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Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli

Journal Article:

Abstract

A difference was found in the extent of inhibition of thymine dimers (TT) excision in ultraviolet (UV) irradiated cells of E. coli after preirradiation depression of protein and DNA syntheses induced by a simultaneous removal of essential amino acids (AA/sup -/) and thymine (T/sup -/) or by the removal of essential amino acids and the addition of nalidixic acid (NAL/sup +/). The difference was observed in both E. coli B/r Hcr/sup +/ and E. coli K12 SR20 uvr/sup +/ cells. The depression of DNA synthesis by nalidixic acid as an exogenous agent inhibited TT excision to a lower degree than the depression of DNA synthesis by thymine starvation. The extent of TT excision had no appreciable effect on the restoration of the sedimentation profile of a newly synthesized DNA nor on UV resistance of cells during dark repair. A DNA molecule having the size of a molecule of nonirradiated cells became synthesized while TT were still present in the DNA.
Authors:
Masek, F; Slezarikova, V; Sedliakova, M [1] 
  1. Slovenska Akademia Vied, Bratislava (Czechoslovakia). Vyskumny Ustav Onkologicky
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-07-248440; EDB-76-076818
Resource Relation:
Journal Name: Neoplasma; (Czechoslovakia); Journal Volume: 22:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; BIOSYNTHESIS; CONDENSED AROMATICS; DIMERS; ESCHERICHIA COLI; FAR ULTRAVIOLET RADIATION; HETEROCYCLIC ACIDS; INHIBITION; MONOCARBOXYLIC ACIDS; NUTRITIONAL DEFICIENCY; ORGANIC NITROGEN COMPOUNDS; PROTEINS; RADIOSENSITIVITY; THYMINE; AROMATICS; AZINES; BACTERIA; BIOLOGICAL RECOVERY; CARBOXYLIC ACIDS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PYRIMIDINES; RADIATIONS; RECOVERY; REPAIR; SYNTHESIS; ULTRAVIOLET RADIATION; URACILS; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987); 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7179137
Country of Origin:
Serbia and Montenegro
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NEOLA
Submitting Site:
INIS
Size:
Pages: 147-156
Announcement Date:
Jul 01, 1976

Journal Article:

Citation Formats

Masek, F, Slezarikova, V, and Sedliakova, M. Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli. Serbia and Montenegro: N. p., 1975. Web.
Masek, F, Slezarikova, V, & Sedliakova, M. Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli. Serbia and Montenegro.
Masek, F, Slezarikova, V, and Sedliakova, M. 1975. "Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli." Serbia and Montenegro.
@misc{etde_7179137,
title = {Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli}
author = {Masek, F, Slezarikova, V, and Sedliakova, M}
abstractNote = {A difference was found in the extent of inhibition of thymine dimers (TT) excision in ultraviolet (UV) irradiated cells of E. coli after preirradiation depression of protein and DNA syntheses induced by a simultaneous removal of essential amino acids (AA/sup -/) and thymine (T/sup -/) or by the removal of essential amino acids and the addition of nalidixic acid (NAL/sup +/). The difference was observed in both E. coli B/r Hcr/sup +/ and E. coli K12 SR20 uvr/sup +/ cells. The depression of DNA synthesis by nalidixic acid as an exogenous agent inhibited TT excision to a lower degree than the depression of DNA synthesis by thymine starvation. The extent of TT excision had no appreciable effect on the restoration of the sedimentation profile of a newly synthesized DNA nor on UV resistance of cells during dark repair. A DNA molecule having the size of a molecule of nonirradiated cells became synthesized while TT were still present in the DNA.}
journal = {Neoplasma; (Czechoslovakia)}
volume = {22:2}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1975}
month = {Jan}
}