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In situ hybridization studies of hepatitis A viral RNA in patients with acute hepatitis A

Abstract

In situ hybridization with oligonucleotide probes has been used to localise hepatitis A virus RNA genomic sequences in formalin-fixed and routinely processed human liver biopsies from three patients. Using radiolabelled Sulphur-35 antisense probes, viral genomic sequences were found in all three cases, but signal intensity was greatest in cases 1 and 2 with fulminant hepatitis, and was minimal in the third case of resolving hepatitis biopsied 2 months after acute illness. Localisation showed the viral RNA to be present in hepatocytes, sinusoidal cells and inflammatory cells in and around the portal tracts. Both cases showed signal in similar cell types, but the distribution of staining was predominantly periportal in case 1, whereas lobular staining was more apparent in case 2. Hybridization with sense polarity probes failed to detect any evidence of replicative intermediates of antigenomic viral RNA. The presence of hepatitis A RNA in phagocytic cells was confirmed using immunohistochemistryfor a macrophage marker, CD68, combined with in situ hybridization. In all cases the signal was predominantly cytoplasmic, and this was confirmed with the use of tritiated probes. (au).
Authors:
Taylor, M; Goldin, R D; Ladva, S; [1]  Scheuer, P J; [2]  Thomas, H C [3] 
  1. Department of Histopathology, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London (United Kingdom)
  2. Department of Histopathology, Royal Free Hospital and School of Medicine, London (United Kingdom)
  3. Department of Medicine, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London (United Kingdom)
Publication Date:
Jan 01, 1994
Product Type:
Journal Article
Reference Number:
AIX-25-066561; EDB-94-147497
Resource Relation:
Journal Name: Journal of Hepatology (Journal of the European Association for the study of liver); (Netherlands); Journal Volume: 20:3
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; HEPATITIS; VIRUSES; HYBRIDIZATION; BIOPSY; PATIENTS; RNA; SULFUR 35; TRITIUM COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM DISEASES; DISEASES; EVEN-ODD NUCLEI; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; RADIOISOTOPES; SULFUR ISOTOPES; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-); 550601 - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7172002
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0168-8278; CODEN: JOHEEC
Submitting Site:
DKN
Size:
Pages: 380-387
Announcement Date:

Citation Formats

Taylor, M, Goldin, R D, Ladva, S, Scheuer, P J, and Thomas, H C. In situ hybridization studies of hepatitis A viral RNA in patients with acute hepatitis A. Denmark: N. p., 1994. Web. doi:10.1016/S0168-8278(94)80012-X.
Taylor, M, Goldin, R D, Ladva, S, Scheuer, P J, & Thomas, H C. In situ hybridization studies of hepatitis A viral RNA in patients with acute hepatitis A. Denmark. doi:10.1016/S0168-8278(94)80012-X.
Taylor, M, Goldin, R D, Ladva, S, Scheuer, P J, and Thomas, H C. 1994. "In situ hybridization studies of hepatitis A viral RNA in patients with acute hepatitis A." Denmark. doi:10.1016/S0168-8278(94)80012-X. https://www.osti.gov/servlets/purl/10.1016/S0168-8278(94)80012-X.
@misc{etde_7172002,
title = {In situ hybridization studies of hepatitis A viral RNA in patients with acute hepatitis A}
author = {Taylor, M, Goldin, R D, Ladva, S, Scheuer, P J, and Thomas, H C}
abstractNote = {In situ hybridization with oligonucleotide probes has been used to localise hepatitis A virus RNA genomic sequences in formalin-fixed and routinely processed human liver biopsies from three patients. Using radiolabelled Sulphur-35 antisense probes, viral genomic sequences were found in all three cases, but signal intensity was greatest in cases 1 and 2 with fulminant hepatitis, and was minimal in the third case of resolving hepatitis biopsied 2 months after acute illness. Localisation showed the viral RNA to be present in hepatocytes, sinusoidal cells and inflammatory cells in and around the portal tracts. Both cases showed signal in similar cell types, but the distribution of staining was predominantly periportal in case 1, whereas lobular staining was more apparent in case 2. Hybridization with sense polarity probes failed to detect any evidence of replicative intermediates of antigenomic viral RNA. The presence of hepatitis A RNA in phagocytic cells was confirmed using immunohistochemistryfor a macrophage marker, CD68, combined with in situ hybridization. In all cases the signal was predominantly cytoplasmic, and this was confirmed with the use of tritiated probes. (au).}
doi = {10.1016/S0168-8278(94)80012-X}
journal = {Journal of Hepatology (Journal of the European Association for the study of liver); (Netherlands)}
volume = {20:3}
journal type = {AC}
place = {Denmark}
year = {1994}
month = {Jan}
}