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Endonuclease activities in extracts of Micrococcus luteus that act on. gamma. -irradiated DNA

Abstract

Several protein fractions containing endonuclease activity against ..gamma..-irradiated DNA (..gamma..-endonuclease) were isolated from M.luteus. The crude extract was eluted on a phosphocellulose column and chromatographed on TEAE cellulose and subsequently on hydroxypatite. Five peaks of ..gamma..-endonuclease were obtained from each preparation. Repeated experiments showed comparable chromatographic behaviour of the fractions. There was no detectable activity of uv-endonuclease in the fractions with ..gamma..-endonuclease but a small contamination of endonuclease against unirradiated DNA and against DNA with apurinic sites. The ..gamma..-endonuclease was stimulated by, but was not dependent on, magnesium. Several tests for endonuclease activity have been used: the analysis of strand breaks in calf-thymus DNA or in PM2 DNA, and the determination of end-groups formed by endonuclease, either 3'OH end-groups or phosphomonoester end groups. From the results obtained it can be assumed that the strand breaks induced by the ..gamma..-endonuclease carry 3'OH and 5' phosphate end groups.
Authors:
Schoen-Bopp, A; Schaefer, G; Hagen, U [1] 
  1. Kernforschungszentrum Karlsruhe (Germany, F.R.). Inst. fuer Strahlenbiologie
Publication Date:
Mar 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-321390; EDB-77-121917
Resource Relation:
Journal Name: Int. J. Radiat. Biol.; (United Kingdom); Journal Volume: 31:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; STRAND BREAKS; NUCLEASES; CHEMICAL RADIATION EFFECTS; CHROMATOGRAPHY; GAMMA RADIATION; MICROCOCCUS; MOLECULAR STRUCTURE; PHOSPHORIC ACID ESTERS; BACTERIA; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERS; IONIZING RADIATIONS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHOTRANSFERASES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; SEPARATION PROCESSES; TRANSFERASES; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987); 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7142859
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 227-235
Announcement Date:

Citation Formats

Schoen-Bopp, A, Schaefer, G, and Hagen, U. Endonuclease activities in extracts of Micrococcus luteus that act on. gamma. -irradiated DNA. United Kingdom: N. p., 1977. Web. doi:10.1080/09553007714550271.
Schoen-Bopp, A, Schaefer, G, & Hagen, U. Endonuclease activities in extracts of Micrococcus luteus that act on. gamma. -irradiated DNA. United Kingdom. doi:10.1080/09553007714550271.
Schoen-Bopp, A, Schaefer, G, and Hagen, U. 1977. "Endonuclease activities in extracts of Micrococcus luteus that act on. gamma. -irradiated DNA." United Kingdom. doi:10.1080/09553007714550271. https://www.osti.gov/servlets/purl/10.1080/09553007714550271.
@misc{etde_7142859,
title = {Endonuclease activities in extracts of Micrococcus luteus that act on. gamma. -irradiated DNA}
author = {Schoen-Bopp, A, Schaefer, G, and Hagen, U}
abstractNote = {Several protein fractions containing endonuclease activity against ..gamma..-irradiated DNA (..gamma..-endonuclease) were isolated from M.luteus. The crude extract was eluted on a phosphocellulose column and chromatographed on TEAE cellulose and subsequently on hydroxypatite. Five peaks of ..gamma..-endonuclease were obtained from each preparation. Repeated experiments showed comparable chromatographic behaviour of the fractions. There was no detectable activity of uv-endonuclease in the fractions with ..gamma..-endonuclease but a small contamination of endonuclease against unirradiated DNA and against DNA with apurinic sites. The ..gamma..-endonuclease was stimulated by, but was not dependent on, magnesium. Several tests for endonuclease activity have been used: the analysis of strand breaks in calf-thymus DNA or in PM2 DNA, and the determination of end-groups formed by endonuclease, either 3'OH end-groups or phosphomonoester end groups. From the results obtained it can be assumed that the strand breaks induced by the ..gamma..-endonuclease carry 3'OH and 5' phosphate end groups.}
doi = {10.1080/09553007714550271}
journal = {Int. J. Radiat. Biol.; (United Kingdom)}
volume = {31:3}
journal type = {AC}
place = {United Kingdom}
year = {1977}
month = {Mar}
}