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MPO cDNA clone identifies an RFLP with PstI

Abstract

A myeloperoxidase (MPO) cDNA clone (pHMP7: 270 base pair insert in the vector pGEM-1reverse arrow was isolated from a library created from human promyelocytic (HL-60) cell mRNA. PstI (CTGCA/G) (New England Biolabs) identifies a simple two-allele polymorphism with bands at either 2.2 kb (Al) or 2.0 kb (A2). There are three constant bands at 2.8 kb, 0.95 kb and 0.6 kb. Preliminary family data show evidence of linkage to several markers in proximal 17q, with MPO closest to the Growth Hormone cluster at 17q22-q24. Autosomal condominant segregation was observed in four large reference pedigrees with several informative matings.
Publication Date:
Feb 25, 1988
Product Type:
Journal Article
Reference Number:
EDB-88-143432
Resource Relation:
Journal Name: Nucleic Acids Res.; (United Kingdom); Journal Volume: 16:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEROXIDASES; DNA-CLONING; RECOMBINANT DNA; DNA SEQUENCING; HUMAN POPULATIONS; MAN; MESSENGER-RNA; RFLPS; ANIMALS; CLONING; DNA; ENZYMES; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; POPULATIONS; PRIMATES; RNA; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550200* - Biochemistry
OSTI ID:
7164763
Research Organizations:
Yale Univ. School of Medicine, New Haven, CT (USA)
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NARHA
Submitting Site:
JMT
Size:
Pages: 1649
Announcement Date:
Aug 01, 1988

Citation Formats

Miki, T, Weil, S C, Rosner, G L, Reid, M S, and Kidd, K K. MPO cDNA clone identifies an RFLP with PstI. United Kingdom: N. p., 1988. Web. doi:10.1093/nar/16.4.1649.
Miki, T, Weil, S C, Rosner, G L, Reid, M S, & Kidd, K K. MPO cDNA clone identifies an RFLP with PstI. United Kingdom. https://doi.org/10.1093/nar/16.4.1649
Miki, T, Weil, S C, Rosner, G L, Reid, M S, and Kidd, K K. 1988. "MPO cDNA clone identifies an RFLP with PstI." United Kingdom. https://doi.org/10.1093/nar/16.4.1649.
@misc{etde_7164763,
title = {MPO cDNA clone identifies an RFLP with PstI}
author = {Miki, T, Weil, S C, Rosner, G L, Reid, M S, and Kidd, K K}
abstractNote = {A myeloperoxidase (MPO) cDNA clone (pHMP7: 270 base pair insert in the vector pGEM-1reverse arrow was isolated from a library created from human promyelocytic (HL-60) cell mRNA. PstI (CTGCA/G) (New England Biolabs) identifies a simple two-allele polymorphism with bands at either 2.2 kb (Al) or 2.0 kb (A2). There are three constant bands at 2.8 kb, 0.95 kb and 0.6 kb. Preliminary family data show evidence of linkage to several markers in proximal 17q, with MPO closest to the Growth Hormone cluster at 17q22-q24. Autosomal condominant segregation was observed in four large reference pedigrees with several informative matings.}
doi = {10.1093/nar/16.4.1649}
journal = []
volume = {16:4}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Feb}
}