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Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide

Journal Article:

Abstract

The concept that antisera for radioimmunoassay can be improved by treatment with NaI and dialysis is challenged. Some antisera are little affected by NaI. Even when NaI does dissociate endogenous antigenic material from crude antiserum, in the subsequent dialysis NaI is dialysed at a much faster rate than antigen, allowing reassociation to occur. The reported improvement of antisera by the NaI method cannot be attributed to the effect of NaI but can be explained by the dilution which occurs during dialysis.
Authors:
Skrabanek, P; Powell, D; [1]  Kirrane, J [2] 
  1. Mater Misericordiae Hospital, Dublin (Ireland). Dept. of Endocrinology
  2. Mater Misericordiae Hospital, Dublin (Ireland). Dept. of Immunology
Publication Date:
Aug 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-337793; EDB-78-011051
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 16:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; IMMUNE SERUMS; DIALYSIS; CHEMICAL BONDS; CHEMICAL PREPARATION; IODINE 125; LABELLED COMPOUNDS; RADIOIMMUNOASSAY; SODIUM IODIDES; ALKALI METAL COMPOUNDS; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HALIDES; HALOGEN COMPOUNDS; INORGANIC PHOSPHORS; INTERMEDIATE MASS NUCLEI; IODIDES; IODINE COMPOUNDS; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; PHOSPHORS; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOISOTOPES; SEPARATION PROCESSES; SODIUM COMPOUNDS; SYNTHESIS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7282306
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 331-335
Announcement Date:
Oct 01, 1977

Journal Article:

Citation Formats

Skrabanek, P, Powell, D, and Kirrane, J. Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide. Netherlands: N. p., 1977. Web.
Skrabanek, P, Powell, D, & Kirrane, J. Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide. Netherlands.
Skrabanek, P, Powell, D, and Kirrane, J. 1977. "Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide." Netherlands.
@misc{etde_7282306,
title = {Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide}
author = {Skrabanek, P, Powell, D, and Kirrane, J}
abstractNote = {The concept that antisera for radioimmunoassay can be improved by treatment with NaI and dialysis is challenged. Some antisera are little affected by NaI. Even when NaI does dissociate endogenous antigenic material from crude antiserum, in the subsequent dialysis NaI is dialysed at a much faster rate than antigen, allowing reassociation to occur. The reported improvement of antisera by the NaI method cannot be attributed to the effect of NaI but can be explained by the dilution which occurs during dialysis.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {16:4}
journal type = {AC}
place = {Netherlands}
year = {1977}
month = {Aug}
}