You need JavaScript to view this

Radioprotective effects of ascorbic acid in barley seeds

Abstract

Experiments were conducted to test the radioprotective effects of a naturally occurring reducing agent, ascorbic acid, on seeds (caryopses) of barley, Hordeum vulgare L. emend Lam. Seeds were soaked either before or after ..gamma.. or fission neutron irradiation in distilled water or ascorbic acid solutions ranging in concentration from 0.01 to 1.00 M. Results are reported as percentage germination, seedling height, seedling growth reduction, and (in one experiment) percent of cells with chromosome aberrations. As evidenced by both reduced germination and seedling growth, ascorbic acid was toxic when seeds were soaked for 1 hr at ambient temperature prior to irradiation and then planted immediately. When seeds were soaked in ascorbic acid before irradiation and soaked after irradiation in air-bubbled water at 0/sup 0/C for 18 hr, the toxicity disappeared, and a protective effect (which increased with increasing ascorbic acid concentration) was observed for ..gamma.. and, to a lesser extent, for neutron irradiation. Additional studies suggested that the protective effect was related to reduced hydration of the embryos of seeds soaked in ascorbic acid. Also, no radioprotective effect was observed when seeds were presoaked for 2 or 16 hr in 0.01 M ascorbic acid solutions buffered at pH 3 or pH  More>>
Authors:
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
ERA-01-021219; EDB-76-055740
Resource Relation:
Journal Name: Radiat. Bot.; (United Kingdom); Journal Volume: 15
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BARLEY; BIOLOGICAL RADIATION EFFECTS; FISSION NEUTRONS; GAMMA RADIATION; ASCORBIC ACID; CHROMOSOMAL ABERRATIONS; GERMINATION; PLANT GROWTH; RADIOPROTECTIVE SUBSTANCES; SEEDS; TOXICITY; BARYONS; BIOLOGICAL EFFECTS; BIOMASS; CEREALS; DRUGS; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; ENERGY SOURCES; FERMIONS; GRAMINEAE; GRASS; GROWTH; HADRONS; IONIZING RADIATIONS; MUTATIONS; NEUTRONS; NUCLEONS; PLANTS; RADIATION EFFECTS; RADIATIONS; RENEWABLE ENERGY SOURCES; RESPONSE MODIFYING FACTORS; VITAMINS; 560141* - Radiation Effects on Plants- Basic Studies- (-1987)
OSTI ID:
7190269
Research Organizations:
UT-AEC Comparative Animal Research Lab., Oak Ridge, TN
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: RABOA
Submitting Site:
TIC
Size:
Pages: 39-48
Announcement Date:
Aug 01, 1976

Citation Formats

Conger, B V. Radioprotective effects of ascorbic acid in barley seeds. United Kingdom: N. p., 1975. Web. doi:10.1016/S0033-7560(75)80013-5.
Conger, B V. Radioprotective effects of ascorbic acid in barley seeds. United Kingdom. doi:10.1016/S0033-7560(75)80013-5.
Conger, B V. 1975. "Radioprotective effects of ascorbic acid in barley seeds." United Kingdom. doi:10.1016/S0033-7560(75)80013-5. https://www.osti.gov/servlets/purl/10.1016/S0033-7560(75)80013-5.
@misc{etde_7190269,
title = {Radioprotective effects of ascorbic acid in barley seeds}
author = {Conger, B V}
abstractNote = {Experiments were conducted to test the radioprotective effects of a naturally occurring reducing agent, ascorbic acid, on seeds (caryopses) of barley, Hordeum vulgare L. emend Lam. Seeds were soaked either before or after ..gamma.. or fission neutron irradiation in distilled water or ascorbic acid solutions ranging in concentration from 0.01 to 1.00 M. Results are reported as percentage germination, seedling height, seedling growth reduction, and (in one experiment) percent of cells with chromosome aberrations. As evidenced by both reduced germination and seedling growth, ascorbic acid was toxic when seeds were soaked for 1 hr at ambient temperature prior to irradiation and then planted immediately. When seeds were soaked in ascorbic acid before irradiation and soaked after irradiation in air-bubbled water at 0/sup 0/C for 18 hr, the toxicity disappeared, and a protective effect (which increased with increasing ascorbic acid concentration) was observed for ..gamma.. and, to a lesser extent, for neutron irradiation. Additional studies suggested that the protective effect was related to reduced hydration of the embryos of seeds soaked in ascorbic acid. Also, no radioprotective effect was observed when seeds were presoaked for 2 or 16 hr in 0.01 M ascorbic acid solutions buffered at pH 3 or pH 7. A protective effect was observed for seeds of 1.5 percent water content soaked after irradiation in an oxygen-bubbled ascorbic acid solution of 0.5 M but was not observed for seeds soaked in nitrogen-bubbled ascorbic acid. The protective effect against oxygen-dependent damage may be a result of interaction of ascorbic acid with radiation-induced free radicals.}
doi = {10.1016/S0033-7560(75)80013-5}
journal = {Radiat. Bot.; (United Kingdom)}
volume = {15}
journal type = {AC}
place = {United Kingdom}
year = {1975}
month = {Jan}
}