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Basic and clinical evaluation of thyroxine radioimmunoassay kit. I. T-4 RIA kit

Journal Article:

Abstract

The T/sub 4/ RIA kit is based on single antibody radioimmunoassay and utilizes a resin-sponge to separate free T/sub 4/-/sup 125/I from the incubation mixture. The use of the sodium salt of trichloroacetic acid (TBG inhibitor) permits direct radioimmunoassay of T/sub 4/ without a tedious extraction procedure. The T/sub 4/ RIA kit needs only 0.05 ml of patient serum and one hour of incubation to measure T/sub 4/. The TBG inhibitior was equivalent to 5.1 ..mu..g% of T/sub 4/ in displacing radioactive T/sub 4/ from TBG in a CPBA(Res-O-Mat T/sub 4/) system, and the TBG inhibitor volume of 0.5 ml (ten times of serum) was needed to measure a high T/sub 4/ concentration serum. The change of incubation temperature was more affected on the standard curve than that of incubation time. Correlation of the T/sub 4/ value between radioimmunoassay and CPBA in 69 patients was good (r=0.88612, p<0.01) and showed a slightly higher T/sub 4/ value when measured with radioimmunoassay than CPBA (linear regression function: Y=1.17506 + 0.95766X). Correlation of T/sub 4/ between Compu-curve and conventional standard curve was excellent (r=0.9995), but a slight difference was found in both low and high T/sub 4/ concentrations. In euthyroid patients without thyroid  More>>
Authors:
Bunko, H; Hisada, K [1] 
  1. Kanazawa Univ. (Japan). Hospital
Publication Date:
Feb 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-283758; EDB-77-038516
Resource Relation:
Journal Name: Radioisotopes (Tokyo); (Japan); Journal Volume: 25:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; THYROXINE; RADIOIMMUNOASSAY; BLOOD SERUM; INCUBATION; IODINE 125; RADIOPHARMACEUTICALS; AMINO ACIDS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; PEPTIDE HORMONES; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOISOTOPES; THYROID HORMONES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7324113
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: RAISA
Submitting Site:
INIS
Size:
Pages: 76-81
Announcement Date:
Feb 01, 1977

Journal Article:

Citation Formats

Bunko, H, and Hisada, K. Basic and clinical evaluation of thyroxine radioimmunoassay kit. I. T-4 RIA kit. Japan: N. p., 1976. Web.
Bunko, H, & Hisada, K. Basic and clinical evaluation of thyroxine radioimmunoassay kit. I. T-4 RIA kit. Japan.
Bunko, H, and Hisada, K. 1976. "Basic and clinical evaluation of thyroxine radioimmunoassay kit. I. T-4 RIA kit." Japan.
@misc{etde_7324113,
title = {Basic and clinical evaluation of thyroxine radioimmunoassay kit. I. T-4 RIA kit}
author = {Bunko, H, and Hisada, K}
abstractNote = {The T/sub 4/ RIA kit is based on single antibody radioimmunoassay and utilizes a resin-sponge to separate free T/sub 4/-/sup 125/I from the incubation mixture. The use of the sodium salt of trichloroacetic acid (TBG inhibitor) permits direct radioimmunoassay of T/sub 4/ without a tedious extraction procedure. The T/sub 4/ RIA kit needs only 0.05 ml of patient serum and one hour of incubation to measure T/sub 4/. The TBG inhibitior was equivalent to 5.1 ..mu..g% of T/sub 4/ in displacing radioactive T/sub 4/ from TBG in a CPBA(Res-O-Mat T/sub 4/) system, and the TBG inhibitor volume of 0.5 ml (ten times of serum) was needed to measure a high T/sub 4/ concentration serum. The change of incubation temperature was more affected on the standard curve than that of incubation time. Correlation of the T/sub 4/ value between radioimmunoassay and CPBA in 69 patients was good (r=0.88612, p<0.01) and showed a slightly higher T/sub 4/ value when measured with radioimmunoassay than CPBA (linear regression function: Y=1.17506 + 0.95766X). Correlation of T/sub 4/ between Compu-curve and conventional standard curve was excellent (r=0.9995), but a slight difference was found in both low and high T/sub 4/ concentrations. In euthyroid patients without thyroid diseases, T/sub 4/ concentration was 9.92 +- 2.66 (SD) ..mu..g% and the normal range was between 4.60 and 15.24 ..mu..g%. Reproducibility within assay was 9.9% (CV) and recovery rate was 70% when added 10 ..mu..g of T/sub 4/ and 86% when added 15 ..mu..g% of T/sub 4/. From these results, the authors concluded that radioimmunoassay of serum T/sub 4/ using the T/sub 4/ RIA kit would be a promising one, especially when measuring a large amount of samples per assays.}
journal = {Radioisotopes (Tokyo); (Japan)}
volume = {25:2}
journal type = {AC}
place = {Japan}
year = {1976}
month = {Feb}
}