ..mu..g/ml tryptophan until the beginning of the exponential growth phase. After filtration and washing the cells were thymine starved for different periods of time. During short-term starvation (about 40 minutes) the cells did not die and the frequency of Trp/sup +/ revertants as determined on the synthetic glucose medium supplemented with 2 ..mu..g/ml thymine and 0.75 ..mu..g/ml tryptophan solidified with agar did not increase. From the 45th min of starvation cells died exponentially and at the same time the fraction of Trp/sup +/ revertants in the population increased. During short-term starvation the sensitivity of cells to ultraviolet radiation become enhanced, at the same time one could see an increase of frequency of ''mutation-frequency-decline''-stable ultraviolet induced Trp/sup +/ revertants. Is is supposed that short-term thymine starvation affects the coordination of the rec/sup +/ and polAl/sup +/ systems participating in the uvr/sup +/ dependent DNA repair synthesis in favour of the rec/sup +/ system, incidentally starvation may affect the error-free postreplication repair in which the products of the uvr/sup +/ and rec/sup +/ genes participate.
Balgavy, P;
Turek, R
[1]
- Slovenska Akademia Vied, Bratislava (Czechoslovakia). Vyskumny Ustav Onkologicky
Citation Formats
Balgavy, P, and Turek, R.
Influence of thymine starvation on UV mutability of Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/.
Serbia and Montenegro: N. p.,
1976.
Web.
Balgavy, P, & Turek, R.
Influence of thymine starvation on UV mutability of Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/.
Serbia and Montenegro.
Balgavy, P, and Turek, R.
1976.
"Influence of thymine starvation on UV mutability of Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/."
Serbia and Montenegro.
@misc{etde_7325863,
title = {Influence of thymine starvation on UV mutability of Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/}
author = {Balgavy, P, and Turek, R}
abstractNote = {Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/ cells were cultivated in a synthetic glucose medium supplemented with 2 ..mu..g/ml thymine and 14 ..mu..g/ml tryptophan until the beginning of the exponential growth phase. After filtration and washing the cells were thymine starved for different periods of time. During short-term starvation (about 40 minutes) the cells did not die and the frequency of Trp/sup +/ revertants as determined on the synthetic glucose medium supplemented with 2 ..mu..g/ml thymine and 0.75 ..mu..g/ml tryptophan solidified with agar did not increase. From the 45th min of starvation cells died exponentially and at the same time the fraction of Trp/sup +/ revertants in the population increased. During short-term starvation the sensitivity of cells to ultraviolet radiation become enhanced, at the same time one could see an increase of frequency of ''mutation-frequency-decline''-stable ultraviolet induced Trp/sup +/ revertants. Is is supposed that short-term thymine starvation affects the coordination of the rec/sup +/ and polAl/sup +/ systems participating in the uvr/sup +/ dependent DNA repair synthesis in favour of the rec/sup +/ system, incidentally starvation may affect the error-free postreplication repair in which the products of the uvr/sup +/ and rec/sup +/ genes participate.}
journal = []
volume = {31:6}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1976}
month = {Jan}
}
title = {Influence of thymine starvation on UV mutability of Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/}
author = {Balgavy, P, and Turek, R}
abstractNote = {Escherichia coli B/r Hcr/sup +/ thy/sup -/ trp/sup -/ cells were cultivated in a synthetic glucose medium supplemented with 2 ..mu..g/ml thymine and 14 ..mu..g/ml tryptophan until the beginning of the exponential growth phase. After filtration and washing the cells were thymine starved for different periods of time. During short-term starvation (about 40 minutes) the cells did not die and the frequency of Trp/sup +/ revertants as determined on the synthetic glucose medium supplemented with 2 ..mu..g/ml thymine and 0.75 ..mu..g/ml tryptophan solidified with agar did not increase. From the 45th min of starvation cells died exponentially and at the same time the fraction of Trp/sup +/ revertants in the population increased. During short-term starvation the sensitivity of cells to ultraviolet radiation become enhanced, at the same time one could see an increase of frequency of ''mutation-frequency-decline''-stable ultraviolet induced Trp/sup +/ revertants. Is is supposed that short-term thymine starvation affects the coordination of the rec/sup +/ and polAl/sup +/ systems participating in the uvr/sup +/ dependent DNA repair synthesis in favour of the rec/sup +/ system, incidentally starvation may affect the error-free postreplication repair in which the products of the uvr/sup +/ and rec/sup +/ genes participate.}
journal = []
volume = {31:6}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1976}
month = {Jan}
}