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Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells

Journal Article:

Abstract

Peripheral blood mononuclear cells (PBMC) from healthy donors and AML patients in remission were stimulated with phytohemagglutinin (PHA) and recombinant interleukin-2 (IL-2). These stimulated cells (lymphokine activated killer (LAK) cells) showed increased DNA synthesis as measured by /sup 3/H-Thymidine uptake. A synergistic effect of PHA and IL-2 was found. LAK cells' ability to kill acute myeloid leukemia (AML) blasts was investigated by the /sup 51/Cr release assay. LAK cells showed a cytotoxicity (over 10% specific /sup 51/Cr release) against 9/12 leukemic blasts, even at effector/target (E/T) ratios as low as 5:1. However, on average only 22.2% (SD 11.8) and 36.5% (SD 12.5) /sup 51/Cr release were obtained in 4- and 18-hour cytotoxicity assays, respectively, at an E/T ratio of 20:1. Leukemic blasts in 3/12 AML cases and normal PBMC were entirely resistant to lysis, even at an E/T ratio of 80:1. Susceptibility to lysis was not correlated to peanut-agglutinin receptor expression. LAK cells were more cytotoxic towards the K-562 cell line (natural killer activity) than unstimulated PBMC.
Publication Date:
Jan 01, 1988
Product Type:
Journal Article
Reference Number:
NORN-88-000117; EDB-88-144658
Resource Relation:
Journal Name: Eur. J. Haematol.; (Denmark); Journal Volume: 40
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEUKEMIA; CELL KILLING; LYMPHOKINES; CHROMIUM 51; DECOMPOSITION; PHYTOHEMAGGLUTININ; TRITIUM COMPOUNDS; AGGLUTININS; ANTIBODIES; BETA DECAY RADIOISOTOPES; CARBOHYDRATES; CHEMICAL REACTIONS; CHROMIUM ISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; GROWTH FACTORS; HEMAGGLUTININS; HEMIC DISEASES; IMMUNE SYSTEM DISEASES; INTERMEDIATE MASS NUCLEI; ISOTOPES; LABELLED COMPOUNDS; MITOGENS; MUCOPROTEINS; NEOPLASMS; NUCLEI; ORGANIC COMPOUNDS; POLYSACCHARIDES; PROTEINS; RADIOISOTOPES; SACCHARIDES; 560161* - Radionuclide Effects, Kinetics, & Toxicology- Man
OSTI ID:
7161211
Research Organizations:
Division of Hematology, Karolinska Hospital, Stockholm (SE); SE; Central Microbiological Lab., Stockholm County Council, Stockholm (SE)
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EJHAE
Submitting Site:
DKN
Size:
Pages: 362-367
Announcement Date:

Journal Article:

Citation Formats

Panayotides, P, Sjoegren, A -M, Reizenstein, P, Porwit, A. Immunopathology Lab., Dept. of Pathology, Karolinska Hospital, Stockholm, and Wasserman, J. Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells. Denmark: N. p., 1988. Web.
Panayotides, P, Sjoegren, A -M, Reizenstein, P, Porwit, A. Immunopathology Lab., Dept. of Pathology, Karolinska Hospital, Stockholm, & Wasserman, J. Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells. Denmark.
Panayotides, P, Sjoegren, A -M, Reizenstein, P, Porwit, A. Immunopathology Lab., Dept. of Pathology, Karolinska Hospital, Stockholm, and Wasserman, J. 1988. "Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells." Denmark.
@misc{etde_7161211,
title = {Resistance of some leukemic blasts to lysis by lymphokine activated killer (LAK) cells}
author = {Panayotides, P, Sjoegren, A -M, Reizenstein, P, Porwit, A. Immunopathology Lab., Dept. of Pathology, Karolinska Hospital, Stockholm, and Wasserman, J}
abstractNote = {Peripheral blood mononuclear cells (PBMC) from healthy donors and AML patients in remission were stimulated with phytohemagglutinin (PHA) and recombinant interleukin-2 (IL-2). These stimulated cells (lymphokine activated killer (LAK) cells) showed increased DNA synthesis as measured by /sup 3/H-Thymidine uptake. A synergistic effect of PHA and IL-2 was found. LAK cells' ability to kill acute myeloid leukemia (AML) blasts was investigated by the /sup 51/Cr release assay. LAK cells showed a cytotoxicity (over 10% specific /sup 51/Cr release) against 9/12 leukemic blasts, even at effector/target (E/T) ratios as low as 5:1. However, on average only 22.2% (SD 11.8) and 36.5% (SD 12.5) /sup 51/Cr release were obtained in 4- and 18-hour cytotoxicity assays, respectively, at an E/T ratio of 20:1. Leukemic blasts in 3/12 AML cases and normal PBMC were entirely resistant to lysis, even at an E/T ratio of 80:1. Susceptibility to lysis was not correlated to peanut-agglutinin receptor expression. LAK cells were more cytotoxic towards the K-562 cell line (natural killer activity) than unstimulated PBMC.}
journal = {Eur. J. Haematol.; (Denmark)}
volume = {40}
journal type = {AC}
place = {Denmark}
year = {1988}
month = {Jan}
}