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In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation

Journal Article:

Abstract

Near-ultraviolet (near-UV; 320 to 405 nm) irradiation of Escherichia coli B/r induced the formation in vivo of /sup 4/Srd-Cyd adducts in transfer RNA, as evidenced by (1) fluorescence spectrum changes of tRNA extracted from irradiated cells and reduced with NaBH/sub 4/, (2) thin-layer chromatography on cellulose of hydrolysates of trichloroacetic acid-precipitable extracts of irradiated cells, and (3) comparison of these findings with adduct formation induced by near-UV irradiation of purified mixed tRNA from E.coli. The kinetics of induction of the /sup 4/Srd-Cyd adduct in vivo, and the near-UV fluences required, provided strong support for our earlier hypothesis that formation of these adducts was responsible for near-UV-induced growth delay in E.coli.
Authors:
Ramabhadran, T V; Fossum, T; Jagger, J [1] 
  1. Texas Univ., Dallas (USA)
Publication Date:
May 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-07-260064; EDB-77-014537
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 23:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; TRANSFER RNA; ADDUCTS; ESCHERICHIA COLI; BIOLOGICAL RADIATION EFFECTS; AZINES; BACTERIA; BIOLOGICAL EFFECTS; CHEMISTRY; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; KINETICS; MICROORGANISMS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; REACTION KINETICS; RIBOSIDES; RNA; SENSITIVITY; ULTRAVIOLET RADIATION; URACILS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7248496
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 315-321
Announcement Date:

Journal Article:

Citation Formats

Ramabhadran, T V, Fossum, T, and Jagger, J. In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation. United Kingdom: N. p., 1976. Web.
Ramabhadran, T V, Fossum, T, & Jagger, J. In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation. United Kingdom.
Ramabhadran, T V, Fossum, T, and Jagger, J. 1976. "In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation." United Kingdom.
@misc{etde_7248496,
title = {In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation}
author = {Ramabhadran, T V, Fossum, T, and Jagger, J}
abstractNote = {Near-ultraviolet (near-UV; 320 to 405 nm) irradiation of Escherichia coli B/r induced the formation in vivo of /sup 4/Srd-Cyd adducts in transfer RNA, as evidenced by (1) fluorescence spectrum changes of tRNA extracted from irradiated cells and reduced with NaBH/sub 4/, (2) thin-layer chromatography on cellulose of hydrolysates of trichloroacetic acid-precipitable extracts of irradiated cells, and (3) comparison of these findings with adduct formation induced by near-UV irradiation of purified mixed tRNA from E.coli. The kinetics of induction of the /sup 4/Srd-Cyd adduct in vivo, and the near-UV fluences required, provided strong support for our earlier hypothesis that formation of these adducts was responsible for near-UV-induced growth delay in E.coli.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {23:5}
journal type = {AC}
place = {United Kingdom}
year = {1976}
month = {May}
}