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Effect of ultraviolet exposure on mitochondrial respiratory system

Journal Article:

Abstract

To find the photodynamic effect of ultraviolet light on the mitochondrial respiratory chain, mitochondria were obtained from rat livers, and the suspension was exposed to an extensive ultraviolet light. The oxygen consumption was measured polarographically with a Clark oxygen electrode. The effect of ultraviolet exposure on the five states of respiratory control (Chance and Williams), the P/O ratio, and the respiratory control index in mitochondria was discussed. The ultraviolet light with a dose of 9.6 x 10/sup 6/ erg/cm/sup 2/ caused the oxidative phosphorylation in mitochondria to uncouple. The 2nd phosphorylation site of the respiratory chain was susceptible to ultraviolet exposure. The stimulation of latent ATPase activity in mitochondria following exposure was observed by increasing exposure of ultraviolet light. However, DNP-stimulated ATPase was found to be stable in activity. The uncoupling of the respiratory chain by ultraviolet exposure was not detected if the mitochondrial suspension was preincubated with bovine serum albumin before exposure. The changes in light absorption of the mitochondrial suspension were followed at 520 nm after exposure. A close correlation was found between the ultraviolet exposure and swelling in mitochondria. But, the reversing contraction was observed by adding ATP to the swelled mitochondria. The peroxide compound was formed  More>>
Authors:
Noda, K [1] 
  1. Kurume Univ., Fukuoka (Japan). School of Medicine
Publication Date:
Sep 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-268325; EDB-77-008950
Resource Relation:
Journal Name: Kurume Igakkai Zasshi; (Japan); Journal Volume: 38:8-9
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; RESPIRATION; BIOLOGICAL RADIATION EFFECTS; ATP; ATP-ASE; CATALYSIS; IRRADIATION; LIVER; METABOLISM; MITOCHONDRIA; NEAR ULTRAVIOLET RADIATION; OXIDATION; RATS; ANIMALS; BIOLOGICAL EFFECTS; BODY; CELL CONSTITUENTS; CHEMICAL REACTIONS; DIGESTIVE SYSTEM; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; GLANDS; HYDROLASES; MAMMALS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; PHOSPHATASES; RADIATION EFFECTS; RADIATIONS; RODENTS; ULTRAVIOLET RADIATION; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals
OSTI ID:
7250920
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: KIZAA
Submitting Site:
INIS
Size:
Pages: 879-896
Announcement Date:
Nov 01, 1976

Journal Article:

Citation Formats

Noda, K. Effect of ultraviolet exposure on mitochondrial respiratory system. Japan: N. p., 1975. Web.
Noda, K. Effect of ultraviolet exposure on mitochondrial respiratory system. Japan.
Noda, K. 1975. "Effect of ultraviolet exposure on mitochondrial respiratory system." Japan.
@misc{etde_7250920,
title = {Effect of ultraviolet exposure on mitochondrial respiratory system}
author = {Noda, K}
abstractNote = {To find the photodynamic effect of ultraviolet light on the mitochondrial respiratory chain, mitochondria were obtained from rat livers, and the suspension was exposed to an extensive ultraviolet light. The oxygen consumption was measured polarographically with a Clark oxygen electrode. The effect of ultraviolet exposure on the five states of respiratory control (Chance and Williams), the P/O ratio, and the respiratory control index in mitochondria was discussed. The ultraviolet light with a dose of 9.6 x 10/sup 6/ erg/cm/sup 2/ caused the oxidative phosphorylation in mitochondria to uncouple. The 2nd phosphorylation site of the respiratory chain was susceptible to ultraviolet exposure. The stimulation of latent ATPase activity in mitochondria following exposure was observed by increasing exposure of ultraviolet light. However, DNP-stimulated ATPase was found to be stable in activity. The uncoupling of the respiratory chain by ultraviolet exposure was not detected if the mitochondrial suspension was preincubated with bovine serum albumin before exposure. The changes in light absorption of the mitochondrial suspension were followed at 520 nm after exposure. A close correlation was found between the ultraviolet exposure and swelling in mitochondria. But, the reversing contraction was observed by adding ATP to the swelled mitochondria. The peroxide compound was formed in mitochondria irradiated with ultraviolet light. The amount of compounds formed was dependent on the radiant energy of ultraviolet light. The possible mechanisms involved in the photodynamic effect of ultraviolet light to the mitochondrial respiration system were discussed.}
journal = {Kurume Igakkai Zasshi; (Japan)}
volume = {38:8-9}
journal type = {AC}
place = {Japan}
year = {1975}
month = {Sep}
}