Abstract
Ten UV-sensitive mutants of T4D with the v phenotype were isolated. Of these ten mutants, two are amber and two opal. In UV curves and in photoreactivation and multiplicity reactivation experiments the nonsense mutants show the v phenotype in su/sup -/ hosts and almost the T4/sup +/ phenotype in su/sup +/ hosts. The mutations are located between rl and e and are alleles of v/sub 1/. In crosses with irradiated and non-irradiated phages the recombinant frequency is not reduced by uvs5. Amber uvs5 propagated in CR63 su/sup +/ is with B su/sup -/ just as sensitive to UV as uvs5 propagated in B su/sup -/, which permits the conclusion that the capsid of T4 phage particles does not contain the v gene product.
Minderhout, L van;
Grimbergen, J;
Groot, B de;
[1]
Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands))
- Rijksuniversiteit Leiden (Netherlands). Lab. voor Stralengenetica en Chemische Mutagenese
Citation Formats
Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands)).
Nonsense mutants in the bacteriophage T4D v gene.
Netherlands: N. p.,
1975.
Web.
Minderhout, L van, Grimbergen, J, Groot, B de, & Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands)).
Nonsense mutants in the bacteriophage T4D v gene.
Netherlands.
Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands)).
1975.
"Nonsense mutants in the bacteriophage T4D v gene."
Netherlands.
@misc{etde_7226266,
title = {Nonsense mutants in the bacteriophage T4D v gene}
author = {Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands))}
abstractNote = {Ten UV-sensitive mutants of T4D with the v phenotype were isolated. Of these ten mutants, two are amber and two opal. In UV curves and in photoreactivation and multiplicity reactivation experiments the nonsense mutants show the v phenotype in su/sup -/ hosts and almost the T4/sup +/ phenotype in su/sup +/ hosts. The mutations are located between rl and e and are alleles of v/sub 1/. In crosses with irradiated and non-irradiated phages the recombinant frequency is not reduced by uvs5. Amber uvs5 propagated in CR63 su/sup +/ is with B su/sup -/ just as sensitive to UV as uvs5 propagated in B su/sup -/, which permits the conclusion that the capsid of T4 phage particles does not contain the v gene product.}
journal = []
volume = {29:3}
journal type = {AC}
place = {Netherlands}
year = {1975}
month = {Sep}
}
title = {Nonsense mutants in the bacteriophage T4D v gene}
author = {Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands))}
abstractNote = {Ten UV-sensitive mutants of T4D with the v phenotype were isolated. Of these ten mutants, two are amber and two opal. In UV curves and in photoreactivation and multiplicity reactivation experiments the nonsense mutants show the v phenotype in su/sup -/ hosts and almost the T4/sup +/ phenotype in su/sup +/ hosts. The mutations are located between rl and e and are alleles of v/sub 1/. In crosses with irradiated and non-irradiated phages the recombinant frequency is not reduced by uvs5. Amber uvs5 propagated in CR63 su/sup +/ is with B su/sup -/ just as sensitive to UV as uvs5 propagated in B su/sup -/, which permits the conclusion that the capsid of T4 phage particles does not contain the v gene product.}
journal = []
volume = {29:3}
journal type = {AC}
place = {Netherlands}
year = {1975}
month = {Sep}
}