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The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants

Abstract

The relationship between environmental stress and the concentration of certain organic compounds (carbohydrates, tannins and phenolics) in plant leaves is examined. Pyrolysis-gas chromatography-mass spectrometry (PY-GC-MS) can be a rapid and comprehensive method to profile the chemical composition of biomaterials. Enhanced structural information on biomolecules containing acid functional groups can be obtained by a modification of direct pyrolysis; simultaneous pyrolysis methylation (SPM). Initial investigation focused on the characterization of important classes of biomarkers in leaves and optimization of sample preparation and pyrolysis conditions. The pyrolytic techniques were examined as a tool to quantify the compositional changes in leaves of trees which have been subjected to different environmental stresses. Results are reported for three field applications: the effect of thinning on balsam fir trees; the effects of fertilization on kalmia plant; and the effect of ozone on white pine trees. It was found that certain pyrolysates of carbohydrates, tannins and of a dihydrochalcone were good indicators of fertilization and water stress. Two unique pyrolysate biomarkers were discovered in white pine needles as indications of ozone fumigation levels and ozone damage. 132 refs., 33 figs., 13 tabs.
Authors:
Publication Date:
Sep 01, 1993
Product Type:
Book
Reference Number:
CANM-94-0E9068; EDB-94-091358
Resource Relation:
Other Information: Thesis (M.Sc.)
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEAVES; BIOLOGICAL MARKERS; GAS CHROMATOGRAPHY; OZONE; BIOLOGICAL EFFECTS; PYROLYSIS; CHEMICAL REACTIONS; CHROMATOGRAPHY; DECOMPOSITION; SEPARATION PROCESSES; THERMOCHEMICAL PROCESSES; 560300* - Chemicals Metabolism & Toxicology
OSTI ID:
7308946
Research Organizations:
Memorial Univ. of Newfoundland, St. John's, NF (Canada)
Country of Origin:
Canada
Language:
English
Other Identifying Numbers:
Other: ISBN: 0-315-86687-X
Availability:
MF Micromedia Ltd., 240 Catherine Street, Suite 305, Ottawa, Ontario, Canada K2P 2G8 $15 CAN
Submitting Site:
CANM
Size:
Pages: (153 p)
Announcement Date:

Citation Formats

Zhang, Hongbing. The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants. Canada: N. p., 1993. Web.
Zhang, Hongbing. The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants. Canada.
Zhang, Hongbing. 1993. "The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants." Canada.
@misc{etde_7308946,
title = {The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants}
author = {Zhang, Hongbing}
abstractNote = {The relationship between environmental stress and the concentration of certain organic compounds (carbohydrates, tannins and phenolics) in plant leaves is examined. Pyrolysis-gas chromatography-mass spectrometry (PY-GC-MS) can be a rapid and comprehensive method to profile the chemical composition of biomaterials. Enhanced structural information on biomolecules containing acid functional groups can be obtained by a modification of direct pyrolysis; simultaneous pyrolysis methylation (SPM). Initial investigation focused on the characterization of important classes of biomarkers in leaves and optimization of sample preparation and pyrolysis conditions. The pyrolytic techniques were examined as a tool to quantify the compositional changes in leaves of trees which have been subjected to different environmental stresses. Results are reported for three field applications: the effect of thinning on balsam fir trees; the effects of fertilization on kalmia plant; and the effect of ozone on white pine trees. It was found that certain pyrolysates of carbohydrates, tannins and of a dihydrochalcone were good indicators of fertilization and water stress. Two unique pyrolysate biomarkers were discovered in white pine needles as indications of ozone fumigation levels and ozone damage. 132 refs., 33 figs., 13 tabs.}
place = {Canada}
year = {1993}
month = {Sep}
}