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Isolation and characterization of a cellulolytic actinomycete Microbispora bispora

Abstract

Protocols for the isolation of cellulolytic actinomycetes are described, and their use illustrated in the selection of thermophilic bacteria from soil. One isolate, Microbispora bispora, was selected for further study. It grew readily at 55/sup 0/C, produced an extracellular cellulase in good yield (endoglucanase, 5.9 U/ml) that had a broad pH range (pH 5.5 - 7.2) and was thermally stable. Its aryl-..beta..-glucosidase was cell-associated and was relatively resistant to end-product inhibition.
Publication Date:
Sep 01, 1986
Product Type:
Journal Article
Reference Number:
DE-86-013047; EDB-87-006092
Resource Relation:
Journal Name: Appl. Microbiol. Biotechnol.; (Germany, Federal Republic of); Journal Volume: 24:6
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENZYMES; CELLULOLYTIC ACTIVITY; MICROORGANISMS; SCREENING; CELLULASE; CELLULOSE; CULTURE MEDIA; EFFICIENCY; ENZYMATIC HYDROLYSIS; HEMICELLULOSE; PH VALUE; SACCHARIFICATION; THERMOPHILIC CONDITIONS; YIELDS; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; ENZYME ACTIVITY; GLYCOSYL HYDROLASES; HYDROLASES; HYDROLYSIS; LYSIS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; 550700* - Microbiology; 550200 - Biochemistry
OSTI ID:
7170362
Research Organizations:
Rutgers - the State Univ., New Brunswick, NJ, USA. Cook Coll.
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: AMBID
Submitting Site:
DE
Size:
Pages: 477-486
Announcement Date:
Dec 01, 1986

Citation Formats

Waldron, Jr, C R, Becker-Vallone, C A, and Eveleigh, D E. Isolation and characterization of a cellulolytic actinomycete Microbispora bispora. Germany: N. p., 1986. Web.
Waldron, Jr, C R, Becker-Vallone, C A, & Eveleigh, D E. Isolation and characterization of a cellulolytic actinomycete Microbispora bispora. Germany.
Waldron, Jr, C R, Becker-Vallone, C A, and Eveleigh, D E. 1986. "Isolation and characterization of a cellulolytic actinomycete Microbispora bispora." Germany.
@misc{etde_7170362,
title = {Isolation and characterization of a cellulolytic actinomycete Microbispora bispora}
author = {Waldron, Jr, C R, Becker-Vallone, C A, and Eveleigh, D E}
abstractNote = {Protocols for the isolation of cellulolytic actinomycetes are described, and their use illustrated in the selection of thermophilic bacteria from soil. One isolate, Microbispora bispora, was selected for further study. It grew readily at 55/sup 0/C, produced an extracellular cellulase in good yield (endoglucanase, 5.9 U/ml) that had a broad pH range (pH 5.5 - 7.2) and was thermally stable. Its aryl-..beta..-glucosidase was cell-associated and was relatively resistant to end-product inhibition.}
journal = []
volume = {24:6}
journal type = {AC}
place = {Germany}
year = {1986}
month = {Sep}
}