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Depression of pyrimidine dimer excision from the aspects of uv reversibility of irradiated cells

Abstract

Depression of pyrimidine dimer excision induced in uv-irradiated E.coli B/r T/sup -/trp/sup -/Hcr/sup +/ cells by preirradiation cultivation in conditions of starving for the essential amino acid and thymine does not increase uv-reversibility of irradiated cells and does not influence the time of expression of trp/sup +/ reversions. The expression of mutations becomes completed in control and prestarved cells prior to restoration of postradiation division. Genetic deficiency leads up to their high sensitivity to the mutagenic activity of uv irradiation. Expression of trp/sup +/ revertants in Hcr/sup -/ type cells does not become completed until after commencement of the postradiation division of irradiated cells. Prestarved E.coli B/r T/sup -/trp/sup -/Hcr/sup +/ cells exhibited depression of excision even with postradiation cultivation in the absence of an essential amino acid, which is associated with greater stability of newly synthesized DNA and overall decrease of the death rate of cells. In postradiation starvation for the essential amino acid E.coli B/r T/sup -/trp/sup -/Hcr/sup -/ cells irradiated with low uv light doses behaved similarly. Control E.coli B/r T/sup -/trp/sup -/Hcr/sup +/ cells, cultivated after irradiation without amino acid, excised pyrimidine dimers; they are characterised by high degradation of newly synthesized DNA and increased death  More>>
Authors:
Slamenova, D; Slezarikova, V; Masek, F [1] 
  1. Slovenska Akademia Vied, Bratislava (Czechoslovakia)
Publication Date:
Apr 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-337537; EDB-77-148027
Resource Relation:
Journal Name: Stud. Biophys.; (German Democratic Republic); Journal Volume: 63:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; ESCHERICHIA COLI; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; DIMERS; FASTING; LETHAL MUTATIONS; THYMINE; TRYPTOPHAN; ULTRAVIOLET RADIATION; AMINO ACIDS; AZINES; AZOLES; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CARBOXYLIC ACIDS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; MICROORGANISMS; MUTATIONS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; PYRROLES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; URACILS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7298068
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: STBIB
Submitting Site:
INIS
Size:
Pages: 83-95
Announcement Date:
Oct 01, 1977

Citation Formats

Slamenova, D, Slezarikova, V, and Masek, F. Depression of pyrimidine dimer excision from the aspects of uv reversibility of irradiated cells. Germany: N. p., 1977. Web.
Slamenova, D, Slezarikova, V, & Masek, F. Depression of pyrimidine dimer excision from the aspects of uv reversibility of irradiated cells. Germany.
Slamenova, D, Slezarikova, V, and Masek, F. 1977. "Depression of pyrimidine dimer excision from the aspects of uv reversibility of irradiated cells." Germany.
@misc{etde_7298068,
title = {Depression of pyrimidine dimer excision from the aspects of uv reversibility of irradiated cells}
author = {Slamenova, D, Slezarikova, V, and Masek, F}
abstractNote = {Depression of pyrimidine dimer excision induced in uv-irradiated E.coli B/r T/sup -/trp/sup -/Hcr/sup +/ cells by preirradiation cultivation in conditions of starving for the essential amino acid and thymine does not increase uv-reversibility of irradiated cells and does not influence the time of expression of trp/sup +/ reversions. The expression of mutations becomes completed in control and prestarved cells prior to restoration of postradiation division. Genetic deficiency leads up to their high sensitivity to the mutagenic activity of uv irradiation. Expression of trp/sup +/ revertants in Hcr/sup -/ type cells does not become completed until after commencement of the postradiation division of irradiated cells. Prestarved E.coli B/r T/sup -/trp/sup -/Hcr/sup +/ cells exhibited depression of excision even with postradiation cultivation in the absence of an essential amino acid, which is associated with greater stability of newly synthesized DNA and overall decrease of the death rate of cells. In postradiation starvation for the essential amino acid E.coli B/r T/sup -/trp/sup -/Hcr/sup -/ cells irradiated with low uv light doses behaved similarly. Control E.coli B/r T/sup -/trp/sup -/Hcr/sup +/ cells, cultivated after irradiation without amino acid, excised pyrimidine dimers; they are characterised by high degradation of newly synthesized DNA and increased death rate of cells.}
journal = []
volume = {63:2}
journal type = {AC}
place = {Germany}
year = {1977}
month = {Apr}
}