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Estimation of urinary angiotensin II by radioimmunoassay

Journal Article:

Abstract

Urine samples were collected from fasting subjects after maintaining posture for 2 hr in early morning. Urinary angiotensin II was extracted with SE-Sephadex. The extracts, after being dissolved in phosphate buffer, pH 7.5, were measured by radioimmunoassay. Recovery, sensitivity and accuracy were found to be satisfactory. The normal values obtained from 6 subjects were 52-280 pg/2 hr. The values were almost normal in essential hypertension and in chronic glomerulonephritis. They were high in 3 out of 6 cases with renovascular hypertension and subsequently dropped after surgery. In 6 cases with primary aldosteronism, very low levels were found. These increased after the removal of adrenal adenomas. No positive correlation between simultaneous plasma and urinary angiotensin samples was apparent. Also no positive correlation between urinary angiotensin and urine volume was found. In renovascular hypertention, during glucose infusion, lower values in urine volume and angiotensin excretion were observed on the stenotic side as compared to the intact side. Thus, the angiotensin excretion rate does not appear to be regulated by arterial angiotensin concentration, but rather by the angiotensin perfusion rate.
Authors:
Fukuchi, S [1] 
  1. Tohoku Univ., Sendai (Japan). School of Medicine
Publication Date:
Nov 01, 1974
Product Type:
Journal Article
Reference Number:
EDB-76-038151
Resource Relation:
Journal Name: Tohoku J. Exp. Med.; (Japan); Journal Volume: 114:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANGIOTENSIN; RADIOIMMUNOASSAY; ADENOMAS; BLOOD PLASMA; ENDOCRINE DISEASES; EXCRETION; HYPERTENSION; IODINE 131; LABELLED COMPOUNDS; PATIENTS; RENIN; URINE; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BLOOD; BODY FLUIDS; CARDIOVASCULAR DISEASES; CLEARANCE; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ENZYMES; GLOBULINS; HYDROLASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HYDROLASES; PROTEINS; RADIOASSAY; RADIOISOTOPES; SYMPATHOMIMETICS; SYMPTOMS; TRACER TECHNIQUES; VASCULAR DISEASES; WASTES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7292965
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: TJEMA
Submitting Site:
INIS
Size:
Pages: 205-213
Announcement Date:

Journal Article:

Citation Formats

Fukuchi, S. Estimation of urinary angiotensin II by radioimmunoassay. Japan: N. p., 1974. Web.
Fukuchi, S. Estimation of urinary angiotensin II by radioimmunoassay. Japan.
Fukuchi, S. 1974. "Estimation of urinary angiotensin II by radioimmunoassay." Japan.
@misc{etde_7292965,
title = {Estimation of urinary angiotensin II by radioimmunoassay}
author = {Fukuchi, S}
abstractNote = {Urine samples were collected from fasting subjects after maintaining posture for 2 hr in early morning. Urinary angiotensin II was extracted with SE-Sephadex. The extracts, after being dissolved in phosphate buffer, pH 7.5, were measured by radioimmunoassay. Recovery, sensitivity and accuracy were found to be satisfactory. The normal values obtained from 6 subjects were 52-280 pg/2 hr. The values were almost normal in essential hypertension and in chronic glomerulonephritis. They were high in 3 out of 6 cases with renovascular hypertension and subsequently dropped after surgery. In 6 cases with primary aldosteronism, very low levels were found. These increased after the removal of adrenal adenomas. No positive correlation between simultaneous plasma and urinary angiotensin samples was apparent. Also no positive correlation between urinary angiotensin and urine volume was found. In renovascular hypertention, during glucose infusion, lower values in urine volume and angiotensin excretion were observed on the stenotic side as compared to the intact side. Thus, the angiotensin excretion rate does not appear to be regulated by arterial angiotensin concentration, but rather by the angiotensin perfusion rate.}
journal = {Tohoku J. Exp. Med.; (Japan)}
volume = {114:3}
journal type = {AC}
place = {Japan}
year = {1974}
month = {Nov}
}