You need JavaScript to view this

Heterogeneity of protein hormones

Conference:

Abstract

Radioimmunoassay measures antigenic determinants of hormonal molecules in the plasmas and tissues. These estimations carried out after fractionation in biological fluids, have revealed several immunological forms of the same hormone. The main problem is in the relationship of the various immunoreactive forms to the same hormonal sequence. The similar immunoreactive forms of high molecular weight usually have low biological activity and suggest the presence of prohormone; the suggestion of prohormonal nature depends on the chronology of the incorporation of labelled leucine and enzymatic transformation of prohormone with low biological into active hormone. The forms with high molecular weight and similar immunological activity may be of another nature. Thus, it has been shown that the biosynthetic nature of a compound such as big big insulin in the rat is doubtful owing to the absence of specific incorporation of labelled leucine into the immunoprecipitate of this fraction. The significance of low molecular weight form is still little known. An example of these forms is supplied by the existence of an alpha sub-unit of gonadotrophin present in the plasma of menopausal women. The interest of analytical methods by radio-receptor, simulation of cyclase activity in the identification of biological activity of immunoreactive forms, is  More>>
Authors:
Rosselin, G; Bataille, D; Laburthe, M; Duran-Garcia, S [1] 
  1. Institut National de la Sante et de la Recherche Medicale (INSERM), Hopital Saint-Antoine, 75 - Paris (France)
Publication Date:
Dec 01, 1975
Product Type:
Conference
Reference Number:
AIX-07-260385; EDB-77-032835
Resource Relation:
Journal Name: Pathol.-Biol.; (France); Journal Volume: 23:10; Conference: 2. Colloquium of radioimmunology, Lyon, France, 25 Oct 1974
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PEPTIDE HORMONES; RADIOIMMUNOASSAY; ANTIGEN-ANTIBODY REACTIONS; BIOCHEMISTRY; BLOOD PLASMA; MOLECULAR STRUCTURE; POLYPEPTIDES; RATS; TISSUES; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BODY; BODY FLUIDS; CHEMISTRY; HORMONES; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC COMPOUNDS; PEPTIDES; PROTEINS; RADIOASSAY; RODENTS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7238666
Country of Origin:
France
Language:
French
Other Identifying Numbers:
Journal ID: CODEN: PABIA
Submitting Site:
INIS
Size:
Pages: 793-803
Announcement Date:
Oct 01, 1976

Conference:

Citation Formats

Rosselin, G, Bataille, D, Laburthe, M, and Duran-Garcia, S. Heterogeneity of protein hormones. France: N. p., 1975. Web.
Rosselin, G, Bataille, D, Laburthe, M, & Duran-Garcia, S. Heterogeneity of protein hormones. France.
Rosselin, G, Bataille, D, Laburthe, M, and Duran-Garcia, S. 1975. "Heterogeneity of protein hormones." France.
@misc{etde_7238666,
title = {Heterogeneity of protein hormones}
author = {Rosselin, G, Bataille, D, Laburthe, M, and Duran-Garcia, S}
abstractNote = {Radioimmunoassay measures antigenic determinants of hormonal molecules in the plasmas and tissues. These estimations carried out after fractionation in biological fluids, have revealed several immunological forms of the same hormone. The main problem is in the relationship of the various immunoreactive forms to the same hormonal sequence. The similar immunoreactive forms of high molecular weight usually have low biological activity and suggest the presence of prohormone; the suggestion of prohormonal nature depends on the chronology of the incorporation of labelled leucine and enzymatic transformation of prohormone with low biological into active hormone. The forms with high molecular weight and similar immunological activity may be of another nature. Thus, it has been shown that the biosynthetic nature of a compound such as big big insulin in the rat is doubtful owing to the absence of specific incorporation of labelled leucine into the immunoprecipitate of this fraction. The significance of low molecular weight form is still little known. An example of these forms is supplied by the existence of an alpha sub-unit of gonadotrophin present in the plasma of menopausal women. The interest of analytical methods by radio-receptor, simulation of cyclase activity in the identification of biological activity of immunoreactive forms, is discussed in relation to immunological forms ofenteroglucagon. An unusual aspect of the evolutive and adaptative character of hormonal heterogeneity is given by the gastro-intestinal hormones.}
journal = {Pathol.-Biol.; (France)}
volume = {23:10}
place = {France}
year = {1975}
month = {Dec}
}