Abstract
The human monoclonal antibody secreted from 4G12 hybridoma cells has broad reactivity to malignant tumor cells, especially for lung squamous cell carcinomas, and recognizes a new tumor-associated and differentiation antigen. The antigen detected by 4G12 is a glycoprotein with MW 195,000 and MW 65,000 under nonreducing and reducing conditions, respectively. Screening of a 4G12 {lambda}gt10 cDNA library with constant region probes for human immunoglobulin yielded full length clones for H- and L-chains. Nucleotide sequences revealed that subtypes of the variable regions were V{sub HIII} and {lambda}{sub 1}, respectively.
Kishimoto, Toshimitsu;
Okajima, Hideki;
Okumoto, Takeki;
[1]
Taniguchi, Masaru
[2]
- Yoshitomi Pharmaceutical Industries, Ltd., Saitama (Japan)
- Chiba Univ. (Japan)
Citation Formats
Kishimoto, Toshimitsu, Okajima, Hideki, Okumoto, Takeki, and Taniguchi, Masaru.
Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody with broad reactivity to malignant tumor cells.
United Kingdom: N. p.,
1989.
Web.
doi:10.1093/nar/17.11.4385.
Kishimoto, Toshimitsu, Okajima, Hideki, Okumoto, Takeki, & Taniguchi, Masaru.
Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody with broad reactivity to malignant tumor cells.
United Kingdom.
https://doi.org/10.1093/nar/17.11.4385
Kishimoto, Toshimitsu, Okajima, Hideki, Okumoto, Takeki, and Taniguchi, Masaru.
1989.
"Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody with broad reactivity to malignant tumor cells."
United Kingdom.
https://doi.org/10.1093/nar/17.11.4385.
@misc{etde_7168481,
title = {Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody with broad reactivity to malignant tumor cells}
author = {Kishimoto, Toshimitsu, Okajima, Hideki, Okumoto, Takeki, and Taniguchi, Masaru}
abstractNote = {The human monoclonal antibody secreted from 4G12 hybridoma cells has broad reactivity to malignant tumor cells, especially for lung squamous cell carcinomas, and recognizes a new tumor-associated and differentiation antigen. The antigen detected by 4G12 is a glycoprotein with MW 195,000 and MW 65,000 under nonreducing and reducing conditions, respectively. Screening of a 4G12 {lambda}gt10 cDNA library with constant region probes for human immunoglobulin yielded full length clones for H- and L-chains. Nucleotide sequences revealed that subtypes of the variable regions were V{sub HIII} and {lambda}{sub 1}, respectively.}
doi = {10.1093/nar/17.11.4385}
journal = []
volume = {17:11}
journal type = {AC}
place = {United Kingdom}
year = {1989}
month = {Jun}
}
title = {Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody with broad reactivity to malignant tumor cells}
author = {Kishimoto, Toshimitsu, Okajima, Hideki, Okumoto, Takeki, and Taniguchi, Masaru}
abstractNote = {The human monoclonal antibody secreted from 4G12 hybridoma cells has broad reactivity to malignant tumor cells, especially for lung squamous cell carcinomas, and recognizes a new tumor-associated and differentiation antigen. The antigen detected by 4G12 is a glycoprotein with MW 195,000 and MW 65,000 under nonreducing and reducing conditions, respectively. Screening of a 4G12 {lambda}gt10 cDNA library with constant region probes for human immunoglobulin yielded full length clones for H- and L-chains. Nucleotide sequences revealed that subtypes of the variable regions were V{sub HIII} and {lambda}{sub 1}, respectively.}
doi = {10.1093/nar/17.11.4385}
journal = []
volume = {17:11}
journal type = {AC}
place = {United Kingdom}
year = {1989}
month = {Jun}
}