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Single-strand breaks in the DNA of the uvrA and uvrB strains of Escherichia coli K-12 after ultraviolet irradiation

Abstract

DNA single-strand breaks were produced in uvrA and uvrB strains of E.coli K-12 after UV (254 nm) irradiation. These breaks appeared to be produced both directly by photochemical events, and by a temperature-dependent process. Cyclobutane-type pyrimidine dimers are probably not the photoproducts that lead to the temperature-dependent breaks, since photoreactivation had no detectable effect on the final yield of breaks. The DNA strand breaks appeared to be repairable by a process that requires DNA polymerase I and polynucleotide ligase, but not the recA, recB, recF, lexA101 or uvrD gene products. It is hypothesized that these temperature-dependent breaks occur either as a result of breakdown of a thermolabile photoproduct, or as the initial endonucleolytic event of a uvrA, uvrB-independent excision repair process that acts on a UV photoproduct other than the cyclobutane-type pyrimidine dimer.
Authors:
Youngs, D A; Smith, K C [1] 
  1. Stanford Univ., Calif. (USA). Dept. of Radiology
Publication Date:
Dec 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-298875; EDB-77-064132
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 24:6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BIOLOGICAL REPAIR; TEMPERATURE DEPENDENCE; DNA; STRAND BREAKS; CHEMICAL RADIATION EFFECTS; COMPARATIVE EVALUATIONS; DIMERS; ESCHERICHIA COLI; LIGASES; MUTANTS; NEAR ULTRAVIOLET RADIATION; PHOTOLYSIS; PHOTOREACTIVATION; PHOTOSENSITIVITY; POLYMERASES; PYRIMIDINES; AZINES; BACTERIA; BIOLOGICAL RECOVERY; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SENSITIVITY; ULTRAVIOLET RADIATION; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987)
OSTI ID:
7227743
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 533-541
Announcement Date:
Apr 01, 1977

Citation Formats

Youngs, D A, and Smith, K C. Single-strand breaks in the DNA of the uvrA and uvrB strains of Escherichia coli K-12 after ultraviolet irradiation. United Kingdom: N. p., 1976. Web.
Youngs, D A, & Smith, K C. Single-strand breaks in the DNA of the uvrA and uvrB strains of Escherichia coli K-12 after ultraviolet irradiation. United Kingdom.
Youngs, D A, and Smith, K C. 1976. "Single-strand breaks in the DNA of the uvrA and uvrB strains of Escherichia coli K-12 after ultraviolet irradiation." United Kingdom.
@misc{etde_7227743,
title = {Single-strand breaks in the DNA of the uvrA and uvrB strains of Escherichia coli K-12 after ultraviolet irradiation}
author = {Youngs, D A, and Smith, K C}
abstractNote = {DNA single-strand breaks were produced in uvrA and uvrB strains of E.coli K-12 after UV (254 nm) irradiation. These breaks appeared to be produced both directly by photochemical events, and by a temperature-dependent process. Cyclobutane-type pyrimidine dimers are probably not the photoproducts that lead to the temperature-dependent breaks, since photoreactivation had no detectable effect on the final yield of breaks. The DNA strand breaks appeared to be repairable by a process that requires DNA polymerase I and polynucleotide ligase, but not the recA, recB, recF, lexA101 or uvrD gene products. It is hypothesized that these temperature-dependent breaks occur either as a result of breakdown of a thermolabile photoproduct, or as the initial endonucleolytic event of a uvrA, uvrB-independent excision repair process that acts on a UV photoproduct other than the cyclobutane-type pyrimidine dimer.}
journal = []
volume = {24:6}
journal type = {AC}
place = {United Kingdom}
year = {1976}
month = {Dec}
}