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Phytohemagglutinin (PHA) stimulation of peripheral-blood lymphocytes and stem cell take

Abstract

The effect of PHA-stimulation of peripheral-blood lymphocytes on the spleen-colony formation in irradiated rats was examined. 25-day old Wistar rats underwent total-body irradiation (600 R), and they were used as recipients. On the other hand, 2 and /sup 1///sub 2/ month old untreated Wistar rats were used as donors of peripheral-blood lymphocytes, which were obtained by sedimentation with Dextraven from defibrinated blood. Four rat lots were used. The 1st one did not receive irradiation, and was kept as ''blank control.'' The 2nd one was just irradiated and kept as ''radiated control.'' The 3rd and the 4th rat lots of the series were irradiated, but the former lot was injected i.v. with 5 x 10/sup 7/ peripheral-blood untreated lymphocytes, whereas the fourth lot was injected i.v. with the same amount of lymphocytes, which were previously incubated in vitro for 24 hrs with PHA-M (Difco). The results showed that the PHA-incubation of transplanted peripheral-blood lymphocytes significantly increases the number and size of the macroscopic spleen colonies, in relationship to the colonies which occurs after transplantation of untreated lymphocytes. Histo-cytological observation clearly showed that the colonies formed after injection of mitogen-pretreated peripheral-blood lymphocytes were predominantly of erythroid type and, then, of non-differentiated cells.  More>>
Authors:
Astaldi, G; [1]  Karanovic, D; Vettori, P P; Karanovic, J; Piletic, O
  1. Blood Research Foundation Center, Tortona, Italy
Publication Date:
Jan 01, 1974
Product Type:
Conference
Reference Number:
EDB-77-014646
Resource Relation:
Journal Name: Boll. Ist. Sieroterap. Milan.; (Italy); Journal Volume: 53:5; Conference: International conference on leukemia and aplastic anemia, Naples, Italy, 16 Sep 1974
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; IMMUNE REACTIONS; BIOLOGICAL RADIATION EFFECTS; IMMUNOSUPPRESSION; RADIOINDUCTION; LYMPHOCYTES; PHYTOHEMAGGLUTININ; RATS; SPLEEN COLONY FORMATION; STEM CELLS; TRANSPLANTS; WHOLE-BODY IRRADIATION; X RADIATION; AGGLUTININS; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; COLONY FORMATION; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HEMAGGLUTININS; IONIZING RADIATIONS; IRRADIATION; LEUKOCYTES; MAMMALS; MUCOPROTEINS; ORGANIC COMPOUNDS; POLYSACCHARIDES; PROTEINS; RADIATION EFFECTS; RADIATIONS; RODENTS; SACCHARIDES; SOMATIC CELLS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals
OSTI ID:
7248437
Country of Origin:
Italy
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BISMA
Submitting Site:
TIC
Size:
Pages: 599-608
Announcement Date:
May 13, 2001

Citation Formats

Astaldi, G, Karanovic, D, Vettori, P P, Karanovic, J, and Piletic, O. Phytohemagglutinin (PHA) stimulation of peripheral-blood lymphocytes and stem cell take. Italy: N. p., 1974. Web.
Astaldi, G, Karanovic, D, Vettori, P P, Karanovic, J, & Piletic, O. Phytohemagglutinin (PHA) stimulation of peripheral-blood lymphocytes and stem cell take. Italy.
Astaldi, G, Karanovic, D, Vettori, P P, Karanovic, J, and Piletic, O. 1974. "Phytohemagglutinin (PHA) stimulation of peripheral-blood lymphocytes and stem cell take." Italy.
@misc{etde_7248437,
title = {Phytohemagglutinin (PHA) stimulation of peripheral-blood lymphocytes and stem cell take}
author = {Astaldi, G, Karanovic, D, Vettori, P P, Karanovic, J, and Piletic, O}
abstractNote = {The effect of PHA-stimulation of peripheral-blood lymphocytes on the spleen-colony formation in irradiated rats was examined. 25-day old Wistar rats underwent total-body irradiation (600 R), and they were used as recipients. On the other hand, 2 and /sup 1///sub 2/ month old untreated Wistar rats were used as donors of peripheral-blood lymphocytes, which were obtained by sedimentation with Dextraven from defibrinated blood. Four rat lots were used. The 1st one did not receive irradiation, and was kept as ''blank control.'' The 2nd one was just irradiated and kept as ''radiated control.'' The 3rd and the 4th rat lots of the series were irradiated, but the former lot was injected i.v. with 5 x 10/sup 7/ peripheral-blood untreated lymphocytes, whereas the fourth lot was injected i.v. with the same amount of lymphocytes, which were previously incubated in vitro for 24 hrs with PHA-M (Difco). The results showed that the PHA-incubation of transplanted peripheral-blood lymphocytes significantly increases the number and size of the macroscopic spleen colonies, in relationship to the colonies which occurs after transplantation of untreated lymphocytes. Histo-cytological observation clearly showed that the colonies formed after injection of mitogen-pretreated peripheral-blood lymphocytes were predominantly of erythroid type and, then, of non-differentiated cells. Only a few of them were of a mixed type, consisting of both undifferentiated cells and erythroid cells.}
journal = []
volume = {53:5}
place = {Italy}
year = {1974}
month = {Jan}
}