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Study of subcellular distribution of /sup 67/Ga in tumor and liver

Journal Article:

Abstract

The following animals and transplanted tumors were used: rats implanted with Yoshida sarcoma and hepatoma AH109A, and mice implanted with Ehrlich tumor. /sup 67/Ga-citrate was injected into the rats intravenously and into the mice intraperitoneally. Ten minutes to 48 hours after the administration of /sup 67/Ga-citrate, the animals were sacrificed, and the tumor tissues and liver were excised. Subcellular fractionation of tumor tissues and livers was carried out according to the method of Hogeboom and Schneider. Radioactivity of each fraction was counted with a well type scintillation counter, and the protein of each fraction was measured according to Lowry's method. In Yoshida sarcoma and Ehrlich tumor, most of the radioactivity was localized in the supernatant fraction, and a small amount of radioactivity was localized in the mitochondrial fraction (lysosome contains in this fraction). But in the liver, most of the radioactivity was concentrated in the mitochondrial fraction, and the radioactivity of this fraction was increased with the passage of time after administration. Twenty-four hours later, about 50% of the total radioactivity was accumulated in this fraction. In the case of hepatoma AH109A, radioactivity of the mitochondrial fraction was increased with the passage of time after administration, and about 30% of  More>>
Authors:
Ando, A; Takeshita, M; Hiraki, T; [1]  Ando, T; Hisada, K
  1. Kanazawa Univ. (Japan). School of Paramedicine
Publication Date:
Feb 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-332470; EDB-78-011354
Resource Relation:
Journal Name: Radioisotopes (Tokyo); (Japan); Journal Volume: 26:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; EHRLICH ASCITES TUMOR; RADIONUCLIDE KINETICS; EXPERIMENTAL NEOPLASMS; GALLIUM 67; BIOLOGICAL LOCALIZATION; LIVER; ASCITES TUMOR CELLS; CITRATES; LYSOSOMES; MICE; MITOCHONDRIA; PROTEINS; RADIOPHARMACEUTICALS; RATS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACID SALTS; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GALLIUM ISOTOPES; GLANDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; RADIOACTIVE MATERIALS; RADIOISOTOPES; RODENTS; TUMOR CELLS; VERTEBRATES; 560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)
OSTI ID:
7282062
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: RAISA
Submitting Site:
INIS
Size:
Pages: 86-91
Announcement Date:

Journal Article:

Citation Formats

Ando, A, Takeshita, M, Hiraki, T, Ando, T, and Hisada, K. Study of subcellular distribution of /sup 67/Ga in tumor and liver. Japan: N. p., 1977. Web.
Ando, A, Takeshita, M, Hiraki, T, Ando, T, & Hisada, K. Study of subcellular distribution of /sup 67/Ga in tumor and liver. Japan.
Ando, A, Takeshita, M, Hiraki, T, Ando, T, and Hisada, K. 1977. "Study of subcellular distribution of /sup 67/Ga in tumor and liver." Japan.
@misc{etde_7282062,
title = {Study of subcellular distribution of /sup 67/Ga in tumor and liver}
author = {Ando, A, Takeshita, M, Hiraki, T, Ando, T, and Hisada, K}
abstractNote = {The following animals and transplanted tumors were used: rats implanted with Yoshida sarcoma and hepatoma AH109A, and mice implanted with Ehrlich tumor. /sup 67/Ga-citrate was injected into the rats intravenously and into the mice intraperitoneally. Ten minutes to 48 hours after the administration of /sup 67/Ga-citrate, the animals were sacrificed, and the tumor tissues and liver were excised. Subcellular fractionation of tumor tissues and livers was carried out according to the method of Hogeboom and Schneider. Radioactivity of each fraction was counted with a well type scintillation counter, and the protein of each fraction was measured according to Lowry's method. In Yoshida sarcoma and Ehrlich tumor, most of the radioactivity was localized in the supernatant fraction, and a small amount of radioactivity was localized in the mitochondrial fraction (lysosome contains in this fraction). But in the liver, most of the radioactivity was concentrated in the mitochondrial fraction, and the radioactivity of this fraction was increased with the passage of time after administration. Twenty-four hours later, about 50% of the total radioactivity was accumulated in this fraction. In the case of hepatoma AH109A, radioactivity of the mitochondrial fraction was increased with the passage of time after administration, and about 30% of total activity was concentrated in this fraction at 24 hours after administration. From these results it is concluded that the lysosome does not play an important role in the concentration of /sup 67/Ga in the tumor, but that the lysosome plays an important role in the concentration of /sup 67/Ga in the liver. In the case of hepatoma AH109A, it is presumed that the lysosome plays a very important role in the concentration of /sup 67/Ga in the tumor, hepatoma AH109A having some nature of liver.}
journal = {Radioisotopes (Tokyo); (Japan)}
volume = {26:2}
journal type = {AC}
place = {Japan}
year = {1977}
month = {Feb}
}