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Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies

Abstract

Human trophoblast membrane antigens recognised by monoclonal antibodies (H310, H315, H316 and H317) have been identified using combinations of radioimmunoprecipitation, SDS-PAGE, electroblotting, chromatographic and ELISA-type techniques. H317 is known to identify heat-stable placental-type alkaline phosphatase and accordingly was shown to react with a protein of subunit Msub(r) of 68000. H310 and H316 both recognise an antigen with a subunit Msub(r) of 34000 under reducing conditions. In non-reducing conditions, the H310/316 antigen gave oligomers of a component of Msub(r) 62000. It is unknown whether this 62000 dalton component is a dimer of the 34000 dalton protein with either itself or a second protein chain of presumed Msub(r) around 28000. H315 recognises an antigen with subunit Msub(r) of 36000; in non-reducing conditions this component readily associates to oligomeric structures. The epitope recognised by H315 may be sensitive to SDS. The two proteins recognised by H310/316 and H315 have been termed the p34 and p36 trophoblast membrane proteins, respectively.
Authors:
Brown, P J; Molloy, C M; Johnson, P M [1] 
  1. Liverpool Univ. (UK). Dept. of Immunology
Publication Date:
Nov 01, 1983
Product Type:
Journal Article
Reference Number:
AIX-15-024636; EDB-84-086302
Resource Relation:
Journal Name: J. Reprod. Immunol.; (Netherlands); Journal Volume: 5:6
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CELL MEMBRANES; IMMUNOLOGY; ANTIGENS; ELECTROPHORESIS; GAMMA DETECTION; GELS; IODINE 125; LABELLED COMPOUNDS; MONOCLONAL ANTIBODIES; PRECIPITATION; PROTEINS; ANTIBODIES; BETA DECAY RADIOISOTOPES; CELL CONSTITUENTS; COLLOIDS; DAYS LIVING RADIOISOTOPES; DETECTION; DISPERSIONS; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIATION DETECTION; RADIOISOTOPES; SEPARATION PROCESSES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7134551
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JRIMD
Submitting Site:
HEDB
Size:
Pages: 351-361
Announcement Date:

Citation Formats

Brown, P J, Molloy, C M, and Johnson, P M. Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies. Netherlands: N. p., 1983. Web. doi:10.1016/0165-0378(83)90244-9.
Brown, P J, Molloy, C M, & Johnson, P M. Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies. Netherlands. doi:10.1016/0165-0378(83)90244-9.
Brown, P J, Molloy, C M, and Johnson, P M. 1983. "Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies." Netherlands. doi:10.1016/0165-0378(83)90244-9. https://www.osti.gov/servlets/purl/10.1016/0165-0378(83)90244-9.
@misc{etde_7134551,
title = {Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies}
author = {Brown, P J, Molloy, C M, and Johnson, P M}
abstractNote = {Human trophoblast membrane antigens recognised by monoclonal antibodies (H310, H315, H316 and H317) have been identified using combinations of radioimmunoprecipitation, SDS-PAGE, electroblotting, chromatographic and ELISA-type techniques. H317 is known to identify heat-stable placental-type alkaline phosphatase and accordingly was shown to react with a protein of subunit Msub(r) of 68000. H310 and H316 both recognise an antigen with a subunit Msub(r) of 34000 under reducing conditions. In non-reducing conditions, the H310/316 antigen gave oligomers of a component of Msub(r) 62000. It is unknown whether this 62000 dalton component is a dimer of the 34000 dalton protein with either itself or a second protein chain of presumed Msub(r) around 28000. H315 recognises an antigen with subunit Msub(r) of 36000; in non-reducing conditions this component readily associates to oligomeric structures. The epitope recognised by H315 may be sensitive to SDS. The two proteins recognised by H310/316 and H315 have been termed the p34 and p36 trophoblast membrane proteins, respectively.}
doi = {10.1016/0165-0378(83)90244-9}
journal = {J. Reprod. Immunol.; (Netherlands)}
volume = {5:6}
journal type = {AC}
place = {Netherlands}
year = {1983}
month = {Nov}
}