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Leukemic transformation of donor spleen cells following their transplantation into supralethally irradiated mice with pre-existing viral leukemia. [X Radiation]

Journal Article:

Abstract

Fialkow et al. previously reported leukemia induction in donor-type cells after treating patients for acute lymphoblastic leukemia with total-body irradiation and hematopoietic cell transplantation. Utilizing a murine model and paralleling their treatment protocol, we have documented that induction of leukemia can occur in normal donor cells transplanted into Rauscher viral leukemic mice at 0, 1 and 2 days after irradiation. The induction of leukemia in the grafted cells was verified by: the occurrence of splenomegaly; and secondary spleen cell transplants, whereby the secondary donors were transplanted mice still alive at 30 days and the secondary recipients were normal unirradiated mice. The spleen weights of the grafted leukemic mice were found to be significantly greater than those of the controls and all secondary recipients that received spleen cells from the primary grafted leukemic mice also died of leukemia. Verification that the regenerating hematopoietic tissue was from donor (males) and not host source (females) was accomplished by spleen chromosome preparations taken from randomly selected mice at 14 and at 30 days after cell transplantation. In these preparations, the Y chromosome was clearly distinguishable on the basis of size, shape, and differential staining. The data indicate that induction of leukemia after whole-body irradiation  More>>
Publication Date:
Jan 01, 1974
Product Type:
Journal Article
Reference Number:
ERA-02-033255; EDB-77-083769
Resource Relation:
Journal Name: Exp. Hematol.; (Denmark); Journal Volume: 2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HEMATOPOIETIC SYSTEM; BIOLOGICAL REGENERATION; LEUKEMIA; SPLEEN; BIOLOGICAL RADIATION EFFECTS; SPLEEN CELLS; TRANSPLANTS; HETEROCHROMOSOMES; MICE; RADIOTHERAPY; SPLENOMEGALY; VIRUSES; WEIGHT; X RADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BODY; CHROMOSOMES; DISEASES; ELECTROMAGNETIC RADIATION; HEMIC DISEASES; IONIZING RADIATIONS; MAMMALS; MEDICINE; MICROORGANISMS; NEOPLASMS; NUCLEAR MEDICINE; ORGANS; PARASITES; PATHOLOGICAL CHANGES; RADIATION EFFECTS; RADIATIONS; RADIOLOGY; RECOVERY; RODENTS; SOMATIC CELLS; SYMPTOMS; THERAPY; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals
OSTI ID:
7317342
Research Organizations:
Allegheny General Hospital, Pittsburgh
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EXHEB
Submitting Site:
TIC
Size:
Pages: 328-335
Announcement Date:

Journal Article:

Citation Formats

Kuhnert, P M, OKunewick, J P, and Erhard, P. Leukemic transformation of donor spleen cells following their transplantation into supralethally irradiated mice with pre-existing viral leukemia. [X Radiation]. Denmark: N. p., 1974. Web.
Kuhnert, P M, OKunewick, J P, & Erhard, P. Leukemic transformation of donor spleen cells following their transplantation into supralethally irradiated mice with pre-existing viral leukemia. [X Radiation]. Denmark.
Kuhnert, P M, OKunewick, J P, and Erhard, P. 1974. "Leukemic transformation of donor spleen cells following their transplantation into supralethally irradiated mice with pre-existing viral leukemia. [X Radiation]." Denmark.
@misc{etde_7317342,
title = {Leukemic transformation of donor spleen cells following their transplantation into supralethally irradiated mice with pre-existing viral leukemia. [X Radiation]}
author = {Kuhnert, P M, OKunewick, J P, and Erhard, P}
abstractNote = {Fialkow et al. previously reported leukemia induction in donor-type cells after treating patients for acute lymphoblastic leukemia with total-body irradiation and hematopoietic cell transplantation. Utilizing a murine model and paralleling their treatment protocol, we have documented that induction of leukemia can occur in normal donor cells transplanted into Rauscher viral leukemic mice at 0, 1 and 2 days after irradiation. The induction of leukemia in the grafted cells was verified by: the occurrence of splenomegaly; and secondary spleen cell transplants, whereby the secondary donors were transplanted mice still alive at 30 days and the secondary recipients were normal unirradiated mice. The spleen weights of the grafted leukemic mice were found to be significantly greater than those of the controls and all secondary recipients that received spleen cells from the primary grafted leukemic mice also died of leukemia. Verification that the regenerating hematopoietic tissue was from donor (males) and not host source (females) was accomplished by spleen chromosome preparations taken from randomly selected mice at 14 and at 30 days after cell transplantation. In these preparations, the Y chromosome was clearly distinguishable on the basis of size, shape, and differential staining. The data indicate that induction of leukemia after whole-body irradiation and hematopoietic cell transplantation can occur in immunologically matched donor cells when a viral agent is present and that the incidence of this induction is not affected by a time delay between irradiation and transplant.}
journal = {Exp. Hematol.; (Denmark)}
volume = {2}
journal type = {AC}
place = {Denmark}
year = {1974}
month = {Jan}
}