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Enriched reaction center preparation from green photosynthetic bacteria. [Chlorobium limicola]

Abstract

Bacteriochlorophyll a reaction-center complex I from Chlorobium limicola f. thiosulfatophilum 6230 (Tassajara) was incubated in 2 M guanidine . HCl and then chromatographed on cross-linked dextran or agarose gel. Two principal components were separated: a larger component with photochemical activity (bacteriochlorophyll a reaction-center complex II) and a smaller component without activity (bacteriochlorophyll a protein). Complex II contains carotenoid, bacteriochlorophyll a, reaction center(s), and cytochromes b and c, but lacks the well characterized bacteriochlorophyll a protein contained in Complex I. Complex II carries out a light-induced reduction of cytochrome b along with an oxidation of cytochrome c.
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
ERA-02-049886; EDB-77-108596
Resource Relation:
Journal Name: Biochim. Biophys. Acta; (Netherlands); Journal Volume: 449
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIA; PHOTOSYNTHESIS; CHLOROPHYLL; CHEMICAL COMPOSITION; CAROTENOIDS; CHROMATOGRAPHY; CYTOCHROMES; OXIDATION; PROTEINS; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PHYTOCHROMES; PIGMENTS; PORPHYRINS; SEPARATION PROCESSES; SYNTHESIS; TERPENES; 550200* - Biochemistry
OSTI ID:
7212944
Research Organizations:
Brookhaven National Lab., Upton, NY
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BBACA
Submitting Site:
BNL
Size:
Pages: 197-208
Announcement Date:
Aug 01, 1977

Citation Formats

Olson, J M, Giddings, Jr, T H, and Shaw, E K. Enriched reaction center preparation from green photosynthetic bacteria. [Chlorobium limicola]. Netherlands: N. p., 1976. Web. doi:10.1016/0005-2728(76)90133-X.
Olson, J M, Giddings, Jr, T H, & Shaw, E K. Enriched reaction center preparation from green photosynthetic bacteria. [Chlorobium limicola]. Netherlands. https://doi.org/10.1016/0005-2728(76)90133-X
Olson, J M, Giddings, Jr, T H, and Shaw, E K. 1976. "Enriched reaction center preparation from green photosynthetic bacteria. [Chlorobium limicola]." Netherlands. https://doi.org/10.1016/0005-2728(76)90133-X.
@misc{etde_7212944,
title = {Enriched reaction center preparation from green photosynthetic bacteria. [Chlorobium limicola]}
author = {Olson, J M, Giddings, Jr, T H, and Shaw, E K}
abstractNote = {Bacteriochlorophyll a reaction-center complex I from Chlorobium limicola f. thiosulfatophilum 6230 (Tassajara) was incubated in 2 M guanidine . HCl and then chromatographed on cross-linked dextran or agarose gel. Two principal components were separated: a larger component with photochemical activity (bacteriochlorophyll a reaction-center complex II) and a smaller component without activity (bacteriochlorophyll a protein). Complex II contains carotenoid, bacteriochlorophyll a, reaction center(s), and cytochromes b and c, but lacks the well characterized bacteriochlorophyll a protein contained in Complex I. Complex II carries out a light-induced reduction of cytochrome b along with an oxidation of cytochrome c.}
doi = {10.1016/0005-2728(76)90133-X}
journal = []
volume = {449}
journal type = {AC}
place = {Netherlands}
year = {1976}
month = {Jan}
}