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Demonstration of synthesis of beta-trace protein in different tissues of squirrel monkey

Abstract

The sites of synthesis of the low molwculat weight beta-trace protein, present in a seven times higher concentration in normal human CSF than in normal human serum, have been studied by means of a radioactive immunoprecipitation method. Adult squirrel monkey tissue were cultured in Eagle's minium essential medium in the presence of /sup 14/C-labelled valine, threonine and leucine for 24 hours. Synthesis could be demonstrated in cultures of white CNS matter, whereas cultures of grey CNS matter, peripheral nerve, skeletal muscle, kidney and ovary did not show any signs of synthesis. Some cultures of spinal cord, basal ganglia, genital organs except ovary, and liver showed a probable synthesis of beta-trace protein. By means of autoradiography, the synthesis of beta-trace protein in white CNS matter could be confirmed.
Authors:
Olsson, J E; Sandberg, M [1] 
  1. Department of Neurology, University Hospital, S-221 85 Lund, Sweden
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-242251; EDB-76-072297
Resource Relation:
Journal Name: Neurobiology; (Denmark); Journal Volume: 5
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEINS; BIOSYNTHESIS; BLOOD SERUM; CARBON 14 COMPOUNDS; LEUCINE; MONKEYS; THREONINE; TISSUE CULTURES; VALINE; AMINO ACIDS; ANIMALS; CARBOXYLIC ACIDS; HYDROXY ACIDS; LABELLED COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
7182009
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NBBMA
Submitting Site:
INIS
Size:
Pages: 270-276
Announcement Date:
Jul 01, 1976

Citation Formats

Olsson, J E, and Sandberg, M. Demonstration of synthesis of beta-trace protein in different tissues of squirrel monkey. Denmark: N. p., 1975. Web.
Olsson, J E, & Sandberg, M. Demonstration of synthesis of beta-trace protein in different tissues of squirrel monkey. Denmark.
Olsson, J E, and Sandberg, M. 1975. "Demonstration of synthesis of beta-trace protein in different tissues of squirrel monkey." Denmark.
@misc{etde_7182009,
title = {Demonstration of synthesis of beta-trace protein in different tissues of squirrel monkey}
author = {Olsson, J E, and Sandberg, M}
abstractNote = {The sites of synthesis of the low molwculat weight beta-trace protein, present in a seven times higher concentration in normal human CSF than in normal human serum, have been studied by means of a radioactive immunoprecipitation method. Adult squirrel monkey tissue were cultured in Eagle's minium essential medium in the presence of /sup 14/C-labelled valine, threonine and leucine for 24 hours. Synthesis could be demonstrated in cultures of white CNS matter, whereas cultures of grey CNS matter, peripheral nerve, skeletal muscle, kidney and ovary did not show any signs of synthesis. Some cultures of spinal cord, basal ganglia, genital organs except ovary, and liver showed a probable synthesis of beta-trace protein. By means of autoradiography, the synthesis of beta-trace protein in white CNS matter could be confirmed.}
journal = []
volume = {5}
journal type = {AC}
place = {Denmark}
year = {1975}
month = {Jan}
}