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Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat

Journal Article:

Abstract

The proliferating granulocyte precursor pool of rat bone marrow was labelled during DNA synthesis by continuous infusion and by single injection of /sup 3/H-thymidine (/sup 3/H-TdR), as well as by single injection of /sup 125/I-iododeoxyuridine (/sup 125/I-UdR). The appearance of neutrophilic granulocytes in the blood stream after these various labelling procedures was studied by autoradiography. Labelling patterns of blood neutrophils were identical during continuous infusion and after single injection of /sup 3/H-TdR, and 100 percent labelling of the blood compartment was achieved. This result indicated reutilization of DNA catabolites to occur in granulocytopoiesis leading to continuous availability of /sup 3/H-labelled DNA precursors even after a single injection of /sup 3/H-TdR. Attempts to suppress reutilization of label by infusion of cold thymidine 1 h after injection of /sup 3/H-TdR were unsuccessful. However, a change in the labelling pattern of blood neutrophils was seen after single injection of /sup 125/I-UdR, a DNA precursor poorly reutilized in comparison to /sup 3/H-TdR. This result provided further evidence for reutilization of DNA catabolites by the cell system investigated. A comprehensive discussion of the results indicates that thymidinemonophosphate is the biochemical level of reutilization in granulocytopoiesis.
Authors:
Gerecke, D; Gross, R [1] 
  1. Koeln Univ. (Germany, F.R.). Medizinische Klinik
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-281290; EDB-77-038594
Resource Relation:
Journal Name: Scand. J. Haematol.; (Denmark); Journal Volume: 17
Subject:
59 BASIC BIOLOGICAL SCIENCES; LEUKOPOIESIS; DNA; IODINE 125; NEUTROPHILS; RATS; THYMIDINE; TRITIUM COMPOUNDS; URIDINE; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BLOOD FORMATION; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; LEUKOCYTES; MAMMALS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIOISOTOPES; RIBOSIDES; RODENTS; URACILS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
7324074
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: SJHAA
Submitting Site:
INIS
Size:
Pages: 132-142
Announcement Date:

Journal Article:

Citation Formats

Gerecke, D, and Gross, R. Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat. Denmark: N. p., 1976. Web.
Gerecke, D, & Gross, R. Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat. Denmark.
Gerecke, D, and Gross, R. 1976. "Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat." Denmark.
@misc{etde_7324074,
title = {Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat}
author = {Gerecke, D, and Gross, R}
abstractNote = {The proliferating granulocyte precursor pool of rat bone marrow was labelled during DNA synthesis by continuous infusion and by single injection of /sup 3/H-thymidine (/sup 3/H-TdR), as well as by single injection of /sup 125/I-iododeoxyuridine (/sup 125/I-UdR). The appearance of neutrophilic granulocytes in the blood stream after these various labelling procedures was studied by autoradiography. Labelling patterns of blood neutrophils were identical during continuous infusion and after single injection of /sup 3/H-TdR, and 100 percent labelling of the blood compartment was achieved. This result indicated reutilization of DNA catabolites to occur in granulocytopoiesis leading to continuous availability of /sup 3/H-labelled DNA precursors even after a single injection of /sup 3/H-TdR. Attempts to suppress reutilization of label by infusion of cold thymidine 1 h after injection of /sup 3/H-TdR were unsuccessful. However, a change in the labelling pattern of blood neutrophils was seen after single injection of /sup 125/I-UdR, a DNA precursor poorly reutilized in comparison to /sup 3/H-TdR. This result provided further evidence for reutilization of DNA catabolites by the cell system investigated. A comprehensive discussion of the results indicates that thymidinemonophosphate is the biochemical level of reutilization in granulocytopoiesis.}
journal = {Scand. J. Haematol.; (Denmark)}
volume = {17}
journal type = {AC}
place = {Denmark}
year = {1976}
month = {Jan}
}