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Effects of dexamethasone on brain edema. Uptake and distribution of tritiated (/sup 3/H) dexamethasone in cold induced edema

Journal Article:

Abstract

Experimental cerebral edema was produced on the right parietal lobe of Wistar male rats with a cold metal probe cooled by liquid nitrogen. Twenty hour later, /sup 3/H-dexamethasone was either intramuscularly or intravenously injected into rats, estimated in the brain tissue by the liquid scintillation counting method. Edematous brain generally contained much higher /sup 3/H-activity than the control. Furthermore, I.V. injection showed higher /sup 3/H-activity than I.M injection in edematous and control brains at all times. For examination of the subcellular distribution of /sup 3/H-dexamethasone in edematous brain, /sup 3/H-activity was most strongly detected in the supernatant fraction (63%), followed by the heavy mitochondrial fraction (25.4%) and the nuclear fraction (8.4%). Although edematous brain tissue constantly demonstrated higher /sup 3/H-activity than the control, its supernatant fraction conversely had less activity. As a next step, distribution of /sup 3/H-dexamethasone in the supernatant fraction was studies. The result was that the high molecular weight fraction in the edematous brain showed higher radioactivity than the control. From these findings, unequivocal distribution of dexamethasone in the supernatant fraction of edematous brain tissue could be correlated with its biochemical action for preventing brain edema.
Authors:
Takemoto, Motohisa [1] 
  1. Okayama Univ. (Japan). School of Medicine
Publication Date:
Jun 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-15-027938; EDB-84-095238
Resource Relation:
Journal Name: Okayama Igakkai Zasshi; (Japan); Journal Volume: 94:5-6
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; EDEMA; TRACER TECHNIQUES; CELL CULTURES; CENTRIFUGATION; COMPARATIVE EVALUATIONS; DEXAMETHASONE; DISTRIBUTION; INJURIES; INTRAMUSCULAR INJECTION; INTRAVENOUS INJECTION; QUANTITY RATIO; RATS; TIME DEPENDENCE; TRITIUM COMPOUNDS; UPTAKE; ADRENAL HORMONES; ANIMALS; BODY; CENTRAL NERVOUS SYSTEM; CORTICOSTEROIDS; GLUCOCORTICOIDS; HORMONES; HYDROXY COMPOUNDS; INJECTION; INTAKE; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; MAMMALS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PATHOLOGICAL CHANGES; PREGNANES; RODENTS; SEPARATION PROCESSES; STEROID HORMONES; STEROIDS; SYMPTOMS; VERTEBRATES; 550901* - Pathology- Tracer Techniques
OSTI ID:
7190434
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: OIZAA
Submitting Site:
HEDB
Size:
Pages: 487-502
Announcement Date:

Journal Article:

Citation Formats

Takemoto, Motohisa. Effects of dexamethasone on brain edema. Uptake and distribution of tritiated (/sup 3/H) dexamethasone in cold induced edema. Japan: N. p., 1982. Web.
Takemoto, Motohisa. Effects of dexamethasone on brain edema. Uptake and distribution of tritiated (/sup 3/H) dexamethasone in cold induced edema. Japan.
Takemoto, Motohisa. 1982. "Effects of dexamethasone on brain edema. Uptake and distribution of tritiated (/sup 3/H) dexamethasone in cold induced edema." Japan.
@misc{etde_7190434,
title = {Effects of dexamethasone on brain edema. Uptake and distribution of tritiated (/sup 3/H) dexamethasone in cold induced edema}
author = {Takemoto, Motohisa}
abstractNote = {Experimental cerebral edema was produced on the right parietal lobe of Wistar male rats with a cold metal probe cooled by liquid nitrogen. Twenty hour later, /sup 3/H-dexamethasone was either intramuscularly or intravenously injected into rats, estimated in the brain tissue by the liquid scintillation counting method. Edematous brain generally contained much higher /sup 3/H-activity than the control. Furthermore, I.V. injection showed higher /sup 3/H-activity than I.M injection in edematous and control brains at all times. For examination of the subcellular distribution of /sup 3/H-dexamethasone in edematous brain, /sup 3/H-activity was most strongly detected in the supernatant fraction (63%), followed by the heavy mitochondrial fraction (25.4%) and the nuclear fraction (8.4%). Although edematous brain tissue constantly demonstrated higher /sup 3/H-activity than the control, its supernatant fraction conversely had less activity. As a next step, distribution of /sup 3/H-dexamethasone in the supernatant fraction was studies. The result was that the high molecular weight fraction in the edematous brain showed higher radioactivity than the control. From these findings, unequivocal distribution of dexamethasone in the supernatant fraction of edematous brain tissue could be correlated with its biochemical action for preventing brain edema.}
journal = {Okayama Igakkai Zasshi; (Japan)}
volume = {94:5-6}
journal type = {AC}
place = {Japan}
year = {1982}
month = {Jun}
}