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New /sup 125/I-anti-DNA-radioimmunoassay for the diagnosis of systematic Lupus erythematosus

Abstract

For a differential diagnosis distinguishing between systematic lupus erythematosus and progressive and chronic polyarthritis, a special RIA method has been developed and tested. The anti-DNA activity was determined as follows: the antigen was a high-molecular double strand DNA from a human tumour cell strain biologically labelled with /sup 125/I-desoxyuridine. Free and bound antigen was separated by precipitation using saturated ammonium sulfate solution. Recovery and interassay variance of this RIA are comparable with that of other RIAs.
Authors:
Neumeier, D; Vogt, W; Knedel, M [1] 
  1. Muenchen Univ. (F.R. Germany). Inst. fuer Klinische Chemie und Klinische Biochemie
Publication Date:
Apr 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-07-270756; EDB-77-038528
Resource Relation:
Journal Name: Z. Anal. Chem.; (Germany, Federal Republic of); Journal Volume: 279:2; Other Information: 1 fig.; 6 refs
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; DNA; RADIOIMMUNOASSAY; LUPUS; DIAGNOSTIC TECHNIQUES; ANTIGEN-ANTIBODY REACTIONS; BLOOD SERUM; DIGESTIVE SYSTEM DISEASES; FLUORESCENCE SPECTROSCOPY; IODINE 125; LIVER; NUCLEAR MEDICINE; PATIENTS; QUANTITATIVE CHEMICAL ANALYSIS; SKIN; URIDINE; AZINES; BETA DECAY RADIOISOTOPES; BODY; CHEMICAL ANALYSIS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EMISSION SPECTROSCOPY; GLANDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MEDICINE; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIOASSAY; RADIOISOTOPES; RIBOSIDES; SKIN DISEASES; SPECTROSCOPY; TRACER TECHNIQUES; TUBERCULOSIS; URACILS; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7236804
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: CODEN: ZACFA
Submitting Site:
INIS
Size:
Pages: 107-108
Announcement Date:
Dec 01, 1976

Citation Formats

Neumeier, D, Vogt, W, and Knedel, M. New /sup 125/I-anti-DNA-radioimmunoassay for the diagnosis of systematic Lupus erythematosus. Germany: N. p., 1976. Web.
Neumeier, D, Vogt, W, & Knedel, M. New /sup 125/I-anti-DNA-radioimmunoassay for the diagnosis of systematic Lupus erythematosus. Germany.
Neumeier, D, Vogt, W, and Knedel, M. 1976. "New /sup 125/I-anti-DNA-radioimmunoassay for the diagnosis of systematic Lupus erythematosus." Germany.
@misc{etde_7236804,
title = {New /sup 125/I-anti-DNA-radioimmunoassay for the diagnosis of systematic Lupus erythematosus}
author = {Neumeier, D, Vogt, W, and Knedel, M}
abstractNote = {For a differential diagnosis distinguishing between systematic lupus erythematosus and progressive and chronic polyarthritis, a special RIA method has been developed and tested. The anti-DNA activity was determined as follows: the antigen was a high-molecular double strand DNA from a human tumour cell strain biologically labelled with /sup 125/I-desoxyuridine. Free and bound antigen was separated by precipitation using saturated ammonium sulfate solution. Recovery and interassay variance of this RIA are comparable with that of other RIAs.}
journal = []
volume = {279:2}
journal type = {AC}
place = {Germany}
year = {1976}
month = {Apr}
}