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Radioimmunoassay of synthetic steroids

Abstract

The sensitivity of a radioimmunoassay depends on the intrinsic association constant of the interaction between ligand and antibody. Its specificity depends on the position of the chain which forms the link with the antigen. Thus, an antibody specific of estradiol has been obtained by coupling estradiol to albumin via a chain at position 7. For synthetic steroids the structure of which is sufficiency different from that of natural hormones, the requirements for a sensitive assay method not involving chromatography are simply maximum affinity and positioning of the couple at a site which does not undergo metabolic attack. These criteria were used to develop assays for R 2858 and R 2453 which obviate the need to administer radioactive product in clinical pharmacology. Cross-reaction with structural analogs may be used to assay competitors. Thus, R 2323 antibody, highly specific for endogenous steroids, may be used to assay other trienes such as R 1697 (trenbolone) and R 2010 (norgestrienone).
Publication Date:
Dec 01, 1975
Product Type:
Conference
Reference Number:
AIX-07-260383; EDB-77-014388
Resource Relation:
Journal Name: Pathol.-Biol.; (France); Journal Volume: 23:10; Conference: 2. Colloquium of radioimmunology, Lyon, France, 25 Oct 1974
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; STEROIDS; RADIOIMMUNOASSAY; ANTIGEN-ANTIBODY REACTIONS; IMMUNE SERUMS; IODINE 125; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7139654
Country of Origin:
France
Language:
French
Other Identifying Numbers:
Journal ID: CODEN: PABIA
Submitting Site:
INIS
Size:
Pages: 895-901
Announcement Date:

Citation Formats

Raynaud, J -P, Bucourt, R, and Salmon, J. Radioimmunoassay of synthetic steroids. France: N. p., 1975. Web.
Raynaud, J -P, Bucourt, R, & Salmon, J. Radioimmunoassay of synthetic steroids. France.
Raynaud, J -P, Bucourt, R, and Salmon, J. 1975. "Radioimmunoassay of synthetic steroids." France.
@misc{etde_7139654,
title = {Radioimmunoassay of synthetic steroids}
author = {Raynaud, J -P, Bucourt, R, and Salmon, J}
abstractNote = {The sensitivity of a radioimmunoassay depends on the intrinsic association constant of the interaction between ligand and antibody. Its specificity depends on the position of the chain which forms the link with the antigen. Thus, an antibody specific of estradiol has been obtained by coupling estradiol to albumin via a chain at position 7. For synthetic steroids the structure of which is sufficiency different from that of natural hormones, the requirements for a sensitive assay method not involving chromatography are simply maximum affinity and positioning of the couple at a site which does not undergo metabolic attack. These criteria were used to develop assays for R 2858 and R 2453 which obviate the need to administer radioactive product in clinical pharmacology. Cross-reaction with structural analogs may be used to assay competitors. Thus, R 2323 antibody, highly specific for endogenous steroids, may be used to assay other trienes such as R 1697 (trenbolone) and R 2010 (norgestrienone).}
journal = {Pathol.-Biol.; (France)}
volume = {23:10}
place = {France}
year = {1975}
month = {Dec}
}