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Radioimmunoassay studies of intestinal calcium-binding protein in the pig. II. The distribution of intestinal CaBP in pig tissues

Journal Article:

Abstract

Using a specific radioimmunoassay for porcine intestinal calcium-binding protein (CaBP), we have measured the concentration of CaBP in the various tissues and organs of normal pigs. Intestinal CaBP was present in highest concentration in the upper small intestine, with lower concentrations in the distal small intestine. Intestinal CaBP was also found, in lower concentrations, in kidney, liver, thyroid, pancreas, and blood. In all other tissues, including parathyroid, bone, skeletal muscle, and brain, CaBP immunoreactivity was undetectable or less than in blood. The elution profile of calcium-binding activity and immunoreactivity from gel filtration analysis of kidney and parathyroid extracts suggest that the calcium-binding protein in the parathyroid gland, and the major calcium-binding protein(s) in the kidney, are chemically and immunochemically different from intestinal CaBP.
Authors:
Arnold, B M; Kuttner, M; Willis, D M; Hitchman, A J.W.; Harrison, J E; Murray, T M [1] 
  1. Toronto Univ., Ontario (Canada). Dept. of Medicine
Publication Date:
Dec 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-262965; EDB-77-020359
Resource Relation:
Journal Name: Can. J. Physiol. Pharmacol.; (Canada); Journal Volume: 53:6; Other Information: 12 refs
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PROTEINS; RADIOIMMUNOASSAY; CALCIUM; INTESTINES; IODINE 125; IODINE 131; SWINE; ALKALINE EARTH METALS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; GASTROINTESTINAL TRACT; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7245625
Country of Origin:
Canada
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CJPPA
Submitting Site:
INIS
Size:
Pages: 1135-1140
Announcement Date:

Journal Article:

Citation Formats

Arnold, B M, Kuttner, M, Willis, D M, Hitchman, A J.W., Harrison, J E, and Murray, T M. Radioimmunoassay studies of intestinal calcium-binding protein in the pig. II. The distribution of intestinal CaBP in pig tissues. Canada: N. p., 1975. Web.
Arnold, B M, Kuttner, M, Willis, D M, Hitchman, A J.W., Harrison, J E, & Murray, T M. Radioimmunoassay studies of intestinal calcium-binding protein in the pig. II. The distribution of intestinal CaBP in pig tissues. Canada.
Arnold, B M, Kuttner, M, Willis, D M, Hitchman, A J.W., Harrison, J E, and Murray, T M. 1975. "Radioimmunoassay studies of intestinal calcium-binding protein in the pig. II. The distribution of intestinal CaBP in pig tissues." Canada.
@misc{etde_7245625,
title = {Radioimmunoassay studies of intestinal calcium-binding protein in the pig. II. The distribution of intestinal CaBP in pig tissues}
author = {Arnold, B M, Kuttner, M, Willis, D M, Hitchman, A J.W., Harrison, J E, and Murray, T M}
abstractNote = {Using a specific radioimmunoassay for porcine intestinal calcium-binding protein (CaBP), we have measured the concentration of CaBP in the various tissues and organs of normal pigs. Intestinal CaBP was present in highest concentration in the upper small intestine, with lower concentrations in the distal small intestine. Intestinal CaBP was also found, in lower concentrations, in kidney, liver, thyroid, pancreas, and blood. In all other tissues, including parathyroid, bone, skeletal muscle, and brain, CaBP immunoreactivity was undetectable or less than in blood. The elution profile of calcium-binding activity and immunoreactivity from gel filtration analysis of kidney and parathyroid extracts suggest that the calcium-binding protein in the parathyroid gland, and the major calcium-binding protein(s) in the kidney, are chemically and immunochemically different from intestinal CaBP.}
journal = {Can. J. Physiol. Pharmacol.; (Canada)}
volume = {53:6}
journal type = {AC}
place = {Canada}
year = {1975}
month = {Dec}
}