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Binding of a nitroxyl to radiation-induced DNA transients in repair and repair deficient of E. coli K-12

Journal Article:

Abstract

Binding of tritiated 2,2,6,6-tetramethyl-4-piperidone-N-oxyl (/sup 3/H-TAN) to radiation-induced DNA-transients in E. coli K-12 strains AB 1157 and JO 307 rec A uvr A has been studied under in vivo conditions. After irradiation the cells were washed and resuspended in growth medium and left overnight at 37 deg C. Within an uncertainty of about 10 %, no effect of repair could be detected on the yield of TAN bound to DNA for any of the strains. During the period after resuspension TAN or fragments of TAN leaked out of the irradiated cell samples. This leakage may be attributed to semi-permanent association between TAN and radiation-induced radicals within the cell. The relevance of different interactions between TAN and transients in DNA is discussed.
Authors:
Wold, E; Brustad, T [1] 
  1. Norsk Hydros Institutt for Kreftforskning, Oslo
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-242148; EDB-76-072431
Resource Relation:
Journal Name: Acta Chem. Scand., Ser. B; (Denmark); Journal Volume: 29:7; Other Information: 19 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; BIOLOGICAL RADIATION EFFECTS; ESCHERICHIA COLI; IN VIVO; PIPERIDINES; RADICALS; TRITIUM COMPOUNDS; AMINES; AZINES; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; HETEROCYCLIC COMPOUNDS; LABELLED COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIDINES; RADIATION EFFECTS; RECOVERY; REPAIR; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987); 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7181880
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACBOC
Submitting Site:
INIS
Size:
Pages: 781-786
Announcement Date:
Jul 01, 1976

Journal Article:

Citation Formats

Wold, E, and Brustad, T. Binding of a nitroxyl to radiation-induced DNA transients in repair and repair deficient of E. coli K-12. Denmark: N. p., 1975. Web.
Wold, E, & Brustad, T. Binding of a nitroxyl to radiation-induced DNA transients in repair and repair deficient of E. coli K-12. Denmark.
Wold, E, and Brustad, T. 1975. "Binding of a nitroxyl to radiation-induced DNA transients in repair and repair deficient of E. coli K-12." Denmark.
@misc{etde_7181880,
title = {Binding of a nitroxyl to radiation-induced DNA transients in repair and repair deficient of E. coli K-12}
author = {Wold, E, and Brustad, T}
abstractNote = {Binding of tritiated 2,2,6,6-tetramethyl-4-piperidone-N-oxyl (/sup 3/H-TAN) to radiation-induced DNA-transients in E. coli K-12 strains AB 1157 and JO 307 rec A uvr A has been studied under in vivo conditions. After irradiation the cells were washed and resuspended in growth medium and left overnight at 37 deg C. Within an uncertainty of about 10 %, no effect of repair could be detected on the yield of TAN bound to DNA for any of the strains. During the period after resuspension TAN or fragments of TAN leaked out of the irradiated cell samples. This leakage may be attributed to semi-permanent association between TAN and radiation-induced radicals within the cell. The relevance of different interactions between TAN and transients in DNA is discussed.}
journal = {Acta Chem. Scand., Ser. B; (Denmark)}
volume = {29:7}
journal type = {AC}
place = {Denmark}
year = {1975}
month = {Jan}
}