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Influence of bromouracil density labelling on viability of UV irradiated Escherichia coli cells

Journal Article:

Abstract

Influence of 5-bromouracil cultivation on cell viability and DNA synthesis in the Escherichia coli B/r thy/sup -/ trp/sup -/ Hcr/sup +/ and Escherichia coli C thy-321 strains was followed. It was found that a 120 min cultivation in the bromouracil medium (unirradiated cells) does not essentially influence the viability of the two investigated strains but has an inhibitory effect on DNA synthesis in cells of the E. coli B/r Hcr/sup +/ strain. However, cultivation with bromouracil after ultraviolet irradiation leads to a decreased surviving ability of the irradiated cells of both investigated strains. Repair of damage induced by ultraviolet radiation probably exhausts a considerable proportion of repair activity, so that additional injury produced by bromouracil cultivation cannot be liquidated immediately.
Authors:
Brozmanova, J [1] 
  1. Slovenska Akademia Vied, Bratislava (Czechoslovakia). Vyskumny Ustav Onkologicky
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-286693; EDB-77-070292
Resource Relation:
Journal Name: Biologia (Bratislava); (Czechoslovakia); Journal Volume: 31:6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BROMOURACILS; RADIOSENSITIVITY EFFECTS; ESCHERICHIA COLI; BIOLOGICAL RADIATION EFFECTS; BIOSYNTHESIS; CARBON 14 COMPOUNDS; CULTURE MEDIA; DNA; DNA REPLICATION; LABELLED COMPOUNDS; SURVIVAL CURVES; THYMINE; TRACER TECHNIQUES; ULTRAVIOLET RADIATION; ANTIMETABOLITES; ANTIMITOTIC DRUGS; AZINES; BACTERIA; BIOLOGICAL EFFECTS; DRUGS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; MICROORGANISMS; NUCLEIC ACID REPLICATION; NUCLEIC ACIDS; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; SYNTHESIS; URACILS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7226264
Country of Origin:
Serbia and Montenegro
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BLOAA
Submitting Site:
INIS
Size:
Pages: 377-384
Announcement Date:

Journal Article:

Citation Formats

Brozmanova, J. Influence of bromouracil density labelling on viability of UV irradiated Escherichia coli cells. Serbia and Montenegro: N. p., 1976. Web.
Brozmanova, J. Influence of bromouracil density labelling on viability of UV irradiated Escherichia coli cells. Serbia and Montenegro.
Brozmanova, J. 1976. "Influence of bromouracil density labelling on viability of UV irradiated Escherichia coli cells." Serbia and Montenegro.
@misc{etde_7226264,
title = {Influence of bromouracil density labelling on viability of UV irradiated Escherichia coli cells}
author = {Brozmanova, J}
abstractNote = {Influence of 5-bromouracil cultivation on cell viability and DNA synthesis in the Escherichia coli B/r thy/sup -/ trp/sup -/ Hcr/sup +/ and Escherichia coli C thy-321 strains was followed. It was found that a 120 min cultivation in the bromouracil medium (unirradiated cells) does not essentially influence the viability of the two investigated strains but has an inhibitory effect on DNA synthesis in cells of the E. coli B/r Hcr/sup +/ strain. However, cultivation with bromouracil after ultraviolet irradiation leads to a decreased surviving ability of the irradiated cells of both investigated strains. Repair of damage induced by ultraviolet radiation probably exhausts a considerable proportion of repair activity, so that additional injury produced by bromouracil cultivation cannot be liquidated immediately.}
journal = {Biologia (Bratislava); (Czechoslovakia)}
volume = {31:6}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1976}
month = {Jan}
}