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Nonsense mutants in the bacteriophage T4D v gene

Journal Article:

Abstract

Ten UV-sensitive mutants of T4D with the v phenotype were isolated. Of these ten mutants, two are amber and two opal. In UV curves and in photoreactivation and multiplicity reactivation experiments the nonsense mutants show the v phenotype in su/sup -/ hosts and almost the T4/sup +/ phenotype in su/sup +/ hosts. The mutations are located between rl and e and are alleles of v/sub 1/. In crosses with irradiated and non-irradiated phages the recombinant frequency is not reduced by uvs5. Amber uvs5 propagated in CR63 su/sup +/ is with B su/sup -/ just as sensitive to UV as uvs5 propagated in B su/sup -/, which permits the conclusion that the capsid of T4 phage particles does not contain the v gene product.
Authors:
Minderhout, L van; Grimbergen, J; Groot, B de; [1]  Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands))
  1. Rijksuniversiteit Leiden (Netherlands). Lab. voor Stralengenetica en Chemische Mutagenese
Publication Date:
Sep 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-240098; EDB-77-070284
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 29:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BACTERIOPHAGES; RADIOSENSITIVITY; COLOR; ESCHERICHIA COLI; GENE RECOMBINATION; GENES; MUTANTS; PHENOTYPE; PHOTOREACTIVATION; SURVIVAL CURVES; ULTRAVIOLET RADIATION; BACTERIA; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; ELECTROMAGNETIC RADIATION; MICROORGANISMS; OPTICAL PROPERTIES; ORGANOLEPTIC PROPERTIES; PARASITES; PHYSICAL PROPERTIES; RADIATIONS; RECOVERY; REPAIR; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7226266
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
INIS
Size:
Pages: 333-347
Announcement Date:

Journal Article:

Citation Formats

Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands)). Nonsense mutants in the bacteriophage T4D v gene. Netherlands: N. p., 1975. Web.
Minderhout, L van, Grimbergen, J, Groot, B de, & Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands)). Nonsense mutants in the bacteriophage T4D v gene. Netherlands.
Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands)). 1975. "Nonsense mutants in the bacteriophage T4D v gene." Netherlands.
@misc{etde_7226266,
title = {Nonsense mutants in the bacteriophage T4D v gene}
author = {Minderhout, L van, Grimbergen, J, Groot, B de, and Cohen (J.A.) Instituut voor Radiopathologie en Stralenbescherming, Leiden (Netherlands))}
abstractNote = {Ten UV-sensitive mutants of T4D with the v phenotype were isolated. Of these ten mutants, two are amber and two opal. In UV curves and in photoreactivation and multiplicity reactivation experiments the nonsense mutants show the v phenotype in su/sup -/ hosts and almost the T4/sup +/ phenotype in su/sup +/ hosts. The mutations are located between rl and e and are alleles of v/sub 1/. In crosses with irradiated and non-irradiated phages the recombinant frequency is not reduced by uvs5. Amber uvs5 propagated in CR63 su/sup +/ is with B su/sup -/ just as sensitive to UV as uvs5 propagated in B su/sup -/, which permits the conclusion that the capsid of T4 phage particles does not contain the v gene product.}
journal = {Mutat. Res.; (Netherlands)}
volume = {29:3}
journal type = {AC}
place = {Netherlands}
year = {1975}
month = {Sep}
}