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Cellular localization of peptide hydrolases in chicken embryo tissues and influence of gamma irradiation on their activity

Abstract

Studied was the influence of chicken embryo irradiation at 600 R and 1000 R gamma rays on the activity of tissue peptide hydrolases in mitochondrial-lysosomal, microsomal and supernatant (cell hyaloplasm) cell fractions. The investigation was performed 50 to 168 hours post irradiation. The wole tissue (of the whole embryo) was examined following irradiation of 4-day-old embryos whose liver, muscle and brain tissues were post irradiation examined on day 12 and 16 of incubation. Prior to treatment, the tissues were threfold rinsed with sucrose solution to eliminate proeinase inhibitors. Lysosome membranes were destroyed by adding 0.5 % desoxycholate. It was found that: Peptide hydrolase activity of mitochondrial-lysosomal cell fractions of tissues of whole 6-day chicken embryos is 4-5 times as high as that of cell hyaloplasm. Peptide hydrolase activity of mitochondrial-lysosomal fractions of liver tissues decreases on day 18 and 19 post incubation, while the same fraction of muscle and brain tissues shows high activity. Peptide hydrolase activity of microsomal fraction and of cell hyaloplasm rises during embryonal development and exceeds the activity of liver tissue mitochondrial fraction. Peptide hydrolase activity of mitochondrial-lysosomal fraction of tissue of whole 6-day-old embryos 50 hours post irradiation is higher than the activity of non-irradiated  More>>
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
AIX-08-293247; EDB-77-052088
Resource Relation:
Journal Name: God. Sofii. Univ., Biol. Fak.; (Bulgaria); Journal Volume: 67
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; PEPTIDE HYDROLASES; BIOLOGICAL RADIATION EFFECTS; BRAIN; CHICKENS; EMBRYOS; ENZYMES; GAMMA RADIATION; LIVER; LYSOSOMES; MITOCHONDRIA; MUSCLES; TISSUES; ANIMALS; BIOLOGICAL EFFECTS; BIRDS; BODY; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; DIGESTIVE SYSTEM; ELECTROMAGNETIC RADIATION; FOWL; GLANDS; HYDROLASES; IONIZING RADIATIONS; NERVOUS SYSTEM; ORGANOIDS; ORGANS; RADIATION EFFECTS; RADIATIONS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals
OSTI ID:
7126103
Country of Origin:
Bulgaria
Language:
Bulgarian
Other Identifying Numbers:
Journal ID: CODEN: GSBIA
Submitting Site:
INIS
Size:
Pages: 227-234
Announcement Date:
Mar 01, 1977

Citation Formats

Khristov, D, and Marinopolski, G. Cellular localization of peptide hydrolases in chicken embryo tissues and influence of gamma irradiation on their activity. Bulgaria: N. p., 1975. Web.
Khristov, D, & Marinopolski, G. Cellular localization of peptide hydrolases in chicken embryo tissues and influence of gamma irradiation on their activity. Bulgaria.
Khristov, D, and Marinopolski, G. 1975. "Cellular localization of peptide hydrolases in chicken embryo tissues and influence of gamma irradiation on their activity." Bulgaria.
@misc{etde_7126103,
title = {Cellular localization of peptide hydrolases in chicken embryo tissues and influence of gamma irradiation on their activity}
author = {Khristov, D, and Marinopolski, G}
abstractNote = {Studied was the influence of chicken embryo irradiation at 600 R and 1000 R gamma rays on the activity of tissue peptide hydrolases in mitochondrial-lysosomal, microsomal and supernatant (cell hyaloplasm) cell fractions. The investigation was performed 50 to 168 hours post irradiation. The wole tissue (of the whole embryo) was examined following irradiation of 4-day-old embryos whose liver, muscle and brain tissues were post irradiation examined on day 12 and 16 of incubation. Prior to treatment, the tissues were threfold rinsed with sucrose solution to eliminate proeinase inhibitors. Lysosome membranes were destroyed by adding 0.5 % desoxycholate. It was found that: Peptide hydrolase activity of mitochondrial-lysosomal cell fractions of tissues of whole 6-day chicken embryos is 4-5 times as high as that of cell hyaloplasm. Peptide hydrolase activity of mitochondrial-lysosomal fractions of liver tissues decreases on day 18 and 19 post incubation, while the same fraction of muscle and brain tissues shows high activity. Peptide hydrolase activity of microsomal fraction and of cell hyaloplasm rises during embryonal development and exceeds the activity of liver tissue mitochondrial fraction. Peptide hydrolase activity of mitochondrial-lysosomal fraction of tissue of whole 6-day-old embryos 50 hours post irradiation is higher than the activity of non-irradiated embryos. Later the activity of this fraction diminishes and on the 168 hr post irradiation it drops below the normal. Microsomal fraction and cell hyaloplasm activity likewise show deviation from the norm. Peptide hydrolase activity of mitochondrial-lysosomal fraction of liver, muscle and brain tissue of 14 and 18-day-old embryos is higher than the control 50 hours post irradiation and then declines. The activity of mitochondrial-lysosomal fraction of embryo brain tissue changes most strikingly on irradiation, while other brain cell fractions change less compared with liver and muscle fractions.}
journal = []
volume = {67}
journal type = {AC}
place = {Bulgaria}
year = {1975}
month = {Jan}
}